20 research outputs found
How vulnerable are cetaceans to climate change? Developing and testing a new index
info:eu-repo/semantics/publishedVersio
A whole genome screen for association with multiple sclerosis in portuguese patients
Multiple sclerosis (MS) is common in Europe affecting up to 1:500 people. In an effort to identify genes influencing susceptibility
to the disease, we have performed a population-based whole genome screen for association. In this study, 6000 microsatellite markers
were typed in separately pooled DNA samples from MS patients (n = 188) and matched controls (n = 188). Interpretable data was
obtained from 4661 of these markers. Refining analysis of the most promising markers identified 10 showing potential evidence for
association.SERONO (Portugal).Fundação para a Ciência e a Tecnologia (FCT) - grant FRH/BD/9111/2002.British Council/ICCTI.Wellcome Trust, Multiple Sclerosis Societies of the United States and Great Britain, Multiple Sclerosis International Federation - GAMES project - grant 057097
Effects of Trihalomethanes on Liver Mitochondria
Introduction: Trihalomethanes (THMs), namely dibromochloromethane
(DBCM) and bromodichloromethane (BDCM), are disinfection
byproducts of chlorinated water. This experiment aimed to
evaluate the mitochondrial dysfunction induced by THMs at low
levels in a mouse model
Guillain-Barré syndrome: the reality of an Hospital Center
Introdução – O Síndrome Guillain-Barré (SGB) é uma polineuropatia desmielinizante
aguda de natureza auto-imune.
Objectivo – Caracterizar a população
pediátrica de doentes internados no
Centro Hospitalar de Vila Nova de Gaia
com o diagnóstico de SGB, quanto à forma
de apresentação, tratamento e evolução
clínica.
Doentes e Métodos – Foram analisados
os processos clínicos dos doentes
com idade inferior a 16 anos com o diagnóstico de SGB, entre 1 Janeiro de 2000
e 31 Dezembro de 2006. Aplicou-se na
avaliação do estado funcional a Escala
de Van Der Merché e Van Doorn.
Resultados – Foram estudados sete
casos, quatro do sexo masculino, com
idades entre os 2 e 10 anos. Em 71% dos
doentes a infecção respiratória ou gastrointestinal
precedeu o quadro. Todos apresentaram
alterações motoras, cinco alterações
sensitivas e três envolvimento dos pares
cranianos. Em três a forma de apresentação
foi atípica. Cinco crianças tinham
dissociação albumino-citológica (DAC) e
encontraram-se sinais electromiográficos
de polineuropatia desmielinizante aguda
em 3. O agente mais frequentemente implicado
foi o Campylobacter jejuni. Todas
foram tratadas com imunoglobulina endovenosa
(IgEV), com boa evolução clínica,
recuperando sem sequelas. À altura da
alta a maioria apresentava incapacidade
funcional moderada.
Discussão – A forma de apresentação
nem sempre é a típica, podendo manifestar-se por dores musculares, simulando
uma miosite, por forma “encefaliticas” ou
por afecção dos pares cranianos. A punção
lombar e a electromiografia (EMG) são
bons exames auxiliares de diagnóstico.
Contudo, quando a punção é realizada muito
precocemente poderão não se encontrar
as alterações esperadas; da mesma forma,
a EMG deverá ser realizada depois das
três semanas para ser informativa. ABSTRACT
Introduction - Guillain-Barré Syndrome
(GBS) is an acute autoimmune
demyelinating polyneuropatia. The aim of
this work was to characterize the pediatric
patients admitted to Centro Hospitalar
Vila Nova de Gaia with GBS, analyzing
the clinical presentation, treatment and
the clinical evolution.
Patients and Methods - Retrospective
review of all children less than 16
years with GBS admitted in Centro Hospitalar
de Vila Nova de Gaia between 1st
January 2000 and 31st December 2006.
The functional state was evaluated by the
Van Der Merché and Van Doorn scale.
Results - Seven cases were admitted
during this period, 4 were males, aged
2 to 10 years. Respiratory and gastrointestinal
prodromes were identified in 71%
of the cases. All patients had motor disturbances,
5 sensory manifestations and 3
cranial nerves involvement. Five children
had albuminocytological dissociation and
electromyographic signs of acute demyelinating
neuropathy was identifi ed in 3.
Campylobacter jejuni was the agent more
commonly involved. All children were
treated with intravenous imunoglobulins,
with good evolution, recovering without
sequels. On the time of discharge most of
the children presented a moderate functional
incapacity.
Discussion - The clinical presentation
in GBS can be variable: classic, miosites-like or encephalitis-like or with craneal
nerve envolvent. Lumbar puncture
(LP) and electromyogram are important to support the diagnosis. Nevertheless
when LP is performed early in the course
of the disease typical abnormalities may
not be present. Also electrophysiological
studies should be made after the third
week of disease to be of greater diagnostic
value
Youth clinics in a health care center – nine years experience
RESUMO
Introdução e objectivos: Os Centros
de Atendimento a Jovens (CAJ) foram
criados com o objectivo de suprir
uma lacuna na relação, em termos de
saúde, com os jovens portugueses. O
objectivo deste estudo foi analisar as características
dos utentes do CAJ e o tipo
de utilização dos serviços de saúde.
Material e métodos: Foi realizado
um estudo retrospectivo descritivo dos
registos do CAJ num Centro de Saúde
do Norte de Portugal nos anos de 2000
a 2008, avaliando variáveis epidemiológicas
e de utilização do centro.
Resultados: Entre 2000-2008
realizaram-se 2024 primeiras e 10889
segundas consultas; as consultas a jovens
do sexo feminino quintuplicaram
de 2000 para 2004, tendo-se mantido
estáveis para o sexo feminino de 2005
a 2008 e para o sexo masculino de 2000
a 2008.
Os principais motivos de recurso ao
CAJ foram o início e manutenção da contracepção
(47 a 63%), informação geral e
sobre sexualidade (19 a 48%). A contracepção
de emergência, disponível desde
2002, foi dispensada nesta consulta a 168
utentes até ao ano de 2008. Nos anos
analisados, verificou-se que 68% das
utentes que recorreram à contracepção
de emergência tinham acompanhamento
habitual pelo seu médico de família ou no
CAJ; a sua necessidade deveu-se, em
cerca de 51% dos casos, a relação sexual
não protegida e nos restantes 49% a
falência do método contraceptivo.
Conclusão: A utilização do CAJ
tem aumentado ao longo dos anos. A vertente
de planeamento familiar foi a mais
procurada. O início da vida sexual já tinha
ocorrido na maioria dos casos antes
do recurso à consulta, e de uma forma
desprotegida numa proporção preocupante.
O escasso número de rapazes foi
sugestivo de uma atitude de desresponsabilização
masculina relativamente à
contracepção.
ABSTRACT
Introduction and aim: The youth
clinics (YC) were created to promote an
age oriented health care services. The
objective of this study was to characterize
the population attending an YC and
the type of utilization of the health care
services.
Methods: Retrospective descriptive
study of a Health Care Center YC
in Northern Portugal, between the years
2000 and 2008, with analysis of epidemiologic
and utilization characteristics of
its population.
Results: Between 2000 and 2008
we had 2024 first and 10889 subsequent
appointments; consultations for females
increased five times between 2000-2004,
becoming stable thereafter; the number
of consultations for males was similar
each year.
The main reasons to attend an YC
were the start and maintenance of contraception
(47-63%) and to get general
health and sexuality-related information
(19-48%). Emergency contraception,
available since 2002, was given to 168
women until 2008; 68% of them had regular
medical follow-up at her family doctor
or at YC; this method was searched
because of previous unprotected sexual
intercourse (51%) or failure of the contraceptive
method used during the intercourse
(49%).
Conclusions: YC utilization has
been growing up steadily. Contraception
search was the main appointment reason.
Sex life had already started before
the first appointment in most cases, and
with no kind of protection in considerable
number of users. The small number of
males attending YC might be regarded as
a non-responsible attitude towards contraception
Dermatology case: scabies
The authors describe a clinical case of a 5-months-old boy
with widespread rash since the first month of life. Diffuse papular rash with intensive itching. The shaved skin was inconclusive so we performed biopsy to confirm the diagnosis of scabies. It’s a dermatosis very contagious caused by the mite Sarcoptes scabiei var. hominis. In infants skin eruption may have a polymorphic presentation with characteristic distribution
The coal cleat system: A new approach to its study
AbstractAfter a general analysis regarding the concept of coal “cleat system”, its genetic origin and practical applications to coalbed methane (CBM) commercial production and to CO2 geological sequestration projects, the authors have developed a method to answer, quickly and accurately in accordance with the industrial practice and needs, the following yet unanswered questions: (1) how to define the spatial orientation of the different classes of cleats presented in a coal seam and (2) how to determine the frequency of their connectivites. The new available and presented techniques to answer these questions have a strong computer based tool (geographic information system, GIS), able to build a complete georeferentiated database, which will allow to three-dimensionally locate the laboratory samples in the coalfield. It will also allow to better understand the coal cleat system and consequently to recognize the best pathways to gas flow through the coal seam. Such knowledge is considered crucial for understanding what is likely to be the most efficient opening of cleat network, then allowing the injection with the right spatial orientation, of pressurized fluids in order to directly drain the maximum amount of gas flow to a CBM exploitation well. The method is also applicable to the CO2 geological sequestration technologies and operations corresponding to the injection of CO2 sequestered from industrial plants in coal seams of abandoned coal mines or deep coal seams
Modification of wheat straw lignin by solid state fermentation with white-rot fungi
The potential of crude enzyme extracts, obtained from solid state cultivation of four white-rot fungi (Trametes versicolor, Bjerkandera adusta, Ganoderma applanatum and Phlebia rufa), was exploited to modify wheat straw cell wall. At different fermentation times, manganese-dependent peroxidase (MnP), lignin peroxidase (LiP), laccase, carboxymethylcellulase (CMCase), avicelase, xylanase and feruloyl esterase activities were screened and the content of lignin as well as hydroxycinnamic acids in fermented straw were determined. All fungi secreted feruloyl esterase while LiP was only detected in crude extracts from B. adusta. Since no significant differences (P > 0.05) were observed in remaining lignin content of fermented straw, LiP activity was not a limiting factor of enzymatic lignin removal process. The levels of esterified hydroxycinnamic acids degradation were considerably higher than previous reports with lignocellulosic biomass. The data show that P. rufa, may be considered for more specific studies as higher ferulic and p-coumaric acids degradation was observed for earlier incubation time
Deposição atmosférica de poeiras e níveis de elementos traço numa cidade industrial no litoral centro de Portugal
International audienceO presente estudo pretendeu (i) identificar padrões geoquímicos espaciais e temporais na deposição atmosférica, e (ii) avaliar potenciais contribuições de fontes locais e alóctones para a composição química das poeiras atmosféricas no concelho de Estarreja. Dois coletores foram colocados em áreas exteriores de duas escolas localizadas poucos quilómetros a sul da zona industrial. A recolha das amostras foi realizada mensalmente, de maio a agosto de 2018. As frações solúvel e insolúvel da deposição atmosférica foram analisadas por ICP-MS para determinar as concentrações quase-totais dos elementos químicos. Os resultados mostram a ocorrência de dois eventos distintos de maior fluxo de deposição atmosférica: (i) um em maio, caracterizado pela deposição de elementos traço em formas solúveis, detetado em ambas as escolas e (ii) um segundo em agosto, caracterizado pela deposição de elementos maiores em formas insolúveis, detetado apenas na escola mais distante do complexo industrial. No geral, os resultados indicam uma deposição antrópica de aerossóis em maio e uma deposição de partículas litogénicas em agosto