Neural regulation of cardiovascular response to exercise: role of central command and peripheral afferents

During dynamic exercise, mechanisms controlling the cardiovascular apparatus operate to provide adequate oxygen to fulfill metabolic demand of exercising muscles and to guarantee metabolic end-products washout. Moreover, arterial blood pressure is regulated to maintain adequate perfusion of the vital organs without excessive pressure variations. The autonomic nervous system adjustments are characterized by a parasympathetic withdrawal and a sympathetic activation. In this review, we briefly summarize neural reflexes operating during dynamic exercise. The main focus of the present review will be on the central command, the arterial baroreflex and chemoreflex, and the exercise pressure reflex. The regulation and integration of these reflexes operating during dynamic exercise and their possible role in the pathophysiology of some cardiovascular diseases are also discusse

Real-time assembly of ribonucleoprotein complexes on nascent RNA transcripts.

Cellular protein-RNA complexes assemble on nascent transcripts, but methods to observe transcription and protein binding in real time and at physiological concentrations are not available. Here, we report a single-molecule approach based on zero-mode waveguides that simultaneously tracks transcription progress and the binding of ribosomal protein S15 to nascent RNA transcripts during early ribosome biogenesis. We observe stable binding of S15 to single RNAs immediately after transcription for the majority of the transcripts at 35 °C but for less than half at 20 °C. The remaining transcripts exhibit either rapid and transient binding or are unable to bind S15, likely due to RNA misfolding. Our work establishes the foundation for studying transcription and its coupled co-transcriptional processes, including RNA folding, ligand binding, and enzymatic activity such as in coupling of transcription to splicing, ribosome assembly or translation

Hypervelocity Stars from the Andromeda Galaxy

Hypervelocity stars (HVSs) discovered in the Milky Way (MW) halo are thought to be ejected from near the massive black hole (MBH) at the galactic centre. In this paper we investigate the spatial and velocity distributions of the HVSs which are expected to be similarly produced in the Andromeda galaxy (M31). We consider three different HVS production mechanisms: (i) the disruption of stellar binaries by the galactocentric MBH; (ii) the ejection of stars by an in-spiraling intermediate mass black hole; and (iii) the scattering of stars off a cluster of stellar-mass black holes orbiting around the MBH. While the first two mechanisms would produce large numbers of HVSs in M31, we show that the third mechanism would not be effective in M31. We numerically calculate 1.2*10^6 trajectories of HVSs from M31 within a simple model of the Local Group and hence infer the current distribution of these stars. Gravitational focusing of the HVSs by the MW and the diffuse Local Group medium leads to high densities of low mass (~ solar mass) M31 HVSs near the MW. Within the virialized MW halo, we expect there to be of order 1000 HVSs for the first mechanism and a few hundred HVSs for the second mechanism; many of these stars should have distinctively large approach velocities (< -500 km/s). In addition, we predict ~5 hypervelocity RGB stars within the M31 halo which could be identified observationally. Future MW astrometric surveys or searches for distant giants could thus find HVSs from M31.Comment: 14 pages, 6 figures, changed to match version accepted by MNRA

The climate emergency across business, community and campaign groups: motivations and barriers to driving change for the common good

Initiatives to help tackle the climate emergency have tended to focus on large-scale actions that governments can take and smaller-scale actions for individuals, with relatively limited attention given to the mass of groups that form society between these two ends of the spectrum. The aim here is to help close that gap and the chosen areas of study are business, community, and campaign groups. The approach was to assess the existing literature and to conduct three focus groups to identify what motivates these groups to take actions on climate change, while also uncovering the barriers that may drive choices of inaction. The findings indicate that multiple factors motivate environmental engagements across business, community, and campaign groups, but personal ethics and concern for the future play the most vital roles. The most common barriers cited were difficulties in accessing support schemes, a lack of clear accessible information, and financial implications. Better networking and knowledge exchange are considered essential for meaningful progress. This research provides a new framework, upon which many organisations can be better motivated to take actions in helping deal with the global climate emergency facing humanity. Practical action guides may be developed from existing materials, and small taskforces could be trained to provide direct hands-on support to groups across society, especially those in SMEs and local communities. The energies of younger people and campaigners, combined with the experiences of other generations, would create a powerful force for good

Nuclear alpha-clustering, superdeformation, and molecular resonances

Nuclear alpha-clustering has been the subject of intense study since the advent of heavy-ion accelerators. Looking back for more than 40 years we are able today to see the connection between quasimolecular resonances in heavy-ion collisions and extremely deformed states in light nuclei. For example superdeformed bands have been recently discovered in light N=Z nuclei such as $^{36}$Ar, $^{40}$Ca, $^{48}$Cr, and $^{56}$Ni by $\gamma$-ray spectroscopy. The search for strongly deformed shapes in N=Z nuclei is also the domain of charged-particle spectroscopy, and our experimental group at IReS Strasbourg has studied a number of these nuclei with the charged particle multidetector array {\sc Icare} at the {\sc Vivitron} Tandem facility in a systematical manner. Recently the search for $\gamma$-decays in $^{24}$Mg has been undertaken in a range of excitation energies where previously nuclear molecular resonances were found in $^{12}$C+$^{12}$C collisions. The breakup reaction $^{24}$Mg$+^{12}$C has been investigated at E$_{lab}$($^{24}$Mg) = 130 MeV, an energy which corresponds to the appropriate excitation energy in $^{24}$Mg for which the $^{12}$C+$^{12}$C resonance could be related to the breakup resonance. Very exclusive data were collected with the Binary Reaction Spectrometer in coincidence with {\sc Euroball IV} installed at the {\sc Vivitron}.Comment: 10 pages, 4 eps figures included. Invited Talk 10th Nuclear Physics Workshop Marie and Pierre Curie, Kazimierz Dolny Poland, Sep. 24-28, 2003; To be published in International Journal of Modern Physics

Induction and repression of the sty operon in Pseudomonas putida CA-3 during growth on phenylacetic acid under organic and inorganic nutrient-limiting continuous culture conditions

The effects of various nutrient-limiting conditions on expression of the sty operon in Pseudomonas putida CA-3 were investigated. It was observed that limiting concentrations of the carbon source phenylacetic acid, resulted in high levels of phenylacetyl coenzyme A (CoA) ligase activity, this was accompanied also by upper pathway styrene monooxygenase enzyme activity. The introduction of inorganic nutrient limitations, (nitrate, sulfate and phosphate), caused a dramatic reduction in detectable levels of phenylacetyl CoA ligase activity, particularly in the presence of the primary carbon source, succinate. Under these conditions it was no longer possible to detect styrene monooxygenase activity. Reverse transcription PCR analysis of total RNA, isolated under each of the continuous culture conditions examined, revealed that variations in the levels of enzyme activity coincided with altered patterns of corresponding paaK (phenylacetyl CoA ligase) and styA (styrene monooxygenase) gene expression. Transcription of the upper pathway regulatory sensor kinase gene styS was also observed to be growth condition-dependent. These observations suggest that induction/repression of the sty operon in P. putida CA-3, during growth on phenylacetic acid under continuous culture conditions, involves regulatory mechanisms coordinately affecting both the upper and lower pathways and acting at the level of gene transcriptio

Massive open star clusters using the VVV survey III: A young massive cluster at the far edge of the Galactic bar

Context: Young massive clusters are key to map the Milky Way's structure, and near-IR large area sky surveys have contributed strongly to the discovery of new obscured massive stellar clusters. Aims: We present the third article in a series of papers focused on young and massive clusters discovered in the VVV survey. This article is dedicated to the physical characterization of VVV CL086, using part of its OB-stellar population. Methods: We physically characterized the cluster using $JHK_S$ near-infrared photometry from ESO public survey VVV images, using the VVV-SkZ pipeline, and near-infrared $K$-band spectroscopy, following the methodology presented in the first article of the series. Results: Individual distances for two observed stars indicate that the cluster is located at the far edge of the Galactic bar. These stars, which are probable cluster members from the statistically field-star decontaminated CMD, have spectral types between O9 and B0V. According to our analysis, this young cluster ($1.0$ Myr $<$ age $< 5.0$ Myr) is located at a distance of $11^{+5}_{-6}$ kpc, and we estimate a lower limit for the cluster total mass of $(2.8^{+1.6}_{-1.4})\cdot10^3 {M}_{\odot}$. It is likely that the cluster contains even earlier and more massive stars.Comment: Accepted for publication as a Letter in A&