273 research outputs found
Neural Mechanisms Underlying the Generation of the Lobster Gastric Mill Motor Pattern
The lobster gastric mill central pattern generator (CPG) is located in the stomatogastric ganglion and consists of 11 neurons whose circuitry is well known. Because all of the neurons are identifiable and accessible, it can serve as a prime experimental model for analyzing how microcircuits generate multiphase oscillatory spatiotemporal patterns. The neurons that comprise the gastric mill CPG consist of one interneuron, five burster neurons and six tonically firing neurons. The single interneuron (Int 1) is shared by the medial tooth subcircuit (containing the AM, DG and GMs) and the lateral teeth subcircuit (LG, MG and LPGs). By surveying cell-to-cell connections and the cooperative dynamics of the neurons we find that the medial subcircuit is essentially a feed forward system of oscillators. The Int 1 neuron entrains the DG and AM cells by delayed excitation and this pair then periodically inhibits the tonically firing GMs causing them to burst. The lateral subcircuit consists of two negative feedback loops of reciprocal inhibition from Int 1 to the LG/MG pair and from the LG/MG to the LPGs. Following a fast inhibition from Int 1, the LG/MG neurons receive a slowly developing excitatory input similar to that which Int 1 puts onto DG/AM. Thus Int 1 plays a key role in synchronizing both subcircuits. This coordinating role is assisted by additional, weaker connections between the two subsets but those are not sufficient to synchronize them in the absence of Int 1. In addition to the experiments, we developed a conductance-based model of a slightly simplified gastric circuit. The mathematical model can reproduce the fundamental rhythm and many of the experimentally induced perturbations. Our findings shed light on the functional role of every cell and synapse in this small circuit providing a detailed understanding of the rhythm generation and pattern formation in the gastric mill network
Cosmic-ray induced background intercomparison with actively shielded HPGe detectors at underground locations
The main background above 3\,MeV for in-beam nuclear astrophysics studies
with -ray detectors is caused by cosmic-ray induced secondaries. The
two commonly used suppression methods, active and passive shielding, against
this kind of background were formerly considered only as alternatives in
nuclear astrophysics experiments. In this work the study of the effects of
active shielding against cosmic-ray induced events at a medium deep location is
performed. Background spectra were recorded with two actively shielded HPGe
detectors. The experiment was located at 148\,m below the surface of the Earth
in the Reiche Zeche mine in Freiberg, Germany. The results are compared to data
with the same detectors at the Earth's surface, and at depths of 45\,m and
1400\,m, respectively.Comment: Minor errors corrected; final versio
StdpC: a modern dynamic clamp
With the advancement of computer technology many novel uses of dynamic clamp have become possible. We have added new features to our dynamic clamp software StdpC (âSpike timing-dependent plasticity Clampâ) allowing such new applications while conserving the ease of use and installation of the popular earlier Dynclamp 2/4 package. Here, we introduce the new features of a waveform generator, freely programmable HodgkinâHuxley conductances, learning synapses, graphic data displays, and a powerful scripting mechanism and discuss examples of experiments using these features. In the first example we built and âvoltage clampedâ a conductance based model cell from a passive resistorâcapacitor (RC) circuit using the dynamic clamp software to generate the voltage-dependent currents. In the second example we coupled our new spike generator through a burst detection/burst generation mechanism in a phase-dependent way to a neuron in a central pattern generator and dissected the subtle interaction between neurons, which seems to implement an information transfer through intraburst spike patterns. In the third example, making use of the new plasticity mechanism for simulated synapses, we analyzed the effect of spike timing-dependent plasticity (STDP) on synchronization revealing considerable enhancement of the entrainment of a post-synaptic neuron by a periodic spike train. These examples illustrate that with modern dynamic clamp software like StdpC, the dynamic clamp has developed beyond the mere introduction of artificial synapses or ionic conductances into neurons to a universal research tool, which might well become a standard instrument of modern electrophysiology
The Maturation of Interference Suppression and Response Inhibition: ERP Analysis of a Cued Go/Nogo Task
Inhibitory control is a core function that allows us to resist interference from our surroundings and to stop an ongoing action. To date, it is not clear whether inhibitory control is a single process or whether it is composed of different processes. Further, whether these processes are separate or clustered in childhood is under debate. In this study, we investigated the existence and development of two hypothesized component processes of inhibitory control-interference suppression and response inhibition-using a single task and event related potential components. Twenty 8-year-old children and seventeen adults performed a spatially cued Go/Nogo task while their brain activity was recorded using electroencephalography. Mean N2 amplitudes confirmed the expected pattern for response inhibition with both the children and the adults showing more negative N2 for Nogo vs. Go trials. The interference suppression N2 effect was only present in adults and appeared as a more negative N2 in response to Go trials with a congruent cue than Go trials with an incongruent cue. Contrary to previous findings, there was no evidence that the interference suppression N2 effect was later occurring than the response inhibition N2 effect. Overall, response inhibition was present in both the children and the adults whereas interference suppression was only present in the adults. These results provide evidence of distinct maturational processes for both component processes of inhibitory control, with interference suppression probably continuing to develop into late childhood.This study was supported by a PhD scholarship received by LV from the Faculty of Education at the University of Cambridge, UK
Determination of gamma-ray widths in N using nuclear resonance fluorescence
The stable nucleus N is the mirror of O, the bottleneck in the
hydrogen burning CNO cycle. Most of the N level widths below the proton
emission threshold are known from just one nuclear resonance fluorescence (NRF)
measurement, with limited precision in some cases. A recent experiment with the
AGATA demonstrator array determined level lifetimes using the Doppler Shift
Attenuation Method (DSAM) in O. As a reference and for testing the
method, level lifetimes in N have also been determined in the same
experiment. The latest compilation of N level properties dates back to
1991. The limited precision in some cases in the compilation calls for a new
measurement in order to enable a comparison to the AGATA demonstrator data. The
widths of several N levels have been studied with the NRF method. The
solid nitrogen compounds enriched in N have been irradiated with
bremsstrahlung. The -rays following the deexcitation of the excited
nuclear levels were detected with four HPGe detectors. Integrated
photon-scattering cross sections of ten levels below the proton emission
threshold have been measured. Partial gamma-ray widths of ground-state
transitions were deduced and compared to the literature. The photon scattering
cross sections of two levels above the proton emission threshold, but still
below other particle emission energies have also been measured, and proton
resonance strengths and proton widths were deduced. Gamma and proton widths
consistent with the literature values were obtained, but with greatly improved
precision.Comment: Final published version, minor grammar changes, 10 pages, 4 figures,
8 tables; An addendum is published where the last section is revised: T.
Sz\"ucs and P. Mohr, Phys. Rev. C 92, 044328 (2015) [arXiv:1510.04956
Progress of the Felsenkeller shallow-underground accelerator for nuclear astrophysics
Low-background experiments with stable ion beams are an important tool for
putting the model of stellar hydrogen, helium, and carbon burning on a solid
experimental foundation. The pioneering work in this regard has been done by
the LUNA collaboration at Gran Sasso, using a 0.4 MV accelerator. In the
present contribution, the status of the project for a higher-energy underground
accelerator is reviewed. Two tunnels of the Felsenkeller underground site in
Dresden, Germany, are currently being refurbished for the installation of a 5
MV high-current Pelletron accelerator. Construction work is on schedule and
expected to complete in August 2017. The accelerator will provide intense, 50
uA, beams of 1H+, 4He+, and 12C+ ions, enabling research on astrophysically
relevant nuclear reactions with unprecedented sensitivity.Comment: Submitted to the Proceedings of Nuclei in the Cosmos XIV, 19-24 June
2016, Niigata/Japa
Synchronous Behavior of Two Coupled Electronic Neurons
We report on experimental studies of synchronization phenomena in a pair of
analog electronic neurons (ENs). The ENs were designed to reproduce the
observed membrane voltage oscillations of isolated biological neurons from the
stomatogastric ganglion of the California spiny lobster Panulirus interruptus.
The ENs are simple analog circuits which integrate four dimensional
differential equations representing fast and slow subcellular mechanisms that
produce the characteristic regular/chaotic spiking-bursting behavior of these
cells. In this paper we study their dynamical behavior as we couple them in the
same configurations as we have done for their counterpart biological neurons.
The interconnections we use for these neural oscillators are both direct
electrical connections and excitatory and inhibitory chemical connections: each
realized by analog circuitry and suggested by biological examples. We provide
here quantitative evidence that the ENs and the biological neurons behave
similarly when coupled in the same manner. They each display well defined
bifurcations in their mutual synchronization and regularization. We report
briefly on an experiment on coupled biological neurons and four dimensional ENs
which provides further ground for testing the validity of our numerical and
electronic models of individual neural behavior. Our experiments as a whole
present interesting new examples of regularization and synchronization in
coupled nonlinear oscillators.Comment: 26 pages, 10 figure
Neutron-induced background by an alpha-beam incident on a deuterium gas target and its implications for the study of the 2H(alpha,gamma)6Li reaction at LUNA
The production of the stable isotope Li-6 in standard Big Bang
nucleosynthesis has recently attracted much interest. Recent observations in
metal-poor stars suggest that a cosmological Li-6 plateau may exist. If true,
this plateau would come in addition to the well-known Spite plateau of Li-7
abundances and would point to a predominantly primordial origin of Li-6,
contrary to the results of standard Big Bang nucleosynthesis calculations.
Therefore, the nuclear physics underlying Big Bang Li-6 production must be
revisited. The main production channel for Li-6 in the Big Bang is the
2H(alpha,gamma)6Li reaction. The present work reports on neutron-induced
effects in a high-purity germanium detector that were encountered in a new
study of this reaction. In the experiment, an {\alpha}-beam from the
underground accelerator LUNA in Gran Sasso, Italy, and a windowless deuterium
gas target are used. A low neutron flux is induced by energetic deuterons from
elastic scattering and, subsequently, the 2H(d,n)3He reaction. Due to the
ultra-low laboratory neutron background at LUNA, the effect of this weak flux
of 2-3 MeV neutrons on well-shielded high-purity germanium detectors has been
studied in detail. Data have been taken at 280 and 400 keV alpha-beam energy
and for comparison also using an americium-beryllium neutron source.Comment: Submitted to EPJA; 13 pages, 8 figure
First Direct Measurement of the ^{17}O(p,\gamma)^{18}F Reaction Cross-Section at Gamow Energies for Classical Novae
Classical novae are important contributors to the abundances of key isotopes,
such as the radioactive ^{18}F, whose observation by satellite missions could
provide constraints on nucleosynthesis models in novae. The
^{17}O(p,\gamma)^{18}F reaction plays a critical role in the synthesis of both
oxygen and fluorine isotopes but its reaction rate is not well determined
because of the lack of experimental data at energies relevant to novae
explosions. In this study, the reaction cross section has been measured
directly for the first time in a wide energy range Ecm = 200 - 370 keV
appropriate to hydrogen burning in classical novae. In addition, the E=183 keV
resonance strength, \omega \gamma=1.67\pm0.12 \mueV, has been measured with the
highest precision to date. The uncertainty on the ^{17}O(p,\gamma)^{18}F
reaction rate has been reduced by a factor of 4, thus leading to firmer
constraints on accurate models of novae nucleosynthesis.Comment: accepted by Phys. Rev. Let
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