384 research outputs found
Critical Decay at Higher-Order Glass-Transition Singularities
Within the mode-coupling theory for the evolution of structural relaxation in
glass-forming systems, it is shown that the correlation functions for density
fluctuations for states at A_3- and A_4-glass-transition singularities can be
presented as an asymptotic series in increasing inverse powers of the logarithm
of the time t: , where
with p_n denoting some polynomial and x=ln (t/t_0). The results are
demonstrated for schematic models describing the system by solely one or two
correlators and also for a colloid model with a square-well-interaction
potential.Comment: 26 pages, 7 figures, Proceedings of "Structural Arrest Transitions in
Colloidal Systems with Short-Range Attractions", Messina, Italy, December
2003 (submitted
Mitochondrial DNA mutations in renal cell carcinomas revealed no general impact on energy metabolism
Previously, renal cell carcinoma tissues were reported to display a marked reduction of components of the respiratory chain. To elucidate a possible relationship between tumourigenesis and alterations of oxidative phosphorylation, we screened for mutations of the mitochondrial DNA (mtDNA) in renal carcinoma tissues and patient-matched normal kidney cortex. Seven of the 15 samples investigated revealed at least one somatic heteroplasmic mutation as determined by denaturating HPLC analysis (DHPLC). No homoplasmic somatic mutations were observed. Actually, half of the mutations presented a level of heteroplasmy below 25%, which could be easily overlooked by automated sequence analysis. The somatic mutations included four known D-loop mutations, four so far unreported mutations in ribosomal genes, one synonymous change in the ND4 gene and four nonsynonymous base changes in the ND2, COI, ND5 and ND4L genes. One renal cell carcinoma tissue showed a somatic A3243G mutation, which is a known frequent cause of MELAS syndrome (mitochondrial encephalomyopathy, lactic acidosis, stroke-like episode) and specific compensatory alterations of enzyme activities of the respiratory chain in the tumour tissue. No difference between histopathology and clinical progression compared to the other tumour tissues was observed. In conclusion, the low abundance as well as the frequently observed low level of heteroplasmy of somatic mtDNA mutations indicates that the decreased aerobic energy capacity in tumour tissue seems to be mediated by a general nuclear regulated mechanism
Multiplex primer extension analysis for rapid detection of major European mitochondrial haplogroups
The evolution of the human mitochondrial genome is reflected in the existence of eth- nically distinct lineages or haplogroups. Alterations of mitochondrial DNA (mtDNA) have been instrumental in studies of human phylogeny, in population genetics, and in molecular medicine to link pathological mutations to a variety of human diseases of complex etiology. For each of these applications, rapid and cost effective assays for mtDNA haplogrouping are invaluable. Here we describe a hierarchical system for mtDNA haplogrouping that combines multiplex PCR amplifications, multiplex single- base primer extensions, and CE for analyzing ten haplogroup-diagnostic mitochondrial single nucleotide polymorphisms. Using this rapid and cost-effective mtDNA geno- typing method, we were able to show that within a large, randomly selected cohort of healthy Austrians ( n = 1172), mtDNAs could be assigned to all nine major European haplogroups. Forty-four percent belonged to haplogroup H, the most frequent hap- logroup in European Caucasian populations. The other major haplogroups identified were U (15.4%), J (11.8%), T (8.2%) and K (5.1%). The frequencies of haplogroups in Austria is within the range observed for other European countries. Our method may be suitable for mitochondrial genotyping of samples from large-scale epidemiology stud- ies and for identifying markers of genetic susceptibility
Unoccupied states of individual silver clusters and chains on Ag(111)
Size-selected silver clusters on Ag(111) were fabricated with the tip of a
scanning tunneling microscope. Unoccupied electron resonances give rise to
image contrast and spectral features which shift toward the Fermi level with
increasing cluster size. Linear assemblies exhibit higher resonance energies
than equally sized compact assemblies. Density functional theory calculations
reproduce the observed energies and enable an assignment of the resonances to
hybridized atomic 5s and 5p orbitals with silver substrate states.Comment: 9 pages, 8 figure
Severe depletion of mitochondrial DNA in spinal muscular atrophy
Spinal muscular atrophy (SMA) is a neuromus- cular disorder in childhood leading to a dramatic loss of muscle strength. Functional investigations with high-reso- lution polarography and enzyme measurements of the res- piratory chain revealed lowered activities in muscle tissue of SMA patients. To gain a better understanding of this low energy supply we analyzed the amount of mitochon- drial DNA (mtDNA) in skeletal muscle of 20 unrelated children with genetically proven SMA and 31 controls. Quantitative Southern blot analysis revealed a severe and homogeneous decrease in the content of muscle mtDNA in relation to nuclear DNA in SMA patients (90.3±7.8%), whereas by immunofluorescence no decrease in the num- ber of mitochondria was detected. In addition, a two- to threefold reduction of the nuclear-encoded complex II (succinate dehydrogenase) activity was detected in SMA muscle tissue. Western blot analysis showed a significant reduction of both mitochondrial- and nuclear-encoded cy- tochrome c oxidase subunits. Our results indicate that mtDNA depletion in SMA is a consequence of severe at- rophy, and has to be differentiated by measurement of complex II from an isolated reduction of mtDNA as found in patients with mitochondriocytopathies and the so- called mtDNA depletion syndrome
Detection of Solar-like Oscillations in the G7 Giant Star xi Hya
We report the firm discovery of solar-like oscillations in a giant star. We
monitored the star xi Hya (G7III) continuously during one month with the
CORALIE spectrograph attached to the 1.2m Swiss Euler telescope. The 433
high-precision radial-velocity measurements clearly reveal multiple oscillation
frequencies in the range 50 - 130 uHz, corresponding to periods between 2.0 and
5.5 hours. The amplitudes of the strongest modes are slightly smaller than 2
m/s. Current model calculations are compatible with the detected modes.Comment: 4 pages, 4 figures, accepted for publication as a letter in A&
A search for solar-like oscillations in K giants in the globular cluster M4
To expand the range in the colour-magnitude diagram where asteroseismology
can be applied, we organized a photometry campaign to find evidence for
solar-like oscillations in giant stars in the globular cluster M4. The aim was
to detect the comb-like p-mode structure characteristic for solar-like
oscillations in the amplitude spectra. The two dozen main target stars are in
the region of the bump stars and have luminosities in the range 50-140 Lsun. We
collected 6160 CCD frames and light curves for about 14000 stars were
extracted. We obtain high quality light curves for the K giants, but no clear
oscillation signal is detected. High precision differential photometry is
possible even in very crowded regions like the core of M4. Solar-like
oscillations are probably present in K giants, but the amplitudes are lower
than classical scaling laws predict.Comment: 14 pages, 16 figures, accepted for publication in A&
- âŠ