Biological synthesis of fluorescent nanoparticles by cadmium and tellurite resistant Antarctic bacteria: exploring novel natural nanofactories

Indexación: Web of ScienceBackground: Fluorescent nanoparticles or quantum dots (QDs) have been intensely studied for basic and applied research due to their unique size-dependent properties. There is an increasing interest in developing ecofriendly methods to synthesize these nanoparticles since they improve biocompatibility and avoid the generation of toxic byproducts. The use of biological systems, particularly prokaryotes, has emerged as a promising alternative. Recent studies indicate that QDs biosynthesis is related to factors such as cellular redox status and antioxidant defenses. Based on this, the mixture of extreme conditions of Antarctica would allow the development of natural QDs producing bacteria. Results: In this study we isolated and characterized cadmium and tellurite resistant Antarctic bacteria capable of synthesizing CdS and CdTe QDs when exposed to these oxidizing heavy metals. A time dependent change in fluorescence emission color, moving from green to red, was determined on bacterial cells exposed to metals. Biosynthesis was observed in cells grown at different temperatures and high metal concentrations. Electron microscopy analysis of treated cells revealed nanometric electron-dense elements and structures resembling membrane vesicles mostly associated to periplasmic space. Purified biosynthesized QDs displayed broad absorption and emission spectra characteristic of biogenic Cd nanoparticles. Conclusions: Our work presents a novel and simple biological approach to produce QDs at room temperature by using heavy metal resistant Antarctic bacteria, highlighting the unique properties of these microorganisms as potent natural producers of nano-scale materials and promising candidates for bioremediation purposes.http://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-016-0477-

Energy Distribution in disordered elastic Networks

Disordered networks are found in many natural and artificial materials, from gels or cytoskeletal structures to metallic foams or bones. Here, the energy distribution in this type of networks is modeled, taking into account the orientation of the struts. A correlation between the orientation and the energy per unit volume is found and described as a function of the connectivity in the network and the relative bending stiffness of the struts. If one or both parameters have relatively large values, the struts aligned in the loading direction present the highest values of energy. On the contrary, if these have relatively small values, the highest values of energy can be reached in the struts oriented transversally. This result allows explaining in a simple way remodeling processes in biological materials, for example, the remodeling of trabecular bone and the reorganization in the cytoskeleton. Additionally, the correlation between the orientation, the affinity, and the bending-stretching ratio in the network is discussed

On the stability analysis of periodic sine-Gordon traveling waves

We study the spectral stability properties of periodic traveling waves in the sine-Gordon equation, including waves of both subluminal and superluminal propagation velocities as well as waves of both librational and rotational types. We prove that only subluminal rotational waves are spectrally stable and establish exponential instability in the other three cases. Our proof corrects a frequently cited one given by Scott.Comment: 22 pages, 6 figure

Excitability in a nonlinear magnetoacoustic resonator

We report a nonlinear acoustic system displaying excitability. The considered system is a magnetostrictive material where acoustic waves are parametrically generated. For a set of parameters, the system presents homoclinic and heteroclinic dynamics, whose boundaries define a excitability domain. The excitable behaviour is characterized by analyzing the response of the system to different external stimuli. Single spiking and bursting regimes have been identified.Comment: 4 pages, 5 figure

Temporal evolution of the Evershed flow in sunspots. II. Physical properties and nature of Evershed clouds

Context: Evershed clouds (ECs) represent the most conspicuous variation of the Evershed flow in sunspot penumbrae. Aims: We determine the physical properties of ECs from high spatial and temporal resolution spectropolarimetric measurements. Methods: The Stokes profiles of four visible and three infrared spectral lines are subject to inversions based on simple one-component models as well as more sophisticated realizations of penumbral flux tubes embedded in a static ambient field (uncombed models). Results: According to the one-component inversions, the EC phenomenon can be understood as a perturbation of the magnetic and dynamic configuration of the penumbral filaments along which these structures move. The uncombed inversions, on the other hand, suggest that ECs are the result of enhancements in the visibility of penumbral flux tubes. We conjecture that the enhancements are caused by a perturbation of the thermodynamic properties of the tubes, rather than by changes in the vector magnetic field. The feasibility of this mechanism is investigated performing numerical experiments of thick penumbral tubes in mechanical equilibrium with a background field. Conclusions: While the one-component inversions confirm many of the properties indicated by a simple line parameter analysis (Paper I of this series), we tend to give more credit to the results of the uncombed inversions because they take into account, at least in an approximate manner, the fine structure of the penumbra.Comment: Accepted for publication in A&

Plasmon enhanced energy-transfer up-conversion in Yb3+-Er3+ co-doped LiNbO3 crystal

We have analyzed the effect of linear chains of metallic Ag nanoparticles on the optical properties of a periodically poled Yb 3+ -Er 3+ co-doped LiNbO 3 crystal. By exploiting the broad plasmonic response supported by linear chains of strongly coupled Ag nanoparticles, we demonstrate a 50% of enhancement of the up-converted Er 3+ emission under excitation in the f-f transition of Yb 3+ ions. The observed intensification is explained in terms of the broad plasmonic spectral response supported by the Ag chains, which overlaps both the Er 3+ visible emissions and the Yb 3+ absorption band, and the two-photon character of the Yb 3+ → Er 3+ energy-transfer up-conversion process. The results are of interest for applications involving luminescence up-conversion such as sensing, solar energy conversion, biological imaging or solid-state nanolasersThis work has been supported by the Spanish Ministry of Economy and Competitiveness (MINECO) under project MAT2013- 43301-R and Comunidad de Madrid under grant S2013/MIT-274

CRISP Spectropolarimetric Imaging of Penumbral Fine Structure

We discuss penumbral fine structure in a small part of a pore, observed with the CRISP imaging spectropolarimeter at the Swedish 1-m Solar Telescope (SST), close to its diffraction limit of 0.16 arcsec. Milne-Eddington inversions applied to these Stokes data reveal large variations of field strength and inclination angle over dark-cored penumbral intrusions and a dark-cored light bridge. The mid-outer part of this penumbra structure shows 0.3 arcsec wide spines, separated by 1.6 arcsec (1200 km) and associated with 30 deg inclination variations. Between these spines, there are no small-scale magnetic structures that easily can be be identified with individual flux tubes. A structure with nearly 10 deg more vertical and weaker magnetic field is seen midways between two spines. This structure is co-spatial with the brightest penumbral filament, possibly indicating the location of a convective upflow from below.Comment: Accepted for publication in ApJL 17 Oct 2008. One Figure adde

Resolving the Azimuthal Ambiguity in Vector Magnetogram Data with the Divergence-Free Condition: Application to Discrete Data

We investigate how the divergence-free property of magnetic fields can be exploited to resolve the azimuthal ambiguity present in solar vector magnetogram data, by using line-of-sight and horizontal heliographic derivative information as approximated from discrete measurements. Using synthetic data we test several methods that each make different assumptions about how the divergence-free property can be used to resolve the ambiguity. We find that the most robust algorithm involves the minimisation of the absolute value of the divergence summed over the entire field of view. Away from disk centre this method requires the sign and magnitude of the line-of-sight derivatives of all three components of the magnetic field vector.Comment: Solar Physics, in press, 20 pages, 11 figure