223 research outputs found

    A classification scheme for annotating speech acts in a business email corpus

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    This paper reports on the process of manual annotation of speech acts in a corpus of business emails, in the context of the PROBE project (PRagmatics of Business English). The project aims to bring together corpus, computational, and theoretical linguistics by drawing on the insights made available by the annotated corpus. The corpus data sheds light on the linguistic and discourse structures of speech act use in business email communication. This enhanced linguistic description can be compared to theoretical linguistic representations of speech act categories to assess how well traditional distinctions relate to real-world, naturally occurring data. From a computational perspective, the annotated data is required for the development of an automated speech act tagging tool. Central to this research is the creation of a high quality, manually annotated speech act corpus, using an easily interpretable classification scheme. We discuss the scheme chosen for the project and the training guidelines given to the annotators, and describe the main challenges identified by the annotators

    Negotiating stance within discourses of class: reactions to Benefits Street

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    In this article, we examine the way that audiences respond to particular representations of poverty. Using clips from the Channel 4 television programme Benefits Street we conducted focus groups in four locations across the UK, working with people from different socioeconomic backgrounds who had different experiences with the benefits system. Benefits Street (2014) is an example of reality television where members of the public are followed by film crews as they perform everyday tasks and routines. Our choice to focus on this particular programme was prompted by the huge media response that it received when it was broadcast; Benefits Street generated 950 complaints to regulatory watchdog Ofcom (2014) and was referred to as ‘poverty porn’ (Clark, 2014). We focus on the way that viewers of this programme produce assessments of those on benefits, analysing the discursive strategies used by our participants when evaluating representations of those on benefits. Specifically, we consider how the participants in our study construct their own stance and attribute stance to others through naming and agency practices, the negotiation of opinion, and stake inoculation. We invited our participants to judge the people they saw on screen, but they went beyond this. They used clips of the programme as stimuli to collaboratively construct an overarchingly-negative stereotype of those on benefits. We conclude that Benefits Street is not just an entertainment programme, but is rather a site for ideological construction and the perpetuation of existing stereotypes about benefit claimants. The programme (and others like it) invites negative evaluations of those on benefits and is thus a worthy site for critical linguistic analysis

    Re-reading in Stylistics

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    Cognitive stylistics is primarily concerned with the cognitive processes – mental simulations – experienced by readers. Most cognitive stylisticians agree that experiences of reading texts are dynamic and flexible. Changes in the context of reading, our attentional focus on a given day, our extra background knowledge about the text, and so on, are all factors that contribute to our experience of a fictional world. A second reading of a text is a different experience to a first reading. As researchers begin to systematically distinguish between the ‘solitary’ and ‘social’ readings that constitute reading as a phenomenon (Peplow et al., 2016), the relationship between multiple readings and the nature of their processing become increasingly pertinent. In order to explore this relationship, firstly we examine the different ways in which re-reading has previously been discussed in stylistics, grounding our claims in an empirical analysis of articles published in key stylistics journals over the past two decades. Next, we draw on reader response data from an online questionnaire in order to assess the role of re-reading and the motivations that underpin it. Finally, we describe an exercise for the teaching of cognitive stylistics, specifically applying schema theory in literary linguistic analysis (Cook, 1994), which illustrates the need to distinguish between readings as part of an analysis. Through these three sections we argue that our experiences of texts should be considered diachronically, and propose that the different readings that make up an analysis of a text should be given greater attention in stylistic research and teaching

    Genetic Traces of Recent Long-Distance Dispersal in a Predominantly Self-Recruiting Coral

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    Understanding of the magnitude and direction of the exchange of individuals among geographically separated subpopulations that comprise a metapopulation (connectivity) can lead to an improved ability to forecast how fast coral reef organisms are likely to recover from disturbance events that cause extensive mortality. Reef corals that brood their larvae internally and release mature larvae are believed to show little exchange of larvae over ecological times scales and are therefore expected to recover extremely slowly from large-scale perturbations.Using analysis of ten DNA microsatellite loci, we show that although Great Barrier Reef (GBR) populations of the brooding coral, Seriatopora hystrix, are mostly self-seeded and some populations are highly isolated, a considerable amount of sexual larvae (up to approximately 4%) has been exchanged among several reefs 10 s to 100 s km apart over the past few generations. Our results further indicate that S. hystrix is capable of producing asexual propagules with similar long-distance dispersal abilities (approximately 1.4% of the sampled colonies had a multilocus genotype that also occurred at another sampling location), which may aid in recovery from environmental disturbances.Patterns of connectivity in this and probably other GBR corals are complex and need to be resolved in greater detail through genetic characterisation of different cohorts and linkage of genetic data with fine-scale hydrodynamic models

    Identification of Pathogenicity-Related Genes in the Vascular Wilt Fungus Verticillium dahliae by Agrobacterium tumefaciens-Mediated T-DNA Insertional Mutagenesis

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    Verticillium dahliae is the causal agent of vascular wilt in many economically important crops worldwide. Identification of genes that control pathogenicity or virulence may suggest targets for alternative control methods for this fungus. In this study, Agrobacteriumtumefaciens-mediated transformation (ATMT) was applied for insertional mutagenesis of V. dahliae conidia. Southern blot analysis indicated that T-DNAs were inserted randomly into the V. dahliae genome and that 69% of the transformants were the result of single copy T-DNA insertion. DNA sequences flanking T-DNA insertion were isolated through inverse PCR (iPCR), and these sequences were aligned to the genome sequence to identify the genomic position of insertion. V. dahliae mutants of particular interest selected based on culture phenotypes included those that had lost the ability to form microsclerotia and subsequently used for virulence assay. Based on the virulence assay of 181 transformants, we identified several mutant strains of V. dahliae that did not cause symptoms on lettuce plants. Among these mutants, T-DNA was inserted in genes encoding an endoglucanase 1 (VdEg-1), a hydroxyl-methyl glutaryl-CoA synthase (VdHMGS), a major facilitator superfamily 1 (VdMFS1), and a glycosylphosphatidylinositol (GPI) mannosyltransferase 3 (VdGPIM3). These results suggest that ATMT can effectively be used to identify genes associated with pathogenicity and other functions in V. dahliae

    Indicadores das condições nutricionais na região Polonoroeste: V. Desnutrição protéico-energética e parasitoses intestinais em um grupo de crianças de 3 a 72 meses de idade da cidade de Mirassol D'Oeste, Mato Grosso, Brasil

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    Este trabalho tem por objetivo caracterizar a desnutrição protéico-energética associada a parasitose intestinal em grupo de 149 crianças de ambos os sexos, na faixa etária de 3 a 72 meses, da cidade de Mirassol D'Oeste, na região do Projeto Polonoroeste em Mato Grosso. De cada criança foram coletados os seguintes dados: sexo, peso, idade e amostra de fezes para exame parasitológico. Os dados peso/idade obtidos foram analisados pelos critérios de GOMEZ. Utilizou-se como padrão de referência o National Center for Health Statistic (NCHS). Para diagnóstico dos parasitas intestinais executou-se o método de Hoffman, Pons e Janer. O grupo estudado constitui-se em sua maioria de crianças desnutridas, sendo a forma leve de desnutrição mais comum que as formas moderada e grave. As enteroparasitoses foram encontradas em 69% das amostras examinadas. A "Giardia lamblia" foi o protozoário mais comum e o "Ancilostomídeo" o helminto mais encontrado. O teste X² não mostrou relação de dependência entre o estado nutricional e a freqüência de enteroparasitoses
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