Potential Impact of Mediterranean Aquaculture on the Wild Predatory Bluefish

Aquaculture impacts on wild populations of fish have been considered principally due to farm escapes. The Bluefish Pomatomus saltatrix, which exhibits two distinct genetic units in the Mediterranean Sea, is a voracious predator and is attracted to aquaculture cages to prey on farmed fish, particularly Gilthead Seabream Sparus aurata and European Sea Bass Dicentrarchus labrax. We compared the genetic diversity of adult Bluefish caught inside one aquaculture farm located in Spanish waters of the western Mediterranean Sea with reference individuals of East and West Mediterranean stocks from the open sea. Bluefish were genetically assigned to their putative origin using seven microsatellite loci and mitochondrial cytochrome oxidase subunit I as molecular markers. As expected, most of the individuals caught from inside the fish farm cages were assigned to the local genetic population. However, between 7.14% and 11.9% of individuals were assigned to the distant and different genetic unit inhabiting Turkish waters, the East Mediterranean stock. The genetic membership of those individuals revealed some degree of interbreeding between the East and West Mediterranean Bluefish stocks. All results suggest that aquaculture acts as an attractor for Bluefish and could affect genetic diversity as well as phylogeography of this fish and maybe other similar species that aggregate around marine fish farms.We are very grateful to T. Ceyhan for providing the Bluefish samples from Turkey. The study was supported by the MICINN CGL-2009-08279 Grant (Spain) and the Asturian Grant GRUPIN2014-093. Laura Miralles held a PCTI Grant from the Asturias Regional Government, referenced BP 10-004. This is a contribution from the Marine Observatory of Asturias

Environmental variables, habitat discontinuity and life history shaping the genetic structure of Pomatoschistus marmoratus

Coastal lagoons are semi-isolated ecosystems exposed to wide fluctuations of environmental conditions and showing habitat fragmentation. These features may play an important role in separating species into different populations, even at small spatial scales. In this study, we evaluate the concordance between mitochondrial (previous published data) and nuclear data analyzing the genetic variability of Pomatoschistus marmoratus in five localities, inside and outside the Mar Menor coastal lagoon (SE Spain) using eight microsatellites. High genetic diversity and similar levels of allele richness were observed across all loci and localities, although significant genic and genotypic differentiation was found between populations inside and outside the lagoon. In contrast to the FST values obtained from previous mitochondrial DNA analyses (control region), the microsatellite data exhibited significant differentiation among samples inside the Mar Menor and between lagoonal and marine samples. This pattern was corroborated using Cavalli-Sforza genetic distances. The habitat fragmentation inside the coastal lagoon and among lagoon and marine localities could be acting as a barrier to gene flow and contributing to the observed genetic structure. Our results from generalized additive models point a significant link between extreme lagoonal environmental conditions (mainly maximum salinity) and P. marmoratus genetic composition. Thereby, these environmental features could be also acting on genetic structure of coastal lagoon populations of P. marmoratus favoring their genetic divergence. The mating strategy of P. marmoratus could be also influencing our results obtained from mitochondrial and nuclear DNA. Therefore, a special consideration must be done in the selection of the DNA markers depending on the reproductive strategy of the species

Most Northerly Observation of a Grizzly Bear (Ursus arctos) in Canada: Photographic and DNA Evidence from Melville Island, Northwest Territories

During geological studies in 2003 and 2004 on Melville Island, Northwest Territories, Canada, field parties photographed and gathered genetic information on one or more grizzly bears (Ursus arctos). To our knowledge, these data constitute the most northerly observations made of this species in North America. The DNA of a hair sample collected on Melville Island in 2004 is genetically indistinguishable from DNA collected from a population of grizzly bears around Paulatuk, Northwest Territories, along the northern mainland coast. It is also distinct from the DNA of the Viscount Melville polar bear (Ursus maritimus) population. Our evidence and review suggest that, at a minimum, transient grizzly bears are now regular visitors to the Canadian Arctic Archipelago. More research will be required to understand the significance of these observations, but a small viable population of grizzly bears may now be using areas in or around Melville Island.Dans le cadre d’études géologiques réalisées en 2003 et en 2004 sur l’île Melville, dans les Territoires du Nord-Ouest, au Canada, des chercheurs sur le terrain ont photographié et recueilli de l’information génétique sur un ou plusieurs grizzlys (Ursus arctos). À notre connaissance, il s’agit des données représentant les observations sur cette espèce qui ont été recueillies les plus au nord de l’Amérique du Nord. L’ADN d’un échantillon de poil prélevé sur l’île Melville en 2004 est indifférenciable, du point de vue génétique, de l’ADN prélevé au sein d’une population de grizzlys de la région de Paulatuk, Territoires du Nord-Ouest, le long de la côte nord. Par ailleurs, il est distinct de l’ADN de la population d’ours polaires du Vicomte de Melville (Ursus maritimus). D’après les preuves que nous avons recueillies et notre analyse, à tout le moins, les grizzlys de passage sont maintenant des visiteurs habituels dans l’archipel Arctique canadien. D’autres recherches devront être effectuées afin de comprendre l’importance de ces observations, mais une population petite, bien que viable, de grizzlys pourrait maintenant utiliser les régions de l’île Melville ou situées tout près

Mitigating Scoring Errors in Microsatellite Data from Wild Populations

Microsatellite data are widely used to test ecological and evolutionary hypotheses in wild populations. In this paper, we consider three typical sources of scoring errors capable of biasing biological conclusions: stuttering, large-allele dropout and null alleles. We describe methods to detect errors and propose conventions to mitigate scoring errors and report error rates in studies of wild populations. Finally, we discuss potential bias in ecological or evolutionary conclusions based on data sets containing these scoring errors

Recent Hybridization between a Polar Bear and Grizzly Bears in the Canadian Arctic

Grizzly bears have recently become more common on the Arctic Islands in the Inuvialuit Settlement Region, concurrently with a period of environmental change. Over the last decade, grizzly bear – polar bear hybrids have been confirmed within this region, triggering extensive discussion and speculation regarding the impact of hybridization on the parent species. Through harvests, sightings, and captures, we document an increase in the presence of grizzly bears and combine field observations of hybrids with genetic analysis and parentage analysis to identify four first-generation (F1) hybrids and four offspring of F1 hybrids and grizzly bears (backcross-to-grizzly-bear individuals). We trace these eight hybrid individuals to a single female polar bear who mated with two grizzly bears. We sampled one of her mates on the sea ice in the High Arctic and deduced the genotype of the other from his five offspring. The two male grizzly bears are sires of both the F1 generation and the backcross-to-grizzly-bear generation. So what initially appeared to be a sudden spate of hybridization in the western Canadian Arctic originated with the unusual mating between three non-hybrid parents. The breakdown of species barriers may start with atypical mating preferences of select individuals; however, the story we present can be traced to a single female polar bear who, along with three of her known F1 offspring, has been killed.La présence du grizzli se fait plus courante dans l’archipel Arctique de la région désignée des Inuvialuit depuis un certain temps, ce qui coïncide avec une période de changement environnemental. Ces dix dernières années, la présence d’ours polaires-grizzlis hybrides a été confirmée dans cette région, ce qui a déclenché d’importantes discussions et hypothèses relativement aux incidences de l’hybridation sur les espèces apparentées. Au moyen de récoltes, d’observations et de captures, nous avons documenté l’augmentation de la présence de grizzlis, alliées à des observations d’hybrides sur le terrain avec analyse génétique et analyse de parenté afin d’identifier quatre hybrides de première génération (F1) et quatre descendants d’hybrides F1 et de grizzlis (individus issus de rétrocroisements avec un grizzli). Nous faisons remonter ces huit hybrides à une seule ourse polaire qui s’est accouplée avec deux grizzlis. Nous avons prélevé un échantillon d’un de ses compagnons sur la glace de mer de l’Extrême-Arctique et avons déduit le génotype de l’autre à partir de ses cinq descendants. Les deux grizzlis mâles sont des géniteurs de la génération F1 et de la génération du rétrocroisement avec un grizzli. Donc, ce qui semblait être, au début, une montée soudaine d’hybridation dans l’ouest de l’Arctique canadien est attribuable à l’accouplement inhabituel de trois parents non hybrides. La rupture des obstacles auxquels les espèces font face pourrait commencer par les préférences d’accouplement atypiques de certains individus. Toutefois, la situation dont il est ici question remonte à une seule ourse polaire qui a été tuée, avec trois de ses descendants connus de la génération F1

Genetic and oceanographic tools reveal high population connectivity and diversity in the endangered pen shell Pinna nobilis

For marine meta-populations with source-sink dynamics knowledge about genetic connectivity is important to conserve biodiversity and design marine protected areas (MPAs). We evaluate connectivity of a Mediterranean sessile species, Pinna nobilis. To address a large geographical scale, partial sequences of cytochrome oxidase I (COI, 590 bp) were used to evaluate phylogeographical patterns in the Western Mediterranean, and in the whole basin using overlapping sequences from the literature (243 bp). Additionally, we combined (1) larval trajectories based on oceanographic currents and early life-history traits and (2) 10 highly polymorphic microsatellite loci collected in the Western Mediterranean. COI results provided evidence for high diversity and low inter-population differentiation. Microsatellite genotypes showed increasing genetic differentiation with oceanographic transport time (isolation by oceanographic distance (IBD) set by marine currents). Genetic differentiation was detected between Banyuls and Murcia and between Murcia and Mallorca. However, no genetic break was detected between the Balearic populations and the mainland. Migration rates together with numerical Lagrangian simulations showed that (i) the Ebro Delta is a larval source for the Balearic populations (ii) Alicante is a sink population, accumulating allelic diversity from nearby populations. The inferred connectivity can be applied in the development of MPA networks in the Western Mediterranean.Spanish Ministry of Economy and Competitiveness [CTM2009-07013]; Ramon y Cajal Fellowship [RYC2014-14970]; Conselleria d'Innovacio, Recerca i Turisme of the Balearic Government; Spanish Ministry of Economy, Industry and Competitiveness IFCT [IF/00998/2014]; FCT [SFRH/BPD/63703/2009, SFRH/BPD/107878/2015, EXCL/AAG-GLO/0661/2012]; National Science Foundation [OCE-1419450]; Albert II of Monaco Foundationinfo:eu-repo/semantics/publishedVersio

On the origin of the invasive olives (Olea europaea L., Oleaceae).

The olive tree (Olea europaea) has successfully invaded several regions in Australia and Pacific islands. Two olive subspecies (subspp. europaea and cuspidata) were first introduced in these areas during the nineteenth century. In the present study, we determine the origin of invasive olives and investigate the importance of historical effects on the genetic diversity of populations. Four invasive populations from Australia and Hawaii were characterized using eight nuclear DNA microsatellites, plastid DNA markers as well as ITS-1 sequences. Based on these data, their genetic similarity with native populations was investigated, and it was determined that East Australian and Hawaiian populations (subsp. cuspidata) have originated from southern Africa while South Australian populations (subsp. europaea) have mostly derived from western or central Mediterranean cultivars. Invasive populations of subsp. cuspidata showed significant loss of genetic diversity in comparison to a putative source population, and a recent bottleneck was evidenced in Hawaii. Conversely, invasive populations of subsp. europaea did not display significant loss of genetic diversity in comparison to a native Mediterranean population. Different histories of invasion were inferred for these two taxa with multiple cultivars introduced restoring gene diversity for europaea and a single successful founder event and sequential introductions to East Australia and then Hawaii for cuspidata. Furthermore, one hybrid (cuspidata x europaea) was identified in East Australia. The importance of hybridizations in the future evolution of the olive invasiveness remains to be investigated

Use of SSR and retrotransposon-based markers to interpret the population structure of native grapevines from Southern Italy

Native grapevines are the quintessential ele- ments of Southern Italy winemaking, and genomic char- acterization plays a role of primary importance for preservation and sustainable use of these unexploited genetic resources. Among the various molecular techniques available, SSR and retrotransposons-based markers result to be the most valuable for cultivars and biotypes distinc- tiveness. A total of 62 accessions including 38 local grape cultivars were analyzed with 30 SSR, four REMAP and one IRAP markers to assess their genetic diversity and obtain a complete genomic profiling. The use of VrZAG79, VrZAG112, VVS2, VVMD25 and VVMD5 combined with retrotransposon-based markers proved to be the most dis- criminating and polymorphic markers for the rapid and unambiguous identification of minority grapevines from Campania region, which is considered one of the most appreciated Italian districts for wine production. Results revealed 58 SSR marker-specific alleles, 22 genotype- specific SSR alleles, and four REMAP and IRAP private bands. Cases of synonymy and homonymy were discov- ered. In conclusion, we provided evidences that the inte- grating SSR and retrotransposon-based markers is an effective strategy to assess the genetic diversity of autochthonous grapes, allowing their easy identification