66 research outputs found
Consistency analysis of metabolic correlation networks
<p>Abstract</p> <p>Background</p> <p>Metabolic correlation networks are derived from the covariance of metabolites in replicates of metabolomics experiments. They constitute an interesting intermediate between topology (i.e. the system's architecture defined by the set of reactions between metabolites) and dynamics (i.e. the metabolic concentrations observed as fluctuations around steady-state values in the metabolic network).</p> <p>Results</p> <p>Here we analyze, how such a correlation network changes over time, and compare the relative positions of metabolites in the correlation networks with those in established metabolic networks derived from genome databases. We find that network similarity indeed decreases with an increasing time difference between these networks during a day/night course and, counter intuitively, that proximity of metabolites in the correlation network is no indicator of proximity of the metabolites in the metabolic network.</p> <p>Conclusion</p> <p>The organizing principles of correlation networks are distinct from those of metabolic reaction maps. Time courses of correlation networks may in the future prove an important data source for understanding these organizing principles.</p
Deployment of contact-based ultrasonic thickness measurements using over-actuated UAVs
Unmanned Aerial Vehicles (UAVs) are increasingly being utilized for the structural health assessment of on and off-shore structures. Visual inspection is the usual methodology for acquiring data from these structures, but there is often a need for contact based structural measurements, for example to assess local thickness on corroding structures. Conventional UAV platform dynamics have not traditionally allowed for such contact measurements. The limited dynamic control afforded by fixed plane rotor UAVs means that forward thrust (to apply contact forces for surface transduction) is only possible by tilting the whole platform, thus taking the UAV into a non-stationary state and limiting positional accuracy. An over-actuated UAV platform (with fully vectored thrust capability) may provide the required contact force for such thickness measurements whilst maintaining stable hovering next to the structure. The authors herein present a contact based ultrasonic thickness measurement technique, whereby an ultrasonic wheel probe deployed from a UAV was used to make single point and scanned measurements across a surface to provide a set of local thickness measurements. A 5 MHz, dry-coupled, dual-element, ultrasonic wheel probe is used to measure the thickness of an aluminum sample plate with thicknesses of 8.2 mm, 4.5 mm and 3.2 mm, and a precision stepped calibration block with size from 31.5 mm to 17.5 mm in steps of 1 mm, then steps of 0.1 mm down to 16.5 mm over a total length of 500 mm. The thickness resolution obtainable from the ultrasonic wheel probe was typically 0.1 mm, and the positional accuracy attained from the over-actuated deployment platform was 16.6 mm when performing single point measurements
A Non-Targeted Approach Unravels the Volatile Network in Peach Fruit
Volatile compounds represent an important part of the plant metabolome and are of particular agronomic and biological interest due to their contribution to fruit aroma and flavor and therefore to fruit quality. By using a non-targeted approach based on HS-SPME-GC-MS, the volatile-compound complement of peach fruit was described. A total of 110 volatile compounds (including alcohols, ketones, aldehydes, esters, lactones, carboxylic acids, phenolics and terpenoids) were identified and quantified in peach fruit samples from different genetic backgrounds, locations, maturity stages and physiological responses. By using a combination of hierarchical cluster analysis and metabolomic correlation network analysis we found that previously known peach fruit volatiles are clustered according to their chemical nature or known biosynthetic pathways. Moreover, novel volatiles that had not yet been described in peach were identified and assigned to co-regulated groups. In addition, our analyses showed that most of the co-regulated groups showed good intergroup correlations that are therefore consistent with the existence of a higher level of regulation orchestrating volatile production under different conditions and/or developmental stages. In addition, this volatile network of interactions provides the ground information for future biochemical studies as well as a useful route map for breeding or biotechnological purposes
Metabolomics Unravel Contrasting Effects of Biodiversity on the Performance of Individual Plant Species
In spite of evidence for positive diversity-productivity relationships increasing plant diversity has highly variable effects on the performance of individual plant species, but the mechanisms behind these differential responses are far from being understood. To gain deeper insights into the physiological responses of individual plant species to increasing plant diversity we performed systematic untargeted metabolite profiling on a number of herbs derived from a grassland biodiversity experiment (Jena Experiment). The Jena Experiment comprises plots of varying species number (1, 2, 4, 8, 16 and 60) and number and composition of functional groups (1 to 4; grasses, legumes, tall herbs, small herbs). In this study the metabolomes of two tall-growing herbs (legume: Medicago x varia; non-legume: Knautia arvensis) and three small-growing herbs (legume: Lotus corniculatus; non-legumes: Bellis perennis, Leontodon autumnalis) in plant communities of increasing diversity were analyzed. For metabolite profiling we combined gas chromatography coupled to time-of-flight mass spectrometry (GC-TOF-MS) and UPLC coupled to FT-ICR-MS (LC-FT-MS) analyses from the same sample. This resulted in several thousands of detected m/z-features. ANOVA and multivariate statistical analysis revealed 139 significantly changed metabolites (30 by GC-TOF-MS and 109 by LC-FT-MS). The small-statured plants L. autumnalis, B. perennis and L. corniculatus showed metabolic response signatures to increasing plant diversity and species richness in contrast to tall-statured plants. Key-metabolites indicated C- and N-limitation for the non-leguminous small-statured species B. perennis and L. autumnalis, while the metabolic signature of the small-statured legume L. corniculatus indicated facilitation by other legumes. Thus, metabolomic analysis provided evidence for negative effects of resource competition on the investigated small-statured herbs that might mechanistically explain their decreasing performance with increasing plant diversity. In contrast, taller species often becoming dominant in mixed plant communities did not show modified metabolite profiles in response to altered resource availability with increasing plant diversity. Taken together, our study demonstrates that metabolite profiling is a strong diagnostic tool to assess individual metabolic phenotypes in response to plant diversity and ecophysiological adjustment
Unpredictability of metabolism—the key role of metabolomics science in combination with next-generation genome sequencing
Next-generation sequencing provides technologies which sequence whole prokaryotic and eukaryotic genomes in days, perform genome-wide association studies, chromatin immunoprecipitation followed by sequencing and RNA sequencing for transcriptome studies. An exponentially growing volume of sequence data can be anticipated, yet functional interpretation does not keep pace with the amount of data produced. In principle, these data contain all the secrets of living systems, the genotype–phenotype relationship. Firstly, it is possible to derive the structure and connectivity of the metabolic network from the genotype of an organism in the form of the stoichiometric matrix N. This is, however, static information. Strategies for genome-scale measurement, modelling and predicting of dynamic metabolic networks need to be applied. Consequently, metabolomics science—the quantitative measurement of metabolism in conjunction with metabolic modelling—is a key discipline for the functional interpretation of whole genomes and especially for testing the numerical predictions of metabolism based on genome-scale metabolic network models. In this context, a systematic equation is derived based on metabolomics covariance data and the genome-scale stoichiometric matrix which describes the genotype–phenotype relationship
Quantitative metabolomics based on gas chromatography mass spectrometry: status and perspectives
Metabolomics involves the unbiased quantitative and qualitative analysis of the complete set of metabolites present in cells, body fluids and tissues (the metabolome). By analyzing differences between metabolomes using biostatistics (multivariate data analysis; pattern recognition), metabolites relevant to a specific phenotypic characteristic can be identified. However, the reliability of the analytical data is a prerequisite for correct biological interpretation in metabolomics analysis. In this review the challenges in quantitative metabolomics analysis with regards to analytical as well as data preprocessing steps are discussed. Recommendations are given on how to optimize and validate comprehensive silylation-based methods from sample extraction and derivatization up to data preprocessing and how to perform quality control during metabolomics studies. The current state of method validation and data preprocessing methods used in published literature are discussed and a perspective on the future research necessary to obtain accurate quantitative data from comprehensive GC-MS data is provided
Spatial variation in the structure and composition of the herbaceous community in a semiarid region of northeastern Brazil
Lasermaterialbearbeitung mit Industrierobotern
You see a demonstration of a laser material processing equipment with a six-axial industrial robot which puts for different processing tasks a CO2 laser with a high beam quality, a pw-CO2 laser as well as diode lasers at your disposition. A telescopic arm transfers the beam power to the work piece. To compensate the positioning deviations of the robot which are greater than those of Cartesian systems with axial co-ordinates, sensor assisted positioning systems are used for laser beam welding. The off-line programming and the test of the processing programs are realised with a simulation system
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