Application of differential pH technique to the determination of urea in Italian wines

A method for the quantification of urea in wine, based on measuring the change in pH when urease is added to the sample, is presented and compared to the conventional dual enzyme (urease/glutamate dehydrogenase) approach. The method is linear in the range 0-30 mg·l-1 in red, white and “raisin” wines, and the detection limit (0.3 mg·l-1) is lower than for the usual enzymatic method. The differential pH technique presented here gives accurate quantification of urea in wine, being unaffected by the presence of ammonium. The amounts of urea in 195 still and sparkling commercially available wines with designation of geographic origin from the most renowned Italian grape growing areas were quantified. 17.4 % of samples were over the 3 mg·l-1 level suggested by the International Organisation of Vine and Wine for urease treatment to limit the potential risk for ethyl carbamate formation during wine ageing. Yeast strains EC1118 and SP665 can minimise urea content in wine.

Elemental and isotopic profiling: a tool for distinguishing the botanical origin of oenological tannins

Much contemporary evidence underscores the pathophysiological importance of Ca2+ handling by acidic organelles such as lysosomes. Whereas our knowledge of how Ca2+ is released from these acidic Ca2+ stores (the ‘outs’) is advancing, we know relatively little about how Ca2+ uptake is effected (the ‘ins’). Here I highlight new work identifying animal Ca2+/H+ (CAX) exchangers that localize to acidic organelles, mediate Ca2+ uptake and regulate cell migration in vivo. Continued molecular definition of the acidic Ca2+ store toolkit provides new insight into Ca2+-dependent function

Distribution of rare earth elements in Vitis vinifera L. 'Chardonnay' berries

The content of 13 rare earth elements (REEs) in the skin, flesh and seeds of 'Chardonnay' berries harvested at technological ripeness in 2 vineyards in Trentino (Italy) with calcareous soils was analysed using inductively coupled plasma mass spectrometry. The total REE content of the berry was 2.1 and 2.7 ug·kg-1 fresh weight (median value, N = 20) respectively in the 2 vineyards, La, Ce and Nd making up about 80 %. Despite the significantly different amounts of REEs in the berries of the 2 vineyards, the percentage content of each element as compared to the total amount of REEs and the percentage distribution in the 3 parts (skin, flesh and seeds) were similar. Overall, 57 % of REEs were localized in the skin, 40 % in the flesh and 3 % in the seeds (median value, N = 40). Most of the elements prevailed in the skin, while there were negligible amounts in the seeds, where the percentage of Eu was around 20 times higher than in the other parts.

Rapid quantification of 4-ethylphenol in wine using high-performance liquid chromatography with a fluorimetric detector

A rapid method was established for quantifying 4-ethylphenol in wine using HPLC with a detector usually present in wine laboratories. It does not require sample preparation and carries out chromatographic separation in less than 5 min, making control of wine production processes easier. The method is linear up to 2000 μg·l-1 with RSD < 3 % over 20 μg·L-1 and gives a detection limit of 4.0 μg·L-1. It was validated in comparison with the HPLC-coulometric array detector, giving comparable results. Its application to the analysis of 720 DOC and table Italian red wines revealed that the 45 % of them had contents of 4-ethylphenol potentially affecting sensory perception of the aroma.

Subdiffusion of nonlinear waves in quasiperiodic potentials

We study the spatio-temporal evolution of wave packets in one-dimensional quasiperiodic lattices which localize linear waves. Nonlinearity (related to two-body interactions) has destructive effect on localization, as recently observed for interacting atomic condensates [Phys. Rev. Lett. 106, 230403 (2011)]. We extend the analysis of the characteristics of the subdiffusive dynamics to large temporal and spatial scales. Our results for the second moment $m_2$ consistently reveal an asymptotic $m_2 \sim t^{1/3}$ and intermediate $m_2 \sim t^{1/2}$ laws. At variance to purely random systems [Europhys. Lett. 91, 30001 (2010)] the fractal gap structure of the linear wave spectrum strongly favors intermediate self-trapping events. Our findings give a new dimension to the theory of wave packet spreading in localizing environments

Determination of gluconic acid in wine using high pressure liquid chromatography with pulsed amperometric detection

The paper establishes a new HPLC method for measurement of the total content of gluconic acid (GA) in wine using a pulsed amperometric detector equipped with a gold electrode cell and an anion exchange column. A NaOH solution was used at a flow rate of 1.5 ml·min-1 as a mobile phase under isocratic conditions. Analysis took 30 min. The linearity of the method was shown up to 500 mg·l-1, with a LOQ of 2.0 mg·l-1 (10σ). Precision (as RSD%) was under 2.5 % in the usual range of GA concentration in natural wines. Accuracy was checked in comparison with the usual enzymatic method.

Confirming the function of a Final Bronze Age wine processing site in the Nuraghe Genna Maria in Villanovaforru (South Sardinia)

The stone artefact in the hut γ of the NuragheGenna Maria, object of this study, is part of a compound still unpublished today and dated to the Nuragic period. It was found during a 1991 excavation, revealing a situation unchanged since the collapse occurred between the 10th and 9th century B.C., thus preserving the situation at the time of the collapse to this day. The presence of tartaric acid - the marker considered to determinate the presence of wines or products deriving from grapes - has been determined using HPLC-DAD and UHPLC-HQOMS. So the findings under examination, together with the overall evaluation of the archaeological aspects examined, suggests to positively consider the stone artifact as a "laccus" (the latin word for wine presses, still used in the Sardinian language today ) for grape crushing. The internal slope of the floor of the "laccus" allowed the extraction of juice with rapid separation of juice from berry skins. The presence in Sardinia of a large number of "stone wine presses" ("palmenti" in Italian) such as that of the Nuraghe Genna Maria studied in this article, brings a contribution to their dating and confirm the existence of an oenological industry on the island in the Archaic period (9th-10th century B.C.)

Auditive direct in utero observation (Audio): A randomized controlled trial for a prenatal demonstration of fetal hearing

Introduction: The objective of this randomized controlled study was to demonstrate whether acoustic stimulation in utero is associated with fetal reactivity which is documentable by car-diotocography. Materials and methods: A monocentric randomized controlled trial was performed at a single university tertiary hospital between September 2016 and July 2017. This study was registered as a randomized clinical trial on clinicaltrail.gov (registration number NCT04622059). Unselected pregnancies at term of gestation were consecutively recruited for the purpose of this study. After 10 min of normal cardiotocography without accelerations (non-stress-test with a basal frequency between 110 and 150 beats/min, normal variability between 6 and 15 b/min, no accelerations, and no fetal movements), fetuses were randomized at a 1:1 ratio to either of the two groups. Fetuses in group A (n = 105) received acoustic stimulation after 10 min from the beginning of the CTG, whereas fetuses in group B received no stimulation (n = 105). The outcome variables investigated were the lapse of time between the beginning of the CTG and the occurrence of the first accelera-tion, and the lapse of time between the beginning of the CTG and the first fetal movement noticed. Results: The lapse of time between the beginning of the CTG and the occurrence of the first acceleration was significantly shorter in the group with acoustic stimulation compared to the other group (14.87 ± 5.01 vs. 21.90 ± 6.94 min, p-value < 0.001 log-rank test). Similarly, the lapse of time between the beginning of the CTG and the occurrence of the first fetal movement was significantly shorter in group A compared to group B (17.77 ± 7.62 vs. 23.28 ± 7.61 min, p-value < 0.001, log-rank test). Fetal cardiac acceleration and the occurrence of a fetal movement during the first 20 min of the CTG were more frequently recorded in group A compared to group B (respectively, 15% vs. 5% and 20% vs. 8%). Conclusion: This RCT showed an early fetal reaction following auditive stimulus, documentable by cardiotocography. Further research is needed to investigate a possible role of acoustic stimulation in utero for the prenatal diagnosis of congenital hypoacusis

Molecular cloning and biochemical characterization of a Cu,Zn-superoxide dismutase from Scedosporium apiospermum.

A Cu,Zn-superoxide dismutase has been characterized from Scedosporium apiospermum, a fungus which often colonizes the respiratory tract of patients with cystic fibrosis. Enzyme production was stimulated by iron starvation. Purification was achieved from mycelial extract from 7-day-old cultures on Amberlite XAD-16. The purified enzyme presented a relative molecular mass of 16.4 kDa under reducing conditions and was inhibited by potassium cyanide and diethyldithiocarbamate, which are two known inhibitors of Cu,Zn-SODs. Its optimum pH was 7.0 and the enzyme retained full activity after pretreatment at temperatures up to 50 degrees C. Moreover, a 450-bp fragment of the gene encoding the enzyme was amplified by PCR using degenerate primers designed from sequence alignment of four fungal Cu,Zn-SODs. Sequence data from this fragment allowed us to design primers which were used to amplify by walking-PCR the flanking regions of the known fragment. SaSODC gene (890 bp) corresponded to a 154 amino acid polypeptide with a predicted molecular mass of 15.9 kDa. A database search for sequence homology revealed for the deduced amino acid sequence 72 and 83% identity rate with Cu,Zn-SODs from Aspergillus fumigatus and Neurospora crassa, respectively. To our knowledge, this enzyme is the first putative virulence factor of S. apiospermum to be characterized

Evaluation of an optimized enzymatic biosensor for ethanol used in apple storage management with low oxygen stress

Ethanol has been proposed to be one of the target molecules to monitor the dynamic controlled atmosphere (DCA) technique during apple storage, measured in the squeezed juice or in the air of the storage chamber. One of the proposed commercial sensors for ethanol in apple juice is based on amperometry, after a two-step enzyme-based reaction that involves a diaphorase and an alcohol-dehydrogenase. Even though this method has been reported to overestimate ethanol, this difference is fairly fixed and it is industrially used to check the correct application of the treatment and to set the gas composition protocols when the maximum acceptable ethanol is reached. During the 2018 harvest, the ethanol concentration in juices measured with the commercial sensor appeared much higher than those usually reported in precedent years, particularly for the lower concentrations. Laboratory experiments suggested that differences between years could be due to the presence of a secondary enzyme activity present in the commercial diaphorase employed. In order to increase the sensitivity and accuracy, it has been evaluated the performance of the biosensor emploting a further diaphorase. The performances of both sensors were compared with those obtained with a gaschromatophy mass spectrometry approach after head space extraction (HS-GC-MS) in which the mass spectra was acquired in selected-ion monitoring mode. Samples belonging to ‘Red Delicious’ cv. were picked up at different temporary points from industrial storage rooms following the application of low oxygen stress. The new biosensor reduced 97% the mean difference respect to the values obtained with the GC-MS method. The difference between sensors was even clearer for samples with concentrations up to 100 mg/L, that could be used as a discriminating value for the evaluation of the technique success in ‘Red Delicious’ apple juice. The increased sensitivity of the sensor allowed a more accurate monitoring of the DCA at industrial conditions, limiting the risks linked to a false positive on the monitoring during storage