201 research outputs found
Identifying the hydraulic transmissivity of an aquifer with noisy hydraulic head data: a comparison study using three direct inversion methods
The parameters required to set up an hydrological model are often obtained by solving an inverse problem, using indirect or direct solution methods. Indirect methods are widely used, in particular when the state variable (here the hydraulic head h) is known at sparse locations only. Despite their flexibility, when the number of parameters rises, their computational requirements can become a challenging issue, together with problems related to non-uniqueness and instability. On the other side, direct methods have other limitations, including a more exhaustive knowledge of the state variables, but are less demanding in terms of computational resources. In this work three direct inversion methods are compared: the Comparison Model Method (CMM), the Double Constraint Method (DCM) and the Differential System Method (DSM). The three methods are compared on a two-dimensional synthetic aquifer, whose geometry, boundary conditions and h measurements are realistic and extracted from a data set collected for the aquifer of Weiach, north of Switzerland. Sequential-Gaussian simulation (SGS) was used to generate a transmissivity field (T ) for the aquifer, which was then used to obtain the h data required by the three methods. A noise of increasing magnitude was added to h to verify the stability of the methods with respect to noisy input data. For a small noise on the input h, the results obtained with the three methods are comparable. However, for more noisy data, the DCM is more robust, whereas the DSM is very sensitive to the position of the starting point for integration and to the T value assigned there
Assessment of spatio-temporal variability of faecal pollution along coastal waters during and after rainfall events.
More than 80% of wastewaters are discharged into rivers or seas, with a negative impact on
water quality along the coast due to the presence of potential pathogens of faecal origin. Escherichia
coli and enterococci are important indicators to assess, monitor, and predict microbial water quality
in natural ecosystems. During rainfall events, the amount of wastewater delivered to rivers and
coastal systems is increased dramatically. This study implements measures capable of monitoring the
pathways of wastewater discharge to rivers and the transport of faecal bacteria to the coastal area
during and following extreme rainfall events. Spatio-temporal variability of faecal microorganisms
and their relationship with environmental variables and sewage outflow in an area located in the
western Adriatic coast (Fano, Italy) was monitored. The daily monitoring during the rainy events
was carried out for two summer seasons, for a total of five sampling periods. These results highlight
that faecal microbial contaminations were related to rainy events with a high flow of wastewater,
with recovery times for the microbiological indicators varying between 24 and 72 h and influenced by
a dynamic dispersion. The positive correlation between ammonium and faecal bacteria at the Arzilla
River and the consequences in seawater can provide a theoretical basis for controlling ammonium
levels in rivers as a proxy to monitor the potential risk of bathing waters pathogen pollution
The hydroxyl functionality and a rigid proximal N are required for forming a novel non-covalent quinine-heme complex
Quinoline antimalarial drugs bind both monomeric and dimeric forms of free heme, with distinct preferences depending on the chemical environment. Under biological conditions, chloroquine (CQ) appears to prefer to bind to μ-oxo dimeric heme, while quinine (QN) preferentially binds monomer. To further explore this important distinction, we study three newly synthesized and several commercially available QN analogues lacking various functional groups. We find that removal of the QN hydroxyl lowers heme affinity, hemozoin (Hz) inhibition efficiency, and antiplasmodial activity. Elimination of the rigid quinuclidyl ring has similar effects, but elimination of either the vinyl or methoxy group does not. Replacing the quinuclidyl N with a less rigid tertiary aliphatic N only partially restores activity. To further study these trends, we probe drug-heme interactions via NMR studies with both Fe and Zn protoporphyrin IX (FPIX, ZnPIX) for QN, dehydroxyQN (DHQN), dequinuclidylQN (DQQN), and deamino-dequinuclidylQN (DADQQN). Magnetic susceptibility measurements in the presence of FPIX demonstrate that these compounds differentially perturb FPIX monomer-dimer equilibrium. We also isolate the QN-FPIX complex formed under mild aqueous conditions and analyze it by mass spectrometry, as well as fluorescence, vibrational, and solid state NMR spectroscopies. The data elucidate key features of QN pharmacology and allow us to propose a refined model for the preferred binding of QN to monomeric FPIX under biologically relevant conditions. With this model in hand, we also propose how QN, CQ, and amodiaquine (AQ) differ in their ability to inhibit Hz formation
Nutrient removal by floating aquatic macrophytes cultured in anaerobically digested flushed dairy manure wastewater
Genetic Structure of Human A/H1N1 and A/H3N2 Influenza Virus on Corsica Island: Phylogenetic Analysis and Vaccine Strain Match, 2006–2010
Background: The aim of this study was to analyse the genetic patterns of Hemagglutinin (HA) genes of influenza A strains circulating on Corsica Island during the 2006-2009 epidemic seasons and the 2009-2010 pandemic season. [br/]
Methods: Nasopharyngeal samples from 371 patients with influenza-like illness (ILI) were collected by General Practitioners (GPs) of the Sentinelles Network through a randomised selection routine. [br/]
Results: Phylogenetic analysis of HA revealed that A/H3N2 strains circulating on Corsica were closely related to the WHO recommended vaccine strains in each analyzed season (2006-2007 to 2008-2009). Seasonal Corsican influenza A/H1N1 isolated during the 2007-2008 season had drifted towards the A/Brisbane/59/2007 lineage, the A/H1N1 vaccine strain for the 2008-2009 season. The A/H1N1 2009 (A/H1N1pdm) strains isolated on Corsica Island were characterized by the S220T mutation specific to clade 7 isolates. It should be noted that Corsican isolates formed a separate sub-clade of clade 7 as a consequence of the presence of the fixed substitution D222E. The percentages of the perfect match vaccine efficacy, estimated by using the p(epitope) model, against influenza viruses circulating on Corsica Island varied substantially across the four seasons analyzed, and tend to be highest for A/H1N1 compared with A/H3N2 vaccines, suggesting that cross-immunity seems to be stronger for the H1 HA gene. [br/]
Conclusion: The molecular analysis of the HA gene of influenza viruses that circulated on Corsica Island between 2006-2010 showed for each season the presence of a dominant lineage characterized by at least one fixed mutation. The A/H3N2 and A/H1N1pdm isolates were characterized by multiples fixation at antigenic sites. The fixation of specific mutations at each outbreak could be explained by the combination of a neutral phenomenon and a founder effect, favoring the presence of a dominant lineage in a closed environment such as Corsica Island
NMR-Based Structural Modeling of Graphite Oxide Using Multidimensional 13C Solid-State NMR and ab Initio Chemical Shift Calculations
Chemically modified graphenes and other graphite-based materials have attracted growing interest for their unique potential as lightweight electronic and structural nanomaterials. It is an important challenge to construct structural models of noncrystalline graphite-based materials on the basis of NMR or other spectroscopic data. To address this challenge, a solid-state NMR (SSNMR)-based structural modeling approach is presented on graphite oxide (GO), which is a prominent precursor and interesting benchmark system of modified graphene. An experimental 2D C-13 double-quantum/single-quantum correlation SSNMR spectrum of C-13-labeled GO was compared with spectra simulated for different structural models using ab initio geometry optimization and chemical shift calculations. The results show that the spectral features of the GO sample are best reproduced by a geometry-optimized structural model that is based on the Lerf-Klinowski model (Lerf, A. et al. Phys. Chem. B 1998, 102, 4477); this model is composed of interconnected sp(2), 1,2-epoxide, and COH carbons. This study also convincingly excludes the possibility of other previously proposed models, including the highly oxidized structures involving 1,3-epoxide carbons (Szabo, I. et al. Chem. Mater. 2006, 18, 2740). C-13 chemical shift anisotropy (CSA) patterns measured by a 2D C-13 CSA/isotropic shift correlation SSNMR were well reproduced by the chemical shift tensor obtained by the ab initio calculation for the former model. The approach presented here is likely to be applicable to other chemically modified graphenes and graphite-based systems
HIV-1 Tat immunization restores immune homeostasis and attacks the HAART-resistant blood HIV DNA: results of a randomized phase II exploratory clinical trial
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