A Review On Geotrichum Lipases: Production, Purification, Immobilization And Applications

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Lipases are enzymes produced from innumerous microorganisms, plants and animal cells. They catalyze reactions of different lipid sources. The Geotrichum fungi are good producers of lipases with high hydrolytic activity and specificity for unsaturated fatty acids. A great number of studies have reported the importance of lipase from this genus and described important fermentation parameters for the enzyme production, such as nutrients, temperature, pH, inoculum, time of fermentation and others. Furthermore, different strategies have been used to purify and immobilize lipases from Geotrichum and innumerous applications are cited in different processes as polyunsaturated fatty acids enrichment, hydrolysis and esterification of fat and oils, synthesis of aromas, biodiesel, and many others. This review highlights fundamental aspects of the production, purification, characterization, immobilization, and the applications of lipases produced by the genus Geotrichum.304439454Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP, Brazil)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brazil)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES, Brazil)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

Strain balanced quantum posts

Quantum posts are assembled by epitaxial growth of closely spaced quantum dot layers, modulating the composition of a semiconductor alloy, typically InGaAs. In contrast with most self-assembled nanostructures, the height of quantum posts can be controlled with nanometer precision, up to a maximum value limited by the accumulated stress due to the lattice mismatch. Here we present a strain compensation technique based on the controlled incorporation of phosphorous, which substantially increases the maximum attainable quantum post height. The luminescence from the resulting nanostructures presents giant linear polarization anisotropy.Comment: Submitted to Applied Physics Letters (7th March 2011). 4 pages, 4 figure

Non-local scattering control in coupled resonator networks

We demonstrate scattering control of Gaussian-like wave packets propagating with constant envelope velocity and invariant waist through coupled resonator optical waveguides (CROW) via an external resonator coupled to multiple sites of the CROW. We calculate the analytical reflectance and transmittance using standard scattering methods from waveguide quantum electrodynamics and show it is possible to approximate them for an external resonator detuned to the CROW. Our analytical and approximate results are in good agreement with numerical simulations. We engineer various configurations using an external resonator coupled to two sites of a CROW to show light trapping with effective exponential decay between the coupling sites, wave packet splitting into two pairs of identical Gaussian-like wave packets, and a non-local Mach-Zehnder interferometer.Comment: 20 pages, 7 figure

GJ 357 b: A Super-Earth Orbiting an Extremely Inactive Host Star

In this paper we present a deep X-ray observation of the nearby M dwarf GJ 357 and use it to put constraints on the atmospheric evolution of its planet, GJ 357 b. We also analyse the systematic errors in the stellar parameters of GJ 357 in order to see how they affect the perceived planetary properties. We estimate the age of GJ 357 b by comparing the observed X-ray luminosity of its host star, derived from a recent {\em XMM-Newton} observation {($\log{L_{\rm x}}\,{\rm [erg/s]} = 25.73$), with $L_{\rm x} -$ age relations for M dwarfs. We find that GJ 357 presents one of the lowest X-ray activity levels ever measured for an M dwarf, and we put a lower limit on its age of $5$\,Gyr.} Using this age limit, we perform a backwards reconstruction of the original primordial atmospheric reservoir. Furthermore, by considering the systematic errors in the stellar parameters, we find a range of possible planetary masses, radii, and densities. From the backwards reconstruction of GJ 357 b's irradiation history we find that the upper limit of its initial primordial atmospheric mass is $\sim \rm 38M_{\oplus}$. An initial atmospheric reservoir significantly larger than this may have survived through the X-ray and ultraviolet irradiation history, hence being inconsistent with current observations that suggest a telluric composition. In spite of the unlikelihood of a currently existing primordial envelope, volcanism and outgassing may have contributed to a secondary atmosphere. Under this assumption, we present three different synthetic infrared spectra for GJ 357 b that one might expect, consisting of $100\%~\rm CO_{2}$, $100\%~\rm SO_{2}$, and $75\%~ \rm N_{2}$, $24\%~\rm CO_{2}$ and $1\%~\rm H_{2}O$.Comment: Accepted for publication in A&

A Review on Geotrichum Lipases: Production, Purification, Immobilization and Applications

Lipases are enzymes produced from innumerous microorganisms, plants and animal cells. They catalyze reactions of different lipid sources. The Geotrichum fungi are good producers of lipases with high hydrolytic activity and specificity for unsaturated fatty acids. A great number of studies have reported the importance of lipase from this genus and described important fermentation parameters for the enzyme production, such as nutrients, temperature, pH, inoculum, time of fermentation and others. Furthermore, different strategies have been used to purify and immobilize lipases from Geotrichum and innumerous applications are cited in different processes as polyunsaturated fatty acids enrichment, hydrolysis and esterification of fat and oils, synthesis of aromas, biodiesel, and many others. This review highlights fundamental aspects of the production, purification, characterization, immobilization, and the applications of lipases produced by the genus Geotrichum

Salmonella Pathogenesis and Processing of Secreted Effectors by Caspase-3

The enteric pathogen Salmonella enterica serovar Typhimurium causes food poisoning resulting in gastroenteritis. The S. Typhimurium effector Salmonella invasion protein A (SipA) promotes gastroenteritis by functional motifs that trigger either mechanisms of inflammation or bacterial entry. During infection of intestinal epithelial cells, SipA was found to be responsible for the early activation of caspase-3, an enzyme that is required for SipA cleavage at a specific recognition motif that divided the protein into its two functional domains and activated SipA in a manner necessary for pathogenicity. Other caspase-3 cleavage sites identified in S. Typhimurium appeared to be restricted to secreted effector proteins, which indicates that this may be a general strategy used by this pathogen for processing of its secreted effectors