Aviram-Ratner rectifying mechanism for DNA base pair sequencing through graphene nanogaps

We demonstrate that biological molecules such as Watson-Crick DNA base pairs can behave as biological Aviram-Ratner electrical rectifiers because of the spatial separation and weak hydrogen bonding between the nucleobases. We have performed a parallel computational implementation of the ab-initio non-equilibrium Green's function (NEGF) theory to determine the electrical response of graphene---base-pair---graphene junctions. The results show an asymmetric (rectifying) current-voltage response for the Cytosine-Guanine base pair adsorbed on a graphene nanogap. In sharp contrast we find a symmetric response for the Thymine-Adenine case. We propose applying the asymmetry of the current-voltage response as a sensing criterion to the technological challenge of rapid DNA sequencing via graphene nanogaps

The cognitive-affective-conative model of destination image: a confirmatory analysis

Destination image influences tourist behaviour before, during and after travel, as it is an important instrument which contributes to tourists’ loyalty. Although Gartner (1993) advocates that the cognitive, affective and conative dimensions of destination image are hierarchically interrelated, there is no empirical evidence to support the complete model. This study aims to test the hierarchical nature of the relationship between the dimensions of destination image. The results of structural equation modelling confirm Gartner’s theoretical model, validating the theory that the influence of the cognitive component on the conative dimension is higher when mediated by the affective component, raising managerial implications

The AFLOW Fleet for Materials Discovery

The traditional paradigm for materials discovery has been recently expanded to incorporate substantial data driven research. With the intent to accelerate the development and the deployment of new technologies, the AFLOW Fleet for computational materials design automates high-throughput first principles calculations, and provides tools for data verification and dissemination for a broad community of users. AFLOW incorporates different computational modules to robustly determine thermodynamic stability, electronic band structures, vibrational dispersions, thermo-mechanical properties and more. The AFLOW data repository is publicly accessible online at aflow.org, with more than 1.7 million materials entries and a panoply of queryable computed properties. Tools to programmatically search and process the data, as well as to perform online machine learning predictions, are also available.Comment: 14 pages, 8 figure

LBT observations of the HR 8799 planetary system: First detection of HR8799e in H band

We have performed H and Ks band observations of the planetary system around HR 8799 using the new AO system at the Large Binocular Telescope and the PISCES Camera. The excellent instrument performance (Strehl ratios up to 80% in H band) enabled detection the inner planet HR8799e in the H band for the first time. The H and Ks magnitudes of HR8799e are similar to those of planets c and d, with planet e slightly brighter. Therefore, HR8799e is likely slightly more massive than c and d. We also explored possible orbital configurations and their orbital stability. We confirm that the orbits of planets b, c and e are consistent with being circular and coplanar; planet d should have either an orbital eccentricity of about 0.1 or be non-coplanar with respect to b and c. Planet e can not be in circular and coplanar orbit in a 4:2:1 mean motion resonances with c and d, while coplanar and circular orbits are allowed for a 5:2 resonance. The analysis of dynamical stability shows that the system is highly unstable or chaotic when planetary masses of about 5 MJup for b and 7 MJup for the other planets are adopted. Significant regions of dynamical stability for timescales of tens of Myr are found when adopting planetary masses of about 3.5, 5, 5, and 5 Mjup for HR 8799 b, c, d, and e respectively. These masses are below the current estimates based on the stellar age (30 Myr) and theoretical models of substellar objects.Comment: 13 pages, 10 figures, A&A, accepte

PROP1 gene analysis in Portuguese patients with combined pituitary hormone deficiency

OBJECTIVE: Mutations of the PROP1 gene lead to combined pituitary hormone deficiency (CPHD), which is characterized by a deficiency of GH, TSH, LH/FSH, PRL and, less frequently, ACTH. This study was undertaken to investigate the molecular defect in a cohort of patients with CPHD. DESIGN, PATIENTS AND MEASUREMENTS: A multicentric study involving 46 cases of CPHD (17 familial cases belonging to seven kindreds and 29 sporadic cases) selected on the basis of clinical and hormonal evidence of GH deficiency, central hypothyroidism and hypogonadotrophic hypogonadism, in the absence of an identified cause of hypopituitarism. Mutations of PROP1 were investigated by DNA sequencing. Clinical, hormonal and neuroradiological data were collected at each centre. RESULTS: PROP1 mutations were identified in all familial cases: five kindreds presented a c. 301-302delAG mutation, one kindred presented a c. 358C --> T (R120C) mutation and one presented a previously unreported initiation codon mutation, c. 2T --> C. Of the 29 sporadic cases, only two (6.9%) presented PROP1 germline mutations (c. 301-302delAG, in both). Phenotypic variability was observed among patients with the same mutations, particularly the presence and age of onset of hypocortisolism, the levels of PRL and the results of pituitary imaging. One patient presented a sellar mass that persisted into adulthood. CONCLUSIONS: This is the first report of a mutation in the initiation codon of the PROP1 gene and this further expands the spectrum of known mutations responsible for CPHD. The low mutation frequency observed in sporadic cases may be due to the involvement of other unidentified acquired or genetic cause

Transverse Electronic Transport through DNA Nucleotides with Functionalized Graphene Electrodes

Graphene nanogaps and nanopores show potential for the purpose of electrical DNA sequencing, in particular because single-base resolution appears to be readily achievable. Here, we evaluated from first principles the advantages of a nanogap setup with functionalized graphene edges. To this end, we employed density functional theory and the non-equilibrium Green's function method to investigate the transverse conductance properties of the four nucleotides occurring in DNA when located between the opposing functionalized graphene electrodes. In particular, we determined the electrical tunneling current variation as a function of the applied bias and the associated differential conductance at a voltage which appears suitable to distinguish between the four nucleotides. Intriguingly, we observe for one of the nucleotides a negative differential resistance effect.Comment: 19 pages, 7 figure

xR-EgoPose: Egocentric 3D Human Pose from an HMD Camera

We present a new solution to egocentric 3D body pose estimation from monocular images captured from a downward looking fish-eye camera installed on the rim of a head mounted virtual reality device. This unusual viewpoint, just 2 cm away from the user's face, leads to images with unique visual appearance, characterized by severe self-occlusions and strong perspective distortions that result in a drastic difference in resolution between lower and upper body. Our contribution is two-fold. Firstly, we propose a new encoder-decoder architecture with a novel dual branch decoder designed specifically to account for the varying uncertainty in the 2D joint locations. Our quantitative evaluation, both on synthetic and real-world datasets, shows that our strategy leads to substantial improvements in accuracy over state of the art egocentric pose estimation approaches. Our second contribution is a new large-scale photorealistic synthetic dataset - xR-EgoPose - offering 383K frames of high quality renderings ofpeople with a diversity of skin tones, body shapes, clothing, in a variety of backgrounds and lighting conditions, performing a range of actions. Our experiments show that the high variability in our new synthetic training corpus leads to good generalization to real world footage and to state of the art results on real world datasets with ground truth. Moreover, an evaluation on the Human3.6M benchmark shows that the performance of our method is on par with top performing approaches on the more classic problem of 3D human pose from a third person viewpoint

CAD techniques for microwave circuits

In little more than 10 years computer-aided design (CAD) of microwave circuits has moved from dumb terminals on mainframe computers to PCs, and now to powerful RISC workstations. Commercial CAD software now integrates the various stages of microwave circuit design: schematic capture, simulation and layout. This paper reviews the different CAD packages that are available for microwave circuit design. The basic principles employed in the modelling of microstrip circuits are introduced and the reasons for the extensive use of frequency-domain simulations are explored. The developments in nonlinear, electromagnetic and system-level simulation methods are described.The authors would like to acknowledge the financial support of the Engineering and Physical Sciences Research Council, and the Comisidn Interministerial de Ciencia y Technologia (CICr?‘), Spain, under the project TIC95-0983-C03-02. We would like to thank Hewlett Packard, Barnard Microsystems, Sonnet Software, Optimization Systems Associates, Kimberley Communications Consultants and Ansoft Corporation for their generous educational discounting. The assistance of Dr. D. M. Brookbanks at GEC-Marconi Materials Technology Ltd. (Caswell) is gratefully acknowledged

CAD techniques for microwave circuits

In little more than 10 years computer-aided design (CAD) of microwave circuits has moved from dumb terminals on mainframe computers to PCs, and now to powerful RISC workstations. Commercial CAD software now integrates the various stages of microwave circuit design: schematic capture, simulation and layout. This paper reviews the different CAD packages that are available for microwave circuit design. The basic principles employed in the modelling of microstrip circuits are introduced and the reasons for the extensive use of frequency-domain simulations are explored. The developments in nonlinear, electromagnetic and system-level simulation methods are described.The authors would like to acknowledge the financial support of the Engineering and Physical Sciences Research Council, and the Comisidn Interministerial de Ciencia y Technologia (CICr?‘), Spain, under the project TIC95-0983-C03-02. We would like to thank Hewlett Packard, Barnard Microsystems, Sonnet Software, Optimization Systems Associates, Kimberley Communications Consultants and Ansoft Corporation for their generous educational discounting. The assistance of Dr. D. M. Brookbanks at GEC-Marconi Materials Technology Ltd. (Caswell) is gratefully acknowledged