174 research outputs found
Studies on \u3ci\u3eAttachment Behavior\u3c/i\u3e of Germinating Surface‐Sown Mengnong Sainfoin and \u3ci\u3eAstragalus adsurgens\u3c/i\u3e Seeds
Crash Prediction and Risk Evaluation Based on Traffic Analysis Zones
Traffic safety evaluation for traffic analysis zones (TAZs) plays an important role in transportation safety planning and long-range transportation plan development. This paper aims to present a comprehensive analysis of zonal safety evaluation. First, several criteria are proposed to measure the crash risk at zonal level. Then these criteria are integrated into one measure-average hazard index (AHI), which is used to identify unsafe zones. In addition, the study develops a negative binomial regression model to statistically estimate significant factors for the unsafe zones. The model results indicate that the zonal crash frequency can be associated with several social-economic, demographic, and transportation system factors. The impact of these significant factors on zonal crash is also discussed. The finding of this study suggests that safety evaluation and estimation might benefit engineers and decision makers in identifying high crash locations for potential safety improvements
Increased apoptosis of immunoreactive host cells and augmented donor leukocyte chimerism, not sustained inhibition of B7 molecule expression are associated with prolonged cardiac allograft survival in mice preconditioned with immature donor dendritic cells plus anti-CD40L mAb
Background. We previously reported the association among donor leukocyte chimerism, apoptosis of presumedly IL-2-deficient graft-infiltrating host cells, and the spontaneous donor-specific tolerance induced by liver but not heart allografts in mice. Survival of the rejection-prone heart allografts in the same strain combination is modestly prolonged by the pretransplant infusion of immature, costimulatory molecule-(CM) deficient donor dendritic cells (DC), an effect that is markedly potentiated by concomitant CM blockade with anti-CD40L (CD154) monoclonal antibody (mAb). We investigated whether the long survival of the heart allografts in the pretreated mice was associated with donor leukocyte chimerism and apoptosis of graft-infiltrating cells, if these end points were similar to those in the spontaneously tolerant liver transplant model, and whether the pretreatment effect was dependent on sustained inhibition of CM expression of the infused immature donor DC. In addition, apoptosis was assessed in the host spleen and lymph nodes, a critical determination not reported in previous studies of either spontaneous or 'treatment-aided' organ tolerance models. Methods. Seven days before transplantation of hearts from B10 (H-2b) donors, 2 x 106 donor- derived immature DC were infused i.v. into C3H (H-2(k)) recipient mice with or without a concomitant i.p. injection of anti-CD40L mAb. Donor cells were detected posttransplantation by immunohistochemical staining for major histocompatibility complex class II (I-Ab) in the cells of recipient lymphoid tissue. CM expression was determined by two-color labeling. Host responses to donor alloantigen were quantified by mixed leukocyte reaction, and cytotoxic T lymphocyte (CTL) assays. Apoptotic death in graft- infiltrating cells and in areas of T-dependent lymphoid tissue was visualized by terminal deoxynucleotidyltransferase-catalyzed dUTP-digoxigenin nick-end labeling and quantitative spectrofluorometry. Interleukin-2 production and localization were estimated by immunohistochemistry. Results. Compared with control heart transplantation or heart transplantation after only DC administration, concomitant pretreatment with immature donor DC and anti- CD40L mAb caused sustained elevation of donor (I-Ab+) cells (microchimerism) in the spleen including T cell areas. More than 80% of the I-Ab+ cells in combined treatment animals also were CD86+, reflecting failure of the mAb to inhibit CD40/CD80/CD86 up-regulation on immature DC in vitro after their interaction with host T cells. Donor-specific CTL activity in graft-infiltrating cells and spleen cell populations of these animals was present on day 8, but decreased strikingly to normal control levels by day 14. The decrease was associated with enhanced apoptosis of graft-infiltrating cells and of cells in the spleen where interleukin-2 production was inhibited. The highest levels of splenic microchimerism were found in mice with long surviving grafts (> 100 days). In contrast, CTL activity was persistently elevated in control heart graft recipients with comparatively low levels of apoptotic activity and high levels of interleukin-2. Conclusion. The donor-specific acceptance of rejection-prone heart allografts by recipients pretreated with immature donor DC and anti-CD40L mAb is not dependent on sustained inhibition of donor DC CM (CD86) expression. Instead, the pretreatment facilitates a tolerogenic cascade similar to that in spontaneously tolerant liver recipients that involves: (1) chimerism-driven immune activation, succeeded by deletion of host immune responder cells by apoptosis in the spleen and allograft that is linked to interleukin-2 deficiency in both locations and (2) persistence of comparatively large numbers of donor-derived leukocytes. These tolerogenic mechanisms are thought to be generic, explaining the tolerance induced by allografts spontaneously, or with the aid of various kinds of immunosuppression
Purification and characterization of a novel defensin from the salivary glands of the black fly, Simulium bannaense
BACKGROUND: Black flies (Diptera: Simuliidae) are haematophagous insects that can cause allergic reactions and act as vectors of pathogens. Although their saliva has been thought to contain a diverse array of physiologically active molecules, little information is available on antimicrobial factors in black fly salivary glands, especially no defensins have been reported so far. METHODS: A novel cationic defensin designated SibaDef was purified using reverse phase high-performance liquid chromatography (RP-HPLC) from the salivary glands of the black fly Simulium bannaense. The amino acid sequence of SibaDef was determined by a combination method of automated Edman degradation and cDNA sequencing. The morphologic changes of Gram-positive bacteria Staphylococcus aureus or Bacillus subtilis treated with SibaDef were assessed by scanning electron microscopy (SEM). Quantitative PCR (qPCR) was performed to analyze the expression of SibaDef mRNA in whole bodies of insects after oral infection with the bacteria S. aureus or B. subtilis. RESULTS: Surprisingly, the phylogenetic analysis of defensin-related amino acid sequences demonstrated that SibaDef is most closely related to defensins from the human body louse Pediculus humanus corporis (Anoplura: Pediculidae), rather than to other dipteran defensins. SibaDef showed potent antimicrobial activities against Gram-positive bacteria with minimal inhibitory concentrations (MICs) ranging from 0.83 μM to 2.29 μM. SEM analysis indicated that SibaDef killed microorganisms through the disruption of cell membrane integrity. The transcript levels of SibaDef in the bacteria-immunized flies increased with the time course, reaching maximum at 36 h and then slowly decreased from that time point. CONCLUSIONS: Our results indicate that SibaDef is involved in the innate humoral response of the black fly S. bannaense, and it might play a significant role in the defence against microorganisms in both sugar and blood meals
Study on the construction technology of β-alanine synthesizing Escherichia coli based on cellulosome assembly
Introduction: β-Alanine is the only β-amino acid in nature; it is widely used in food additives, medicines, health products, and surfactants. To avoid pollution caused by traditional production methods, the synthesis of β-alanine has been gradually replaced by microbial fermentation and enzyme catalysis, which is a green, mild, and high-yield biosynthesis method.Methods: In this study, we constructed an Escherichia coli recombinant strain for efficient β-alanine production using glucose as the raw material. The microbial synthesis pathway of L-lysine-producing strain, Escherichia coli CGMCC 1.366, was modified using gene editing by knocking out the aspartate kinase gene, lysC. The catalytic efficiency and product synthesis efficiency were improved by assembling key enzymes with cellulosome.Results: By-product accumulation was reduced by blocking the L-lysine production pathway, thereby increasing the yield of β-alanine. In addition, catalytic efficiency was improved by the two-enzyme method to further increase the β-alanine content. The key cellulosome elements, dockerin (docA) and cohesin (cohA), were combined with L-aspartate-α-decarboxylase (bspanD) from Bacillus subtilis and aspartate aminotransferase (aspC) from E.coli to improve the catalytic efficiency and expression level of the enzyme. β-alanine production reached 7.439 mg/L and 25.87 mg/L in the two engineered strains. The β-alanine content reached 755.465 mg/L in a 5 L fermenter.Discussion: The content of β-alanine synthesized by constructed β-alanine engineering strains were 10.47 times and 36.42 times higher than the engineered strain without assembled cellulosomes, respectively. This research lays the foundation for the enzymatic production of β-alanine using a cellulosome multi-enzyme self-assembly system
Parathyroid Hormone-Related Protein (1-40) Enhances Calcium Uptake in Rat Enterocytes Through PTHR1 Receptor and Protein Kinase Cα/β Signaling
Background/Aims: Parathyroid hormone-related protein (PTHrP) is implicated in regulating calcium homeostasis in vertebrates, including sea bream, chick, and mammals. However, the molecular mechanism underlying the function of PTHrP in regulating calcium transport is still not fully investigated. This study aimed to investigate the effect of PTHrP on the calcium uptake and its underlying molecular mechanism in rat enterocytes. Methods: The rat intestinal epithelial cell line (IEC-6) was used. Calcium uptake was determined by using the fluo-4 acetoxymethyl ester fluorescence method. The expression levels of RNAs and proteins was assessed by RT-PCR and Western-blot, respectively. Results: PTHrP (1-40) induced rapid calcium uptake in enterocytes in a dose-dependent manner. PTHrP (1-40) up-regulated parathyroid hormone 1 receptor (PTHR1) protein but not 1,25D3-MARRS receptor. Pre-treatment of anti- PTHR1 antibody abolished the PTHrP (1-40)-induced calcium uptake. PTHrP (1-40) significantly up-regulated four transcellular calcium transporter proteins, potential vanilloid member 6 (TRPV6), calbindin-D9k (CaBP-D9k), sodium-calcium exchanger 1 (NCX1) and plasma membrane calcium ATPase 1 (PMCA1), in a dose- and time-dependent manner. Pre-treatment with TRPV6 or CaBP-D9k antibodies or knockout of rat TRPV6 or CaBP-D9k markedly inhibited PTHrP (1-40)-induced calcium uptake, whereas inhibition of NCX or PMCA1 by antibodies or inhibitors had no effect on PTHrP(1-40)-induced calcium uptake. Furthermore, PTHrP (1-40) treatment up-regulated protein levels of protein kinase C (PKC α/β) and protein kinase A (PKA). Pretreatment of PKC α/β inhibitor (but not PKA inhibitor) inhibited PTHrP (1-40)-induced calcium uptake. Conclusion: PTHrP (1-40) stimulates calcium uptake via PTHR1 receptor and PKC α/β signaling pathway in rat enterocytes, and calcium transporters TRPV6 and CaBP-D9k are necessary for this stimulatory effect
A strategy for designing microencapsulated composite phase change thermal storage materials with tunable melting temperature
Abstract(#br)Thermal energy storage technology with high temperature phase change materials (PCMs) plays an increasingly important role in the concentrated solar power plants and industrial waste heat recovery systems. In this study, a novel displacement reaction between tetraethoxysilane as SiO 2 source and molten raw Al powder was purposed to successfully prepare Al-Si/Al 2 O 3 high temperature composite PCMs. Interestingly, by proposed synthetic methodology, we not only achieved the in-situ synthesis of Al-Si alloy PCM and Al 2 O 3 shell, but also realized the controllability of Al-Si alloy composition and Al 2 O 3 shell layer thickness. Our results indicated that the melting temperature of the prepared composite PCMs depended on the composition of Al-Si alloy, and could be designed within a certain temperature range (from 574.0 °C to 641.4 °C), instead of a particular temperature point. The melting temperature adjustability of the prepared composite PCMs provided an additional flexibility in different working temperature conditions. Moreover, the prepared composite PCMs exhibited a relatively high thermal storage capacity (248.6 J/g to 331.0 J/g), good thermal stability, excellent repeatable utilization property and certain shell layer self-repairing ability in the working temperature range. Therefore, the prepared composite PCMs can prove to be promising thermal energy storage materials for improving the energy efficiency in various systems under different working temperature conditions
Novel core/void/shell composite phase change materials for high temperature thermal energy storage
Abstract(#br)Metallic solid-liquid phase change materials (SLPCMs) are crucial for the thermal energy storage technology of various industrial systems. However, the encapsulation of metallic SLPCMs is still technically difficult. In this pursuit, the present research envisaged the development of a novel technology to successfully prepare the core(=Al-Si/Bi)/void/shell(=Al 2 O 3 ) composite SLPCMs by using Al/Bi immiscible alloy powders as starting material and tetraethoxysilane as SiO 2 source. The Al-Si alloy and Al 2 O 3 shell were in-situ synthesized by the displacement reaction between SiO 2 and molten Al. Interestingly, most of the Bi distributed in the shell of Al/Bi immiscible alloy powders could not only improve the activity of alloy powders and promote the formation of precursor shell, but also be recycled by evaporation to form the void layer during the calcination process of composite SLPCMs. The produced void layer provided a space buffer to alleviate the volume expansion of the core SLPCM, and thereby improving the thermal cycling stability of the prepared composite SLPCMs. The thermal cycling test results showed that after 300 thermal cycles, the melting latent heat reduction of the core(=Al-Si/Bi)/void/shell(=Al 2 O 3 ) composite SLPCMs (24.3-31.7J/g) was much less than that of the core(=Al-Si)/shell(=Al 2 O 3 ) composite SLPCM (58.1J/g). Moreover, the prepared Al-Si/Bi/Al 2 O 3 exhibited an adjustable melting temperature (571.9℃ to 631.9℃) and average particle diameter (39.3μm to 112.6μm), relatively high thermal conductivity [2.068W(mK) -1 to 2.966W(mK) -1 ], and excellent thermal energy storage capacity (209.5J/g to 278.2J/g). Thus, the prepared Al-Si/Bi/Al 2 O 3 composite SLPCMs are potential thermal energy storage materials, which can be used to improve the energy efficiency of various industrial systems
Effect of Re on microstructure and mechanical properties of γ/γʹ Co-Ti-based superalloys
Abstract(#br)Three compositions of Re containing Co-Ti-based alloys with γ/γ′ two-phase microstructure were obtained based on the determined phase relationship in the Co-rich corner at 800 °C. The effect of Re on microstructure, elemental partition behavior, thermodynamic properties, lattice parameter misfits, mechanical properties and γ′ coarsening behavior of the Co-Ti-Re alloys were investigated. The γ/γ′ lattice parameter misfit of the alloys decreases from 0.72% to 0.50%, and the γ′ coarsening rate constant K of alloys decreases from 0.30 × 10 −27 m 3 s −1 to 0.073 × 10 −27 m 3 s −1 as Re content increasing form 1 at. % to 5 at. %. Additionally, the volume fraction of γ′ phase (V γ′ ) increases from 42.15% to 53.19% and the γ′ solvus temperature increases from 1059 °C to 1085 °C ( T solvus-γ′ ) with increasing Re content. Moreover, the 0.2% flow stresses and the 0.2% specific flow stresses of all three alloys exhibit negative temperature dependence at the temperatures ranging from room temperature to 900 °C. And both the 0.2% flow stresses and the 0.2% specific flow stresses increase with the Re content
Experimental Investigation of Diagram Equilibria in the Co-Nb-Re Ternary System
Abstract(#br)In this study, the isothermal sections of the Co-Nb-Re ternary system at 1200, and 1300 °C have been experimentally determined combining the means of electron probe microanalysis (EPMA) and x-ray diffraction (XRD). The obtained experimental results showed that: (1) The Laves phase of λ3-Co2Nb (C36) was stable at 1300 °C. The temperature was beyond its stability limit in Co-Nb binary system. (2) The solubility of Re in the λ3 phase was so large that the nearest λ2-Co2Nb (C15) phase was essentially surrounded. (3) The solubility of Re in the μ-Co7Nb6 phase was 34.0 at.% at 1200 °C and 35.2 at.% at 1300 °C, respectively. (4) The liquid phase existed at 1300 °C dissolving about 4.0 at.% Re, but it was..
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