ESTUDIO DE LA CALIDAD DE VIDA RELACIONADA CON LA SALUD, ANSIEDAD, DEPRESIÓN Y CALIDAD DEL SUEÑO EN PERSONAS DE MEDIANA Y AVANZADA EDAD, Y SU RELACIÓN CON LA SARCOPENIA, OBESIDAD Y OBESIDAD SARCOPÉNICA.

En esta tesis doctoral se analizaron en primer lugar las propiedades psicométricas de la versión española del cuestionario de calidad de vida específico de sarcopenia (SarQoL®) en adultos ≥65 años, que mostró una buena consistencia interna, fiabilidad test-retest, validez concurrente y divergente, y capacidad discriminativa entre participantes con y sin sarcopenia. Posteriormente, se estudió la sarcopenia y obesidad en adultos mayores y su asociación con la calidad de vida relacionada con la salud (CVRS), duración y calidad del sueño, ansiedad y depresión, mostrando las siguientes asociaciones independientes: i) Menor fuerza muscular y velocidad de la marcha y mayor obesidad general se relacionaron con peor CVRS. ii) Mayores síntomas depresivos con sarcopenia, y peor calidad del sueño con mayor obesidad general (también con menor duración del sueño) y abdominal. iii) Peor calidad del sueño con menor velocidad de la marcha y mayor ansiedad con menor masa muscular.In this doctoral thesis the psychometric properties of the Spanish version of the sarcopenia specific quality of life questionnaire (SarQoL®) were firstly performed in ≥ 65 years old adults, showing good internal consistency, test-retest reliability, concurrent validity and divergent, and been able to discriminate between participants with and without sarcopenia. Secondly, sarcopenia and obesity and their association with health-related quality of life (HRQOL), sleep quality and duration, and the level of anxiety and depression, showing the following independent associations: i) Lower muscle strength and gait speed were related to worse HRQOL. ii) Greater depressive symptoms were associated with sarcopenia and worse sleep quality were linked to greater general (together with lower sleep duration) and abdominal obesity. iii) worse sleep quality and higher levels of anxiety were associated with lower gait speed and decreased muscle mass respectively.Tesis Univ. Jaén. Departamento de Ciencias de la Salud. Leída el 5 de marzo de 2020

Gestión administrativa y calidad de servicio del usuario en un hospital nivel II, Lima - 2023

La investigación tuvo como objetivo general determinar la relación entre la gestión administrativa y calidad de servicio del usuario en un hospital nivel II, Lima - 2023. La investigación fue cuantitativa, no experimental y correlacional, se estableció una muestra de 162 usuarios por intermedio de un muestreo probabilístico, el instrumento correspondió al cuestionario, la validez fue a través de juicio de expertos y la confiabilidad usando el coeficiente Alfa de Cronbach, para gestión administrativa se encontró un α = 0,862 y para calidad de servicio un α = 0,879. Los resultados muestran un coeficiente Rho de Spearman = 0.923, significa una correlación positiva perfecta entre gestión administrativa y calidad de servicio. Además, se halló un valor de p=0.000 < a 0,05, por tanto, se logró aceptar la hipótesis alterna (Ha). En consecuencia, se concluyó una correlación positiva perfecta entre la gestión administrativa y la calidad de servicio, es decir, en la medida que se mejore la gestión administrativa, se podrá mejorar la calidad de servicio en un hospital nivel II

Phosphorylation and dephosphorylation of tau protein by the catalytic subunit of PKA, as probed by electrophoretic mobility retard

Background: Tau is a microtubule associated protein that regulates the stability of microtubules and the microtubule-dependent axonal transport. Its hyperphosphorylated form is one of the hallmarks of Alzheimer's disease and other tauopathies and the major component of the paired helical filaments that form the abnormal proteinaceous tangles found in these neurodegenerative diseases. It is generally accepted that the phosphorylation extent of tau is the result of an equilibrium in the activity of protein kinases and phosphatases. Disruption of the balance between both types of enzyme activities has been assumed to be at the origin of tau hyperphosphorylation and the subsequent toxicity and progress of the disease. Objective: We explore the possibility that, beside the phosphatase action on phosphorylated tau, the catalytic subunit of PKA catalyzes both tau phosphorylation and also tau dephosphorylation, depending on the ATP/ADP ratio. Methods: We use the shift in the relative electrophoretic mobility suffered by different phosphorylated forms of tau, as a sensor of the catalytic action of the enzyme. Results: The results are in agreement with the long-known thermodynamic reversibility of the phosphorylation reaction (ATP + Protein = ADP+Phospho-Protein) catalyzed by PKA and many other protein kinases. Conclusion: The results contribute to put the compartmentalized energy state of the neuron and the mitochondrial-functions disruption upstream of tau-related pathologiesFinancial support has been provided by a grant from the Spanish Government PGC2018 096177-B-10

Specific Profile of Tau Isoforms in Argyrophylic Grain Disease

Argyrophylic grain disease (AGD) is a neurodegenerative condition that has been classified among the sporadic tauopathies. Entities in this group present intracellular aggregates of hyperphosphorylated tau, giving rise to characteristic neuronal and glial inclusions. In different tauopathies, the proportion of several tau isoforms present in the aggregates shows specific patterns. AGD has been tentatively classified in the 4R group (predominance of 4R tau isoforms) together with progressive supranuclear palsy and corticobasal degeneration. Pick's disease is included in the 3R group (predominance of 3R isoforms), whereas tau pathology of Alzheimer's disease represents and intermediate group (3 or 4 repeats [3R plus 4R, respectively] isoforms). In this work, we have analyzed tau present in aggregates isolated from brain samples of patients with argyrophylic grain disease. Our results indicate that the main tau isoform present in aggregates obtained from patients with AGD is a hyperphosphorylated isoform containing exons 2 and 10 but lacking exon 3.This work was supported by grants from Spanish Plan Nacional (SAF2011-24841), CIBERNED and Reina Sofia Foundation.S

Tridimensional Structural Analysis of Tau Isoforms Generated by Intronic Retention.

Background: Tauopathies are a subset of neurodegenerative diseases characterized by abnormal tau inclusions. Recently, we have discovered a new, human specific, tau isoform termedW-tau that originates by intron 12 retention. Our preliminary data suggests this newly discovered W-tau isoform might prevent aberrant aggregation of other tau isoforms but is significantly downregulated in tauopathies such as Alzheimer´s disease. Objective: To accurately predict, examine, and understand tau protein structure and the conformational basis for the neuroprotective role of W-tau. Methods: A tridimensional deep learning-based approach and in vitro polymerization assay was included to accurately predict, analyze, and understand tau protein structure and the conformational basis for the neuroprotective role of W-tau. Results: Our findings demonstrate: a) the predicted protein tridimensionality structure of the tau isoforms raised by intron retention and their comparison with the other tau isoforms; b) the interaction of W-tau peptide (from W-tau isoform) with other tau isoforms; c) the effect of W-tau peptide in the polymerization of those tau isoforms. Conclusions: This study supports the importance of the structure-function relationship on the neuroprotective behavior of W-tau inhibiting tau fibrillization in vitro.post-print436 K

Propuesta pedagógica para el desarrollo de habilidades básicas de pensamiento a partir de la literatura para niños de 4 a 5 años

266 páginasEste estudio se enfoca en diseñar una propuesta pedagógica que utilice la literatura infantil como herramienta para desarrollar habilidades básicas de pensamiento en niños de 4 a 5 años. Inspirado por Gianni Rodari, Loris Malaguzzi y Rocío Vélez de Piedrahíta, busca crear un ambiente educativo enriquecedor que estimule la imaginación, la creatividad y el pensamiento crítico desde una edad temprana. Se emplea la metodología de investigación-acción en colaboración con maestros, padres y cuidadores para diseñar e implementar estrategias educativas innovadoras y contextualizadas. Los resultados resaltan un impacto considerable en el desarrollo cognitivo, emocional y social de los niños, preparándolos para afrontar desafíos de manera sólida y efectiva.Maestría en Desarrollo InfantilMagíster en Desarrollo Infanti

Effects of self-efficacy, catastrophizing, fear of movement and joint position sense in the maintenance of wrist unspecific pain in athletes. An observational study.

Wrist pain is a highly prevalent condition in racket sport population. Although different wrist injuries have been described in the literature as more prevalent in racket sport, unspecific chronic wrist pain is common problem on amateur population. Material and methods: A cross-sectional study were conducted between October 2021 to June 2022. Participants over 18 th suffered for wrist pain were recruited from different sports centers where racket sports were practiced. Objective: The aims of this research was to study the relationships and the predictive power of Self-Efficacy, Catastrophizing, Fear of Movement and proprioception on the persistence of unspecific wrist pain in athlete population. Results: One hundred and four athletes participated in the study (54 men and 50 women), most of them tennis and paddle players. Psychological variables were correlated (P 0.01) with wrist pain persistence, suggestive of complex interactions between cognitive-affective processes and wrist unspecific pain in athletes. Conclusion: The results suggest that targeting high levels of Self-Efficacy, Catastrophizing and Fear of Movement could be a prognostic factor of wrist unspecific maintained pain in athlete population.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Specific Peptide from the Novel W-Tau Isoform Inhibits Tau and Amyloid β Peptide Aggregation in Vitro

W-Tau, a new tau human-specific splicing isoform generated by intron retention, has been recently described. This isoform contains an 18-residue unique sequence corresponding to the translation of the retained region of intron 12. In this work, we have described that such 18-amino-acid peptide from the retained intron 12 can inhibit tau and β amyloid peptides aggregation under in vitro conditions. This inhibitory function is also present in smaller fragments of the 18-residue peptide.This research was funded by the Spanish Ministry of Science and Innovation (BES-2015-074405 and PGC2018-096177-BI00), the Center for Networked Biomedical Research on Neurodegenerative Diseases, and the Comunidad de Madrid (S2017/BMD). Institutional grants from the Fundación Ramón Areces and Banco de Santander are also acknowledged

Predictive Biomarkers to Chemoradiation in Locally Advanced Rectal Cancer

There has been a high local recurrence rate in rectal cancer. Besides improvements in surgical techniques, both neoadjuvant short-course radiotherapy and long-course chemoradiation improve oncological results. Approximately 40–60% of rectal cancer patients treated with neoadjuvant chemoradiation achieve some degree of pathologic response. However, there is no effective method of predicting which patients will respond to neoadjuvant treatment. Recent studies have evaluated the potential of genetic biomarkers to predict outcome in locally advanced rectal adenocarcinoma treated with neoadjuvant chemoradiation. The articles produced by the PubMed search were reviewed for those specifically addressing a genetic profile’s ability to predict response to neoadjuvant treatment in rectal cancer. Although tissue gene microarray profiling has led to promising data in cancer, to date, none of the identified signatures or molecular markers in locally advanced rectal cancer has been successfully validated as a diagnostic or prognostic tool applicable to routine clinical practice

Explorando la Implementación de un Laboratorio Remoto de Impresión 3D para la Enseñanza de las TIC

El uso de las Tecnologías de la Información y la Comunicación, incluida la impresión 3D, es esencial en la vida moderna, pero su implementación en escuelas se enfrenta a limitaciones como el tiempo, el desperdicio de material y la accesibilidad. Los laboratorios remotos ofrecen una solución al brindar acceso a diversos recursos, mejorando la experiencia de aprendizaje para estudiantes. Se evaluó un laboratorio de impresión 3D proporcionado por LabsLand en centros de secundaria, utilizando un diseño pre/post test. Los resultados demostraron que el laboratorio remoto ayuda a los alumnos a comprender esta tecnología, especialmente en términos de orientación de impresión. La experiencia de usuario también fue medida y los estudiantes mostraron una gran satisfacción, lo que les ayudó a aprender mejor la tecnología. La integración de estos laboratorios remotos puede potenciar la educación en TIC en las escuelas y superar las limitaciones tradicionales