A century of limnological research in the Historical Archive of the CNR ISE

Since 2010, the Historical Archive of the Italian Institute of Ecosystem Studies has been subject to an extensive rearrangement and improvement program. The Archive contains original documents (such as letters, administrative documents, notarial deeds, drawings and prints, photographic plates, photographs) recording the scientific and administrative activities from the foundation of the Institute (1938) till its annexation to the Italian National Research Council (1977). Furthermore, it hosts some material documenting studies that were conducted in the first decades of the XIX Century, mainly by Marco De Marchi (1872-1936), a pioneer of limnology in Italy, and Edgardo Baldi (1899-1951), the first director of the Institute. This collection is a cornerstone of more than a century of scientific research in limnology, which also benefitted from frequent international contacts with scientists and ecological associations. The reorganization of the material in the Historical Archive was planned in consultation with the Soprintendenza Archivistica of the Piedmont Region, which is the legal authority responsible for the public archives in the Piedmont Region. Beginning in 2012, the information contained in the Archive has been digitized using the software xDams, an open version software source of Regesta?. In 2014, part of the digitized material was made available on the web site: http://www.ise.cnr.it/archivio. These activities were conducted thanks to the contribution of the Fondazione Cassa Risparmio di Torino and Fondazione Comunitaria VCO, which funded two temporary staff for the reorganisation of the Historical Archive, including the physical preservation and digitalisation of the documents. These activities are part of the CNR mission within the European Digital Agenda, and the CNR contribution to the development of scientific thought

Reutilización de aguas residuales tratadas en cultivos forrajeros y forestales en Ing. Jacobacci

El uso de aguas residuales tratadas (ART) en riego agrícola forestal es una alternativa para evitar el vuelco a cuerpos receptores hídricos, que resulta de especial interés en áreas donde el agua es escasa. Resultado de un Convenio entre el DPA, la UNR, el INTA, la Municipalidad y la Cooperativa de Aguas, se comenzó en 2015 un trabajo conjunto en la localidad de Ingeniero Jacobacci con el objetivo de diseñar y probar estrategias para el mejor uso de esas aguas residuales, con la idea que el modelo desarrollado pueda ser aplicado en otras localidades de la región. Para ello se realizaron talleres en diferentes ámbitos de la comunidad para difundir la temática y recoger percepciones y temores preexistentes acerca de la misma. Además, se instaló en febrero de 2016 un ensayo de reutilización de ART para la producción de forraje (alfalfa y campo natural) y biomasa (olivillo y sauce) en la planta de tratamiento, con el objetivo de evaluar el impacto en las propiedades del suelo y en la productividad vegetal. En la producción de forraje se observó en alfalfa un rendimiento muy superior a lo esperado en la región, no observándose diferencias significativas entre los tratamientos (promedio de 24 Ton/ha en tres cortes en el primer año y de casi 50 Ton/ha en cuatro cortes en el segundo). Por efecto del riego, en el suelo se observaron cambios en algunos parámetros edáficos como una reducción del pH en agua y un incremento en la conductividad eléctrica en las parcelas regadas con agua tratada. También se observaron incrementos en las cargas de nutrientes aunque los valores máximos observados no alcanzaron límites que impliquen riesgos ambientales. Los ensayos de especies forestales comenzarán a evaluarse en la próxima temporada. Estos resultados, si bien son preliminares y en condiciones de cultivo muy difícilmente alcanzables en una producción comercial, reflejan buenas posibilidades de producir material seca vegetal con la aplicación de agua tratada, aunque se requiere planificar un riguroso control del impacto ambiental.Estación Experimental Agropecuaria BarilocheFil: Cremona, Maria Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; ArgentinaFil: Riat, Martha. Universidad Nacional de Rio Negro; ArgentinaFil: Magnin, Santiago. Departamento Provincial de Aguas. Rio Negro; ArgentinaFil: Velasco, Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Agencia de Extensión Rural Jacobacci; ArgentinaFil: Sanchez, V. Universidad Nacional de Rio Negro; ArgentinaFil: Tanzer, Laura. Departamento Provincial de Aguas. Rio Negro; Argentin

ILDR2: An Endoplasmic Reticulum Resident Molecule Mediating Hepatic Lipid Homeostasis

Ildr2, a modifier of diabetes susceptibility in obese mice, is expressed in most organs, including islets and hypothalamus, with reduced levels in livers of diabetes-susceptible B6.DBA mice congenic for a 1.8 Mb interval of Chromosome 1. In hepatoma and neuronal cells, ILDR2 is primarily located in the endoplasmic reticulum membrane. We used adenovirus vectors that express shRNA or are driven by the CMV promoter, respectively, to knockdown or overexpress Ildr2 in livers of wild type and ob/ob mice. Livers in knockdown mice were steatotic, with increased hepatic and circulating triglycerides and total cholesterol. Increased circulating VLDL, without reduction in triglyceride clearance suggests an effect of reduced hepatic ILDR2 on hepatic cholesterol clearance. In animals that overexpress Ildr2, hepatic triglyceride and total cholesterol levels were reduced, and strikingly so in ob/ob mice. There were no significant changes in body weight, energy expenditure or glucose/insulin homeostasis in knockdown or overexpressing mice. Knockdown mice showed reduced expression of genes mediating synthesis and oxidation of hepatic lipids, suggesting secondary suppression in response to increased hepatic lipid content. In Ildr2-overexpressing ob/ob mice, in association with reduced liver fat content, levels of transcripts related to neutral lipid synthesis and cholesterol were increased, suggesting “relief” of the secondary suppression imposed by lipid accumulation. Considering the fixed location of ILDR2 in the endoplasmic reticulum, we investigated the possible participation of ILDR2 in ER stress responses. In general, Ildr2 overexpression was associated with increases, and knockdown with decreases in levels of expression of molecular components of canonical ER stress pathways. We conclude that manipulation of Ildr2 expression in liver affects both lipid homeostasis and ER stress pathways. Given these reciprocal interactions, and the relatively extended time-course over which these studies were conducted, we cannot assign causal primacy to either the effects on hepatic lipid homeostasis or ER stress responses

Convenio de cooperación técnica: Reutilización agrícola forestal de aguas tratadas en Ingeniero Jacobacci

El uso de aguas residuales tratadas en riego agrícola forestal es una alternativa para evitar el vuelco a cuerpos receptores hídricos, que resulta de especial interés en áreas donde la escasez de agua afecta las actividades humanas. La zona centro de la provincia de Río Negro integra los dos tercios de la superficie en la Argentina que presenta balances hídricos negativos la mayor parte del año, esto representa una importante limitación para todo tipo de actividades productivas y en muchos casos hasta se dificulta el abastecimiento de agua para consumo humano.Estación Experimental Agropecuaria BarilocheFil: Cremona, Maria Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Recursos Naturales; ArgentinaFil: Velasco, Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Agencia de Extensión Rural Ingeniero Jacobacci; ArgentinaFil: Zuñiga, Aldo Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Recursos Naturales; ArgentinaFil: Tanzer, Laura. Departamento Provincial de Aguas. Rio Negro; ArgentinaFil: Magnin, Santiago. Departamento Provincial de Aguas. Rio Negro; ArgentinaFil: Fernandez, Patricia. Departamento Provincial de Aguas. Rio Negro; ArgentinaFil: Alemanni, Maria Eugenia. Departamento Provincial de Aguas. Rio Negro; ArgentinaFil: Riat, Martha. Universidad Nacional de Rio Negro; ArgentinaFil: Laos, Francisca. Universidad Nacional de Rio Negro; ArgentinaFil: Capuano, Ana. Universidad Nacional de Rio Negro; ArgentinaFil: Güevara, Tomas. Universidad Nacional de Rio Negro; ArgentinaFil: Fornasa, Alejandro. Cooperativa de agua y otros servicios públicos de Ingeniero Jacobacci; ArgentinaFil: Mellado, José. Cooperativa de agua y otros servicios públicos de Ingeniero Jacobacci; ArgentinaFil: López, Oscar. Cooperativa de agua y otros servicios públicos de Ingeniero Jacobacci; ArgentinaFil: Currumán, Antonio. Municipalidad de Ingeniero Jacobacci; ArgentinaFil: Nasif, Abdel. Ente para el Desarrollo de la Línea y región Sur; Argentin

'From mosh pit to posh pit': Festival imagery in the context of the boutique festival

This paper addresses market-based cultural production in the context of the UK festival field, with a focus on the framing of the festival experience through anticipation. In particular, boutique festivals are discussed as examples of a contemporary cultural ?product category? which has emerged and proliferated in the last decade. Through discourse analysis of media representations of boutique festivals, we situate the boutique festival in a broader sociocultural discourse of agency and choice, which makes it meaningful and desirable, and outline the type of consumer it is meant to attract. For the contemporary consumer, the boutique festival is presented as an anticipated experience based on countercultural festival imagery, while simultaneously framing cultural participation through consumption. The paper contributes to a wider debate on the construction of the consumer in the cultural economy

RBD-specific polyclonal F(ab´)2 fragments of equine antibodies in patients with moderate to severe COVID-19 disease: A randomized, multicenter, double-blind, placebo-controlled, adaptive phase 2/3 clinical trial

Background: passive immunotherapy is a therapeutic alternative for patients with COVID-19. Equine polyclonal antibodies (EpAbs) could represent a source of scalable neutralizing antibodies against SARS-CoV-2. Methods: we conducted a double-blind, randomized, placebo-controlled trial to assess efficacy and safety of EpAbs (INM005) in hospitalized adult patients with moderate and severe COVID-19 pneumonia in 19 hospitals of Argentina. Primary endpoint was improvement in at least two categories in WHO ordinal clinical scale at day 28 or hospital discharge (ClinicalTrials.gov number NCT04494984). Findings: between August 1st and October 26th, 2020, a total of 245 patients were enrolled. Enrolled patients were assigned to receive two blinded doses of INM005 (n = 118) or placebo (n = 123). Median age was 54 years old, 65 1% were male and 61% had moderate disease at baseline. Median time from symptoms onset to study treatment was 6 days (interquartile range 5 to 8). No statistically significant difference was noted between study groups on primary endpoint (risk difference [95% IC]: 5 28% [-3 95; 14 50]; p = 0 15). Rate of improvement in at least two categories was statistically significantly higher for INM005 at days 14 and 21 of follow-up. Time to improvement in two ordinal categories or hospital discharge was 14 2 (§ 0 7) days in the INM005 group and 16 3 (§ 0 7) days in the placebo group, hazard ratio 1 31 (95% CI 1 0 to 1 74). Subgroup analyses showed a beneficial effect of INM005 over severe patients and in those with negative baseline antibodies. Overall mortality was 6 9% the INM005 group and 11 4% in the placebo group (risk difference [95% IC]: 0 57 [0 24 to 1 37]). Adverse events of special interest were mild or moderate; no anaphylaxis was reported. Interpretation: Albeit not having reached the primary endpoint, we found clinical improvement of hospitalized patients with SARS-CoV-2 pneumonia, particularly those with severe disease.Fil: Lopardo, Gustavo. Municipalidad de Vicente Lopez (buenos Aires). Hospital Municipal Doctor Bernardo Houssay.; ArgentinaFil: Belloso, Waldo H.. Hospital Italiano; ArgentinaFil: Nannini, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Colonna, Mariana. Inmunova; ArgentinaFil: Sanguineti, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Inmunova; ArgentinaFil: Zylberman, Vanesa. Inmunova; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Muñoz, Luciana. Inmunova; ArgentinaFil: Dobarro, Martín. Sanatorio Sagrado Corazón; ArgentinaFil: Lebersztein, Gabriel. Sanatorio Sagrado Corazón; ArgentinaFil: Farina, Javier. Gobierno de la Provincia de Buenos Aires. Hospital de Alta Complejidad Cuenca Alta Doctor Nestor Carlos Kirchner.; ArgentinaFil: Vidiella, Gabriela. Sanatorio Agote. Dr. Luis Agote; ArgentinaFil: Bertetti, Anselmo. Sanatorio Guemes Sociedad Anonima.; ArgentinaFil: Crudo, Favio. Universidad Nacional de San Antonio de Areco; ArgentinaFil: Alzogaray, Maria Fernanda. Instituto Medico Platense.; ArgentinaFil: Barcelona, Laura. Municipalidad de Vicente Lopez (buenos Aires). Hospital Municipal Doctor Bernardo Houssay.; ArgentinaFil: Teijeiro, Ricardo. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Doctor Ignacio Pirovano; ArgentinaFil: Lambert, Sandra. Provincia de Buenos Aires. Ministerio de Salud. Hospital Alta Complejidad en Red El Cruce Dr. Néstor Carlos Kirchner Samic; ArgentinaFil: Scublinsky, Darío. Clinica Zabala.; ArgentinaFil: Iacono, Marisa. Provincia del Neuquen. Hospital Provincial Neuquen "dr. E. Castro Rendon"; ArgentinaFil: Stanek, Vanina. Hospital Italiano; ArgentinaFil: Solari, Rubén. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; ArgentinaFil: Cruz, Pablo. No especifíca;Fil: Casas, Marcelo Martín. Clinica Adventista Belgrano; ArgentinaFil: Abusamra, Lorena. Hospital Municipal Dr. Diego Thompson; ArgentinaFil: Luciardi, Héctor Lucas. Provincia de Tucuman. Ministerio de Salud. Sistema Provincial de Salud. Hosp. Centro de Salud "zenon Santillan"; ArgentinaFil: Cremona, Alberto. Hospital Italiano de La Plata; ArgentinaFil: Caruso, Diego. Hospital Español; ArgentinaFil: de Miguel, Bernardo. No especifíca;Fil: Perez Lloret, Santiago. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Universidad Abierta Interamericana. Secretaría de Investigación. Centro de Altos Estudios En Ciencias Humanas y de la Salud - Sede Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Millán, Susana. No especifíca;Fil: Kilstein, Yael. No especifíca;Fil: Pereiro, Ana. Fundación Mundo Sano; ArgentinaFil: Sued, Omar. Fundación Huésped; ArgentinaFil: Cahn, Pedro. Fundación Huésped; ArgentinaFil: Spatz, Linus. Inmunova; ArgentinaFil: Goldbaum, Fernando Alberto. Inmunova; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad Nacional de San Martin. Centro de Rediseño E Ingenieria de Proteinas.; Argentin

Prospective validation of the CLIP score: a new prognostic system for patient with cirrhosis and hepatocellular carcinoma

Prognosis of patients with cirrhosis and hepatocellular carcinoma (HCC) depends on both residual liver function and tumor extension. The CLIP score includes Child-Pugh stage, tumor morphology and extension, serum alfa-fetoprotein (AFP) levels, and portal vein thrombosis. We externally validated the CLIP score and compared its discriminatory ability and predictive power with that of the Okuda staging system in 196 patients with cirrhosis and HCC prospectively enrolled in a randomized trial. No significant associations were found between the CLIP score and the age, sex, and pattern of viral infection. There was a strong correlation between the CLIP score and the Okuda stage, As of June 1999, 150 patients (76.5%) had died. Median survival time was 11 months, overall, and it was 36, 22, 9, 7, and 3 months for CLIP categories 0, 1, 2, 3, and 4 to 6, respectively. In multivariate analysis, the CLIP score had additional explanatory power above that of the Okuda stage. This was true for both patients treated with locoregional therapy or not. A quantitative estimation of 2-year survival predictive power showed that the CLIP score explained 37% of survival variability, compared with 21% explained by Okuda stage. In conclusion, the CLIP score, compared with the Okuda staging system, gives more accurate prognostic information, is statistically more efficient, and has a greater survival predictive power. It could be useful in treatment planning by improving baseline prognostic evaluation of patients with RCC, and could be used in prospective therapeutic trials as a stratification variable, reducing the variability of results owing to patient selection

Defining Kawasaki disease and pediatric inflammatory multisystem syndrome-temporally associated to SARS-CoV-2 infection during SARS-CoV-2 epidemic in Italy: results from a national, multicenter survey

Background: There is mounting evidence on the existence of a Pediatric Inflammatory Multisystem Syndrome-temporally associated to SARS-CoV-2 infection (PIMS-TS), sharing similarities with Kawasaki Disease (KD). The main outcome of the study were to better characterize the clinical features and the treatment response of PIMS-TS and to explore its relationship with KD determining whether KD and PIMS are two distinct entities. Methods: The Rheumatology Study Group of the Italian Pediatric Society launched a survey to enroll patients diagnosed with KD (Kawasaki Disease Group - KDG) or KD-like (Kawacovid Group - KCG) disease between February 1st 2020, and May 31st 2020. Demographic, clinical, laboratory data, treatment information, and patients' outcome were collected in an online anonymized database (RedCAP®). Relationship between clinical presentation and SARS-CoV-2 infection was also taken into account. Moreover, clinical characteristics of KDG during SARS-CoV-2 epidemic (KDG-CoV2) were compared to Kawasaki Disease patients (KDG-Historical) seen in three different Italian tertiary pediatric hospitals (Institute for Maternal and Child Health, IRCCS "Burlo Garofolo", Trieste; AOU Meyer, Florence; IRCCS Istituto Giannina Gaslini, Genoa) from January 1st 2000 to December 31st 2019. Chi square test or exact Fisher test and non-parametric Wilcoxon Mann-Whitney test were used to study differences between two groups. Results: One-hundred-forty-nine cases were enrolled, (96 KDG and 53 KCG). KCG children were significantly older and presented more frequently from gastrointestinal and respiratory involvement. Cardiac involvement was more common in KCG, with 60,4% of patients with myocarditis. 37,8% of patients among KCG presented hypotension/non-cardiogenic shock. Coronary artery abnormalities (CAA) were more common in the KDG. The risk of ICU admission were higher in KCG. Lymphopenia, higher CRP levels, elevated ferritin and troponin-T characterized KCG. KDG received more frequently immunoglobulins (IVIG) and acetylsalicylic acid (ASA) (81,3% vs 66%; p = 0.04 and 71,9% vs 43,4%; p = 0.001 respectively) as KCG more often received glucocorticoids (56,6% vs 14,6%; p < 0.0001). SARS-CoV-2 assay more often resulted positive in KCG than in KDG (75,5% vs 20%; p < 0.0001). Short-term follow data showed minor complications. Comparing KDG with a KD-Historical Italian cohort (598 patients), no statistical difference was found in terms of clinical manifestations and laboratory data. Conclusion: Our study suggests that SARS-CoV-2 infection might determine two distinct inflammatory diseases in children: KD and PIMS-TS. Older age at onset and clinical peculiarities like the occurrence of myocarditis characterize this multi-inflammatory syndrome. Our patients had an optimal response to treatments and a good outcome, with few complications and no deaths

Re-colonising the Southern Alpine fringe: diachronic data on the use of sheltered space in the Late Epigravettian site of Riparo Tagliente (Verona, Italy)

This paper focuses on the use of space by Late Epigravettian groups in the Italian peninsula through the analysis of data from the site of Riparo Tagliente, which has yielded the earliest evidence of re-occupation of the Southern Alpine fringe after the end of the LGM. Previous works had underlined the presence of a persistent pattern in the differential use of the outer area of the site – mostly characterized by secondary refuse accumulations – compared to the sheltered area- dedicated to domestic activities undertaken around hearths. Here a “site-structural” approach has been applied to the abundant evidence of the sheltered area, where an articulated stratigraphic series documenting the first occupation phases in the site – disturbed at the top by an artificial “cut” carried out in historical times – was excavated starting from the late ‘70s of the last century. Radiocarbon dating of this series indicates a chronological span between 17,219 and 15,940 years cal BP and locates these occupations in the first part of the Late Glacial (GS-2.1a). Four phases have thus been recognized, which have then been grouped into two main macro-phases, each of which includes thick habitation soils, hearth-pits and cumulative features (formed by the amassing of different categories of residues). A large sunken “dwelling structural complex” was also identified and attributed to the most recent macro-phase. The two macro-phases record an important change in the organisation of domestic space over time, testified by the displacement of hearth-pits, the different intensity in their use and the variations in the frequencies of the typologies of lithic artefacts. Such modifications could be related to the different social identity of the groups that occupied the site over time or to changes in the duration and/or aims of the occupations possibly connected to settlement dynamics on a wider scale