Ruthenation of non-stacked guanines in DNA G-quadruplex structures : enhancement of c-MYC expression

Guanine quadruplexes (GQs) are compact four-stranded DNA structures that play a key role in the control of a variety of biological processes, including gene transcription. Bulky ruthenium complexes featuring a bipyridine, a terpyridine, and one exchangeable ligand ([Ru(terpy)(bpy)X]n+) are able to metalate exposed guanines present in the GQ of the c-MYC promoter region that are not involved in quadruplex base pairing. qRT-PCR and western-blot experiments indicated that the complexes promote a remarkable increase in the expression of this oncogene. We also show that exchangeable thioether ligands (X=RSR′, Met) allow regulation of the metalating activity of the complex with visible light

El pistachero II: Estudio fenológico y económico

10 páginas, tablas estadísticas y figuras.El pistachero es capaz de sobrevivir y dar frutos en situaciones adversas, intolerables para la mayor parte de los frutales. Sin embargo también tiene unos condicionamientos de medio bastantes específicos que limitan su posible área de cultivo, como son las necesidades en frío para cubrir adecuadamente su periodo de reposos invernal, la sincronía en la floración de machos y hembras para tener una buena polinización y sensibilidad a las heladas tardías de primavera. Con los resultados obtenidos en este trabajo y en el trabajo presentado en este VI Congreso de la SEAE “El pistachero I: Estudio de variedades en secano y en manejo ecológico”, se realiza un estudio de viabilidad económica. Se recomienda que a la hora de hacer una nueva plantación, se incluya en la misma diferentes variedades hembras y machos. Las variedades hembras con las que se han obtenido mejores resultados son: Avdat, Ashoury y Larnaka, y como polinizadores: C-especial, Askar, Nazar, Chico y Mateur. Las variedades recomendadas, tienen unas necesidades cercanas a las 1.000 horas-frío para el inicio de la actividad vegetativa y la floración es en la primera quincena de abril. En el estudio económico y considerando que sólo uno de cada tres años se obtiene producción, debido a problemas de heladas de primavera o por veceria, se pueden obtener una rentabilidad económica, a partir de los veinte años, superior a los 600 €, lo que le permite ser una buena alternativa para algunas zonas del secano españolPeer reviewe

Intracellular deprotection reactions mediated by palladium complexes equipped with designed phosphine ligands

Discrete palladium(II) complexes featuring purposely designed phosphine ligands can promote depropargylation and deallylation reactions in cell lysates. These complexes perform better than other palladium sources, which apparently are rapidly deactivated in such hostile complex media. This good balance between reactivity and stability allows the use of these discrete phosphine palladium complexes in living mammalian cells, whereby they can mediate similar transformations. The presence of a phosphine ligand in the coordination sphere of palladium also provides for the introduction of targeting groups, such as hydrophobic phosphonium moieties, which facilitate the accumulation of the complexes in mitochondria

Anion Recognition as a Supramolecular Switch of Cell Internalization

The cell internalization of designed oligoarginine peptides equipped with six glutamic acid residues and an anionic pyranine at the N-terminus is triggered upon addition of a supramolecular host. This host binds specifically to the pyranine moiety, enabling the complex to traverse the cell membrane. Interestingly, none of the components, neither the host nor the guest, are able to cross the cell membrane on their ownWe are thankful for the support given by the Spanish grants SAF2013-41943-R, CTQ2015-70698-R, and CTQ2013-49317-EXP, the Consellerı́a de Cultura, Educación e Ordenación Universitaria (GRC2013-041 and Centro singular de investigación de Galicia accreditation 2016-2019, ED431G/09), the European Regional Development Fund (ERDF), and the European Research Council (Advanced Grant No. 340055). Support of the orfeo-cinqa network (CTQ2014-51912-REDC) is kindly acknowledged. J.R. thanks the Xunta de Galicia for her Ph.D. fellowship, and J.M. thanks Fundación Ramón Areces for his postdoctoral fellowshipS

The genetic susceptibility linking preterm birth and periodontal disease : a review

Abstract in proceedings of the Fourth International Congress of CiiEM: Health, Well-Being and Ageing in the 21st Century, held at Egas Moniz’ University Campus in Monte de Caparica, Almada, from 3–5 June 2019.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.info:eu-repo/semantics/publishedVersio

The C-terminal SH3 domain contributes to the intramolecular inhibition of Vav family proteins

Vav proteins are phosphorylation-dependent guanine nucleotide exchange factors (GEFs) that catalyze the activation of members of the Rho family of guanosine triphosphatases (GTPases). The current regulatory model holds that the nonphosphorylated, catalytically inactive state of these GEFs is maintained by intramolecular interactions among the amino-terminal domains and the central catalytic core, which block the binding of Vav proteins to GTPases. We showed that this autoinhibition is mechanistically more complex, also involving the bivalent association of the carboxyl-terminal Src homology 3 (SH3) region of Vav with its catalytic and pleckstrin homology (PH) domains. Such interactions occurred through proline-rich region-independent mechanisms. Full release from this double-locked state required synergistic weakening effects from multiple phosphorylated tyrosine residues, thus providing an optimized system to generate gradients of Vav GEF activity depending on upstream signaling inputs. This mechanism is shared by mammalian and Drosophila melanogaster Vav proteins, suggesting that it may be a common regulatory feature for this protein family.Work in the laboratory of X.R.B. has been funded by grants from the Spanish Ministry of Economy and Competitiveness (SAF2009-07172, SAF2012-3171, RD06/0020/0001, and RD12/0036/0002), the Castilla-León Autonomous Government (CSI039A12-1), and the Asociación Española Contra el Cáncer (AECC). O.L. has been supported by grants from the Spanish Ministry of Economy and Competitiveness (SAF2011-22988 and RD06/0020/1001). The salary of M.B. has been partially supported by a JAE-Predoc contract (CSIC), the AECC, and grant RD06/0020/0001. S.F. is supported by a graduate student FPI contract from the Spanish Ministry of Economy and Competitiveness (BES-2010-031386). Spanish funding is cosponsored by the European Regional Development Fund.Peer Reviewe

Anion Recognition as a Supramolecular Switch of Cell Internalization.

The cell internalization of designed oligoarginine peptides equipped with six glutamic acid residues and an anionic pyranine at the N-terminus is triggered upon addition of a supramolecular host. This host binds specifically to the pyranine moiety, enabling the complex to traverse the cell membrane. Interestingly, none of the components, neither the host nor the guest, are able to cross the cell membrane on their own