A New Swiss Federal Act on Gambling: From Missed Opportunities towards a Public Health Approach?

In January 2019, a new Swiss Federal Act on Gambling (Loi federal de jeux d'argent: LJAr) entered into force following a vote by the Swiss electorate. Intended to modernize and harmonize previous law and open the market for online casinos; the new regulations have highlighted the need for a comprehensive monitoring system. The present article outlines work undertaken by experts within the field to identify and elaborate the first steps towards developing such a monitoring system. This work includes the mapping of institutional actors and draft conceptualization of an impact model, including structural (i.e., prevention and intervention-based components), process (means), and outcomes (effect) indicators. Initial estimations of effective access to indicators and their perceived priority for data gathering are also described. Subsequent steps necessary for implementation of this public health approach for gambling are considered including grey areas for future action

Additional amphivasal bundles in pedicel pith exacerbate central fruit dominance and induce self-thinning of lateral fruitlets in apple

Apple (Malus x domestica) trees naturally produce an excess of fruitlets that negatively affect the commercial value of fruits brought to maturity, and impact their capacity to develop flower buds the following season. Therefore, chemical thinning has become an important cultural practice allowing the selective removal of unwanted fruitlets. As the public pressure to limit the use of chemical agents increases, the control of thinning becomes a major issue. Here, we characterized the self-thinning capacity of an apple hybrid-genotype, from a tree scale to a molecular level. Additional amphivasal vascular bundles were identified in the pith of pedicels supporting the fruitlets with the lowest abscission potential (central fruitlet), indicating that these bundles might have a role in the acquisition of dominance over lateral fruitlets. Sugar content analysis revealed that central fruitlets were better supplied in sorbitol than laterals\u27. Transcriptomic profiles allowed us to identify genes potentially involved in the over-production of vascular tissues in central pedicels. In addition, histological and transcriptomic data permitted a detailed characterization of abscission zone (AZ) development and the identification of key genes involved in this process. Our data confirm the major role of ethylene, auxin, and cell wall remodeling enzymes in AZ formation. The shedding process in this hybrid appears to be triggered by a naturally exacerbated dominance of central fruitlets over lateral ones, brought about by an increased supply of sugars, possibly through additional amphivasal vascular bundles. The characterization of this genotype opens new perspectives for the selection of elite apple cultivars

Role of miR-2392 in driving SARS-CoV-2 infection

MicroRNAs (miRNAs) are small non-coding RNAs involved in post-transcriptional gene regulation that have a major impact on many diseases and provide an exciting avenue toward antiviral therapeutics. From patient transcriptomic data, we determined that a circulating miRNA, miR-2392, is directly involved with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) machinery during host infection. Specifically, we show that miR-2392 is key in driving downstream suppression of mitochondrial gene expression, increasing inflammation, glycolysis, and hypoxia, as well as promoting many symptoms associated with coronavirus disease 2019 (COVID-19) infection. We demonstrate that miR-2392 is present in the blood and urine of patients positive for COVID-19 but is not present in patients negative for COVID-19. These findings indicate the potential for developing a minimally invasive COVID-19 detection method. Lastly, using in vitro human and in vivo hamster models, we design a miRNA-based antiviral therapeutic that targets miR-2392, significantly reduces SARS-CoV-2 viability in hamsters, and may potentially inhibit a COVID-19 disease state in humans

Modélisation de la croissance et de la ramification chez quelques cultivars de pommier

National audienc

Deciphering the genetic determinism of bud phenology in apple progenies: a new insight into chilling and heat requirement effects on flowering dates and positional candidate genes

The present study investigates the genetic determinism of bud phenological traits using two segregating F1 apple (Malus x domestica) progenies. Phenological trait variability was dissected into genetic and climatic components using mixed linear modeling, and estimated best linear unbiased predictors were used for quantitative trait locus (QTL) detection. For flowering dates, year effects were decomposed into chilling and heat requirements based on a previously developed model. QTL analysis permitted the identification of two major and population-specific genomic regions on LG08 and LG09. Both ‘chilling requirement’ and ‘heat requirement’ periods influenced flowering dates, although their relative impact was dependent on the genetic background. Using the apple genome sequence data, putative candidate genes underlying one major QTL were investigated. Numerous key genes involved in cell cycle control were identified in clusters within the confidence interval of the major QTL on LG09. Our results contribute towards a better understanding of the interaction between QTLs and climatic conditions, and provide a basis for the identification of genes involved in bud growth resumption

Comparison of apricot tree growth and development in 3 french growing areas

National audienc

Spatiotemporal characterization of ionizing radiation induced DNA damage foci and their relation to chromatin organization

DNA damage sensing proteins have been shown to localize to the sites of DNA double strand breaks (DSB) within seconds to minutes following ionizing radiation (IR) exposure, resulting in the formation of microscopically visible nuclear domains referred to as radiation-induced foci (RIF). This review characterizes the spatiotemporal properties of RIF at physiological doses, minutes to hours following exposure to ionizing radiation, and it proposes a model describing RIF formation and resolution as a function of radiation quality and chromatin territories. Discussion is limited to RIF formed by three interrelated proteins ATM (Ataxia telangiectasia mutated), 53BP1 (p53 binding protein 1) and γH2AX (phosphorylated variant histone H2AX), with an emphasis on the later. This review discusses the importance of not equating RIF with DSB in all situations and shows how dose and time dependence of RIF frequency is inconsistent with a one to one equivalence. Instead, we propose that RIF mark regions of the chromatin that would serve as scaffolds rigid enough to keep broken DNA from diffusing away, but open enough to allow the repair machinery to access the damage site. We review data indicating clear kinetic and physical differences between RIF emerging from dense and uncondensed regions of the nucleus. We suggest that persistent RIF observed days following exposure to ionizing radiation are nuclear marks of permanent rearrangement of the chromatin architecture. Such chromatin alterations may not always lead to growth arrest as cells have been shown to replicate these in progeny. Thus, heritable persistent RIF spanning over tens of Mbp may reflect persistent changes in the transcriptome of a large progeny of cells. Such model opens the door to a “non-DNA-centric view” of radiation-induced phenotypes