Propriedades físicas e químicas do solo em áreas com sistemas produtivos e mata na região da Zona da Mata mineira.

Tendo em vista o potencial de produção e a extensão de área que ocupam os Latossolos, é de grande interesse estabelecer sistemas de manejo que não modifiquem o solo de forma a comprometer sua sustentabilidade. Nesse sentido, avaliaram-se as alterações em propriedades físicas e químicas de um Latossolo sob diferentes sistemas agrícolas na Zona da Mata mineira. Foram avaliadas áreas sob laranja, cana, pastagem e mata: as de laranja e pastagem têm mais de 20 anos de uso; a de cana é anterior (100 anos). A cana é remanescente de uso histórico por aproximadamente 120 anos. A amostragem foi realizada em trincheiras, nas profundidades de 0,0 a 0,2 e 0,2 a 0,4 m. Foram analisadas as características químicas: matéria orgânica do solo (MOS), pH em H2O, P disponível, Ca2+, Mg2+, K+, Al3+, H + Al, soma de bases (SB), CTC efetiva e total (t) e saturação por bases (V), e P-remanescente. As características físicas analisadas foram: textura, densidade de partículas, densidade do solo, porosidade total, curva de retenção de água, resistência do solo à penetração e estabilidade de agregados em água, bem como a relação capacidade de campo/porosidade total (CC/PT). Para análise dos dados, consideraram-se os usos agrícolas como tratamentos, em um delineamento inteiramente casualizado, na análise de variância de cada profundidade separadamente. Os sistemas agrícolas com laranjeira e canavial melhoraram a condição química do solo, aumentando os teores de nutrientes e diminuindo o Al3+ do complexo de troca, porém apresentaram redução dos teores de MOS e níveis intermediários de degradação física. O sistema com pastagem apresentou baixa fertilidade e caráter distrófico, redução acentuada do teor de MOS e degradação física mais avançada, evidenciada pelo aumento da densidade do solo e resistência à penetração, bem como redução da porosidade total e da estabilidade de agregados

Silages of agro-industrial by-products in lamb diets : effct on growth performance, carcass, meat quality and in vitro methane emissions

Research Areas: AgricultureABSTRACT - The use of agro-industrial by-products in animal feed is an opportunity to reduce imports, food waste and promote a clean and circular economy, turning worthless raw materials into high -quality and low-cost animal feeds, which does not compete with human food. This study aims to produce silages with by-products of carrot, sweet potato, potato, and tomato pomace and integrate them in lamb diets, replacing 50% of dry matter (DM) of a concentrate-based diet. Effects on growth performance, meat quality and methane production were evaluated. Three silages were produced using 350 g/kg tomato pomace, 200 g/kg wheat bran, 150 g/kg alfalfa hay and 300 g/kg potato (Psil) or 300 g/kg sweet potato (SPsil) or 300 g/kg carrot (Csil). Thirty-two lambs were housed individually and divided into four groups (8 animals/group) on the following diets: Control -850 g/kg concentrate and 150 g/kg hay; P -500 g/kg concentrate and 500 g/kg Psil in DM; SP - 500 g/kg concentrate and 500 g/kg SPsil in DM; C - 500 g/kg concentrate and 500 g/kg Csil in DM. The trial lasted 6 weeks after 1 week of adaptation. Methane production was assessed in vitro by the Ankom system, using as inoculum rumen content samples collected individually from 6 lambs on each diet at slaughter. Diet had no effect on DM intake, average daily gain and DM conversion ratio, averaging 1055 +/- 248 g, 320 +/- 61.1 g and 3.35 +/- 0.600, respectively. Also, carcass characteristics were not affected by the diet except for the lightness of subcutaneous fat which was increased by the silage diets (L* value, P = 0.016). The meat pa-rameters, pH, intramuscular fat, shear force, cooking losses and sensory attributes, were not affected by the diet. In meat color, the parameters a* and Chroma were highest in animals fed diet C, have an intermediate value with Control diet and are lowest with diets P and SP (P = 0.027 and P = 0.012, respectively). Diets had no impact on total methane emissions. The costs of the silage diets per kg live weight gain were lower than those of Control diet (P = 0.018). In summary, by-product silage can be a good option to replace concentrated feed in lamb diets without altering the growth performance and meat quality or methane production and thus contributing to the sustainability of livestock farms and the environment.info:eu-repo/semantics/publishedVersio

Outdoor play as a mean to achieve educational goals: a case study in a Portuguese day-care group

This study aims to explore the pedagogical potential of outdoor play situations, considering the educational goals established in the Portuguese Pedagogical Guidelines for 0–3-year-old children (ongoing work), namely development of a sense of security and positive selfesteem; development of curiosity and exploratory impetus; and development of social and communication skills. Following a qualitative methodology, during a period of 9 months, the outdoor play experiences of 14 children from a day-care centre were observed and monitored and the professionals were interviewed. The data shows that outdoor experiences can contribute to the achievement of the educational goals, enlarging opportunities for creative play, problem solving, experimentation, inquiry, dialogues and collaboration. The achievement of the educational goals is facilitated through supportive and positive relationships with adults and peers, provision for proper equipment, and contact with nature. This study offers a deep insight into how educational policies can be put into practice in the outdoor area.publishe

The Second Transmembrane Domain of P2X7 Contributes to Dilated Pore Formation

Activation of the purinergic receptor P2X7 leads to the cellular permeability of low molecular weight cations. To determine which domains of P2X7 are necessary for this permeability, we exchanged either the C-terminus or portions of the second transmembrane domain (TM2) with those in P2X1 or P2X4. Replacement of the C-terminus of P2X7 with either P2X1 or P2X4 prevented surface expression of the chimeric receptor. Similarly, chimeric P2X7 containing TM2 from P2X1 or P2X4 had reduced surface expression and no permeability to cationic dyes. Exchanging the N-terminal 10 residues or C-terminal 14 residues of the P2X7 TM2 with the corresponding region of P2X1 TM2 partially restored surface expression and limited pore permeability. To further probe TM2 structure, we replaced single residues in P2X7 TM2 with those in P2X1 or P2X4. We identified multiple substitutions that drastically changed pore permeability without altering surface expression. Three substitutions (Q332P, Y336T, and Y343L) individually reduced pore formation as indicated by decreased dye uptake and also reduced membrane blebbing in response to ATP exposure. Three others substitutions, V335T, S342G, and S342A each enhanced dye uptake, membrane blebbing and cell death. Our results demonstrate a critical role for the TM2 domain of P2X7 in receptor function, and provide a structural basis for differences between purinergic receptors. © 2013 Sun et al

The cognitive-affective-conative model of destination image: a confirmatory analysis

Destination image influences tourist behaviour before, during and after travel, as it is an important instrument which contributes to tourists’ loyalty. Although Gartner (1993) advocates that the cognitive, affective and conative dimensions of destination image are hierarchically interrelated, there is no empirical evidence to support the complete model. This study aims to test the hierarchical nature of the relationship between the dimensions of destination image. The results of structural equation modelling confirm Gartner’s theoretical model, validating the theory that the influence of the cognitive component on the conative dimension is higher when mediated by the affective component, raising managerial implications

Contribution of Efflux to the Emergence of Isoniazid and Multidrug Resistance in Mycobacterium tuberculosis

Multidrug resistant (MDR) tuberculosis is caused by Mycobacterium tuberculosis resistant to isoniazid and rifampicin, the two most effective drugs used in tuberculosis therapy. Here, we investigated the mechanism by which resistance towards isoniazid develops and how overexpression of efflux pumps favors accumulation of mutations in isoniazid targets, thus establishing a MDR phenotype. The study was based on the in vitro induction of an isoniazid resistant phenotype by prolonged serial exposure of M. tuberculosis strains to the critical concentration of isoniazid employed for determination of drug susceptibility testing in clinical isolates. Results show that susceptible and rifampicin monoresistant strains exposed to this concentration become resistant to isoniazid after three weeks; and that resistance observed for the majority of these strains could be reduced by means of efflux pumps inhibitors. RT-qPCR assessment of efflux pump genes expression showed overexpression of all tested genes. Enhanced real-time efflux of ethidium bromide, a common efflux pump substrate, was also observed, showing a clear relation between overexpression of the genes and increased efflux pump function. Further exposure to isoniazid resulted in the selection and stabilization of spontaneous mutations and deletions in the katG gene along with sustained increased efflux activity. Together, results demonstrate the relevance of efflux pumps as one of the factors of isoniazid resistance in M. tuberculosis. These results support the hypothesis that activity of efflux pumps allows the maintenance of an isoniazid resistant population in a sub-optimally treated patient from which isoniazid genetically resistant mutants emerge. Therefore, the use of inhibitors of efflux should be considered in the development of new therapeutic strategies for preventing the emergence of MDR-TB during treatment