Tuberculosis in roe deer from Spain and Italy

TUBERCULOSIS (TB) is a chronic infectious disease caused by bacteria of the genus Mycobacterium (Grange and others 1990). The detection of wildlife reservoirs of disease is important, particularly in areas where there is a relatively low incidence of the disease in domestic animals. Tuberculosis cases in roe deer (Capreolus capreolus) are reported only sporadically, despite the wide distribution and the abundance of this cervid. Roe deer with TB have been reported in Germany (Schmidt 1938), Switzerland (Bouvier 1963), France (Zanella and others 2008) and the UK (Gunning 1985, Delahay and others 2007). This short communication is the first report of TB in roe deer in Spain and Italy, and discusses the implications of these findings for wildlife and livestock disease control. The prevalence of mycobacterial infections, such as TB and paratuberculosis, seems to be increasing in Spain. Wildlife species may act as disease reservoirs, so this short communication also elucidates the epidemiology of mycobacterial infections in species such as roe deer

About curvature, conformal metrics and warped products

We consider the curvature of a family of warped products of two pseduo-Riemannian manifolds $(B,g_B)$ and $(F,g_F)$ furnished with metrics of the form $c^{2}g_B \oplus w^2 g_F$ and, in particular, of the type $w^{2 \mu}g_B \oplus w^2 g_F$, where $c, w \colon B \to (0,\infty)$ are smooth functions and $\mu$ is a real parameter. We obtain suitable expressions for the Ricci tensor and scalar curvature of such products that allow us to establish results about the existence of Einstein or constant scalar curvature structures in these categories. If $(B,g_B)$ is Riemannian, the latter question involves nonlinear elliptic partial differential equations with concave-convex nonlinearities and singular partial differential equations of the Lichnerowicz-York type among others.Comment: 32 pages, 3 figure

The contribution of Real Madrid’s first five European Cups to the emergence of a common football space

Real Madrid won the first five editions of the European Champion Clubs’ Cup (now formally known as the UEFA Champions League, and to which we will refer hereon as the European Cup) between 1956 and 1960, contributing decisively to the competition’s consolidation. The tournament’s history started towards the end of 1954, when a journalist of the French sports daily L’Équipe, Gabriel Hanot, published an article arguing the need to organise a competition that could bring together the champions of every European league. However, this was not an original proposal. Thirty years before Hanot’s article proposals for such a football competition were circulated among the game’s ruling elite. Unfortunately, at that time the lack of a good transport infrastructure to travel through Europe discouraged the proposers which, instead, turned their attention to regional supranational competitions, such as the Mitropa Cup or the Latin Cup. The first five editions of the European Cup witnessed as many victories of Real Madrid, thus forging an indissoluble bond between the competition and the Spanish club. These five European titles did not only cement the supremacy of Real Madrid on the pitch as a great football team, but they also contributed to the consolidation of the European Cup itself in the public’s imaginary. We also argue that given the expectations raised by Real Madrid’s triumphs across Europe those matches might have contributed as well to the emergence of a European football space. Since 1955 Real Madrid occupied an ever increasing space in the press across Europe. Real Madrid was then considered as the best expression of modernity in football. This chapter aims to analyse the meaning of these five European Cup titles for the emergence and definition of a nascent European football space. We, of course, also question whether such a common space can be found. The chapter explores in depth the reasons behind Real Madrid’s enthusiasm with the new European competition. We also examine the social impact that Real Madrid’s hegemony in the European Cup had in the context of Spain’s international isolation during General Franco’s dictatorship (1939-1975). In order to achieve the above mentioned objectives, the chapter relies on thematic analysis of selected publications in the Spanish and British press during those years. Moreover, we have also relied on a review of academic literature on the role of Real Madrid during the Franco dictatorship years, mainly the 1950s and 60s. This chapter is part of wider on-going research. In this research we examine the content of two Spanish dailies (ABC and Marca) and three British newspapers (The Guardian, The Times and the Daily Mirror). We searched these newspapers for content related to Real Madrid on specific dates: The semifinal games (two legs) and the final of each one of the five years where Real Madrid won the European Cup. We searched for content the day of each match, the day before and two days after each one of the matches. This chapter is a presentation of the findings obtained through thematic analysis of the data obtained through those searches

The materiality of the intangible: Literary metaphor in multimodal texts

Based on a larger practice-based research project in digital writing, this article examines how the materiality of digital media contributes to a layered metaphor that delivers meaning, reflects on the cognitive processes (the writer’s and the reader’s) of navigation and generates a dynamic narrative structure through multimodality, unnatural narration and user interaction. Many writers and artists engage with their chosen medium through an instinctive understanding of the materials at hand, gained through experience; the explicit study of a medium’s materiality is not always required for artistic success, however, that may be judged. This article offers insights into the creative process of creating digital, multimodal fiction, based on a practice-based research project designed to explore the effects of digital media on author and text, and argues that digital media have a significant effect on the outcome of the artefact itself. Awareness of these effects, their variations according to hardware and software, and the affordances of these various materials offer the digital writer greater insight and capability to craft his/her texts for the desired metaphorical meaning

Small Bowel Enteroscopy - A Joint Clinical Guideline by the Spanish and Portuguese Small-Bowel Study Groups

The present evidence-based guidelines are focused on the use of device-assisted enteroscopy in the management of small-bowel diseases. A panel of experts selected by the Spanish and Portuguese small-bowel study groups reviewed the available evidence focusing on the main indications of this technique, its role in the management algorithm of each indication, and its diagnostic and therapeutic yield. A set of recommendations was issued accordingly.info:eu-repo/semantics/publishedVersio

Fusion between Leishmania amazonensis and Leishmania major Parasitophorous Vacuoles: Live Imaging of Coinfected Macrophages

Protozoan parasites of the genus Leishmania alternate between flagellated, elongated extracellular promastigotes found in insect vectors, and round-shaped amastigotes enclosed in phagolysosome-like Parasitophorous Vacuoles (PVs) of infected mammalian host cells. Leishmania amazonensis amastigotes occupy large PVs which may contain many parasites; in contrast, single amastigotes of Leishmania major lodge in small, tight PVs, which undergo fission as parasites divide. To determine if PVs of these Leishmania species can fuse with each other, mouse macrophages in culture were infected with non-fluorescent L. amazonensis amastigotes and, 48 h later, superinfected with fluorescent L. major amastigotes or promastigotes. Fusion was investigated by time-lapse image acquisition of living cells and inferred from the colocalization of parasites of the two species in the same PVs. Survival, multiplication and differentiation of parasites that did or did not share the same vacuoles were also investigated. Fusion of PVs containing L. amazonensis and L. major amastigotes was not found. However, PVs containing L. major promastigotes did fuse with pre-established L. amazonensis PVs. In these chimeric vacuoles, L. major promastigotes remained motile and multiplied, but did not differentiate into amastigotes. In contrast, in doubly infected cells, within their own, unfused PVs metacyclic-enriched L. major promastigotes, but not log phase promastigotes - which were destroyed - differentiated into proliferating amastigotes. The results indicate that PVs, presumably customized by L. major amastigotes or promastigotes, differ in their ability to fuse with L. amazonensis PVs. Additionally, a species-specific PV was required for L. major destruction or differentiation – a requirement for which mechanisms remain unknown. The observations reported in this paper should be useful in further studies of the interactions between PVs to different species of Leishmania parasites, and of the mechanisms involved in the recognition and fusion of PVs

The treatment with the probiotic Shewanella putrefaciens Pdp11 of sepecimens of Solea senegalensis exposed to high stocking densities to enhance their resistance to disease

Aquaculture industry exposes fish to acute stress events, such as high stocking density, and a link between stress and higher susceptibility to diseases has been concluded. Several studies have demonstrated increased stress tolerance of fish treated with probiotics, but the mechanisms involved have not been elucidated. Shewanella putrefaciens Pdp11 is a strain isolated from healthy gilthead seabream (Sparus aurata L.) and it is considered as probiotics. The aim of this study was to evaluate the effect of the dietary administration of this probiotics on the stress tolerance of Solea senegalensis specimens farmed under high stocking density (PHD) compared to a group fed a commercial diet and farmed under the same conditions (CHD). In addition, during the experiment, a natural infectious outbreak due to Vibrio species affected fish farmed under crowding conditions. Changes in the microbiota and histology of intestine and in the transcription of immune response genes were evaluated at 19 and 30 days of the experiment. Mortality was observed after 9 days of the beginning of the experiment in CHD and PHD groups, it being higher in the CHD group. Fish farmed under crowding stress showed reduced expression of genes at 19 day probiotic feeding. On the contrary, a significant increase in immune related gene expression was detected in CHD fish at 30 day, whereas the gene expression in fish from PHD group was very similar to that showed in specimens fed and farmed with the conventional conditions. In addition, the dietary administration of S. putrefaciens Pdp11 produced an important modulation of the intestinal microbiota, which was significantly correlated with the high number of goblet cells detected in fish fed the probiotic diet.S

TET family dioxygenases and DNA demethylation in stem cells and cancers

The methylation of cytosine and subsequent oxidation constitutes a fundamental epigenetic modification in mammalian genomes, and its abnormalities are intimately coupled to various pathogenic processes including cancer development. Enzymes of the Ten-eleven translocation (TET) family catalyze the stepwise oxidation of 5-methylcytosine in DNA to 5-hydroxymethylcytosine and further oxidation products. These oxidized 5-methylcytosine derivatives represent intermediates in the reversal of cytosine methylation, and also serve as stable epigenetic modifications that exert distinctive regulatory roles. It is becoming increasingly obvious that TET proteins and their catalytic products are key regulators of embryonic development, stem cell functions and lineage specification. Over the past several years, the function of TET proteins as a barrier between normal and malignant states has been extensively investigated. Dysregulation of TET protein expression or function is commonly observed in a wide range of cancers. Notably, TET loss-of-function is causally related to the onset and progression of hematologic malignancy in vivo. In this review, we focus on recent advances in the mechanistic understanding of DNA methylation-demethylation dynamics, and their potential regulatory functions in cellular differentiation and oncogenic transformation

'Then look!' Unborn attachments and the half-moving image

This article explores the emotional impact on the viewer of disturbing and disorienting images of infant-caregiver relationality in four “melo-horror” films: Imitation of Life (Stahl, 1934), Imitation of Life (Sirk, 1959), The Brood (Cronenberg, 1979), and Beloved (Demme, 1998). Comparing some of these filmic images with the infant performances of “disorganized” attachment styles captured on videotape by attachment researchers such as Mary Main, the author argues that the filmed audiovisual enactment of relational trauma, whether in the context of scientific research or cinematic art, offers the spectator an opportunity to work consciously and unconsciously with representations of unbearable psychic and psychosocial experience—both her own and that of others—that may hitherto have been thought unrepresentable or simply not thought at all