Powertrain modelling for engine stop-start dynamics and control of micro/mild hybrid construction machines

Engine stop-start control is considered as the key technology for micro/mild hybridisation of vehicles and machines. To utilize this concept, especially for construction machines, the engine is desired to be started in such a way that the operator discomfort can be minimized. To address this issue, this paper aims to develop a simple powertrain modelling approach for engine stop-start dynamic analysis and an advanced engine start control scheme newly applicable for micro/mild hybrid construction machines. First, a powertrain model of a generic construction machine is mathematically developed in a general form which allows to investigate the transient responses of the system during the engine cranking process. Second, a simple parameterisation procedure with a minimum set of data required to characterise the dynamic model is presented. Third, a model- based adaptive controller is designed for the starter to crank the engine quickly and smoothly without the need of fuel injection while the critical problems of machine noise, vibration and harshness can be eliminated. Finally, the advantages and effectiveness of the proposed modelling and control approaches have been validated through numerical simulations. The results imply that with the limited data set for training, the developed model works better than a high fidelity model built in AMESim while the adaptive controller can guarantee the desired cranking performance

Challenges of micro/mild hybridisation for construction machinery and applicability in UK

In recent years, micro/mild hybridisation (MMH) is known as a feasible solution for powertrain development with high fuel efficiency, less energy use and emission and, especially, low cost and simple installation. This paper focuses on the challenges of MMH for construction machines and then, pays attention to its applicability to UK construction machinery. First, hybrid electric configurations are briefly reviewed; and technological challenges towards MMH in construction sector are clearly stated. Second, the current development of construction machinery in UK is analysed to point out the potential for MMH implementation. Thousands of machines manufactured in UK have been sampled for the further study. Third, a methodology for big data capturing, compression and mining is provided for a capable of managing and analysing effectively performances of various construction machine types. By using this method, 96% of data memory can be reduced to store the huge machine data without lacking the necessary information. Forth, an advanced decision tool is built using a fuzzy cognitive map based on the big data mining and knowledge from experts to enables users to define a target machine for MMH utilization. The numerical study with this tool on the sampled machines has been done and finally realized that one class of heavy excavators is the most suitable to apply MMH technology

Powertrain modelling and engine start control of construction machines

This paper aims to develop an engine start control approach for a micro/mild hybrid machine for a capable of cranking the engine without injection. First, the powertrain is physically modelled using a co-simulation platform. Second, experiment data of the traditional machine is acquired to optimize the model. Third, a model-based adaptive controller is designed for the starter to crank the engine quickly and smoothly to minimize the operator discomfort. The effectiveness of the proposed approach is validated through numerical simulations with the established model

Novel mutations in human and mouse SCN4A implicate AMPK in myotonia and periodic paralysis

Mutations in the skeletal muscle channel (SCN4A), encoding the Nav1.4 voltage-gated sodium channel, are causative of a variety of muscle channelopathies, including non-dystrophic myotonias and periodic paralysis. The effects of many of these mutations on channel function have been characterized both in vitro and in vivo. However, little is known about the consequences of SCN4A mutations downstream from their impact on the electrophysiology of the Nav1.4 channel. Here we report the discovery of a novel SCN4A mutation (c.1762A>G; p.I588V) in a patient with myotonia and periodic paralysis, located within the S1 segment of the second domain of the Nav1.4 channel. Using N-ethyl-N-nitrosourea mutagenesis, we generated and characterized a mouse model (named draggen), carrying the equivalent point mutation (c.1744A>G; p.I582V) to that found in the patient with periodic paralysis and myotonia. Draggen mice have myotonia and suffer from intermittent hind-limb immobility attacks. In-depth characterization of draggen mice uncovered novel systemic metabolic abnormalities in Scn4a mouse models and provided novel insights into disease mechanisms. We discovered metabolic alterations leading to lean mice, as well as abnormal AMP-activated protein kinase activation, which were associated with the immobility attacks and may provide a novel potential therapeutic target

A Relationship between Carotenoid Accumulation and the Distribution of Species of the Fungus Neurospora in Spain

The ascomycete fungus Neurospora is present in many parts of the world, in particular in tropical and subtropical areas, where it is found growing on recently burned vegetation. We have sampled the Neurospora population across Spain. The sampling sites were located in the region of Galicia (northwestern corner of the Iberian peninsula), the province of Cáceres, the city of Seville, and the two major islands of the Canary Islands archipelago (Tenerife and Gran Canaria, west coast of Africa). The sites covered a latitude interval between 27.88° and 42.74°. We have identified wild-type strains of N. discreta, N. tetrasperma, N. crassa, and N. sitophila and the frequency of each species varied from site to site. It has been shown that after exposure to light Neurospora accumulates the orange carotenoid neurosporaxanthin, presumably for protection from UV radiation. We have found that each Neurospora species accumulates a different amount of carotenoids after exposure to light, but these differences did not correlate with the expression of the carotenogenic genes al-1 or al-2. The accumulation of carotenoids in Neurospora shows a correlation with latitude, as Neurospora strains isolated from lower latitudes accumulate more carotenoids than strains isolated from higher latitudes. Since regions of low latitude receive high UV irradiation we propose that the increased carotenoid accumulation may protect Neurospora from high UV exposure. In support of this hypothesis, we have found that N. crassa, the species that accumulates more carotenoids, is more resistant to UV radiation than N. discreta or N. tetrasperma. The photoprotection provided by carotenoids and the capability to accumulate different amounts of carotenoids may be responsible, at least in part, for the distribution of Neurospora species that we have observed across a range of latitudes

Genomic Analysis of the Basal Lineage Fungus Rhizopus oryzae Reveals a Whole-Genome Duplication

Rhizopus oryzae is the primary cause of mucormycosis, an emerging, life-threatening infection characterized by rapid angioinvasive growth with an overall mortality rate that exceeds 50%. As a representative of the paraphyletic basal group of the fungal kingdom called “zygomycetes,” R. oryzae is also used as a model to study fungal evolution. Here we report the genome sequence of R. oryzae strain 99–880, isolated from a fatal case of mucormycosis. The highly repetitive 45.3 Mb genome assembly contains abundant transposable elements (TEs), comprising approximately 20% of the genome. We predicted 13,895 protein-coding genes not overlapping TEs, many of which are paralogous gene pairs. The order and genomic arrangement of the duplicated gene pairs and their common phylogenetic origin provide evidence for an ancestral whole-genome duplication (WGD) event. The WGD resulted in the duplication of nearly all subunits of the protein complexes associated with respiratory electron transport chains, the V-ATPase, and the ubiquitin–proteasome systems. The WGD, together with recent gene duplications, resulted in the expansion of multiple gene families related to cell growth and signal transduction, as well as secreted aspartic protease and subtilase protein families, which are known fungal virulence factors. The duplication of the ergosterol biosynthetic pathway, especially the major azole target, lanosterol 14α-demethylase (ERG11), could contribute to the variable responses of R. oryzae to different azole drugs, including voriconazole and posaconazole. Expanded families of cell-wall synthesis enzymes, essential for fungal cell integrity but absent in mammalian hosts, reveal potential targets for novel and R. oryzae-specific diagnostic and therapeutic treatments

Recent advances in understanding the roles of whole genome duplications in evolution

Ancient whole-genome duplications (WGDs)—paleopolyploidy events—are key to solving Darwin’s ‘abominable mystery’ of how flowering plants evolved and radiated into a rich variety of species. The vertebrates also emerged from their invertebrate ancestors via two WGDs, and genomes of diverse gymnosperm trees, unicellular eukaryotes, invertebrates, fishes, amphibians and even a rodent carry evidence of lineage-specific WGDs. Modern polyploidy is common in eukaryotes, and it can be induced, enabling mechanisms and short-term cost-benefit assessments of polyploidy to be studied experimentally. However, the ancient WGDs can be reconstructed only by comparative genomics: these studies are difficult because the DNA duplicates have been through tens or hundreds of millions of years of gene losses, mutations, and chromosomal rearrangements that culminate in resolution of the polyploid genomes back into diploid ones (rediploidisation). Intriguing asymmetries in patterns of post-WGD gene loss and retention between duplicated sets of chromosomes have been discovered recently, and elaborations of signal transduction systems are lasting legacies from several WGDs. The data imply that simpler signalling pathways in the pre-WGD ancestors were converted via WGDs into multi-stranded parallelised networks. Genetic and biochemical studies in plants, yeasts and vertebrates suggest a paradigm in which different combinations of sister paralogues in the post-WGD regulatory networks are co-regulated under different conditions. In principle, such networks can respond to a wide array of environmental, sensory and hormonal stimuli and integrate them to generate phenotypic variety in cell types and behaviours. Patterns are also being discerned in how the post-WGD signalling networks are reconfigured in human cancers and neurological conditions. It is fascinating to unpick how ancient genomic events impact on complexity, variety and disease in modern life

Relevance of the light signaling machinery for cellulase expression in trichoderma reesei (hypocrea jecorina)

<p>Abstract</p> <p>Background</p> <p>In nature, light is one of the most important environmental cues that fungi perceive and interpret. It is known not only to influence growth and conidiation, but also cellulase gene expression. We therefore studied the relevance of the main components of the light perception machinery of <it>Trichoderma reesei </it>(<it>Hypocrea jecorina</it>), ENV1, BLR1 and BLR2, for production of plant cell wall degrading enzymes in fermentations aimed at efficient biosynthesis of enzyme mixtures for biofuel production.</p> <p>Findings</p> <p>Our results indicate that despite cultivation in mostly dark conditions, all three components show an influence on cellulase expression. While we found the performance of the enzyme mixture secreted by a deletion mutant in <it>env1 </it>to be enhanced, the higher cellulolytic activity observed for <it>Δblr2 </it>is mainly due to an increased secretion capacity of this strain. <it>Δblr1 </it>showed enhanced biomass accumulation, but due to its obviously lower secretion capacity still was the least efficient strain in this study.</p> <p>Conclusions</p> <p>We conclude that with respect to regulation of plant cell wall degrading enzymes, the blue light regulator proteins are unlikely to act as a complex. Their regulatory influence on cellulase biosynthesis involves an alteration of protein secretion, which may be due to adjustment of transcription or posttranscriptional regulation of upstream factors. In contrast, the regulatory function of ENV1 seems to involve adjustment of enzyme proportions to environmental conditions.</p

Cenozoic deformation of Iberia: a model for intraplate mountain building and basin development based on analogue modelling

Inferences from analogue models support lithospheric folding as the primary response to large-scale shortening manifested in the present day topography of Iberia. This process was active from the late Oligocene-early Miocene during the Alpine orogeny and was probably enhanced by reactivation of inherited Variscan faults. The modeling results confirm the dependence of fold wavelength on convergence rate and hence the strength of the layers of the lithosphere such that fold wavelength is longest for fast convergence rates favoring whole lithosphere folding. Folding is associated with the formation of dominantly pop-up type mountain ranges in the brittle crust and thickening of the ductile layers in the synforms of the buckle folds by flow. The mountain ranges are represented by upper crustal pop-ups forming the main topographic relief. The wavelengths of the topographic uplifts, both, in model and nature suggest mechanical decoupling between crust and mantle. Moreover, our modeling results suggest that buckling in Iberia took place under rheological conditions where the lithospheric mantle is stronger than the lower crust. The presence of an indenter, inducing oblique shortening in response to the opening of the King's Trough in the north western corner of the Atlantic Iberian margin controls the spacing and obliquity of structures. This leads to the transfer of the deformation from the moving walls towards the inner part of the model, creating oblique structures in both brittle and ductile layers. The effect of the indenter, together with an increase on the convergence rate produced more complex brittle structures. These results show close similarities to observations on the general shape and distribution of mountain ranges and basins in Iberia, including the Spanish Central System and Toledo Mountains.Peer reviewe