21 research outputs found
Rapid fine mapping of causative mutations from sets of unordered, contig-sized fragments of genome sequence
Background Traditional Map based Cloning approaches, used for the identification of desirable alleles, are extremely labour intensive and years can elapse between the mutagenesis and the detection of the polymorphism. High throughput sequencing based Mapping-by-sequencing approach requires an ordered genome assembly and cannot be used with fragmented, un-scaffolded draft genomes, limiting its application to model species and precluding many important organisms. Results We addressed this gap in resource and presented a computational method and software implementations called CHERIPIC (Computing Homozygosity Enriched Regions In genomes to Prioritise Identification of Candidate variants). We have successfully validated implementation of CHERIPIC using three different types of bulk segregant sequence data from Arabidopsis, maize and barley, respectively. Conclusions CHERIPIC allows users to rapidly analyse bulk segregant sequence data and we have made it available as a pre-packaged binary with all dependencies for Linux and MacOS and as Galaxy tool
Fortalecimiento de saberes tradicionales campesinos en la IED Alfonso Pab贸n Pab贸n
Contribuir a la articulaci贸n de los saberes tradicionales campesinos y educaci贸n rural en la Instituci贸n Educativa Departamental Alfonso Pab贸n Pab贸n, Fosca, Colombia a partir de su caracterizaci贸n.Los saberes tradicionales campesinos encuentran en las escuelas rurales un nicho propicio para su preservaci贸n. El presente proyecto plantea la articulaci贸n entre saberes tradicionales campesinos y educaci贸n rural en la IED Alfonso Pab贸n Pab贸n, S谩name, Fosca, Cundinamarca. Bajo un enfoque de Investigaci贸n Etnogr谩fica se recopila informaci贸n cuantitativa y cualitativa que permita el dise帽o conjunto con estudiantes y acudientes de una estrategia de educaci贸n popular que permita el rescate de estos saberes. Los resultados obtenidos han dado cuenta de la importancia que dan los padres de familia a este rescate de saberes tradicionales y la desconexi贸n existente entre la educaci贸n rural y las realidades del contexto. Esta estrategia constituye una importante oportunidad para repensar la pr谩ctica educativa, permitiendo su di谩logo con las formas de producci贸n de conocimiento de las comunidades aleda帽as
Plan de negocios para el montaje de una tienda deportiva especializada, en el municipio de Villavicencio
285 P谩ginas.El negocio evaluado en el presente documento consiste en la creaci贸n de una tienda deportiva especializada, que estar谩 ubicada en la ciudad de Villavicencio. La tienda contar谩 con la m谩s completa variedad de ropa, implementos y art铆culos deportivos de las m谩s reconocidas marcas, requeridos por las disciplinas de mayor auge y prospectiva en la ciudad siendo estas tenis, patinaje, f煤tbol, ciclismo y nataci贸n, priorizadas a partir de los resultados de la investigaci贸n de mercados que sustenta este plan. La principal fuente de ingresos del negocio ser谩 la comercializaci贸n de los productos deportivos en el punto de venta, siendo el dise帽o de este bastante atractivo respondiendo en su estilo al de las tienda por departamento, dedicando un espacio espec铆fico para cada una de las disciplinas deportivas, facilitando la implementaci贸n de estrategias como venta cruzada e incremento de los ingresos de la compa帽铆a a trav茅s de sku por transacci贸n y valorizaci贸n del ticket promedio. El modelo de administraci贸n de clientes, que incluir谩 la identificaci贸n de necesidades, el fortalecimiento de relaciones, la retroalimentaci贸n constante con grupos de inter茅s (clubes deportivos, escuelas de instrucci贸n, grupos de aficionados, etc),la participaci贸n y promoci贸n de eventos deportivos, todo ello soportado en el uso de redes sociales, as铆 como la oferta de productos especializados por pedido v铆a cat谩logo, ser谩n herramientas para la atracci贸n de un mercado de clientes insuficientemente atendido por la competencia local
Prevalence and molecular epidemiology of bovine leukemia virus in Colombian cattle
Bovine leukemia virus (BLV) is one of the five agents considered most significant for cattle. It is important to determine the prevalence and molecular epidemiology of BLV throughout the country in order to gain a more thorough understanding of the current situation of BLV and to reveal the possibility of masked genotypes that the primers used by OIE are unable to identify. Blood samples were collected at random from 289 cows distributed in 75 farms across the country. PCR amplification of env, gag and tax gene segments was performed. The obtained amplicons were sequenced and then subjected to phylogenetic analyses. A total of 62% of the cows present at 92% of the farms were BLV-positive for gag fragment. Genotype 1 was exclusively detected by env gene segment when analyzed using previously reported primers. However, tax gene analysis revealed circulation of genotype 6 variants, which were also detected based on env gene analysis with newly designed primers. These results indicate that current genotyping approaches based on partial env sequencing may bias BLV genetic variability approaches and underestimate the diversity of the detected BLV genotypes. This report is one of the first molecular and epidemiological studies of BLV conducted in Colombia, which contributes to the global epidemiology of the virus; it also highlights the substantial impact of BLV on the country's livestock and thus is a useful resource for farmers and government entities
Transcriptomics reveal potential vaccine antigens and a drastic increase of upregulated genes during Theileria parva development from arthropod to bovine infective stages
<div><p><i>Theileria parva</i> is a protozoan parasite transmitted by the brown ear tick <i>Rhipicephalus appendiculatus</i> that causes East Coast fever (ECF) in cattle, resulting in substantial economic losses in the regions of southern, eastern and central Africa. The schizont form of the parasite transforms the bovine host lymphocytes into actively proliferating cancer-like cells. However, how <i>T</i>. <i>parva</i> causes bovine host cells to proliferate and maintain a cancerous phenotype following infection is still poorly understood. On the other hand, current efforts to develop improved vaccines have identified only a few candidate antigens. In the present paper, we report the first comparative transcriptomic analysis throughout the course of <i>T</i>. <i>parva</i> infection. We observed that the development of sporoblast into sporozoite and then the establishment in the host cells as schizont is accompanied by a drastic increase of upregulated genes in the schizont stage of the parasite. In contrast, the ten highest gene expression values occurred in the arthropod vector stages. A comparative analysis showed that 2845 genes were upregulated in both sporozoite and schizont stages compared to the sporoblast. In addition, 647 were upregulated only in the sporozoite whereas 310 were only upregulated in the schizont. We detected low p67 expression in the schizont stage, an unexpected finding considering that p67 has been reported as a sporozoite stage-specific gene. In contrast, we found that transcription of p67 was 20 times higher in the sporoblast than in the sporozoite. Using the expression profiles of recently identified candidate vaccine antigens as a benchmark for selection for novel potential vaccine candidates, we identified three genes with expression similar to p67 and several other genes similar to Tp1鈥擳p10 schizont vaccine antigens. We propose that the antigenicity or chemotherapeutic potential of this panel of new candidate antigens be further investigated. Structural comparisons of the transcripts generated here with the existing gene models for the respective loci revealed indels. Our findings can be used to improve the structural annotation of the <i>T</i>. <i>parva</i> genome, and the identification of alternatively spliced transcripts.</p></div
Mi escuela virtual de Educaci贸n Matem谩tica
esde el a帽o 2004 la Licenciatura en Matem谩ticas, consecuente con el principio de pertinencia de la Investigaci贸n en la Universidad de Cundinamarca, emprendi贸 acciones inmediatas orientadas a generar procesos de innovaci贸n modernizadora en la formaci贸n de docentes investigadores en Educaci贸n Matem谩tica que contribuyeran con la construcci贸n gradual de bases s贸lidas para la l铆nea de investigaci贸n del programa10, eje articulador del proyecto curricular
Potential for re-emergence of wheat stem rust in the United Kingdom
Wheat stem rust, a devastating disease of wheat and barley caused by the fungal pathogen Puccinia graminis f. sp. tritici, was largely eradicated in Western Europe during the mid-to-late twentieth century. However, isolated outbreaks have occurred in recent years. Here we investigate whether a lack of resistance in modern European varieties, increased presence of its alternate host barberry and changes in climatic conditions could be facilitating its resurgence. We report the first wheat stem rust occurrence in the United Kingdom in nearly 60 years, with only 20% of UK wheat varieties resistant to this strain. Climate changes over the past 25 years also suggest increasingly conducive conditions for infection. Furthermore, we document the first occurrence in decades of P. graminis on barberry in the UK. Our data illustrate that wheat stem rust does occur in the UK and, when climatic conditions are conducive, could severely harm wheat and barley production.</p
Fortalecimiento de saberes tradicionales campesinos en la IED Alfonso Pab贸n Pab贸n
Contribuir a la articulaci贸n de los saberes tradicionales campesinos y educaci贸n rural en la Instituci贸n Educativa Departamental Alfonso Pab贸n Pab贸n, Fosca, Colombia a partir de su caracterizaci贸n.Los saberes tradicionales campesinos encuentran en las escuelas rurales un nicho propicio para su preservaci贸n. El presente proyecto plantea la articulaci贸n entre saberes tradicionales campesinos y educaci贸n rural en la IED Alfonso Pab贸n Pab贸n, S谩name, Fosca, Cundinamarca. Bajo un enfoque de Investigaci贸n Etnogr谩fica se recopila informaci贸n cuantitativa y cualitativa que permita el dise帽o conjunto con estudiantes y acudientes de una estrategia de educaci贸n popular que permita el rescate de estos saberes. Los resultados obtenidos han dado cuenta de la importancia que dan los padres de familia a este rescate de saberes tradicionales y la desconexi贸n existente entre la educaci贸n rural y las realidades del contexto. Esta estrategia constituye una importante oportunidad para repensar la pr谩ctica educativa, permitiendo su di谩logo con las formas de producci贸n de conocimiento de las comunidades aleda帽as
FORTALECIMIENTO DE SABERES TRADICIONALES CAMPESINOS EN LA IED ALFONSO PAB脫N PAB脫N
Los saberes tradicionales campesinos encuentran en las escuelas rurales un nicho propicio para su preservaci贸n. El presente proyecto plantea la articulaci贸n entre saberes tradicionales campesinos y educaci贸n rural en la IED Alfonso Pab贸n Pab贸n, S谩name, Fosca, Cundinamarca. Bajo un enfoque de Etnograf铆a Educativa se recopil贸 informaci贸n cuantitativa y cualitativa que permitiera el dise帽o conjunto con estudiantes y acudientes de una estrategia de educaci贸n popular que permita el rescate de estos saberes. Los resultados obtenidos han dado cuenta de la importancia que dan los padres de familia a este rescate de saberes tradicionales y la desconexi贸n existente entre la educaci贸n rural y las realidades del contexto. Esta estrategia constituye una importante oportunidad para repensar la pr谩ctica educativa, permitiendo su di谩logo con las formas de producci贸n de conocimiento de las comunidades aleda帽as