85 research outputs found
Structure of fungal communities in sequencing batch reactors operated at different salinities for the selection of triacylglyceride-producers from a fish-canning lipid-rich waste stream
Oleaginous fungi natively accumulate large amounts of triacylglycerides (TAG), widely used as precursors for
sustainable biodiesel production. However, little attention has been paid to the diversity and roles of fungal
mixed microbial cultures (MMCs) in sequencing batch reactors (SBR). In this study, a lipid-rich stream produced
in the fish-canning industry was used as a substrate in two laboratory-scale SBRs operated under the feast/famine
(F/F) regime to enrich microorganisms with high TAG-storage ability, under two different concentrations of NaCl
(SBR-N: 0.5 g/L; SBR-S: 10 g/L). The size of the fungal community in the enriched activated sludge (EAS) was
analyzed using 18S rRNA-based qPCR, and the fungal community structure was determined by Illumina
sequencing. The different selective pressures (feeding strategy and control of pH) implemented in the enrichment
SBRs throughout operation increased the abundance of total fungi. In general, there was an enrichment of genera
previously identified as TAG-accumulating fungi (Apiotrichum, Candida, Cutaneotrichosporon, Geotrichum,
Haglerozyma, Metarhizium, Mortierella, Saccharomycopsis, and Yarrowia) in both SBRs. However, the observed
increase of their relative abundances throughout operation was not significantly linked to a higher TAG
accumulation.Spanish Government CTQ2017 - 83225-C2 - 1- R
CTQ2017 - 83225-C2 - 2-R
PID-2020 - 112550RB-C2
Dynamics of PHA-Accumulating Bacterial Communities Fed with Lipid-Rich Liquid Effluents from Fish-Canning Industries
This research was supported by the Spanish Government (Spanish Ministry of Science and Innovation) through the TREASURE-MICROSALT (CTQ2017-83225-C2-2-R) and ECOPOLYVER-MACROPOLYVER (PID2020-112550RC22) projects.The biosynthesis of polyhydroxyalkanoates (PHAs) from industrial wastes by mixed
microbial cultures (MMCs) enriched in PHA-accumulating bacteria is a promising technology to
replace petroleum-based plastics. However, the populations’ dynamics in the PHA-accumulating
MMCs are not well known. Therefore, the main objective of this study was to address the shifts
in the size and structure of the bacterial communities in two lab-scale sequencing batch reactors
(SBRs) fed with fish-canning effluents and operated under non-saline (SBR-N, 0.5 g NaCl/L) or
saline (SBR-S, 10 g NaCl/L) conditions, by using a combination of quantitative PCR and Illumina
sequencing of bacterial 16S rRNA genes. A double growth limitation (DGL) strategy, in which
nitrogen availability was limited and uncoupled to carbon addition, strongly modulated the relative
abundances of the PHA-accumulating bacteria, leading to an increase in the accumulation of PHAs,
independently of the saline conditions (average 9.04 wt% and 11.69 wt%, maximum yields 22.03 wt%
and 26.33% SBR-N and SBR-S, respectively). On the other hand, no correlations were found among
the PHAs accumulation yields and the absolute abundances of total Bacteria, which decreased through
time in the SBR-N and did not present statistical differences in the SBR-S. Acinetobacter, Calothrix,
Dyella, Flavobacterium, Novosphingobium, Qipengyuania, and Tsukamurella were key PHA-accumulating
genera in both SBRs under the DGL strategy, which was revealed as a successful tool to obtain a
PHA-enriched MMC using fish-canning effluents.Spanish Government (Spanish Ministry of Science and Innovation) CTQ2017-83225-C2-2-R
PID2020-112550RC2
Revealing the dissimilar structure of microbial communities in different WWTPs that treat fish-canning wastewater with different NaCl content
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. This research was supported by the Spanish Government (Spanish Ministry of Science and Innovation) through TREASURE-TECHNOSALT (CTQ2017-83225-C2-1-R) and TREASURE-MICROSALT (CTQ2017-83225-C2-2-R) projects.
The authors Alba Roibas-Rozas and Anuska Mosquera-Corral belong to the Galician Competitive Research Group GRC-ED431C 2017-29 and to the CRETUS Strategic Partnership (ED431E 2018/01) . All these programmes are co-funded by FEDER (UE) . Funding for open access charge: Universidad de Granada/CBUA.Studies that characterize the microbial communities in wastewater treatment plants (WWTPs) are numerous, yet
similar studies in industrial WWTPs treating fish-canning effluents are limited. The microbial communities in
samples of 4 fish-canning WWTPs that operated under different NaCl concentrations were investigated by qPCR
and partial 16S rRNA gene Illumina sequencing. The absolute abundances of key microbial populations (Total
Bacteria, Archaea and Fungi, ammonium oxidizing bacteria (AOB), Mycolata, Candidatus Microthrix, Ca. Accumulibacter
and Ca. Competibacter) presented statistical differences among the WWTPs. The NaCl concentration
negatively affected the absolute abundance of Bacteria and Fungi, filamentous, and phosphate (PAO) and
glycogen (GAO) accumulating bacteria, while AOB and Ca. Microthrix populations were statistically higher in
the WWTP with higher NaCl contents. On the other hand, the main bacterial operational taxonomic units (OTUs)
were classified as members of Kouleothrix (Chloroflexia, Chloroflexi) and Tetrasphaera (Actinomycetia, Actinobacteria),
family Beijerinckiaceae (Alphaproteobacteria, Proteobacteria), order Betaproteobacteriales (Gammaproteobacteria,
Proteobacteria), Sphingobacteriales (Sphingobacteriia, Bacteroidetes) and Frankiales (Actinobacteria,
Actinobacteria), class Anaerolineae (Chloroflexi), phylum Chloroflexi and Bacteria_unclassified. The structure of the
bacterial community was highly dissimilar among the 4 WWTPs, as the identities of the dominant OTUs differed
significantly among them. Therefore, the individual characteristics of the different WWTPs, mainly NaCl concentration,
were responsible for the narrow assemblage of the bacterial communities. Different OTUs belonging
to the phyla Actinobacteria, Bacteroidetes, Chloroflexi, Gemmatimonadetes and Proteobacteria were revealed as salttolerant.
Taking into account these results, NaCl content was an important driver of the abundance of microbial
populations and the bacterial community structure in the analysed industrial facilities.Spanish Government (Spanish Ministry of Science and Innovation) CTQ2017-83225-C2-1-R
CTQ2017-83225-C2-2-REuropean CommissionGalician Competitive Research Group GRC-ED431C 2017-29CRETUS Strategic Partnership ED431E 2018/0
El proyecto Alteregos, reflexiones y acciones en torno a la creación, las artes y la cultura en la universidad
La especialización propia de los estudios universitarios parece ir en aumento en la actualidad. Una posible consecuencia no deseada de esta tendencia podría suponer que los miembros de la comunidad universitaria postergasen las actividades relacionadas con la creación, o el consumo, artístico y cultural que resulten ajenas al propio campo de especialización. En este sentido apuntan ciertos indicios presentes en los trabajos de campo llevados a cabo por el ‘Observatorio Cultural del Proyecto Atalaya’. El Proyecto AlterEgos se plantea como un espacio dónde cuestionarse esa posible postergación. En este artículo se recogen algunas de las reflexiones y de las acciones surgidas durante la gestación y desarrollo de dicho proyecto
Identification of Promiscuous African Swine Fever Virus T-Cell Determinants Using a Multiple Technical Approach
The development of subunit vaccines against African swine fever (ASF) is mainly hindered by the lack of knowledge regarding the specific ASF virus (ASFV) antigens involved in protection. As a good example, the identity of ASFV-specific CD8+ T-cell determinants remains largely unknown, despite their protective role being established a long time ago. Aiming to identify them, we implemented the IFNγ ELISpot as readout assay, using as effector cells peripheral blood mononuclear cells (PBMCs) from pigs surviving experimental challenge with Georgia2007/1. As stimuli for the ELISpot, ASFV-specific peptides or full-length proteins identified by three complementary strategies were used. In silico prediction of specific CD8+ T-cell epitopes allowed identifying a 19-mer peptide from MGF100-1L, as frequently recognized by surviving pigs. Complementarily, the repertoire of SLA I-bound peptides identified in ASFV-infected porcine alveolar macrophages (PAMs), allowed the characterization of five additional SLA I-restricted ASFV-specific epitopes. Finally, in vitro stimulation studies using fibroblasts transfected with plasmids encoding full-length ASFV proteins, led to the identification of MGF505-7R, A238L and MGF100-1L as promiscuously recognized antigens. Interestingly, each one of these proteins contain individual peptides recognized by surviving pigs. Identification of the same ASFV determinants by means of such different approaches reinforce the results presented here.info:eu-repo/semantics/publishedVersio
Seroprevalencia de Trypanosoma cruzi en niños de Veracruz, México: línea de base epidemiológica para un modelo de control fundamentado en la transmisión activa de la enfermedad de Chagas
Introduction. In 2021 the Ministry of Health of Mexico and the Pan American Health Organization launched an initiative to interrupt intra-domiciliary vector transmission of Chagas Disease (CD), based on knowledge of the prevalence of this disease in children. The Mexican State of Veracruz was the leading of this initiative.Objective. To estimate the seroprevalence of Trypanosoma cruzi infection among children under 15 years of age from rural areas of Veracruz, Mexico.Materials and methods. Eight priority localities from the Municipality of Tempoal, Veracruz, were identified for baseline serology. Blood samples were collected on filter paperfrom 817 individuals (June-August 2017) for screening using a third-generation enzyme immunoassay. Reactive cases were confirmed by indirect hemagglutination (HAI), Enzyme linked immunosorbent assay (ELISA), and indirect immunofluorescence (IIF) tests with peripheral blood serum samples. Seroprevalence and its 95% confidence interval (95% C.I.) were calculated.. Results. In the localities Citlaltepetl, Cornizuelo, Cruz de Palma y Rancho Nuevo, CD cases were confirmed in children under 15 years of age with a seroprevalence of 1,9% (95% C.I.: 1,12-3,16%). Conclusions. These results indicate that these communities present recent transmission of CD and allow establishing an epidemiological baseline for the design and implementation of a model based on activities focused on geographic areas with active transmission to advance towards the elimination of intra-domiciliary vector transmission of CD in Mexico.Introducción. En 2021 la Secretaría de Salud de México y la Organización Panamericana de la Salud lanzaron una iniciativa para la interrupción de la transmisión vectorial intradomiciliaria de la enfermedad de Chagas (EC), fundamentada en el conocimiento de la prevalencia de esta enfermedad en población infantil. El estado mexicano de Veracruz fue punta de lanza de esta iniciativa.Objetivo. Estimar la seroprevalencia de infección por Trypanosoma cruzi en menores de 15 años de localidades rurales de Veracruz, México.Materiales y métodos. Se identificaron 8 localidades prioritarias para la serología basal en el municipio de Tempoal, Veracruz. Se colectaron muestra de sangre en papel filtro de 817 individuos (junio-agosto de 2017) para su tamizaje usando un inmunoensayo enzimático de tercera generación. Los casos reactivos del tamizaje se confirmaron mediante pruebas de hemaglutinación indirecta (HAI), ensayo por inmunoabsorción ligado a enzimas (ELISA) e inmunofluorescencia indirecta (IFI) en muestras de suero colectadas en tubo. Se calculó la seroprevalencia y su intervalo de confianza del 95% (I.C. 95%).Resultados. En las localidades Citlaltépetl, Cornizuelo, Cruz de Palma y Rancho Nuevo se confirmaron casos de la EC en menores de 15 años con una seroprevalencia de 1,9% (I.C. 95%: 1,12-3,16%).Conclusiones. Los resultados indican que estas comunidades presentan transmisión reciente de la EC y permiten establecer una línea de base epidemiológica para el diseño e implementación de un modelo fundamentado en acciones dirigidas a áreas geográficas con transmisión activa para avanzar hacia la eliminación de transmisión vectorial intradomiciliar de la EC en México
The Research Journey as a Challenge Towards New Trends
The academic community of the department of Risaralda, in its permanent interest in evidencing the results of the research processes that are carried out from the Higher Education Institutions and as a product of the VI meeting of researchers of the department of Risaralda held in November 2021 presents its work: “The journey of research as a challenge towards new trends”, which reflects the result of the latest research and advances in different lines of knowledge in Agricultural Sciences, Health Sciences, Social Sciences and Technology and Information Sciences, which seek to solve and meet the demands of the different sectors.
This work would not have been possible without the help of each of the teachers, researchers and authors who presented their articles that make up each of the chapters of the book, to them our gratitude for their commitment, dedication and commitment, since their sole purpose is to contribute from the academy and science to scientific and technological development in the search for the solution of problems and thus contribute to transform the reality of our society and communities. We also wish to extend our
gratitude to the institutions of the Network that made this publication possible: UTP, UCP, UNAD, UNIREMINGTON; UNISARC, CIAF, Universidad Libre, Uniclaretiana, Fundación Universitaria Comfamiliar and UNIMINUTO, institutions that in one way or another allowed this work to become a reality, which we hope will be of interest to you.Preface............................................................................................................................7
Chapter 1. Technologies and Engineering
Towards a humanization in Engineering using soft skills in training
in Engineers.............................................................................................................11
Omar Iván Trejos Buriticá1, Luis Eduardo Muñoz Guerrero
Innovative materials in construction: review from a bibliometric
analysis....................................................................................................................27
Cristian Osorio Gómez, Daniel Aristizábal Torres, Alejandro Alzate Buitrago,
Cristhian Camilo Amariles López
Bibliometric review of disaster risk management: progress, trends,
and challenges.........................................................................................................51
Alejandro Alzate Buitrago, Gloria Milena Molina Vinasco.
Incidence of land coverage and geology, in the unstability of lands
of the micro-basin of the Combia creek, Pereira, Risaralda....................................73
Alejandro Alzate Buitrago, Daniel Aristizábal Torres.
Chapter 2. Arts, Humanities, and Social Sciences
Training experience with teachers teaching mathematics using the
inquiry methodology ...............................................................................................95
Vivian Libeth Uzuriaga López, Héctor Gerardo Sánchez Bedoya.
Interpretation of the multiple representations of the fears associated
to the boarding of limited visual patients in the elective I students’ written
productions and low vision ...................................................................................113
Eliana Bermúdez Cardona, Ana María Agudelo Guevara, Caterine Villamarín Acosta.
The relevance of local knowledge in social sciences............................................131
Alberto Antonio Berón Ospina, Isabel Cristina Castillo Quintero.
Basic education students’ conceptions of conflict a view from the peace
for the education....................................................................................................143
Astrid Milena Calderón Cárdenas,Carolina Aguirre Arias, Carolina Franco Ossa,
Martha Cecilia Gutiérrez Giraldo, Orfa Buitrago.
Comprehensive risk prevention in educational settings: an interdisciplinary
and socio-educational approach ............................................................................163
Olga María Henao Trujillo, Claudia María López Ortiz.
Chapter 3. Natural and Agricultural Sciences
Physicochemical characterization of three substrates used in the deep
bedding system in swine .......................................................................................175
Juan Manuel Sánchez Rubio, Andrés Felipe Arias Roldan, Jesús Arturo Rincón Sanz,
Jaime Andrés Betancourt Vásquez.
Periodic solutions in AFM models........................................................................187
Daniel Cortés Zapata, Alexander Gutiérrez Gutiérrez.
Phenology in flower and fruit of Rubus glaucus benth. Cv. Thornless
in Risaralda: elements for phytosanitary management .........................................199
Shirley Palacios Castro, Andrés Alfonso Patiño Martínez, James Montoya Lerma,
Ricardo Flórez, Harry Josué Pérez.
Socio-economic and technical characterization of the cultivation of
avocado (Persea americana) in Risaralda..............................................................217
Andrés Alfonso Patiño Martínez, Kelly Saudith Castañez Poveda, Eliana Gómez Correa.
Biosecurity management in backyard systems in Santa Rosa de Cabal,
Risaralda................................................................................................................227
Julia Victoria Arredondo Botero, Jaiver Estiben Ocampo Jaramillo, Juan Sebastián Mera Vallejo,
Álvaro de Jesús Aranzazu Hernández.
CONTENTS
Physical-chemical diagnosis of soils in hillside areas with predominance
of Lulo CV. La Selva production system in the department of Risaralda.............241
Adriana Patricia Restrepo Gallón, María Paula Landinez Montes, Jimena Tobón López.
Digestibility of three concentrates used in canine feeding....................................271
María Fernanda Mejía Silva, Valentina Noreña Sánchez, Gastón Adolfo Castaño Jiménez.
Chapter 4. Economic, Administrative, and Accounting Sciences
Financial inclusion in households from socioeconomic strata 1 and 2 in
the city of Pereira ..................................................................................................285
Lindy Neth Perea Mosquera, Marlen Isabel Redondo Ramírez, Angélica Viviana Morales.
Internal marketing strategies as a competitive advantage for the company
Mobilautos SAS de Dosquebradas........................................................................303
Inés Montoya Sánchez, Sandra Patricia Viana Bolaños, Ana María Barrera Rodríguez.
Uses of tourist marketing in the tourist sector of the municipality of Belén
de Umbría, Risaralda.............................................................................................319
Ana María Barrera Rodríguez, Paola Andrea Echeverri Gutiérrez, María Camila Parra Buitrago,
Paola Andrea Martín Muñoz, Angy Paola Ángel Vélez, Luisa Natalia Trejos Ospina.
Territorial prospective of Risaralda department (Colombia), based on
the SDGS...............................................................................................................333
Juan Guillermo Gil García, Samanta Londoño Velásquez.
Chapter 5. Health and Sports Sciences
Performance evaluation in times of pandemic. What do medical
students think?.......................................................................................................353
Samuel Eduardo Trujillo Henao, Rodolfo A. Cabrales Vega, Germán Alberto Moreno Gómez.
The relevance of the therapist’s self and self-reference in the training
of psychologists.....................................................................................................371
Maria Paula Marmolejo Lozano, Mireya Ospina Botero.
Habits related to oral health which influence lifestyle of elder people
in a wellness center for the elderly in Pereira 2020. .............................................387
Isadora Blanco Pérez, Olga Patricia Ramírez Rodríguez, Ángela María Rincón Hurtado.
Analysis of the suicide trend in the Coffee Region in Colombia during
the years 2012-2018 ..............................................................................................405
Germán Alberto Moreno Gómez, Jennifer Nessim Salazar, Jairo Franco Londoño,
Juan Carlos Medina Osorio.
Hind limb long bone fractures in canines and felines...........................................419
María Camila Cruz Vélez, Valentina Herrera Morales, Alba Nydia Restrepo Jiménez, Lina
Marcela Palomino, Gabriel Rodolfo Izquierdo Bravo.
Prevalence of overweight and obesity in children in the rural and urban
area of Risaralda....................................................................................................439
Angela María Álvarez López, Angela Liceth Pérez Rendón, Alejandro Gómez Rodas,
Luis Enrique Isaza Velásquez.
Chapter 6. Architecture, Design and Advertising
The artisan crafts of Risaralda, characteristics, importance, and risks
within the Colombian Coffee Cultural Landscape, CCCL....................................457
Yaffa Nahir Ivette Gómez Barrera, Javier Alfonso López Morales
Desarrollo Económico de la Comarca de Cartagena 2016
El presente informe tiene la finalidad de servir como
una herramienta de diagnóstico de la situación
económica del tejido empresarial de la Comarca de
Cartagena, promoviendo medidas que contribuyen
al desarrollo estratégico de la Comarca.
A partir del estudio y análisis del contexto socioeconómico
sectorial que han realizado los
investigadores especialistas en cada uno de los
sectores de actividad, así como del análisis por parte
de los paneles de expertos consultados, se incluye
a continuación un estudio por sectores de actividad
de las actuaciones que deberían considerarse para
establecer las líneas estratégicas de contenido
económico en la Comarca de Cartagena en los
próximos años. Las políticas que permitan desarrollar
dichas actuaciones asegurarán la competitividad de
las pequeñas y medianas empresas en general y el
adecuado progreso económico de la Comarca en
particular.
Los principales pilares de competitividad sobre los
que deberían asentarse las actuaciones estratégicas
para la comarca se han clasificado por sectores de
actividad atendiendo a las infraestructuras, el
conocimiento (formación y cualificación), el desarrollo
de las tecnologías de la información y las
comunicaciones, la innovación, la financiación y la
eficiencia de las Administraciones Públicas.
El informe se estructura en una primera aproximación
a la situación macroeconómica de la Comarca en
su contexto Regional y Nacional desde el punto de
vista de la oferta y la demanda, así como un detalle
prospectivo de la economía para los próximos meses.
A continuación se detalla el análisis sectorial dividido
en los sectores: turismo, comercio, industria,
agroalimentación y tecnológico. Adicionalmente, se
incluye un análisis y diagnóstico económicofinanciero
a nivel microeconómico de las pymes de
la Comarca. Por último, se incluyen a modo de
resumen las principales conclusiones y
recomendaciones derivadas del estudi
Lo glocal y el turismo. Nuevos paradigmas de interpretación.
El estudio del turismo se realiza desde múltiples escalas y enfoques, este libro aborda muchos temas que es necesario discutir desde diversas perspectivas; es el caso de la reflexión sobre la propia disciplina y sus conceptos, así como los asuntos específicos referidos al impacto territorial, los tipos de turismo, las cuestiones ambientales, el tema de la pobreza, la competitividad, las políticas públicas, el papel de las universidades, las áreas naturales protegidas, la sustentabilidad, la cultura, el desarrollo, la seguridad, todos temas centrales documentados y expuestos con originalidad y dominio del asunto. Lo multiescalar es básico para la comprensión del sistema turístico, sistema formado de procesos globales, regionales y locales. El eje de discusión del libro es lo glocal, esa interacción entre lo nacional y local con lo global
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