Total : projeto de cobertura de entorno em grandes eventos esportivos

Monografia (graduação)—Universidade de Brasília, Faculdade de Comunicação, Departamento de Jornalismo, 2014.Redação de projeto editorial da revista TOTAL, especializada em cobertura do entorno dos maiores eventos esportivos. O produto apresenta indicações para apuração de temas sobre a influência desses acontecimentos nos setores da sociedade alheios ao esporte, a partir de estudos sobre a história do esporte, da mobilização do público e da abordagem da imprensa. Além disso, o trabalho prevê linhas de investigação jornalística a partir da pesquisa sobre as sedes de cada grande competição até os Jogos Olímpicos do Rio de Janeiro, em 2016

Sustainable synthesis of uridine-5′-monophosphate analogues by immobilized uracil phosphoribosyltransferase from Thermus thermophilus

Nowadays enzymatic synthesis of nucleic acid derivatives is gaining momentum over traditional chemical synthetic processes. Biotransformations catalyzed by whole cells or enzymes offer an ecofriendly and efficient alternative to the traditional multistep chemical methods, avoiding the use of chemical reagents and organic solvents that are expensive and environmentally harmful. Herein we report for the first time the covalent immobilization a uracil phosphoribosyltransferase (UPRT). In this sense, UPRT from Thermus thermophilus HB8 was immobilized onto glutaraldehyde-activated MagReSyn®Amine magnetic iron oxide porous microparticles (MTtUPRT). According to the catalyst load experiments, MTtUPRT3 was selected as optimal biocatalyst for further studies. MTtUPRT3 was active and stable in a broad range of temperature (70–100 °C) and in the pH interval 6–8, displaying maximum activity at 100 °C and pH 7 (activity 968 IU/gsupport, retained activity 100%). In addition, MTtUPRT3 could be reused up to 8 times in the synthesis of uridine-5′-monophosphate (UMP). Finally, MTtUPRT3 was successfully applied in the sustainable synthesis of different 5-modified uridine-5′-monophosphates at short times. Taking into account these results, MTtUPRT3 would emerge as a valuable biocatalyst for the synthesis of nucleoside monophosphates through an efficient and environmentally friendly methodology

Attempted predation by introduced salmonids on a native catfish in an Andean River in Argentina

Interspecific predator-prey interactions play an important role in the structure of communities. However, predation events in the wild are rare, and difficult to observe, and rarely documented. The objective of this work is to report the attempted predation on Hatcheria macraei by some of the salmonids with which it occurs in syntropy. During a survey of ichthyofauna in the Castaño River, an adult specimen of H. macraei was captured with evidence of a bite on the caudal fin. Despite not having been able to make the specific identification of the predator in the predation attempt on H. macraei, this report adds to the enormous base of information on the devastating effects of introduced salmonids on aquatic ecosystems and the native species that live there inhabit. It is necessary to control the expansion of salmonids, by protecting areas of high value for native fish and prohibiting future aquaculture projects to prevent the invasion of this species to new locations in these aquatic ecosystems

¿EXISTE RELACIÓN ENTRE EL PARASITISMO Y LA AUTOTOMÍA DE COLA EN LAGARTIJAS? CASO DE ESTUDIO EN LIOLAEMUS DARWINII (IGUANIA: LIOLAEMIDAE)

Tail autotomy acts as anti-predatory mechanism in a great diversity of lizard species. Research links tail loss with various aspects of lizard life history. However, parasitic aspects are not taken into consideration, despite being a variable that greatly influences reptile ecology. For the first time, it is proposed that the existence of a relationship between the caudal autotomy of a lizard species is related to its parasitic condition. We studied 30 adult specimens of Liolaemus darwinii Bell, 1843 in the desert of Monte, San Juan province, Argentina. Autotomy was used as a direct measure of predator-prey interaction. A series of variables were analyzed that could be related to tail autotomy. The following variables were studied using generalized linear models: sex, environments (disturbed vs. conserved), condition (parasitized vs. not parasitized), parasitic intensity, weights (g) and snout-vent length (Lhc). The dependent variable (presence of tail: 1, absence of tail: 0) was adjusted to a binomial distribution, whose link function is logit (logistic regression). Only the variable parasitized vs. non-parasitized condition was significantly associated with tail autotomy. Parasitized individuals all have cut tails or in some stage of regeneration. Individuals that were not parasitized had complete tails (intact). The results establish for the first time a relationship between parasitism and tail loss in L. darwinii. The results are discussed in a behavioral ecological context and physiological resource allocation. In addition, our work is an approach to understanding the role of parasites in the ecology of L. darwinii in the Monte desert in Argentina.La autotomía de cola es usada como estrategia antipredatoria en diversas especies de lagartijas. Las investigaciones relacionan la pérdida de cola con diversos aspectos en la historia de vida en reptiles. Sin embargo, los aspectos parasitarios no son tenidos en cuenta, a pesar de ser una variable que influye enormemente en la ecología de reptiles. Por primera vez, se propone la existencia de una relación entre la autotomía caudal de una especie de lagartija con su condición parasitaria. Se analizaron 30 ejemplares adultos de Liolaemus darwinii Bell, 1843 capturados en el desierto del Monte, provincia de San Juan, Argentina. Se utilizó la autotomía caudal como medida directa de interacción entre depredador y presa. Se analizaron cinco variables que podrían relacionarse con la autotomía de cola. Para ello, fueron estudiadas mediante modelos lineales generalizados las variables; sexos, ambientes (perturbado/ conservado), condición (parasitado/ no parasitado), intensidad parasitaria, pesos (g) y longitud hocico- cloaca (Lhc). La variable dependiente (presencia cola: 1, ausencia de cola: 0) se ajustó a una dis­tribución binomial, cuya función de enlace es logit (regresión logística). Solo la variable condición parasitado/ no parasitado se asoció significativamente con la autotomía de cola. Los individuos que se encontraban parasitados, presentaban en su mayoría colas cortadas o en alguna etapa de su regeneración. Sin embargo, los individuos que no estaban parasitados presentaban colas completas (intactas). Los resultados establecen por primera vez una relación entre el parasitismo y la pérdida de cola en L. darwinii. Se discuten los resultados en un contexto ecológico comportamental y asignación fisiológica de recurso. Además, nuestro trabajo es una aproximación al entendimiento del rol de los parásitos en la ecología de L. darwinii en el desierto del Monte en Argentina

Engaging adolescents with Down syndrome in an educational video game

Producción CientíficaThis article describes the design, implementation and evaluation of an educational video game that helps individuals with Down syndrome to improve their speech skills, specifically those related to prosody. Special attention has been paid to the design of the user interface, taking into account the cognitive, learning, and attentional limitations of people with Down syndrome. The learning content is conveyed by activities of production and perception of prosodic phenomena, aimed at increasing their communicative competence. These activities are introduced within the narrative of a video game so that the players do not conceive the tool as a mere succession of learning activities, but so that they learn and improve their speech while playing. The evaluation strategy that has been followed involves real users and combines different evaluation activities. Results show a high level of acceptance by participants and also by professionals, speech therapists, and special education teachers.2018-09-01MEC-FEDER Grant TIN2014-59852-R y la Junta de Castilla y León Regional Grant VA145U1

Reproduction and Sexual dimorphism of the endemic andean Gecko Homonota andicola (Squamata: Phyllodactylidae) from Central Western Argentina

Studies of reproductive biology are essential to understanding the life history of species and contribute to determining their conservation status and future protection. The Andean Gecko (Homonota andicola) is a small lizard endemic to central western Argentina and occurs in Monte, Puna, and High Andes environments. Our objective was to determine the reproductive cycle of a population of H. andicola in the Calingasta Department of San Juan, Argentina, to establish its reproductive potential, and determine if the sexes are sexually dimorphic, and, if so, its relationship with reproduction. Homonota andicola started its reproductive activity in early spring. A single egg was produced, and we found that at least two clutches were produced per reproductive cycle. The gonadal cycle varied with the seasons. For males, testicular development exhibited no variation, demonstrating a continuous reproductive state throughout the activity season. We found that sexes were sexually dimorphic in head length and head width, with these variables being more pronounced in males. We attribute this dimorphism to sexual selection favoring males with larger heads. This study is the first contribution to the biology of the species and its conclusions should serve as a starting point for future research in both an ecological context and in terms of biodiversity conservation.Fil: Fernandez Reinoso, Ruben Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Biología; Argentina. Gabinete de Diversidad y Biología de Vertebrados del Árido; ArgentinaFil: Blanco, Graciela Mirta. Universidad Nacional de San Juan. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Biología; ArgentinaFil: Acosta, Juan Carlos. Universidad Nacional de San Juan. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Biología; ArgentinaFil: Corrales Zuñiga, Lucas Anibal. Universidad Nacional de San Juan. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Valdez Ovallez, Franco Miguel. Universidad Nacional de San Juan. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Acosta, Rodrigo. Universidad Nacional de San Juan. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Biología; Argentin

Homonota horrida (South American Marked Gecko): Aquatic locomotion

On 26 November 2017 at 2134 h, in at La Majadita, Valle Fértil, San Juan, Argentina (30.7152°S, 67.4940°W; WGS 84; 1006 m elev.), an adult H. horrida was observed floating on water in a slow-flowing stream (Fig. 1),while hunting hemipterans (Gerridae). The lizard was suspended on the surface of the water and swam in it, making snake-like motions, to try to capture approaching insects.Fil: Valdez Ovallez, Franco Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ciencias Exactas, Físicas y Naturales; ArgentinaFil: Gómez Alés, Carlos Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ciencias Exactas, Físicas y Naturales; ArgentinaFil: Acosta, Juan Carlos. Universidad Nacional de San Juan. Facultad de Ciencias Exactas, Físicas y Naturales; ArgentinaFil: Corrales, Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ciencias Exactas, Físicas y Naturales; ArgentinaFil: Fernández, Rubén. Universidad Nacional de San Juan. Facultad de Ciencias Exactas, Físicas y Naturales; ArgentinaFil: Acosta, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Biología; ArgentinaFil: Martínez, Tomás Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Ciencias Exactas, Físicas y Naturales; ArgentinaFil: Blanco, Mirta Blanco. Universidad Nacional de San Juan. Facultad de Ciencias Exactas, Físicas y Naturales; Argentin

A recurrent nonsense mutation occurring as a de novo event in a patient with recessive dystrophic epidermolysis bullosa

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Recombinant pharmaceuticals from microbial cells : a 2015 update

Altres ajuts: MARATÓ TV3: 2013-3930, 2013-132031Diabetes, growth or clotting disorders are among the spectrum of human diseases related to protein absence or malfunction. Since these pathologies cannot be yet regularly treated by gene therapy, the administration of functional proteins produced ex vivo is required. As both protein extraction from natural producers and chemical synthesis undergo inherent constraints that limit regular large-scale production, recombinant DNA technologies have rapidly become a choice for therapeutic protein production. The spectrum of organisms exploited as recombinant cell factories has expanded from the early predominating Escherichia coli to alternative bacteria, yeasts, insect cells and especially mammalian cells, which benefit from metabolic and protein processing pathways similar to those in human cells. Up to date, around 650 protein drugs have been worldwide approved, among which about 400 are obtained by recombinant technologies. Other 1300 recombinant pharmaceuticals are under development, with a clear tendency towards engineered versions with improved performance and new functionalities regarding the conventional, plain protein species. This trend is exemplified by the examination of the contemporary protein-based drugs developed for cancer treatment

The magic stone : a video game to improve communication skills of people with intellectual disabilities

'The Magic Stone' is a video game whose main aim is to help people with Down syndrome to improve communication skills that have been affected due to their disability, especially those related with prosody. The interface of the video game includes a number of elements to motivate the users to practice and train their pronunciation. The usability tests of the system have reported high degrees of satisfaction of users and trainers. Perception tests have permitted to confirm that players improve the use of prosody with the us