Cold Induction of EARLI1, a Putative Arabidopsis Lipid Transfer Protein, Is Light and Calcium Dependent

As sessile organisms, plants must adapt to their environment. One approach toward understanding this adaptation is to investigate environmental regulation of gene expression. Our focus is on the environmental regulation of EARLI1, which is activated by cold and long-day photoperiods. Cold activation of EARLI1 in short-day photoperiods is slow, requiring several hours at 4ºC to detect an increase in mRNA abundance. EARLI1 is not efficiently cold-activated in etiolated seedlings, suggesting that photomorphogenesis is necessary for its cold activation. Cold activation of EARLI1 is inhibited in the presence of the calcium channel blocker lanthanum chloride or the calcium chelator EGTA. Addition of the calcium ionophore Bay K8644 results in cold-independent activation of EARLI1. These data suggest that EARLI1 is not an immediate target of the cold response, and that calcium flux affects its expression. EARLI1 is a putative secreted protein and has motifs found in lipid transfer proteins. Over-expression of EARLI1 in transgenic plants results in reduced electrolyte leakage during freezing damage, suggesting that EARLI1 may affect membrane or cell wall stability in response to low temperature stress

A Service-Based Framework for Flexible Business Processes

This article describes a framework for the design and enactment of flexible and adaptive business processes. It combines design-time and run-time mechanisms to offer a single integrated solution. The design-time environment supports the specification of process-drivenWeb applications with Quality of Service (QoS) constraints and monitoring annotations. The runtime identifies the actual services, from the QoS perspective, oversees the execution through monitoring, and reacts to failures and infringement of QoS constraints. The article also discusses these issues on a proof of concept application developed for an industrial supply chain scenario

Development of a CO2 sensor for extracorporeal life support applications

Measurement of carbon dioxide (CO2) in medical applications is a well-established method for monitoring patient’s pulmonary function in a noninvasive way widely used in emergency, intensive care, and during anesthesia. Even in extracorporeal-life support applications, such as Extracorporeal Carbon Dioxide Removal (ECCO2R), Extracorporeal Membrane Oxygenation (ECMO), and cardiopulmonary by-pass (CPB), measurement of the CO2 concentration in the membrane oxygenator exhaust gas is proven to be useful to evaluate the treatment progress as well as the performance of the membrane oxygenator. In this paper, we present a new optical sensor specifically designed for the measurement of CO2 concentration in oxygenator exhaust gas. Further, the developed sensor allows measurement of the gas flow applied to the membrane oxygenator as well as the estimation of the CO2 removal rate. A heating module is implemented within the sensor to avoid water vapor condensation. Effects of temperature on the sensor optical elements of the sensors are disclosed, as well as a method to avoid signal–temperature dependency. The newly developed sensor has been tested and compared against a reference device routinely used in clinical practice in both laboratory and in vivo conditions. Results show that sensor accuracy fulfills the requirements of the ISO standard, and that is suitable for clinical applications

Kidney transplantation with presymptomatic COVID-19–positive surgeon

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A synopsis based approach for XML fast approximate querying

In the last few years, XML has spread in many application fields and today it is used as a format to exchange data on the web, to ensure inter-operability among applications. Due to this success, the W3C has proposed a new query language, XQuery [25], specifically designed to query XML data. XQuery is a well-defined but rather complex language [14]. In this work we propose a new approach to overcome the problem of the high computational costs required by aggregate queries over massive XML data collections. In traditional relational warehouses [11] a similar problem is solved by means of fast approximate queries, that use concise data statistics based on histograms or on other statistical techniques. Their most common application is for aggregate queries in modern decision support systems, where large volumes of data need to be queried, and quick and interactive responses from the DBMS are claimed, e.g., to analyze the data in the warehouse in order to get trend information to evaluate marketing strategies. In such applications, users are often more interested to obtain an approximate answer computed in a short time rather than an exact one obtained in some minutes or, at the worst, hours

A TILLING by sequencing approach to identify induced mutations in sunflower genes

The Targeting Induced Local Lesions in Genomes (TILLING) technology is a reverse genetic strategy broadly applicable to every kind of genome and represents an attractive tool for functional genomic and agronomic applications. It consists of chemical random mutagenesis followed by high-throughput screening of point mutations in targeted genomic regions. Although multiple methods for mutation discovery in amplicons have been described, next-generation sequencing (NGS) is the tool of choice for mutation detection because it quickly allows for the analysis of a large number of amplicons. The aim of the present work was to screen a previously generated sunflower TILLING population and identify alterations in genes involved in several important and complex physiological processes. Twenty-one candidate sunflower genes were chosen as targets for the screening. The TILLING by sequencing strategy allowed us to identify multiple mutations in selected genes and we subsequently validated 16 mutations in 11 different genes through Sanger sequencing. In addition to addressing challenges posed by outcrossing, our detection and validation of mutations in multiple regulatory loci highlights the importance of this sunflower population as a genetic resource

Naturallyoccurring differences in cenh3 affect chromosome segregation in zygotic mitosis of hybrids

The point of attachment of spindle microtubules to metaphase chromosomes is known as the centromere. Plant and animal centromeres are epigenetically specified by a centromere-specific variant of Histone H3, CENH3 (a.k.a. CENP-A). Unlike canonical histones that are invariant, CENH3 proteins are accumulating substitutions at an accelerated rate. This diversification of CENH3 is a conundrum since its role as the key determinant of centromere identity remains a constant across species. Here, we ask whether naturally occurring divergence in CENH3 has functional consequences. We performed functional complementation assays on cenh3-1, a null mutation in Arabidopsis thaliana, using untagged CENH3s from increasingly distant relatives. Contrary to previous results using GFP-tagged CENH3, we find that the essential functions of CENH3 are conserved across a broad evolutionary landscape. CENH3 from a species as distant as the monocot Zea mays can functionally replace A. thaliana CENH3. Plants expressing variant CENH3s that are fertile when selfed show dramatic segregation errors when crossed to a wild-type individual. The progeny of this cross include hybrid diploids, aneuploids with novel genetic rearrangements and haploids that inherit only the genome of the wild-type parent. Importantly, it is always chromosomes from the plant expressing the divergent CENH3 that missegregate. Using chimeras, we show that it is divergence in the fast-evolving N-terminal tail of CENH3 that is causing segregation errors and genome elimination. Furthermore, we analyzed N-terminal tail sequences from plant CENH3s and discovered a modular pattern of sequence conservation. From this we hypothesize that while the essential functions of CENH3 are largely conserved, the N-terminal tail is evolving to adapt to lineage-specific centromeric constraints. Our results demonstrate that this lineage-specific evolution of CENH3 causes inviability and sterility of progeny in crosses, at the same time producing karyotypic variation. Thus, CENH3 evolution can contribute to postzygotic reproductive barriers

Role of Estimated Glomerular Filtration Rate in Clinical Research: The Never-Ending Matter

Background: Chronic kidney disease (CKD) burden is crucial both on a global scale and at individual patient level, affecting morbidity and mortality directly and through its effect on both cardiovascular damage and CKD progression to end-stage-kidney-disease (ESKD). Unfortunately, the awareness of CKD is poor, with few CKD patients conscious of the severity of their health status. The principal biomarker of kidney function is estimated glomerular filtration rate (eGFR). Methods: We searched the literature and present a review article with the aim of summarizing the role of eGFR in clinical research. In particular, we report the eGFR role as a prognostic, enrichment and endpoint biomarker and its role in the early detection of CKD. Results: eGFR has a major role as a biomarker in clinical research. As a prognostic marker, eGFR reduction is associated with cardiovascular events, ESKD and mortality. As an enrichment biomarker, eGFR values are pivotal for selecting patients to be included in randomized and observational studies; it helps to test a pre-defined drug in early CKD or in more advanced CKD allowing also to avoid screening failures and to shorten the duration of clinical trials. Moreover, eGFR decline (expressed as a percentage of reduction from baseline or continuous slope) can be considered a good endpoint in clinic trials overcoming delays whilst waiting for hard endpoints to develop. Conclusions: eGFR is a strong clinical measure for both observational and intervention studies. It is also helpful in screening the general population for kidney disease and, in particular, to increase awareness of CKD

Indoor mobile mapping system and crowd simulation to support school reopening because of covid-19: a case study

Occupancy analyses represent a crucial topic for building performance. At present, this is even true because of the pandemic emergency due to SARS-CoV-2 and the need to support the functional analysis of building spaces in relation to social distancing rules. Moreover, the need to assess the suitability of spaces in high occupancy buildings as the educational ones, for which occupancy evaluations result pivotal to ensure the safety of the end-users in their daily activities, is a priority. The proposed paper investigates the steps that are needed to secure a safe re-opening of an educational building. A case study has been selected as a test site to analyse the re-opening steps as required by Italian protocols and regulations. This analysis supported the school director of a 2-to-10 year old school and its team in the decision-making process that led to the safe school re-opening. Available plants and elevations of the building were collected and a fast digital survey was carried out using the mobile laser scanner technology (iMMS - Indoor Mobile Mapping System) in order to acquire three-dimensional geometries and digital photographic documentation of the spaces. A crowd simulation software (i.e. Oasys MassMotion) was implemented to analyse end-users flows; the social distance parameter was set in its proximity modelling tools in order to check the compliance of spaces and circulation paths to the social distancing protocols. Contextually to the analysis of users flows, a plan of hourly air changes to maintain a high quality of the environments has been defined