A review of the Late Jurassic-Early Cretaceous charophytes from the northern Aquitaine Basin in south-west France

Late Jurassic and Early Cretaceous charophyte assemblages from the northern part of the Aquitaine Basin in south-west France are reviewed here to understand their palaeoecological, palaeobiogeographical and biostratigraphic features. Three sites were studied: the Tithonian-lower Berriasian of Chassiron, and the Berriasian of Cherves-de-Cognac and Angeac-Charente. Abundant porocharaceans, less abundant clavatoraceans and scarce characeans recorded in Cherves-de-Cognac and Angeac-Charente indicate that brackish water environments were substituted by freshwater environments eastwards. The occurrence of Clavator grovesii var. grovesii and morphotypes intermediate with C. grovesii var. discordis in the same areas is significant from a biostratigraphic viewpoint, since these species belong to the Maillardii, Incrassatus and Nurrensis European charophyte biozones, representing the Berriasian. This observation refutes a previous dating of the Angeac-Charente site and highlights the absence of Hauterivian-Barremian records in northern Aquitaine, which is in contrast to the more complete Lower Cretaceous record in southern Aquitaine. These contrasting records could be due to differences in the available sedimentary space produced by the opening of the Bay of Biscay during the Barremian

Stratigraphy of the Haut Var Paleogene continental series (Northeastern Provence, France): New insight on the age of the 'Sables bleutés du Haut Var' Formation

The age of the Paleogene deposits of the Haut Var (Provence, France) has been the subject of debate. Particularly, the ''Calcaire à Bithynies'' and the ''Sables bleutés'' units were ascribed either to the early Eocene or to the Oligocene. A stratigraphical clarification is required in order to precise the paleogeographical relationships of the Haut Var Paleogene sedimentary series with coeval deposits in the neighbouring southern Provence and Subalpine regions and other European domains. The study area is characterized by tectonically separated synclines and grabens filled in by continental Paleogene deposits. Detailed mapping and lithostratigraphical logging, sedimentological and microfacies analysis have been undertaken in order to provide a reliable stratigraphical framework. Biostratigraphical subdivisions were established based on five different fossil groups: mammals, charophytes, gastropods, ostracodes, and foraminifers. Accordingly, five formations are distinguished and dated: ''Calcaire a` Microcodium'' and ''Brèche à Microcodium'' (Danian); ''Marnes à oeufs d'oiseaux'' (Selandian(?)-earliest Ypresian); 'Sables bleutés du Haut Var' (early-late(?) Ypresian); and ''Bourdas conglomerates'' (Rupelian). Particular emphasis is given to the study of the controversial 'Sables bleute´ s du Haut Var' Formation. As a result, correlations have been established between the different syncline and graben areas where Paleocene-Eocene and Oligocene deposits occur. Terrestrial deposits (carbonate paleosols and piedmont alluvial fans) took place during Paleocene times, while fluvial (cross-bedded sands) and lacustrine carbonate deposits developed in a foreland compressional intracontinental basin surrounded by emerged areas and tectonic highs during the early Ypresian. Paleoenvironmental and paleogeo- graphical analysis strengthen the view that a relative isolation characterized the Haut Var area during the early Eocene, probably enhancing episodes of brackish water or evaporitic sedimentation and gastropod endemism. During the late Eocene Pyrenean-Provence tectonic phase, the E-W trending Haut Var overthrusts have been emplaced posteriorly to the deposition of the 'Sables bleute´ s du Haut Var' Fm. Finally, coarse alluvial fan and local lacustrine carbonate sedimentation occurred during the Oligocene in narrow N-S trending subsident extensional grabens associated with the N-S trending Barjols Triassic uplift

Cancer incidence in British vegetarians

Background: Few prospective studies have examined cancer incidence among vegetarians. Methods: We studied 61 566 British men and women, comprising 32 403 meat eaters, 8562 non-meat eaters who did eat fish ('fish eaters') and 20 601 vegetarians. After an average follow-up of 12.2 years, there were 3350 incident cancers of which 2204 were among meat eaters, 317 among fish eaters and 829 among vegetarians. Relative risks (RRs) were estimated by Cox regression, stratified by sex and recruitment protocol and adjusted for age, smoking, alcohol, body mass index, physical activity level and, for women only, parity and oral contraceptive use. Results: There was significant heterogeneity in cancer risk between groups for the following four cancer sites: stomach cancer, RRs (compared with meat eaters) of 0.29 (95% CI: 0.07–1.20) in fish eaters and 0.36 (0.16–0.78) in vegetarians, P for heterogeneity=0.007; ovarian cancer, RRs of 0.37 (0.18–0.77) in fish eaters and 0.69 (0.45–1.07) in vegetarians, P for heterogeneity=0.007; bladder cancer, RRs of 0.81 (0.36–1.81) in fish eaters and 0.47 (0.25–0.89) in vegetarians, P for heterogeneity=0.05; and cancers of the lymphatic and haematopoietic tissues, RRs of 0.85 (0.56–1.29) in fish eaters and 0.55 (0.39–0.78) in vegetarians, P for heterogeneity=0.002. The RRs for all malignant neoplasms were 0.82 (0.73–0.93) in fish eaters and 0.88 (0.81–0.96) in vegetarians (P for heterogeneity=0.001). Conclusion: The incidence of some cancers may be lower in fish eaters and vegetarians than in meat eaters

Study protocol : the empirical investigation of methods to correct for measurement error in biobanks with dietary assessment

Peer reviewedPublisher PD

LINE-1 methylation in leukocyte DNA, interaction with phosphatidylethanolamine N-methyltransferase variants and bladder cancer risk

BACKGROUND: Aberrant global DNA methylation is shown to increase cancer risk. LINE-1 has been proven a measure of global DNA methylation. The objectives of this study were to assess the association between LINE-1 methylation level and bladder cancer risk and to evaluate effect modification by environmental and genetic factors. METHODS: Bisulphite-treated leukocyte DNA from 952 cases and 892 hospital controls was used to measure LINE-1 methylation level at four CpG sites by pyrosequencing. Logistic regression model was fitted to estimate odds ratios (ORs) and 95% confidence intervals (95% CIs). Interactions between LINE-1 methylation levels and environmental and genetic factors were assessed. RESULTS: The risk of bladder cancer followed a nonlinear association with LINE-1 methylation. Compared with subjects in the middle tertile, the adjusted OR for subjects in the lower and the higher tertiles were 1.26 (95% CI 0.99–1.60, P=0.06) and 1.33 (95% CI 1.05–1.69, P=0.02), respectively. This association significantly increased among individuals homozygous for the major allele of five single-nucleotide polymorphisms located in the phosphatidylethanolamine N-methyltransferase gene (corrected P-interaction<0.05). CONCLUSIONS: The findings from this large-scale study suggest that both low and high levels of global DNA methylation are associated with the risk of bladder cancer

Epigenome-wide association study reveals decreased average methylation levels years before breast cancer diagnosis

Interest in the potential of DNA methylation in peripheral blood as a biomarker of cancer risk is increasing. We aimed to assess whether epigenome-wide DNA methylation measured in peripheral blood samples obtained before onset of the disease is associated with increased risk of breast cancer. We report on three independent prospective nested case-control studies from the European Prospective Investigation into Cancer and Nutrition (EPIC-Italy; n = 162 matched case-control pairs), the Norwegian Women and Cancer study (NOWAC; n = 168 matched pairs), and the Breakthrough Generations Study (BGS; n = 548 matched pairs). We used the Illumina 450k array to measure methylation in the EPIC and NOWAC cohorts. Whole-genome bisulphite sequencing (WGBS) was performed on the BGS cohort using pooled DNA samples, combined to reach 50× coverage across ~16 million CpG sites in the genome including 450k array CpG sites. Mean β values over all probes were calculated as a measurement for epigenome-wide methylation

Geographic factors associated with poorer outcomes in patients diagnosed with covid-19 in primary health care

Background: The prognosis of older age COVID-19 patients with comorbidities is associated with a more severe course and higher fatality rates but no analysis has yet included factors related to the geographical area/municipality in which the affected patients live, so the objective of this study was to analyse the prognosis of patients with COVID-19 in terms of sex, age, comorbidi-ties, and geographic variables. Methods: A retrospective cohort of 6286 patients diagnosed with COVID-19 was analysed, considering demographic data, previous comorbidities and geographic variables. The main study variables were hospital admission, intensive care unit (ICU) admission and death due to worsening symptoms; and the secondary variables were sex, age, comorbidities and geographic variables (size of the area of residence, distance to the hospital and the driving time to the hospital). A comparison analysis and a multivariate Cox model were performed. Results: The multivariate Cox model showed that women had a better prognosis in any type of analysed prog-nosis. Most of the comorbidities studied were related to a poorer prognosis except for dementia, which is related to lower admissions and higher mortality. Suburban areas were associated with greater mortality and with less hospital or ICU admission. Distance to the hospital was also associated with hospital admission. Conclusions: Factors such as type of municipality and distance to hospital act as social health determinants. This fact must be taken account in order to stablish specifics prevention measures and treatment protocols

Clayey materials from the Sierra de la Demanda Range (Spain): their potential as raw materials for the building ceramics industry

This work describes the possible use of thick Early Cretaceous clay deposits, which occur in the southern sector of the Sierra de la Demanda range, as raw materials in the manufacture of ceramic articles. The global mineralogical composition is characterized by high proportions of phyllosilicates and quartz with variable quantities of feldspars, carbonates and hematite. The clay mineralogy differentiates two types of raw materials: illitic clay and kaolinitic-illitic clay. A granulometric distribution in the 2ÿ60 mm fraction, good behaviour during the drying stage and acceptable results in firing tests confirmed that most samples can be utilized as raw material in the building ceramics industry. The range of suitable firing temperatures for these materials is 950ÿ1000ëC, a temperature which needs to be raised for samples with a high percentage of kaolinite and quartz. Moreover, other materials with abundant calcite (20ÿ30%) are suitable for use as modifiers of some properties or colour

Satellite‐Based Monitoring of Irrigation Water Use: Assessing Measurement Errors and Their Implications for Agricultural Water Management Policy

Reliable accounting of agricultural water use is critical for sustainable water management. However, the majority of agricultural water use is not monitored, with limited metering of irrigation despite increasing pressure on both groundwater and surface water resources in many agricultural regions worldwide. Satellite remote sensing has been proposed as a low-cost and scalable solution to fill widespread gaps in monitoring of irrigation water use in both developed and developing countries, bypassing the technical, socioeconomic, and political challenges that to date have constrained in situ metering. In this paper, we show through a systematic meta-analysis that the relative accuracy of different satellite-based irrigation water use monitoring approaches remains poorly understood, with evidence of large uncertainties when water use estimates are validated against in situ irrigation data at both field and regional scales. Subsequently, we demonstrate that water use measurement errors result in large economic welfare losses for farmers and may negatively impact ability of policies to limit acute and nonlinear externalities of irrigation abstraction on both the environment and other water users. Our findings highlight that water resource planners must consider the trade-offs between accuracy and costs associated with different water use accounting approaches. Remote sensing has an important role to play in supporting improved agricultural water accounting—both independently and in combination with in situ monitoring. However, greater transparency and evidence is needed about underlying uncertainties in satellite-based models, along with how these measurement errors affect the performance of associated policies to manage different short- and long-term externalities of irrigation water use