Complete bilayer adsorption of C16TAB on the surface of mica using neutron reflection.

We present neutron reflection data from an alkylammonium surfactant (C16TAB) at the mica/water interface. The system is studied in situ in a noninvasive manner and indicates the formation of a complete adsorbed bilayer with little evidence of defects. A detailed analysis suggests that the data are not consistent with some other previously reported adsorbed structures, such as micelles or cylinders.BPThis is the accepted manuscript. The final version is available at http://pubs.acs.org/doi/full/10.1021/acs.jpcb.5b00397

Survival of Mycobacterium abscessus isolated from people with CF in artificially generated aerosols

M. abscessus survives aerosolisation suggesting airborne transmission between people with CF may occu

Pronouncing French Names in New Orleans

This article, based on 984 interviews with bearers of French names in the city of New Orleans, investigates the use of the notion of pronunciation as a device by which speakers manage their talk. The investigation proceeded primarily by eliciting ways in which people employ devices for talking about talk in everyday communicative interactions, as a means to manage various types of communicational phenomena and to deal with communication difficulties emerging from a clash of phonetic traditions. The result is a definition of pronunciation in terms which are used by a majority of speakers. An appendix gives a list of names, with comments by their bearers concerning ways in which those bearers would attempt to convey to mispronouncers the correct pronunciation of their names

In situ neutron scattering of antibody adsorption during protein A chromatography

A deeper understanding of the nanoscale and mesoscale structure of chromatographic adsorbents and the distribution of proteins within the media, is critical to a mechanistic understanding of separation processes using these materials. Characterisation of the media's architecture at this scale and protein adsorption within, is challenging using conventional techniques. In this study, we propose a novel resin characterisation technique that enables in-situ measurement of the structure of the adsorbed protein layer within the resin, under typical chromatographic conditions. A quartz flow-through cell was designed and fabricated for use with Small Angle Neutron Scattering (SANS), in order to measure the nanoscale to mesoscale structures of a silica based protein A chromatography resin during the monoclonal antibody sorption process. We were able to examine the pore-to-pore (˜133 nm) and pore size (˜63 nm) correlations of the resin and the in-plane adsorbed antibody molecules (˜ 4.2 nm) correlation at different protein loadings and washing buffers, in real time using a contrast matching approach. When 0.03 M sodium phosphate with 1 M urea and 10 % isopropanol buffer, pH 8, was introduced into the system as a wash buffer, it disrupted the system's order by causing partial unfolding of the adsorbed antibody, as evidenced by a loss of the in-plane protein correlation. This method offers new ways to investigate the nanoscale structure and ligand immobilisation within chromatography resins; and perhaps most importantly understand the in-situ behaviour of adsorbed proteins within the media under different mobile phase conditions within a sample environment replicating that of a chromatography column

The Problem of Contextuality and the Impossibility of Experimental Metaphysics Thereof

Recently a new impulse has been given to the experimental investigation of contextuality. In this paper we show that for a widely used definition of contextuality there can be no decisive experiment on the existence of contextuality. To this end, we give a clear presentation of the hidden variable models due to Meyer, Kent and Clifton (MKC), which would supposedly nullify the Kochen-Specker Theorem. Although we disagree with this last statement, the models play a significant role in the discussion on the meaning of contextuality. In fact, we introduce a specific MKC-model of which we show that it is non-contextual and completely in agreement with quantum mechanical predictions. We also investigate the possibility of other definitions of non-contextuality --with an emphasis on operational definitions-- and argue that any useful definition relies on the specification of a theoretical framework. It is therefore concluded that no experimental test can yield any conclusions about contextuality on a metaphysical level

Cosmological Scaling Solutions with Tachyon:Modified Gravity Model

Modifying the Einstein's gravity at large distance scales is one of the interesting proposals to explain the late time acceleration of the universe. In this paper, we analyse scaling solutions in modified gravity models where the universe is sourced by a background matter fluid together with a tachyon type scalar field. We describe a general prescription to calculate the scaling potential in such models. Later on, we consider specific examples of modifications and apply our method to calculate the scaling potential and the scale factor. Our method can be applied to any modified gravity model, in presence of a tachyon field.Comment: 6 pages, latex style, modified version, two new figures included, new refernces added, Accepted for publication in Physics Letters

Neutron reflectivity measurement of protein A-antibody complex at the solid-liquid interface

Chromatography is a ubiquitous unit operation in the purification of biopharmaceuticals yet few studies have addressed the biophysical characterisation of proteins at the solution-resin interface. Chromatography and other adsorption and desorption processes have been shown to induce protein aggregation which is undesirable in biopharmaceutical products. In order to advance understanding of how adsorption processes might impact protein stability, neutron reflectivity was used to characterise the structure of adsorbed immunoglobulin G (IgG) on model surfaces. In the first model system, IgG was adsorbed directly to silica and demonstrated a side-on orientation with high surface contact. A maximum dimension of 60Å in the surface normal direction and high density surface coverage were observed under pH 4.1 conditions. In chromatography buffers, pH was found to influence IgG packing density and orientation at the solid-liquid interface. In the second model system, which was designed to mimic an affinity chromatography surface, protein A was attached to a silica surface to produce a configuration representative of a porous glass chromatography resin. Interfacial structure was probed during sequential stages from ligand attachment, through to IgG binding and elution. Adsorbed IgG structures extended up to 250Å away from the surface and showed dependence on surface blocking strategies. The data was suggestive of two IgG molecules bound to protein A with a somewhat skewed orientation and close proximity to the silica surface. The findings provide insight into the orientation of adsorbed antibody structures under conditions encountered during chromatographic separations

Herd-level risk factors of bovine tuberculosis in England and Wales after the 2001 foot-and-mouth disease epidemic

We present the results of a 2005 case–control study of bovine tuberculosis (bTB) breakdowns in English and Welsh herds. The herd management, farming practices, and environmental factors of 401matched pairs of case and control herds were investigated to provide a picture of herd-level risk factors in areas of varying bTB incidence. A global conditional logistic regression model, with region-specific variants, was used to compare case herds that had experienced a confirmed bTB breakdown to contemporaneous control herds matched on region, herd type, herd size, and parish testing interval. Contacts with cattle from contiguous herds and sourcing cattle from herds with a recent history of bTB were associated with an increased risk in both the global and regional analyses. Operating a farm over several premises, providing cattle feed inside the housing, and the presence of badgers were also identified as significantly associated with an increased bTB risk. Steps taken to minimize cattle contacts with neighboring herds and altering trading practices could have the potential to reduce the size of the bTB epidemic. In principle, limiting the interactions between cattle and wildlife may also be useful; however this study did not highlight any specific measures to implement

Quantum singularities in a model of f(R) Gravity

The formation of a naked singularity in a model of f(R) gravity having as source a linear electromagnetic field is considered in view of quantum mechanics. Quantum test fields obeying the Klein-Gordon, Dirac and Maxwell equations are used to probe the classical timelike naked singularity developed at r=0. We prove that the spatial derivative operator of the fields fails to be essentially self-adjoint. As a result, the classical timelike naked singularity remains quantum mechanically singular when it is probed with quantum fields having different spin structures.Comment: 12 pages, final version. Accepted for publication in EPJ