240 research outputs found
Clover:cereal bi-cropping for organic farms (OF0173)
This is the final report of Defra project OF0173. The attached main report starts with a more detailed summary, from which this text is extracted.
IGER, IACR and others developed a system for growing cereals, especially for whole crop silage, that enables either greatly reduced or zero levels of N fertilizer and agrochemicals to be used. The system is simple and straightforward and relies on direct drilling of the cereal into an established understorey of white clover. The white clover understorey becomes permanent and perennial and successive cereal crops are drilled into it, harvested and re-drilled each year. The clover provides N for the cereal crop and the gross changes in crop architecture that occur (a) encourage large populations of predatory invertebrates that devour pest species and hence obviate the need for insecticide use (b) frustrate the spread of fungal foliar diseases and remove the need to use fungicides and (c) suppress most weeds.
Some of the advantages of the clover:cereal bi-cropping system that are especially relevant to organic farms are
a) simultaneous cropping and fertility building rather than separate seasons for each
b) effective nutrient cycling
c) weeds replaced by clover; limitation of crop area requiring intensive weed control
d) confusion of insect pests and habitat for beneficial insects, spiders etc.
e) improved field access relative to bare soil
The work investigated the agronomic viability and sustainability of bi-cropping for both silage and grain production in organic farming systems as specified under UKROFS. Experiments were carried out on three UKROF approved sites with contrasting soil types and environmental conditions. Randomised block, small-plot experiments evaluated the most satisfactory ways of modifying the IGER/IACR system for organic farming.
The main point to emerge is that although the bi-crop system is well proven and developed for use in a non-organic system, grass weeds remain a problem in translating the system to organic farming as herbicides can not be used to control them. There are a number of strategies that emerged during the course of the present work that indicated ways in which the grass weed problem may be overcome in future. Firstly, oats seem to suppress grass weeds and growing this crop instead of wheat appeared to show considerable promise. Secondly strip drilling alternate 20 cm strips of cereal with 30 cm strips of clover allows separate management of the two crop components, facilitating weed control. The work confirmed that spring-sown cereals are not an option for bi-cropping as they are easily outcompeted by the clover.
An important positive feature of all of the trials was the consistent absence or low levels of diseases and pests despite high levels of airborne pathogen inoculum in the trial area. It is difficult to gauge the relative contributions of the organic system as a whole and the bi-crop system in particular to this feature. There are certainly important theoretical reasons as to why the bi-crop system should help in this direction, including the restriction on spread of splash-borne diseases because of the presence of clover around the cereal plants, green background confusion of insect pests and the probable lack of surplus soluble nitrogen in the cereal plants.
The more positive results in the absence of grass weeds were limited to a single year for cereals (and two seasons for vegetables in another related trial). However, in both cases, there were clear indications that, with a relatively small amount of fine tuning of the system, it should be possible to obtain highly acceptable results from both types of inter-cropping and, indeed, from rotational integration of the two. Further progress would certainly be worthwhile for the organic producer because it seems clear that a modest further adjustment of the competitive balance between crop and clover will lead to a clearer expression of all of the potential advantages of such systems, outlined above. Despite the difficulties encountered, progress was made and the list of advantages and potential advantages of bi-cropping for organic agriculture is so large and significant that further work should be done to capitalise on that completed to date
Regional Endothermy in a Coral Reef Fish?
Although a few pelagic species exhibit regional endothermy, most fish are regarded as ectotherms. However, we document significant regional endothermy in a benthic reef fish. Individual steephead parrotfish, Chlorurus microrhinos (Labridae, formerly Scaridae) were tagged and their internal temperatures were monitored for a 24 h period using active acoustic telemetry. At night, on the reef, C. microrhinos were found to maintain a consistent average peritoneal cavity temperature 0.16Β±0.005Β°C (SE) warmer than ambient. Diurnal internal temperatures were highly variable for individuals monitored on the reef, while in tank-based trials, peritoneal cavity temperatures tracked environmental temperatures. The mechanisms responsible for a departure of the peritoneal cavity temperature from environmental temperature occurred in C. microrhinos are not yet understood. However, the diet and behavior of the species suggests that heat in the peritoneal cavity may result primarily from endogenous thermogenesis coupled with physiological heat retention mechanisms. The presence of limited endothermy in C. microrhinos indicates that a degree of uncertainty may exist in the manner that reef fish respond to their thermal environment. At the very least, they do not always appear to respond to environmental temperatures as neutral thermal vessels and do display limited, but significant, visceral warming
Ecological influences on the behaviour and fertility of malaria parasites
BACKGROUND: Sexual reproduction in the mosquito is essential for the transmission of malaria parasites and a major target for transmission-blocking interventions. Male gametes need to locate and fertilize females in the challenging environment of the mosquito blood meal, but remarkably little is known about the ecology and behaviour of male gametes. METHODS: Here, a series of experiments explores how some aspects of the chemical and physical environment experienced during mating impacts upon the production, motility, and fertility of male gametes. RESULTS AND CONCLUSIONS: Specifically, the data confirm that: (a) rates of male gametogenesis vary when induced by the family of compounds (tryptophan metabolites) thought to trigger gamete differentiation in nature; and (b) complex relationships between gametogenesis and mating success exist across parasite species. In addition, the data reveal that (c) microparticles of the same size as red blood cells negatively affect mating success; and (d) instead of swimming in random directions, male gametes may be attracted by female gametes. Understanding the mating ecology of malaria parasites, may offer novel approaches for blocking transmission and explain adaptation to different species of mosquito vectors
Determining the neurotransmitter concentration profile at active synapses
Establishing the temporal and concentration profiles of neurotransmitters during synaptic release is an essential step towards understanding the basic properties of inter-neuronal communication in the central nervous system. A variety of ingenious attempts has been made to gain insights into this process, but the general inaccessibility of central synapses, intrinsic limitations of the techniques used, and natural variety of different synaptic environments have hindered a comprehensive description of this fundamental phenomenon. Here, we describe a number of experimental and theoretical findings that has been instrumental for advancing our knowledge of various features of neurotransmitter release, as well as newly developed tools that could overcome some limits of traditional pharmacological approaches and bring new impetus to the description of the complex mechanisms of synaptic transmission
The prevalence of enteroviral capsid protein vp1 immunostaining in pancreatic islets in human type 1 diabetes.
addresses: Institute of Biomedical and Clinical Sciences, Peninsula Medical School, Plymouth, UK.The final publication is available at link.springer.com/article/10.1007%2Fs00125-009-1276-0Evidence that the beta cells of human patients with type 1 diabetes can be infected with enterovirus is accumulating, but it remains unclear whether such infections occur at high frequency and are important in the disease process. We have now assessed the prevalence of enteroviral capsid protein vp1 (vp1) staining in a large cohort of autopsy pancreases of recent-onset type 1 diabetic patients and a range of controls
Utilisation of an operative difficulty grading scale for laparoscopic cholecystectomy
Background
A reliable system for grading operative difficulty of laparoscopic cholecystectomy would standardise description of findings and reporting of outcomes. The aim of this study was to validate a difficulty grading system (Nassar scale), testing its applicability and consistency in two large prospective datasets.
Methods
Patient and disease-related variables and 30-day outcomes were identified in two prospective cholecystectomy databases: the multi-centre prospective cohort of 8820 patients from the recent CholeS Study and the single-surgeon series containing 4089 patients. Operative data and patient outcomes were correlated with Nassar operative difficultly scale, using Kendallβs tau for dichotomous variables, or JonckheereβTerpstra tests for continuous variables. A ROC curve analysis was performed, to quantify the predictive accuracy of the scale for each outcome, with continuous outcomes dichotomised, prior to analysis.
Results
A higher operative difficulty grade was consistently associated with worse outcomes for the patients in both the reference and CholeS cohorts. The median length of stay increased from 0 to 4 days, and the 30-day complication rate from 7.6 to 24.4% as the difficulty grade increased from 1 to 4/5 (both pβ<β0.001). In the CholeS cohort, a higher difficulty grade was found to be most strongly associated with conversion to open and 30-day mortality (AUROCβ=β0.903, 0.822, respectively). On multivariable analysis, the Nassar operative difficultly scale was found to be a significant independent predictor of operative duration, conversion to open surgery, 30-day complications and 30-day reintervention (all pβ<β0.001).
Conclusion
We have shown that an operative difficulty scale can standardise the description of operative findings by multiple grades of surgeons to facilitate audit, training assessment and research. It provides a tool for reporting operative findings, disease severity and technical difficulty and can be utilised in future research to reliably compare outcomes according to case mix and intra-operative difficulty
Pleosporales
One hundred and five generic types of Pleosporales are described and illustrated. A brief introduction and detailed history with short notes on morphology, molecular phylogeny as well as a general conclusion of each genus are provided. For those genera where the type or a representative specimen is unavailable, a brief note is given. Altogether 174 genera of Pleosporales are treated. Phaeotrichaceae as well as Kriegeriella, Zeuctomorpha and Muroia are excluded from Pleosporales. Based on the multigene phylogenetic analysis, the suborder Massarineae is emended to accommodate five families, viz. Lentitheciaceae, Massarinaceae, Montagnulaceae, Morosphaeriaceae and Trematosphaeriaceae
Regulatory potential for concerted modulation of Nrf2- and Nfkb1-mediated gene expression in inflammation and carcinogenesis
Many studies have implicated nuclear factor E2-related factor 2 (Nrf2) and nuclear factor-ΞΊB1 (Nfkb1) in inflammation and cancer. However, the regulatory potential for crosstalk between these two important transcription factors in inflammation and carcinogenesis has not been explored. To delineate conserved transcription factor-binding site signatures, we performed bioinformatic analyses on the promoter regions of human and murine Nrf2 and Nfkb1. We performed multiple sequence alignment of Nrf2 and Nfkb1 genes in five mammalian species β human, chimpanzee, dog, mouse and rat β to explore conserved biological features. We constructed a canonical regulatory network for concerted modulation of Nrf2 and Nfkb1 involving several members of the mitogen-activated protein kinase (MAPK) family and present a putative model for concerted modulation of Nrf2 and Nfkb1 in inflammation/carcinogenesis. Our results reflect potential for putative crosstalk between Nrf2 and Nfkb1 modulated through the MAPK cascade that may influence inflammation-associated etiopathogenesis of cancer. Taken together, the elucidation of potential relationships between Nrf2 and Nfkb1 may help to better understand transcriptional regulation, as well as transcription factor networks, associated with the etiopathogenesis of inflammation and cancer
Comparative Phylogeography of a Coevolved Community: Concerted Population Expansions in Joshua Trees and Four Yucca Moths
Comparative phylogeographic studies have had mixed success in identifying common phylogeographic patterns among co-distributed organisms. Whereas some have found broadly similar patterns across a diverse array of taxa, others have found that the histories of different species are more idiosyncratic than congruent. The variation in the results of comparative phylogeographic studies could indicate that the extent to which sympatrically-distributed organisms share common biogeographic histories varies depending on the strength and specificity of ecological interactions between them. To test this hypothesis, we examined demographic and phylogeographic patterns in a highly specialized, coevolved community β Joshua trees (Yucca brevifolia) and their associated yucca moths. This tightly-integrated, mutually interdependent community is known to have experienced significant range changes at the end of the last glacial period, so there is a strong a priori expectation that these organisms will show common signatures of demographic and distributional changes over time. Using a database of >5000 GPS records for Joshua trees, and multi-locus DNA sequence data from the Joshua tree and four species of yucca moth, we combined paleaodistribution modeling with coalescent-based analyses of demographic and phylgeographic history. We extensively evaluated the power of our methods to infer past population size and distributional changes by evaluating the effect of different inference procedures on our results, comparing our palaeodistribution models to Pleistocene-aged packrat midden records, and simulating DNA sequence data under a variety of alternative demographic histories. Together the results indicate that these organisms have shared a common history of population expansion, and that these expansions were broadly coincident in time. However, contrary to our expectations, none of our analyses indicated significant range or population size reductions at the end of the last glacial period, and the inferred demographic changes substantially predate Holocene climate changes
Mental health of victims of sexual violence in eastern Congo: associations with daily stressors, stigma, and labeling
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