Changes in intracellular ion activities induced by adrenaline in human and rat skeletal muscle

To study the stimulating effect of adrenaline (ADR) on active Na+/K+ transport we used double-barrelled ion-sensitive micro-electrodes to measure the activities of extracellular K+ (aKe) and intracellular Na+ (aNai) in isolated preparations of rat soleus muscle, normal human intercostal muscle and one case of hyperkalemic periodic paralysis (h.p.p.). In these preparations bath-application of ADR (10−6 M) resulted in a membrane hyperpolarization and transient decreasesaKe andaNai which could be blocked by ouabain (3×10−4 M). In the h.p.p. muslce a continuous rise ofaNai induced by elevation ofaKe to 5.2 mM could be stopped by ADR. In addition, the intracellular K+ activity (aKi), the free intracellular Ca2+ concentration (pCai) and intracellular pH (pHi) were monitored in rat soleus muscle. During ADRaKi increased, pHi remained constant and intracellular Ca2+ apparently decreased. In conclusion, our data show that ADR primarily stimulates the Na+/K+ pump in mammalian skeletal muscle. This stimulating action is not impaired in the h.p.p. muscle

Absolute dimensions of eclipsing binaries. XXVIII. BK Pegasi and other F-type binaries: Prospects for calibration of convective core overshoot

We present a detailed study of the F-type detached eclipsing binary BK Peg, based on new photometric and spectroscopic observations. The two components, which have evolved to the upper half of the main-sequence band, are quite different with masses and radii of (1.414 +/- 0.007 Msun, 1.988 +/- 0.008 Rsun) and (1.257 +/- 0.005 Msun, 1.474 +/- 0.017 Rsun), respectively. The 5.49 day period orbit of BK Peg is slightly eccentric (e = 0.053). The measured rotational velocities are 16.6 +/- 0.2 (primary) and 13.4 +/- 0.2 (secondary) km/s. For the secondary component this corresponds to (pseudo)synchronous rotation, whereas the primary component seems to rotate at a slightly lower rate. We derive an iron abundance of [Fe/H] =-0.12 +/- 0.07 and similar abundances for Si, Ca, Sc, Ti, Cr and Ni. Yonsei-Yale and Victoria-Regina evolutionary models for the observed metal abundance reproduce BK Peg at ages of 2.75 and 2.50 Gyr, respectively, but tend to predict a lower age for the more massive primary component than for the secondary. We find the same age trend for three other upper main-sequence systems in a sample of well studied eclipsing binaries with components in the 1.15-1.70 Msun range, where convective core overshoot is gradually ramped up in the models. We also find that the Yonsei-Yale models systematically predict higher ages than the Victoria-Regina models. The sample includes BW Aqr, and as a supplement we have determined a [Fe/H] abundance of -0.07 +/- 0.11 for this late F-type binary. We propose to use BK Peg, BW Aqr, and other well-studied 1.15-1.70 Msun eclipsing binaries to fine-tune convective core overshoot, diffusion, and possibly other ingredients of modern theoretical evolutionary models.Comment: Accepted for publication in Astronomy and Astrophysic

Generation of arbitrary quantum states of traveling fields

We show that any single-mode quantum state can be generated from the vacuum by alternate application of the coherent displacement operator and the creation operator. We propose an experimental implementation of the scheme for traveling optical fields, which is based on field mixings and conditional measurements in a beam splitter array, and calculate the probability of state generation.Comment: 1 Table and 2 Postscript figures, using Latex; modifications and changes in Figure 2, Table 1 and Eqs. 11-13,17,18,2

Dragging a polymer chain into a nanotube and subsequent release

We present a scaling theory and Monte Carlo (MC) simulation results for a flexible polymer chain slowly dragged by one end into a nanotube. We also describe the situation when the completely confined chain is released and gradually leaves the tube. MC simulations were performed for a self-avoiding lattice model with a biased chain growth algorithm, the pruned-enriched Rosenbluth method. The nanotube is a long channel opened at one end and its diameter $D$ is much smaller than the size of the polymer coil in solution. We analyze the following characteristics as functions of the chain end position $x$ inside the tube: the free energy of confinement, the average end-to-end distance, the average number of imprisoned monomers, and the average stretching of the confined part of the chain for various values of $D$ and for the number of monomers in the chain, $N$. We show that when the chain end is dragged by a certain critical distance $x^*$ into the tube, the polymer undergoes a first-order phase transition whereby the remaining free tail is abruptly sucked into the tube. This is accompanied by jumps in the average size, the number of imprisoned segments, and in the average stretching parameter. The critical distance scales as $x^*\sim ND^{1-1/\nu}$. The transition takes place when approximately 3/4 of the chain units are dragged into the tube. The theory presented is based on constructing the Landau free energy as a function of an order parameter that provides a complete description of equilibrium and metastable states. We argue that if the trapped chain is released with all monomers allowed to fluctuate, the reverse process in which the chain leaves the confinement occurs smoothly without any jumps. Finally, we apply the theory to estimate the lifetime of confined DNA in metastable states in nanotubes.Comment: 13pages, 14figure