Radio observations of the massive stellar cluster Westerlund 1

High-dynamic range radio observations of Westerlund 1 are presented that detect a total of 21 stars in the young massive stellar cluster, the richest population of radio emitting stars known for any young massive galactic cluster in the Galaxy. We will discuss some of the more remarkable objects, including the highly radio luminous supergiant B[e] star W9, with an estimated mass-loss rate ~10^{-3} solarmass/yr, comparable to that of eta Carina, along with the somewhat unusual detection of thermal emission from almost all the cool red supergiants and yellow hypergiants. There is strong supporting evidence from X-ray observations that each of the WR stars with radio emission are likely to be colliding-wind binariesComment: To appear in the proceedings of "Massive Stars: Fundamental Parameters and Circumstellar Interactions". 2 pages, 1 figur

Spectroscopy and 3D imaging of the Crab nebula

Spectroscopy of the Crab nebula along different slit directions reveals the 3 dimensional structure of the optical nebula. On the basis of the linear radial expansion result first discovered by Trimble (1968), we make a 3D model of the optical emission. Results from a limited number of slit directions suggest that optical lines originate from a complicated array of wisps that are located in a rather thin shell, pierced by a jet. The jet is certainly not prominent in optical emission lines, but the direction of the piercing is consistent with the direction of the X-ray and radio jet. The shell's effective radius is ~ 79 seconds of arc, its thickness about a third of the radius and it is moving out with an average velocity 1160 km/s.Comment: 21 pages, 14 figures, submitted to ApJ, 3D movie of the Crab nebula available at http://www.fiz.uni-lj.si/~vidrih

Structural basis for complement factor H-linked age-related macular degeneration

This is the final version of the article. Available from the publisher via the DOI in this record.Nearly 50 million people worldwide suffer from age-related macular degeneration (AMD), which causes severe loss of central vision. A single-nucleotide polymorphism in the gene for the complement regulator factor H (FH), which causes a Tyr-to-His substitution at position 402, is linked to approximately 50% of attributable risks for AMD. We present the crystal structure of the region of FH containing the polymorphic amino acid His402 in complex with an analogue of the glycosaminoglycans (GAGs) that localize the complement regulator on the cell surface. The structure demonstrates direct coordination of ligand by the disease-associated polymorphic residue, providing a molecular explanation of the genetic observation. This glycan-binding site occupies the center of an extended interaction groove on the regulator's surface, implying multivalent binding of sulfated GAGs. This finding is confirmed by structure-based site-directed mutagenesis, nuclear magnetic resonance-monitored binding experiments performed for both H402 and Y402 variants with this and another model GAG, and analysis of an extended GAG-FH complex.B. Prosser is funded by the Wellcome Trust Structural Biology Training Program (075415/Z/04/Z). S. Johnson and P. Roversi were funded by grants to S.M. Lea from the Medical Research Council (MRC) of the United Kingdom (grants G0400389 and G0400775). D. Uhrin and P.N. Barlow were funded by the Wellcome Trust (078780/ Z/05/Z). S.J. Clark was funded by an MRC Doctoral Training Account (G78/7925), and R.B. Sim and A.J. Day were funded by MRC core funding to the MRC Immunochemistry Unit

A novel liver specific isoform of the rat LAR transcript is expressed as a truncated isoform encoded from a 5'UTR located within intron 11

<p>Abstract</p> <p>Background</p> <p>The leukocyte common antigen related receptor (LAR) protein has been shown to modulate the signal transduction of a number of different growth factors, including insulin and insulin-like growth factor 1. Splice variants exhibit differing roles and are expressed according to tissue type and developmental stage.</p> <p>Results</p> <p>Using 5'RACE, we identified a 5'UTR within intron 11 of the rat LAR gene. We demonstrated that this gives rise to a novel isoform of the LAR transcript encoded from the identified region within intron 11. By priming across the site from exon 11 to exon 15 we show that the novel 5'UTR is not represented in the full-length transcript and thus, it produces a truncated form of the LAR mRNA. We examined the tissue distribution of this novel isoform and found it to be exclusively expressed in liver. We additionally identified a liver specific 150 kDa band with western blotting which we propose may represent the protein product of the novel transcript. Luciferase assays showed the region immediately upstream of the 5'UTR to possesses considerable promoter activity and that this may be conferred by the presence of a number of putative binding sites for liver enriched transcription factors.</p> <p>Conclusion</p> <p>In summary, we describe a novel, liver specific, truncated isoform of the LAR transcript transcribed under the control of an intronic promoter, potentially representing a previously unidentified modulator of hepatic insulin signalling.</p

The role of cosmic ray pressure in accelerating galactic outflows

We study the formation of galactic outflows from supernova explosions (SNe) with the moving-mesh code AREPO in a stratified column of gas with a surface density similar to the Milky Way disk at the solar circle. We compare different simulation models for SNe placement and energy feedback, including cosmic rays (CR), and find that models that place SNe in dense gas and account for CR diffusion are able to drive outflows with similar mass loading as obtained from a random placement of SNe with no CRs. Despite this similarity, CR-driven outflows differ in several other key properties including their overall clumpiness and velocity. Moreover, the forces driving these outflows originate in different sources of pressure, with the CR diffusion model relying on non-thermal pressure gradients to create an outflow driven by internal pressure and the random-placement model depending on kinetic pressure gradients to propel a ballistic outflow. CRs therefore appear to be non-negligible physics in the formation of outflows from the interstellar medium.Comment: 8 pages, 4 figures, accepted for publication in ApJL; movie of simulated gas densities can be found here: http://www.h-its.org/tap-images/galactic-outflows

High-resolution QTL mapping in Tetranychus urticae reveals acaricide-specific responses and common target-site resistance after selection by different METI-I acaricides

Arthropod herbivores cause dramatic crop losses, and frequent pesticide use has led to widespread resistance in numerous species. One such species, the two-spotted spider mite, Tetranychus urticae, is an extreme generalist herbivore and a major worldwide crop pest with a history of rapidly developing resistance to acaricides. Mitochondrial Electron Transport Inhibitors of complex I (METI-Is) have been used extensively in the last 25 years to control T. urticae around the globe, and widespread resistance to each has been documented. METI-I resistance mechanisms in T. urticae are likely complex, as increased metabolism by cytochrome P450 monooxygenases as well as a target-site mutation have been linked with resistance. To identify loci underlying resistance to the METI-I acaricides fenpyroximate, pyridaben and tebufenpyrad without prior hypotheses, we crossed a highly METI-I-resistant strain of T. urticae to a susceptible one, propagated many replicated populations over multiple generations with and without selection by each compound, and performed bulked segregant analysis genetic mapping. Our results showed that while the known H92R target-site mutation was associated with resistance to each compound, a genomic region that included cytochrome P450-reductase (CPR) was associated with resistance to pyridaben and tebufenpyrad. Within CPR, a single nonsynonymous variant distinguished the resistant strain from the sensitive one. Furthermore, a genomic region linked with tebufenpyrad resistance harbored a non-canonical member of the nuclear hormone receptor 96 (NHR96) gene family. This NHR96 gene does not encode a DNA-binding domain (DBD), an uncommon feature in arthropods, and belongs to an expanded family of 47 NHR96 proteins lacking DBDs in T. urticae. Our findings suggest that although cross-resistance to METI-Is involves known detoxification pathways, structural differences in METI-I acaricides have also resulted in resistance mechanisms that are compound-specific

Changes in zircon chemistry during archean UHT metamorphism in the Napier complex, Antarctica

Zircons from two paragneisses (from Mount Sones and Dallwitz Nunatak) and one orthogneiss (from Gage Ridge) in the Tula Mountains, Napier Complex (East Antarctica) were analyzed for U-Pb age, oxygen isotopes, REEs and by scanning ion imaging. A large number of zircons from all samples are reversely discordant. Mount Sones zircons show an age range from 3.0 Ga to 2.5 Ga and underwent high-grade metamorphism at both ~2.8 Ga and 2.5 Ga. Zircons from Dallwitz Nunatak record detrital ages between 3.5 Ga and 2.5 Ga. Zircons from Gage Ridge record multiple age groups, with concordant data between 3.6 Ga and 3.3 Ga and reversely discordant data that form a discrete ~3.8 Ga population. All of the grains show evidence of Pb mobility during metamorphism. Ion imaging of zircons reveals Y and U zonation, characteristic of magmatic zircon, together with a micro-scale patchy distribution of 206Pb and 207Pb that does not correspond to either growth zonation or crystal imperfections. Some of these patches yield 207Pb/206Pb ages >4 Ga, whereas others yield ages younger than the magmatic crystallization age. Reversely discordant data are the result of ancient Pb mobilization, which is independent of the degree of metamictisation, oxygen isotope and REE content of the zircons. This mobilization can result in spurious ages and was most likely caused by polymetamorphism under anhydrous conditions; that is two high-grade events; one poorly defined at ~2.8 Ga and the other ultra-high temperature (UHT) metamorphism at 2.5 Ga

The Arches cluster revisited

The Arches is one of the youngest, densest and most massive clusters in the Galaxy. As such it provides a unique insight into the lifecycle of the most massive stars known and the formation and survival of such stellar aggregates in the extreme conditions of the Galactic Centre. In a previous study we presented an initial stellar census for the Arches and in this work we expand upon this, providing new and revised classifications for ∼30% of the 105 spectroscopically identified cluster members as well as distinguishing potential massive runaways. The results of this survey emphasise the homogeneity and co-evality of the Arches and confirm the absence of H-free Wolf-Rayets of WC sub-type and predicted luminosities. The increased depth of our complete dataset also provides significantly better constraints on the main sequence population; with the identification of O9.5 V stars for the first time we now spectroscopically sample stars with initial masses ranging from ∼16 M⊙ to ≥120 M⊙. Indeed, following from our expanded stellar census we might expect ≳50 stars within the Arches to have been born with masses ≳60 M⊙, while all 105 spectroscopically confirmed cluster members are massive enough to leave relativistic remnants upon their demise. Moreover the well defined observational properties of the main sequence cohort will be critical to the construction of an extinction law appropriate for the Galactic Centre and consequently the quantitative analysis of the Arches population and subsequent determination of the cluster initial mass function

Massive Stars in Molecular Clouds Rich in High-energy Sources: The Bridge of G332.809-0.132 and CS 78 in NGC 6334

Detections of massive stars in the direction of the H II region CS 78 in NGC 6334 and of G332.809-0.132 are here presented. The region covered by the G332.809-0.132 complex coincides with the RCW 103 stellar association. In its core (40' in radius), approximately 110 OB candidate stars (Ks < 10 mag and 0.4 < AKs < 1.6 mag) were identified using 2MASS, DENIS, and GLIMPSE data. This number of OB stars accounts for more than 50% of the observed number of Lyman continuum photons from this region. Medium-resolution K-band spectra were obtained for seven early types, including one WN 8 star and one Ofpe/WN 9 star; the latter is located near the RCW 103 remnant and its luminosity is consistent with a distance of about 3 kpc. The area analyzed encloses 9 of the 34 OB stars previously known in RCW 103, as well as IRAS 16115-5044, which we reclassify as a candidate luminous blue variable. The line of sight is particularly interesting, crossing three spiral arms; a molecular cloud at -50 (with RCW 103 in the Scutum-Crux arm) and another at -90 km s-1 (in the Norma arm) are detected, both rich in massive stars and supernova remnants. We also report the detection of a B supergiant as the main ionizing source of CS 78, 2MASS J17213513-3532415. Medium-resolution H and K band spectra display H I and He I lines, as well as Fe II lines. By assuming a distance of 1.35 kpc, we estimate a bolometric magnitude of -6.16, which is typical of supergiants.Comment: 44 pages, 15 figure

The potential for dietary factors to prevent or treat osteoarthritis

Osteoarthritis (OA) is a degenerative joint disease for which there are no disease-modifying drugs. It is a leading cause of disability in the UK. Increasing age and obesity are both major risk factors for OA and the health and economic burden of this disease will increase in the future. Focusing on compounds from the habitual diet that may prevent the onset or slow the progression of OA is a strategy that has been under-investigated to date. An approach that relies on dietary modification is clearly attractive in terms of risk/benefit and more likely to be implementable at the population level. However, before undertaking a full clinical trial to examine potential efficacy, detailed molecular studies are required in order to optimise the design. This review focuses on potential dietary factors that may reduce the risk or progression of OA, including micronutrients, fatty acids, flavonoids and other phytochemicals. It therefore ignores data coming from classical inflammatory arthritides and nutraceuticals such as glucosamine and chondroitin. In conclusion, diet offers a route by which the health of the joint can be protected and OA incidence or progression decreased. In a chronic disease, with risk factors increasing in the population and with no pharmaceutical cure, an understanding of this will be crucial