A bio-inspired image coder with temporal scalability

We present a novel bio-inspired and dynamic coding scheme for static images. Our coder aims at reproducing the main steps of the visual stimulus processing in the mammalian retina taking into account its time behavior. The main novelty of this work is to show how to exploit the time behavior of the retina cells to ensure, in a simple way, scalability and bit allocation. To do so, our main source of inspiration will be the biologically plausible retina model called Virtual Retina. Following a similar structure, our model has two stages. The first stage is an image transform which is performed by the outer layers in the retina. Here it is modelled by filtering the image with a bank of difference of Gaussians with time-delays. The second stage is a time-dependent analog-to-digital conversion which is performed by the inner layers in the retina. Thanks to its conception, our coder enables scalability and bit allocation across time. Also, our decoded images do not show annoying artefacts such as ringing and block effects. As a whole, this article shows how to capture the main properties of a biological system, here the retina, in order to design a new efficient coder.Comment: 12 pages; Advanced Concepts for Intelligent Vision Systems (ACIVS 2011

A novel 2D image compression algorithm based on two levels DWT and DCT transforms with enhanced minimize-matrix-size algorithm for high resolution structured light 3D surface reconstruction

Image compression techniques are widely used in 2D and 3D image and video sequences. There are many types of compression techniques and among the most popular are JPEG and JPEG2000. In this research, we introduce a new compression method based on applying a two level Discrete Wavelet Transform (DWT) and a two level Discrete Cosine Transform (DCT) in connection with novel compression steps for high-resolution images. The proposed image compression algorithm consists of 4 steps: 1) Transform an image by a two level DWT followed by a DCT to produce two matrices: DC- and AC-Matrix, or low and high frequency matrix respectively; 2) apply a second level DCT to the DC-Matrix to generate two arrays, namely nonzero-array and zero-array; 3) apply the Minimize-Matrix-Size (MMS) algorithm to the AC-Matrix and to the other high-frequencies generated by the second level DWT; 4) apply arithmetic coding to the output of previous steps. A novel Fast-Match-Search (FMS) decompression algorithm is used to reconstruct all high-frequency matrices. The FMS-algorithm computes all compressed data probabilities by using a table of data, and then using a binary search algorithm for finding decompressed data inside the table. Thereafter, all decoded DC-values with the decoded AC-coefficients are combined into one matrix followed by inverse two level DCT with two level DWT. The technique is tested by compression and reconstruction of 3D surface patches. Additionally, this technique is compared with JPEG and JPEG2000 algorithm through 2D and 3D RMSE following reconstruction. The results demonstrate that the proposed compression method has better visual properties than JPEG and JPEG2000 and is able to more accurately reconstruct surface patches in 3D

A novel image compression algorithm for high resolution 3D reconstruction

This research presents a novel algorithm to compress high-resolution images for accurate structured light 3D reconstruction. Structured light images contain a pattern of light and shadows projected on the surface of the object, which are captured by the sensor at very high resolutions. Our algorithm is concerned with compressing such images to a high degree with minimum loss without adversely affecting 3D reconstruction. The Compression Algorithm starts with a single level discrete wavelet transform (DWT) for decomposing an image into four sub-bands. The sub-band LL is transformed by DCT yielding a DC-matrix and an AC-matrix. The Minimize-Matrix-Size Algorithm is used to compress the AC-matrix while a DWT is applied again to the DC-matrix resulting in LL2, HL2, LH2 and HH2 sub-bands. The LL2 sub-band is transformed by DCT, while the Minimize-Matrix-Size Algorithm is applied to the other sub-bands. The proposed algorithm has been tested with images of different sizes within a 3D reconstruction scenario. The algorithm is demonstrated to be more effective than JPEG2000 and JPEG concerning higher compression rates with equivalent perceived quality and the ability to more accurately reconstruct the 3D models

Molecular basis of association of receptor activity-modifying protein 3 with the family B G protein-coupled secretin receptor

The three receptor activity-modifying proteins (RAMPs) have been recognized as being important for the trafficking and function of a subset of family B G protein-coupled receptors, although the structural basis for this has not been well established. In the current work, we use morphological fluorescence techniques, bioluminescence resonance energy transfer, and bimolecular fluorescence complementation to demonstrate that the secretin receptor associates specifically with RAMP3, but not with RAMP1 or RAMP2. We use truncation constructs, peptide competition experiments, and chimeric secretin-GLP1 receptor constructs to establish that this association is structurally specific, dependent on the intramembranous region of the RAMP and TM6 and TM7 of this receptor. There were no observed changes in secretin-stimulated cAMP, intracellular calcium, ERK1/2 phosphorylation, or receptor internalization in receptor-bearing COS or CHO-K1 cells in the presence or absence of exogenous RAMP transfection, although the secretin receptor trafficks normally to the cell surface in these cells in a RAMP-independent manner, resulting in both free and RAMP-associated receptor on the cell surface. RAMP3 association with this receptor was shown to be capable of rescuing a receptor mutant (G241C) that is normally trapped intracellularly in the biosynthetic machinery. Similarly, secretin receptor expression had functional effects on adrenomedullin activity, with increasing secretin receptor expression competing for RAMP3 association with the calcitonin receptor-like receptor to yield a functional adrenomedullin receptor. These data provide important new insights into the structural basis for RAMP3 interaction with a family B G protein-coupled receptor, potentially providing a highly selective target for drug action. This may be representative of similar interactions between other members of this receptor family and RAMP proteins

Problems associated with direct displacement-based design of concrete bridges with single-column piers, and some suggested improvements

Currently available displacement-based design (DBD) procedures for bridges are critically evaluated with a view to identifying extensions and/or modifications of the procedure, for it to be applicable to final design of a fairly broad class of bridges. An improved direct DBD procedure is presented, including a suite of comprehensive design criteria and proper consideration of the degree of fixity of the pier top. The design of an overpass bridge (originally designed to a current European Code), applying the improved ‘direct’ displacement-based design (DDBD) procedure is presented and both ‘conventional’ and displacement-based designs are assessed using non-linear response-history analysis (NLRHA); comparisons are made in terms of both economy and seismic performance of the different designs. It is seen that DDBD provided a more rational base shear distribution among piers and abutments when compared to the force-based design procedure and adequately captured the displacement pattern, closely matching the results of the more rigorous NLRHA

Structure–activity study of N-((trans)-4-(2-(7-cyano-3,4-dihydroisoquinolin-2(1H)-yl)ethyl)cyclohexyl)-1H-indole-2-carboxamide (SB269652), a bitopic ligand that acts as a negative allosteric modulator of the dopamine D2 receptor

We recently demonstrated that SB269652 (1) engages one protomer of a dopamine D2 receptor (D2R) dimer in a bitopic mode to allosterically inhibit the binding of dopamine at the other protomer. Herein, we investigate structural deter- minants for allostery, focusing on modifications to three moieties within 1. We find that orthosteric “head” groups with small 7-substituents were important to maintain the limited negative cooperativity of analogues of 1, and replacement of the tetrahydroisoquinoline head group with other D2R “privileged structures” generated orthosteric antagonists. Additionally, replacement of the cyclohexylene linker with polymethylene chains conferred linker length dependency in allosteric pharma- cology. We validated the importance of the indolic NH as a hydrogen bond donor moiety for maintaining allostery. Replacement of the indole ring with azaindole conferred a 30-fold increase in affinity while maintaining negative cooperativity. Combined, these results provide novel SAR insight for bitopic ligands that act as negative allosteric modulators of the D2R

Hybridization Assays Using an Expressible DNA Fragment Encoding Firefly Luciferase as a Label

We report the use of a new label, an expressible enzymecoding DNA fragment, for nucleic acid hybridization assays. The DNA label contains a firefly luciferase coding sequence downstream from a T7 RNA polymerase promoter. The target DNA (200 bp) is denatured and hybridized simultaneously with two oligonucleotide probes. One of the probes is immobilized in microtiter wells, via the digoxigenin/anti-digoxigenin interaction, and the other probe is biotinylated. After completion of the hybridization, the hybrids are reacted with a streptavidin-luciferase DNA complex. Subsequently, the solid-phase bound DNA is expressed by coupled transcription/ translation. The synthesized luciferase catalyzes the luminescent reaction of luciferin with O 2 and ATP. The luminescence is linearly related to the amount of target DNA in the range of 5-5000 amol. The CVs obtained for 20 and 100 amol of target are 6.5% and 10.8%, respectively (n ) 4). The specific and strong interaction between two complementary nucleic acid strands forms the basis for the development of hybridization assays. Hybridization methodology is emerging as the most promising area in laboratory medicine and has transformed the way clinical testing is realized. Previous tests have been based on the monitoring of gene products, i.e., phenotypic markers, such as oncoproteins, viral antigens, etc. In contrast, current laboratory tests that are based on hybridization allow the analysis of disease at the nucleic acid level. Thus, pre-or postnatal diagnosis of genetic disease can be accomplished by hybridization of the patient's DNA with allele-specific oligonucleotide probes that recognize mutations, deletions, or insertions causing the disease. Also, the various infectious agents can be measured in biological fluids by hybridization with specific probes. In forensic science, hybridization of DNA with minisatellite probes allows the unique identification of individuals (DNA fingerprinting). 1,2 Radioactive probes (usually labeled with 32 P), in combination with autoradiographic detection, dominated in the field of hybridization assays for more than 2 decades and provide the highest sensitivities. However, the short half-life of 32 P, the health hazards and problems associated with its use and disposal, and the long exposure times (many hours to days) required for detection have placed limitations on the routine use of hybridization assays in the clinical laboratory. The current trend in this area is toward novel nonradioactive alternatives. 2,3 The labels can be incorporated into the probes either enzymatically (e.g., using DNA polymerase or deoxynucleotidyl transferase and modified deoxynucleoside triphosphates) or by chemical conjugation (e.g., introduction of NH 2 groups into the probe via cytidine transamination and then conjugation to the reporter molecule). 4 Nonisotopic hybridization assays based on fluorescent, chemiluminescent, or enzyme labels have been developed. Generally, there are two strategies for the analysis of hybrids. Either the reporter molecule is directly conjugated to the probe, 5,6 or a ligand is attached to the probe and the hybrids are measured in a subsequent step by adding a specific, labeled binding protein. The ligand may be biotin or a hapten (e.g., digoxigenin). Labeled (strept)avidin or antihapten antibodies may then be employed for detection. 7,8 Enzymes (such as alkaline phosphatase and horseradish peroxidase) are the most widely used nonradioisotopic labels because they provide amplification through the high turnover of their substrates to detectable products. 2 Recently, we reported 9 that a DNA fragment (DNA template) coding for an enzyme can be used as a novel label for the development of highly sensitive immunoassays (expression immunoassays). In these assays, after completion of the immunoreaction, the DNA template (a luciferase-coding DNA) is expressed by in vitro transcription/translation and the activity of the synthesized enzyme is measured. Furthermore, it was estimated that 12-14 luciferase molecules were synthesized from each DNA template molecule. In the present work, we extend our investigation in the area of hybridization assays. EXPERIMENTAL SECTION Instrumentation. Luminescence measurements were carried out using a liquid scintillation counter (Model LS-6500, Beckman Instruments Inc., Fullerton, CA) in the single photon monitoring mode. Fluorescence measurements were performed with th

Advances in small lasers

M.T.H was supported by an Australian Research council Future Fellowship research grant for this work. M.C.G. is grateful to the Scottish Funding Council (via SUPA) for financial support.Small lasers have dimensions or modes sizes close to or smaller than the wavelength of emitted light. In recent years there has been significant progress towards reducing the size and improving the characteristics of these devices. This work has been led primarily by the innovative use of new materials and cavity designs. This Review summarizes some of the latest developments, particularly in metallic and plasmonic lasers, improvements in small dielectric lasers, and the emerging area of small bio-compatible or bio-derived lasers. We examine the different approaches employed to reduce size and how they result in significant differences in the final device, particularly between metal- and dielectric-cavity lasers. We also present potential applications for the various forms of small lasers, and indicate where further developments are required.PostprintPeer reviewe

Structure-Activity Analysis of Biased Agonism at the Human Adenosine A 3 Receptor s

ABSTRACT Biased agonism at G protein-coupled receptors (GPCRs) has significant implications for current drug discovery, but molecular determinants that govern ligand bias remain largely unknown. The adenosine A 3 GPCR (A 3 AR) is a potential therapeutic target for various conditions, including cancer, inflammation, and ischemia, but for which biased agonism remains largely unexplored. We now report the generation of bias "fingerprints" for prototypical ribose containing A 3 AR agonists and rigidified (N)-methanocarba 59-N-methyluronamide nucleoside derivatives with regard to their ability to mediate different signaling pathways. Relative to the reference prototypical agonist IB-MECA, (N)-methanocarba 59-Nmethyluronamide nucleoside derivatives with significant N 6 or C2 modifications, including elongated aryl-ethynyl groups, exhibited biased agonism. Significant positive correlation was observed between the C2 substituent length (in Å) and bias toward cell survival. Molecular modeling suggests that extended C2 substituents on (N)-methanocarba 59-N-methyluronamide nucleosides promote a progressive outward shift of the A 3 AR transmembrane domain 2, which may contribute to the subset of A 3 AR conformations stabilized on biased agonist binding