661 research outputs found
Two- and three-dimensional simulations of core-collapse supernovae with CHIMERA
Ascertaining the core-collapse supernova mechanism is a complex, and yet
unsolved, problem dependent on the interaction of general relativity,
hydrodynamics, neutrino transport, neutrino-matter interactions, and nuclear
equations of state and reaction kinetics. Ab initio modeling of core-collapse
supernovae and their nucleosynthetic outcomes requires care in the coupling and
approximations of the physical components. We have built our multi-physics
CHIMERA code for supernova modeling in 1-, 2-, and 3-D, using ray-by-ray
neutrino transport, approximate general relativity, and detailed neutrino and
nuclear physics. We discuss some early results from our current series of
exploding 2D simulations and our work to perform computationally tractable
simulations in 3D using the "Yin-Yang" grid.Comment: Proceedings of the 12th Symposium on Nuclei in the Cosmos. 5-12
August 2012. Cairns, Australia. Published online at
http://pos.sissa.it/archive/conferences/146/208/NIC%20XII_208.pdf Corrected
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Effects of paternal high-fat diet and maternal rearing environment on the gut microbiota and behavior.
Exposing a male rat to an obesogenic high-fat diet (HFD) influences attractiveness to potential female mates, the subsequent interaction of female mates with infant offspring, and the development of stress-related behavioral and neural responses in offspring. To examine the stomach and fecal microbiome\u27s potential roles, fecal samples from 44 offspring and stomach samples from offspring and their fathers were collected and bacterial community composition was studied by 16 small subunit ribosomal RNA (16S rRNA) gene sequencing. Paternal diet (control, high-fat), maternal housing conditions (standard or semi-naturalistic housing), and maternal care (quality of nursing and other maternal behaviors) affected the within-subjects alpha-diversity of the offspring stomach and fecal microbiomes. We provide evidence from beta-diversity analyses that paternal diet and maternal behavior induced community-wide shifts to the adult offspring gut microbiome. Additionally, we show that paternal HFD significantly altered the adult offspring Firmicutes to Bacteroidetes ratio, an indicator of obesogenic potential in the gut microbiome. Additional machine-learning analyses indicated that microbial species driving these differences converged on Bifidobacterium pseudolongum. These results suggest that differences in early-life care induced by paternal diet and maternal care significantly influence the microbiota composition of offspring through the microbiota-gut-brain axis, having implications for adult stress reactivity
Eclipse-Ballooning 2017: The U of MN – Twin Cities Experience
The stratospheric ballooning team at the U of MN – Twin Cities started working on eclipse-ballooning in the fall of 2013, even before the Montana Space Grant announced their plan to organize a national Eclipse Ballooning Project. Our team promptly signed up to assist their effort, and have been heavily involved ever since. This presentation will discuss our eclipse-ballooning efforts and progress over the past 4 years. Our experiences include experimenting with a GoPro-based video-telemetry system (which ultimately was not as successful as Montana’s Raspberry-Pi-based system), adopting (then helping test, modify, and teach other teams to learn to use) the Montana telemetry system, practicing with up-range and down-range ground station placement, developing and testing passive anti-rotation devices and active camera-pointing devices to improve video quality, landing two eclipse-telemetry systems in Minnesota lakes one week before the eclipse (ouch!), flying five balloon stacks during the eclipse from near Grand Island, NE, and organizing/hosting AHAC 2017. We will also discuss ways in which we have already begun to use the telemetry equipment for non-eclipse balloon missions. The eclipse project has greatly expanded our HAB network and ballooning capabilities in multiple different directions, and will continue to strongly influence our stratospheric ballooning program for years to come
An inclusive typology of values for navigating transformations towards a just and sustainable future
Difference-in-Differences Method in Comparative Effectiveness Research: Utility with Unbalanced Groups
Effects of atmospheric light scattering on spectroscopic observations of greenhouse gases from space. Part 2 : Algorithm intercomparison in the GOSAT data processing for CO₂ retrievals over TCCON sites
High Throughput Screening for Small Molecule Therapy for Gaucher Disease Using Patient Tissue as the Source of Mutant Glucocerebrosidase
Gaucher disease (GD), the most common lysosomal storage disorder, results from the inherited deficiency of the lysosomal enzyme glucocerebrosidase (GCase). Previously, wildtype GCase was used for high throughput screening (HTS) of large collections of compounds to identify small molecule chaperones that could be developed as new therapies for GD. However, the compounds identified from HTS usually showed reduced potency later in confirmatory cell-based assays. An alternate strategy is to perform HTS on mutant enzyme to identify different lead compounds, including those enhancing mutant enzyme activities. We developed a new screening assay using enzyme extract prepared from the spleen of a patient with Gaucher disease with genotype N370S/N370S. In tissue extracts, GCase is in a more native physiological environment, and is present with the native activator saposin C and other potential cofactors. Using this assay, we screened a library of 250,000 compounds and identified novel modulators of mutant GCase including 14 new lead inhibitors and 30 lead activators. The activities of some of the primary hits were confirmed in subsequent cell-based assays using patient-derived fibroblasts. These results suggest that primary screening assays using enzyme extracted from tissues is an alternative approach to identify high quality, physiologically relevant lead compounds for drug development
Centromere-associated topoisomerase activity in bloodstream form Trypanosoma brucei
Topoisomerase-II accumulates at centromeres during prometaphase, where it resolves the DNA catenations that represent the last link between sister chromatids. Previously, using approaches including etoposide-mediated topoisomerase-II cleavage, we mapped centromeric domains in trypanosomes, early branching eukaryotes in which chromosome segregation is poorly understood. Here, we show that in bloodstream form Trypanosoma brucei, RNAi-mediated depletion of topoisomerase-IIα, but not topoisomerase-IIβ, results in the abolition of centromere-localized activity and is lethal. Both phenotypes can be rescued by expression of the corresponding enzyme from T. cruzi. Therefore, processes which govern centromere-specific topoisomerase-II accumulation/activation have been functionally conserved within trypanosomes, despite the long evolutionary separation of these species and differences in centromeric DNA organization. The variable carboxyl terminal region of topoisomerase-II has a major role in regulating biological function. We therefore generated T. brucei lines expressing T. cruzi topoisomerase-II truncated at the carboxyl terminus and examined activity at centromeres after the RNAi-mediated depletion of the endogenous enzyme. A region necessary for nuclear localization was delineated to six residues. In other organisms, sumoylation of topoisomerase-II has been shown to be necessary for regulated chromosome segregation. Evidence that we present here suggests that sumoylation of the T. brucei enzyme is not required for centromere-specific cleavage activity
Should UI Eligibility Be Expanded to Low-Earning Workers? Evidence on Employment, Transfer Receipt, and Income from Administrative Data
Recent efforts to expand unemployment insurance (UI) eligibility are expected to increase low-earning workers’ access to UI. Although the expansion’s aim is to smooth the income and consumption of previously ineligible workers, it is possible that UI benefits simply displace other sources of income. Standard economic models predict that UI delays reemployment, thereby reducing wage income. Additionally, low-earning workers are often eligible for benefits from means-tested programs, which may decrease with UI benefits. In this paper, we estimate the impact of UI eligibility on employment, means-tested program participation, and income after job loss using a unique individual-level administrative data set from the state of Michigan. To identify a causal effect, we implement a fuzzy regression discontinuity design around the minimum earnings threshold for UI eligibility. Our main finding is that while UI eligibility increases jobless durations by up to 25 percent and temporarily lowers receipt of cash assistance (TANF) by 63 percent, the net impact on total income is still positive and large. In the quarter immediately following job loss, UI-eligible workers have 46-61 percent higher incomes than ineligibles
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