4,054 research outputs found
Integrated Regulatory and Metabolic Networks of the Marine Diatom Phaeodactylum tricornutum Predict the Response to Rising CO2 Levels.
Diatoms are eukaryotic microalgae that are responsible for up to 40% of the ocean's primary productivity. How diatoms respond to environmental perturbations such as elevated carbon concentrations in the atmosphere is currently poorly understood. We developed a transcriptional regulatory network based on various transcriptome sequencing expression libraries for different environmental responses to gain insight into the marine diatom's metabolic and regulatory interactions and provide a comprehensive framework of responses to increasing atmospheric carbon levels. This transcriptional regulatory network was integrated with a recently published genome-scale metabolic model of Phaeodactylum tricornutum to explore the connectivity of the regulatory network and shared metabolites. The integrated regulatory and metabolic model revealed highly connected modules within carbon and nitrogen metabolism. P. tricornutum's response to rising carbon levels was analyzed by using the recent genome-scale metabolic model with cross comparison to experimental manipulations of carbon dioxide. IMPORTANCE Using a systems biology approach, we studied the response of the marine diatom Phaeodactylum tricornutum to changing atmospheric carbon concentrations on an ocean-wide scale. By integrating an available genome-scale metabolic model and a newly developed transcriptional regulatory network inferred from transcriptome sequencing expression data, we demonstrate that carbon metabolism and nitrogen metabolism are strongly connected and the genes involved are coregulated in this model diatom. These tight regulatory constraints could play a major role during the adaptation of P. tricornutum to increasing carbon levels. The transcriptional regulatory network developed can be further used to study the effects of different environmental perturbations on P. tricornutum's metabolism
The DDO IVC Distance Project: Survey Description and the Distance to G139.6+47.6
We present a detailed analysis of the distance determination for one
intermediate Velocity Cloud (IVC G139.6+47.6) from the ongoing DDO IVC Distance
Project. Stars along the line of sight to G139.6+47.6 are examined for the
presence of sodium absorption attributable to the cloud, and the distance
bracket is established by astrometric and spectroscopic parallax measurements
of demonstrated foreground and background stars. We detail our strategy
regarding target selection, observational setup, and analysis of the data,
including a discussion of wavelength calibration and sky subtraction
uncertainties. We find a distance estimate of 129 (+/- 10) pc for the lower
limit and 257 (+211-33) pc for the upper limit. Given the high number of stars
showing absorption due to this IVC, we also discuss the small-scale covering
factor of the cloud and the likely significance of non-detections for
subsequent observations of this and other similar IVC's. Distance measurements
of the remaining targets in the DDO IVC project will be detailed in a companion
paper.Comment: 10 pages, 6 figures, LaTe
Genomes of uncultured eukaryotes: sorting FACS from fiction
A recent study explores the genome content of uncultured unicellular marine eukaryotes and provides insights about interactions between uncultured eukaryotes and other biological entities
C3 Photosynthesis in the Gametophyte of the Epiphytic CAM Fern Pyrrosia Longifolia (Polypodiaceae)
This is the publisher's official version, also available at: http://www.jstor.org/stable/2445689Sporophytes of some epiphytic species in the fern genus Pyrrosia exhibit Crassulacean acid metabolism (CAM), generally
considered to be a derived physiological response to xeric habitats. Because these species alternate between independent
sporophytic and gametophytic generations yet only the sporophyte has been characterized physiologically, experiments were
conducted to determine the photosynthetic pathways present in mature sporophytes, immature sporophytes, and gametophytes
of Pyrrosia longifolia. Diurnal C 0 2 exchange and malic acid fluctuations demonstrated that although the mature
sporophytes exhibited CAM, only C3 photosynthesis occurred in the gametophytes and young sporophytes. Consideration
of the above results and those from previous studies, as well as the life cycle of ferns, indicates that the induction of CAM
probably occurs at a certain developmental stage of the sporophyte and/or following exposure to stress. Elucidation of the
precise mechanisms underlying this C^-CAM transition awaits further research
The series Bi2Sr2Ca(n-1) Cu(n)O(2n+4) (1 less than or equal to n less than or equal to 5): Phase stability and superconducting properties
Phase relations at 850 and 870 C, melting transitions in air, oxygen, and helium were studied for Bi(2.1)Sr(1.9) CuO6 and for the Bi2Sr2Ca(n-1) Cu(n)O(2n+4) for n = 1, 2, 3, 4, 5, and infinity (CaCuO2). Up to 870 C, the n = 2 composition resides in the compatibility tetrahedron bounded by Bi(2+x)(Sr,Ca)(3-y) Cu2O8, (Sr,Ca)14 Cu24O41, Ca2CuO3, and a Bi-Sr-Ca-O phase. The n is greater than or equal to 3 compositions reside in the compatibility tetrahedron Bi(2+x)(Sr,Ca)(3-y) Cu2O8 - (Sr,Ca)14 Cu24O41 - Ca2CuO3 - CuO up to 850 C. However, Bi(2+x)Sr(4-y) Cu3O10 forms for n is greater than or equal to 3 after extended heating at 870 C. Bi(2+x)Sr(2-y) CuO6 and Bi(2+x)(Sr,Ca)(3-y) Cu2O8 melt in air at 914 C and 895 C respectively. During melting, all of the compositions studied lose 1 to 2 percent by weight of oxygen from the reduction of copper. Bi(2+x)Sr(2-y) CuO6, Bi(2+n)(Sr,Ca)(3-y) Cu2O8, and Bi(2+x)(Sr,Ca)(4-y) Cu3O10 exhibit crystallographic alignment in a magnetic field, with the c-axes orienting parallel to the field
Scaling Up Psychological Treatments: A Countrywide Test of the Online Training of Therapists.
BACKGROUND: A major barrier to the widespread dissemination of psychological treatments is the way that therapists are trained. The current method is not scalable. OBJECTIVE: Our objective was to conduct a proof-of-concept study of Web-centered training, a scalable online method for training therapists. METHODS: The Irish Health Service Executive identified mental health professionals across the country whom it wanted to be trained in a specific psychological treatment for eating disorders. These therapists were given access to a Web-centered training program in transdiagnostic cognitive behavior therapy for eating disorders. The training was accompanied by a scalable form of support consisting of brief encouraging telephone calls from a nonspecialist. The trainee therapists completed a validated measure of therapist competence before and after the training. RESULTS: Of 102 therapists who embarked upon the training program, 86 (84.3%) completed it. There was a substantial increase in their competence scores following the training (mean difference 5.84, 95% Cl -6.62 to -5.05; P<.001) with 42.5% (34/80) scoring above a predetermined cut-point indicative of a good level of competence. CONCLUSIONS: Web-centered training proved feasible and acceptable and resulted in a marked increase in therapist competence scores. If these findings are replicated, Web-centered training would provide a means of simultaneously training large numbers of geographically dispersed trainees at low cost, thereby overcoming a major obstacle to the widespread dissemination of psychological treatments
Transcriptomes of parents identify parenting strategies and sexual conflict in a subsocial beetle
This work was funded by UK NERC grants to M.G.R. and A.J.M. an NERC studentship to D.J.P. the University of Georgia and a US NSF grant to A.J.M. and M.G.R.Parenting in the burying beetle Nicrophorus vespilloides is complex and, unusually, the sex and number of parents that can be present is flexible. Such flexibility is expected to involve specialized behaviour by the two sexes under biparental conditions. Here, we show that offspring fare equally well regardless of the sex or number of parents present. Comparing transcriptomes, we find a largely overlapping set of differentially expressed genes in both uniparental and biparental females and in uniparental males including vitellogenin, associated with reproduction, and takeout, influencing sex-specific mating and feeding behaviour. Gene expression in biparental males is similar to that in non-caring states. Thus, being ‘biparental’ in N. vespilloides describes the family social organization rather than the number of directly parenting individuals. There was no specialization; instead, in biparental families, direct male parental care appears to be limited with female behaviour unchanged. This should lead to strong sexual conflict.Publisher PDFPeer reviewe
Liveness-Driven Random Program Generation
Randomly generated programs are popular for testing compilers and program
analysis tools, with hundreds of bugs in real-world C compilers found by random
testing. However, existing random program generators may generate large amounts
of dead code (computations whose result is never used). This leaves relatively
little code to exercise a target compiler's more complex optimizations.
To address this shortcoming, we introduce liveness-driven random program
generation. In this approach the random program is constructed bottom-up,
guided by a simultaneous structural data-flow analysis to ensure that the
generator never generates dead code.
The algorithm is implemented as a plugin for the Frama-C framework. We
evaluate it in comparison to Csmith, the standard random C program generator.
Our tool generates programs that compile to more machine code with a more
complex instruction mix.Comment: Pre-proceedings paper presented at the 27th International Symposium
on Logic-Based Program Synthesis and Transformation (LOPSTR 2017), Namur,
Belgium, 10-12 October 2017 (arXiv:1708.07854
HST/STIS Spectroscopy of the Lyman-Alpha Emission Line in the Central Dominant Galaxies in A426, A1795, and A2597: Constraints on Clouds in the Intracluster Medium
We report on HST/STIS spectra of the Lyman-alpha emission in the central
dominant galaxies in three rich clusters of galaxies. We find evidence for a
population of clouds in the intracluster medium.We detect 10 Ly-alpha
absorption systems towards the nucleus of NGC1275 with columns of N(HI)
1E12-1E14 cm-2. The detected absorption features are most consistent with
associated nuclear absorption systems. There is very little nuclear absorption
at the systemic velocity in NGC1275. This implies that the large columns
detected in the 21 cm line towards the parsec scale radio source avoid the line
of sight to the nucleus. This gas may be located in a circumnuclear disk or
torus. We detect at least one and possibly two absorption features towards the
extended Ly-alpha in A426. We do not detect absorption towards the extended
Ly-alpha emission in A1795, and A2597 with upper limits N(HI) 1E13 cm-2 for
optically thin absorbers. Our data constrain the covering factor of any high
column density gas in the ICM to be less than 25%. Our results suggest that the
lack of observed intermediate temperature gas is not explained by obscuration.
In addition, the low columns of gas on the 100 kpc scales in the ICM suggests
that (1) the rate at which cold gas accumulates in the ICM on these scales is
very low, and (2) the dense nebulae in the central 10 kpc must have cooled or
been deposited in situ.Comment: 6 figure
Spitzer observations of the Massive star forming complex S254-S258: structure and evolution
We present Spitzer-IRAC, NOAO 2.1meter-Flamingos, Keck-NIRC, and
FCRAO-SEQUOIA observations of the massive star forming complex S254-S258,
covering an area of 25x20 arc-minutes. Using a combination of the IRAC and NIR
data, we identify and classify the young stellar objects (YSO) in the complex.
We detect 510 sources with near or mid IR-excess, and we classify 87 Class I,
and 165 Class II sources. The YSO are found in clusters surrounded by isolated
YSO in a low-density distributed population. The ratio of clustered to total
YSO is 0.8. We identify six new clusters in the complex. One of them,
G192.63-00, is located around the ionizing star of the HII region S255. We
hypothesize that the ionizing star of S255 was formed in this cluster. We also
detect a southern component of the cluster in HII region S256. The cluster
G192.54-0.15, located inside HII region S254 has a VLSR of 17 km/s with respect
to the main cloud, and we conclude that it is located in the background of the
complex. The structure of the molecular cloud is examined using 12CO and 13CO,
as well as a near-IR extinction map. The main body of the molecular cloud has
VLSR between 5 and 9 km/s. The arc-shaped structure of the molecular cloud,
following the border of the HII regions, and the high column density in the
border of the HII regions support the idea that the material has been swept up
by the expansion of the HII regions.Comment: Accepted for publication in The Astrophysical Journa
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