I wish he'd listen: Client-centered interviewing approaches are associated with higher compliance with behavioral modification advice in pet dog owners

In the UK, over 40,000 dogs are given up annually to shelters or euthanized due to problem behaviors. It may be possible to reduce these numbers through behavior counseling and development of a behavior modification plan (BMP) by a canine professional (CP). However, if the client does not or cannot adhere to the BMP the dog's prospects may be compromised. This study explored the experience of the initial behavior consultation and possible reasons for adhering to (or not) the BMP from the client's perspective. An online survey solicited the opinions of canine behavior clients who had sought professional help in the UK for their dog's unwanted behavior within the last 2 years. Principal Component Analysis of Likert scale statements revealed one significant PC (P < 0.001) that explained 57% of the variation in the data and was significantly correlated with BMP compliance (r = 0.567, P < 0.001). Specifically, believing the plan was right for their dog and having CP support throughout to achieve behavior improvement through the implementation of a mutually agreed BMP were important. Qualitative thematic analysis of free text responses regarding motivation for future client BMP compliance echoed these factors. Conversely, a negative consultation experience was created by CPs adopting an authoritarian or ‘telling’ approach with their clients for example, making them feel judged. This was associated with a lack of BMP compliance. Essentially, CPs who involved their clients in BMP development were perceived as creating a positive experience of the initial behavior consultation and as a result were able to promote client BMP adherence and improvement in unwanted behavior improvement. This CP approach, which adopts a nurturing rather than an authoritarian strategy, has been termed Client-Centered Interviewing (CCI). The main thing about CCI is the client is an equal partner in the process. The core conditions are as per Rogers and Egan of empathy, congruence and unconditional positive regard. CCI builds on empathy with the client, avoids inappropriately challenging client beliefs by gently exploring options without being judgemental, clearly explains the likely cause of the behavior and the plan to resolve it, and provides a BMP that is bespoke and flexible. Future research is required to validate the findings, for example through a prospective comparison of Client-Centered Interviewing versus an instructional (authoritarian) approach. Crucially, the impact of Client-Centered Interviewing on canine welfare must also be evaluated

A survey of dog behavior modification practice in the UK: Who is offering it, what methods are they using and how effective do their clients perceive practitioners to be?

Thousands of dogs are relinquished each year in the UK owing to behavioral problems. Hence, there is a clear role for behavior modification therapy in the mitigation of this canine welfare risk. Since statutory regulation and a universal register of canine professionals (CPs) does not yet exist in the UK, it remains unclear who is offering such therapy, which behaviors are being treated, what types of approaches various CPs may be taking and the success (or otherwise) CPs are having. This study aimed to provide some insight into these issues from the perspective of pet dog owners (clients). An online survey of 235 participants showed that the primary reason for seeking behavior help from a CP was related to aggression, although often more than one issue was reported. Regardless of the behavior problem, no significant differences were found for the type of CP consulted (dog trainer versus behaviorist). Furthermore, in the client's opinion, there were no significant differences between CP types in their ability to improve their dog's unwanted behavior. Interestingly, behaviorists were significantly more likely than dog trainers to use ‘reward-based’ methods over ‘balanced training’ (balanced being a mix of reward and punishment; =8.226, df=1, p=0.004). In conclusion, in the current UK vacuum of statutory regulation, clients are just as likely to employ a trainer as a behaviorist, regardless of their dog's unwanted behavior. However, both CP types were equally able to facilitate behavior improvement, as reported by the client, regardless of the type of training methods (reward-based versus balanced) adopted. This study raises further questions to be explored including the client's opinion of the extent to which the ends (behavior outcomes) justify the means (e.g. punitive training methods which are likely to be aversive for the dog). Also, the general public's understanding of the potential welfare implications of the type of behavior modification plan they may or may not choose to follow

Predicting the public health benefit of vaccinating cattle against Escherichia coli O157

Identifying the major sources of risk in disease transmission is key to designing effective controls. However, understanding of transmission dynamics across species boundaries is typically poor, making the design and evaluation of controls particularly challenging for zoonotic pathogens. One such global pathogen is Escherichia coli O157, which causes a serious and sometimes fatal gastrointestinal illness. Cattle are the main reservoir for E. coli O157, and vaccines for cattle now exist. However, adoption of vaccines is being delayed by conflicting responsibilities of veterinary and public health agencies, economic drivers, and because clinical trials cannot easily test interventions across species boundaries, lack of information on the public health benefits. Here, we examine transmission risk across the cattle–human species boundary and show three key results. First, supershedding of the pathogen by cattle is associated with the genetic marker stx2. Second, by quantifying the link between shedding density in cattle and human risk, we show that only the relatively rare supershedding events contribute significantly to human risk. Third, we show that this finding has profound consequences for the public health benefits of the cattle vaccine. A naïve evaluation based on efficacy in cattle would suggest a 50% reduction in risk; however, because the vaccine targets the major source of human risk, we predict a reduction in human cases of nearly 85%. By accounting for nonlinearities in transmission across the human–animal interface, we show that adoption of these vaccines by the livestock industry could prevent substantial numbers of human E. coli O157 cases

Applying phylogenomics to understand the emergence of Shiga Toxin producing Escherichia coli O157:H7 strains causing severe human disease in the United Kingdom

Shiga Toxin producing Escherichia coli (STEC) O157:H7 is a recently emerged zoonotic pathogen with considerable morbidity. Since the serotype emerged in the 1980s, research has focussed on unravelling the evolutionary events from the E. coli O55:H7 ancestor to the contemporaneous globally dispersed strains. In this study the genomes of over 1000 isolates from human clinical cases and cattle, spanning the history of STEC O157:H7 in the United Kingdom were sequenced. Phylogenetic analysis reveals the ancestry, key acquisition events and global context of the strains. Dated phylogenies estimate the time to the most recent common ancestor of the current circulating global clone to 175 years ago, followed by rapid diversification. We show the acquisition of specific virulence determinates occurred relatively recently and coincides with its recent detection in the human population. Using clinical outcome data from 493 cases of STEC O157:H7 we assess the relative risk of severe disease including HUS from each of the defined clades in the population and show the dramatic effect Shiga toxin complement has on virulence. We describe two strain replacement events that have occurred in the cattle population in the UK over the last 30 years; one resulting in a highly virulent strain that has accounted for the majority of clinical cases in the UK over the last decade. This work highlights the need to understand the selection pressures maintaining Shiga-toxin encoding bacteriophages in the ruminant reservoir and the study affirms the requirement for close surveillance of this pathogen in both ruminant and human populations

Human viruses:discovery and emergence

There are 219 virus species that are known to be able to infect humans. The first of these to be discovered was yellow fever virus in 1901, and three to four new species are still being found every year. Extrapolation of the discovery curve suggests that there is still a substantial pool of undiscovered human virus species, although an apparent slow-down in the rate of discovery of species from different families may indicate bounds to the potential range of diversity. More than two-thirds of human viruses can also infect non-human hosts, mainly mammals, and sometimes birds. Many specialist human viruses also have mammalian or avian origins. Indeed, a substantial proportion of mammalian viruses may be capable of crossing the species barrier into humans, although only around half of these are capable of being transmitted by humans and around half again of transmitting well enough to cause major outbreaks. A few possible predictors of species jumps can be identified, including the use of phylogenetically conserved cell receptors. It seems almost inevitable that new human viruses will continue to emerge, mainly from other mammals and birds, for the foreseeable future. For this reason, an effective global surveillance system for novel viruses is needed

Vulnerability of the British swine industry to Classical Swine Fever

Classical swine fever (CSF) is a notifiable, highly contagious viral disease of swine which results in severe welfare and economic consequences (e.g. barrier to trade) in affected countries. In order to improve preparedness for disease incursion, it is critical to have some understanding of how vulnerable a CSF-free swine industry may be and, thus, evaluate how CSF would spread should it be introduced

How predation and landscape fragmentation affect vole population dynamics

Background: Microtine species in Fennoscandia display a distinct north-south gradient from regular cycles to stable populations. The gradient has often been attributed to changes in the interactions between microtines and their predators. Although the spatial structure of the environment is known to influence predator-prey dynamics of a wide range of species, it has scarcely been considered in relation to the Fennoscandian gradient. Furthermore, the length of microtine breeding season also displays a north-south gradient. However, little consideration has been given to its role in shaping or generating population cycles. Because these factors covary along the gradient it is difficult to distinguish their effects experimentally in the field. The distinction is here attempted using realistic agent-based modelling. Methodology/Principal Findings: By using a spatially explicit computer simulation model based on behavioural and ecological data from the field vole (Microtus agrestis), we generated a number of repeated time series of vole densities whose mean population size and amplitude were measured. Subsequently, these time series were subjected to statistical autoregressive modelling, to investigate the effects on vole population dynamics of making predators more specialised, of altering the breeding season, and increasing the level of habitat fragmentation. We found that fragmentation as well as the presence of specialist predators are necessary for the occurrence of population cycles. Habitat fragmentation and predator assembly jointly determined cycle length and amplitude. Length of vole breeding season had little impact on the oscillations. Significance: There is good agreement between our results and the experimental work from Fennoscandia, but our results allow distinction of causation that is hard to unravel in field experiments. We hope our results will help understand the reasons for cycle gradients observed in other areas. Our results clearly demonstrate the importance of landscape fragmentation for population cycling and we recommend that the degree of fragmentation be more fully considered in future analyses of vole dynamics

Understanding foot-and-mouth disease virus transmission biology: identification of the indicators of infectiousness

The control of foot-and-mouth disease virus (FMDV) outbreaks in non-endemic countries relies on the rapid detection and removal of infected animals. In this paper we use the observed relationship between the onset of clinical signs and direct contact transmission of FMDV to identify predictors for the onset of clinical signs and identify possible approaches to preclinical screening in the field. Threshold levels for various virological and immunological variables were determined using Receiver Operating Characteristic (ROC) curve analysis and then tested using generalized linear mixed models to determine their ability to predict the onset of clinical signs. In addition, concordance statistics between qualitative real time PCR test results and virus isolation results were evaluated. For the majority of animals (71%), the onset of clinical signs occurred 3–4 days post infection. The onset of clinical signs was associated with high levels of virus in the blood, oropharyngeal fluid and nasal fluid. Virus is first detectable in the oropharyngeal fluid, but detection of virus in the blood and nasal fluid may also be good candidates for preclinical indicators. Detection of virus in the air was also significantly associated with transmission. This study is the first to identify statistically significant indicators of infectiousness for FMDV at defined time periods during disease progression in a natural host species. Identifying factors associated with infectiousness will advance our understanding of transmission mechanisms and refine intra-herd and inter-herd disease transmission models

Epidemiology of antimicrobial-resistant Escherichia coli carriage in sympatric humans and livestock in a rapidly urbanizing city

There are substantial limitations in understanding of the distribution of antimicrobial resistance (AMR) in humans and livestock in developing countries. This papers present the results of an epidemiological study examining patterns of AMR in Escherichia coli isolates circulating in sympatric human (n = 321) and livestock (n = 633) samples from 99 households across Nairobi, Kenya. E. coli isolates were tested for susceptibility to 13 antimicrobial drugs representing nine antibiotic classes. High rates of AMR were detected, with 47.6% and 21.1% of isolates displaying resistance to three or more and five or more antibiotic classes, respectively. Human isolates showed higher levels of resistance to sulfonamides, trimethoprim, aminoglycosides and penicillins compared with livestock (P0.05). These findings revealed a high prevalence of AMR E. coli circulating in healthy humans and livestock in Nairobi, with no evidence to suggest that keeping livestock, when treated as a single risk factor, contributed significantly to the burden of AMR in humans, although the presence of livestock waste was significant. These results provide an understanding of the broader epidemiology of AMR in complex and interconnected urban environments

Heterogeneities in leishmania infantum infection : using skin parasite burdens to identify highly infectious dogs

Background: The relationships between heterogeneities in host infection and infectiousness (transmission to arthropod vectors) can provide important insights for disease management. Here, we quantify heterogeneities in Leishmania infantum parasite numbers in reservoir and non-reservoir host populations, and relate this to their infectiousness during natural infection. Tissue parasite number was evaluated as a potential surrogate marker of host transmission potential. Methods: Parasite numbers were measured by qPCR in bone marrow and ear skin biopsies of 82 dogs and 34 crab-eating foxes collected during a longitudinal study in Amazon Brazil, for which previous data was available on infectiousness (by xenodiagnosis) and severity of infection. Results: Parasite numbers were highly aggregated both between samples and between individuals. In dogs, total parasite abundance and relative numbers in ear skin compared to bone marrow increased with the duration and severity of infection. Infectiousness to the sandfly vector was associated with high parasite numbers; parasite number in skin was the best predictor of being infectious. Crab-eating foxes, which typically present asymptomatic infection and are non-infectious, had parasite numbers comparable to those of non-infectious dogs. Conclusions: Skin parasite number provides an indirect marker of infectiousness, and could allow targeted control particularly of highly infectious dogs