Primer reporte de secuencia de ITS de Ustilago Quitensis en Cortaderia jubata en Ecuador

Cortaderia jubata es un pasto común a lo largo de las carreteras en las regiones andinas de Ecuador. Ocasionalmente, algunas de estas plantas se enferman. Uno de los hongos que causan estas enfermedades, Ustilago quitensis, es un carbón que infecta a las inflorescencias de la planta. Ustilago quitensis (syn. U. cortaderiae) infecta a C. jubata y otras especies de Cortaderia; se piensa que está distribuido en toda América Central y del Sur, pero solo ha sido reportado en Argentina, Bolivia, Chile y Ecuador. Ustilago quitensis ha sido reportado específicamente infectando C. jubata en Bolivia y Ecuador. En este reporte se documenta por primera vez la secuencia de ADN de la región de espaciadores internos que no se transcriben (ITS) de U. quitensis en C. jubata en Ecuador. Se describe, además, la morfología de las teliosporas. La secuencia de ADN puede utilizarse para entender la relación filogenética del carbón que infecta varias especies de Cortaderia en América Central y del Sur.Cortaderia jubata is a common grass along the roadsides of the Andean regions of Ecuador. Occasionally, some of these plants are infected by disease. One of these diseases, Ustilago quitensis, is a smut fungus that infects the seed heads or panicle of the plant. Ustilago quitensis (syn. U. cortaderiae) infecting C. jubata and other Cortaderia species is thought to occur throughout Central and South America, but has only been reported in Argentina, Bolivia, Chile, and Ecuador. Ustilago quitensis has only been reported specifically infecting C. jubata in Bolivia and Ecuador. In this report we document for the first time the DNA sequence of the internal transcribed spacer region (ITS) of U. quitensis on C. jubata in Ecuador. Additionally, we describe the morphology of the teliospores. The DNA sequence could be used to understand the phylogenetic relationship of the smut fungus infecting various Cortaderia species in Central and South America.Fil: Barnes, Charlie Wesley. Universidad Tecnológica Indoamérica. Centro de Investigación de la Biodiversidad y el Cambio Climático; EcuadorFil: Ordóñez Maldonado, Maria Eugenia. Pontificia Universidad Católica del Ecuador; EcuadorFil: Testoni, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Villamil, Carlos Baldomero. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentin

Human SNP links differential outcomes in inflammatory and infectious disease to a FOXO3-regulated pathway

The clinical course and eventual outcome, or prognosis, of complex diseases varies enormously between affected individuals. This variability critically determines the impact a disease has on a patient’s life but is very poorly understood. Here, we exploit existing genome-wide association study data to gain insight into the role of genetics in prognosis. We identify a noncoding polymorphism in FOXO3A (rs12212067: T > G) at which the minor (G) allele, despite not being associated with disease susceptibility, is associated with a milder course of Crohn’s disease and rheumatoid arthritis and with increased risk of severe malaria. Minor allele carriage is shown to limit inflammatory responses in monocytes via a FOXO3-driven pathway, which through TGFβ1 reduces production of proinflammatory cytokines, including TNFα, and increases production of anti-inflammatory cytokines, including IL-10. Thus, we uncover a shared genetic contribution to prognosis in distinct diseases that operates via a FOXO3-driven pathway modulating inflammatory responses. PAPERCLIP

Development and regeneration of the crushing dentition of skates (Rajidae)

Sharks and rays (elasmobranchs) have the remarkable capacity to continuously regenerate their teeth. The polyphyodont system is considered the ancestral condition of the gnathostome dentition. Despite this shared regenerative ability, sharks and rays exhibit dramatic interspecific variation in their tooth morphology. Ray (batoidea) teeth typically constitute crushing pads of flattened teeth, whereas shark teeth are pointed, multi-cuspid units. Although recent research has addressed the molecular development of the shark dentition, little is known about that of the ray. Furthermore, how dental diversity within the elasmobranch lineage is achieved remains unknown. Here, we examine dental development and regeneration in two Batoid species: the thornback skate (Raja clavata) and the little skate (Leucoraja erinacea). Using in situ hybridization and immunohistochemistry, we examine the expression of a core gnathostome dental gene set during early development of the skate dentition and compare it to development in the shark. Elasmobranch tooth development is highly conserved, with sox2 likely playing an important role in the initiation and regeneration of teeth. Alterations to conserved genes expressed in an enamel knot-like signalling centre may explain the morphological diversity of elasmobranch teeth, thereby enabling sharks and rays to occupy diverse dietary and ecological niches

Thiopurine monotherapy is effective in ulcerative colitis but significantly less so in Crohn’s disease: long-term outcomes for 11 928 patients in the UK inflammatory bowel disease bioresource

Objective: Thiopurines are widely used as maintenance therapy in inflammatory bowel disease (IBD) but the evidence base for their use is sparse and their role increasingly questioned. Using the largest series reported to date, we assessed the long-term effectiveness of thiopurines in ulcerative colitis (UC) and Crohn’s disease (CD), including their impact on need for surgery. Design: Outcomes were assessed in 11 928 patients (4968 UC, 6960 CD) in the UK IBD BioResource initiated on thiopurine monotherapy with the intention of maintaining medically induced remission. Effectiveness was assessed retrospectively using patient-level data and a definition that required avoidance of escalation to biological therapy or surgery while on thiopurines. Analyses included overall effectiveness, time-to-event analysis for treatment escalation and comparison of surgery rates in patients tolerant or intolerant of thiopurines. Results: Using 68 132 patient-years of exposure, thiopurine monotherapy appeared effective for the duration of treatment in 2617/4968 (52.7%) patients with UC compared with 2378/6960 (34.2%) patients with CD (p<0.0001). This difference was corroborated in a multivariable analysis: after adjusting for variables including treatment era, thiopurine monotherapy was less effective in CD than UC (OR 0.47, 95% CI 0.43 to 0.51, p<0.0001). Thiopurine intolerance was associated with increased risk of surgery in UC (HR 2.44, p<0.0001); with a more modest impact on need for surgery in CD (HR=1.23, p=0.0015). Conclusion: Thiopurine monotherapy is an effective long-term treatment for UC but significantly less effective in CD

HLA-DQA1*05 carriage associated with development of anti-drug antibodies to infliximab and adalimumab in patients with Crohn's Disease

Anti-tumor necrosis factor (anti-TNF) therapies are the most widely used biologic drugs for treating immune-mediated diseases, but repeated administration can induce the formation of anti-drug antibodies. The ability to identify patients at increased risk for development of anti-drug antibodies would facilitate selection of therapy and use of preventative strategies.This article is freely available via Open Access. Click on Publisher URL to access the full-text

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Salman Rushdie\u27s Authorial Self-Fashioning in Joseph Anton

This article examines some of the highlights, limitations, and contradictions of Rushdie’s authorial personas that have been perpetuated and challenged by his critics and the mass media. I argue that Joseph Anton, published in 2012, exhibits evidence of Rushdie’s attempt at authorial self-fashioning, and therefore the memoir represents an important part of his effort to shape the public narrative about him. Joseph Anton highlights Rushdie’s exilic persona through direct comparisons to figures like Voltaire and Galileo, and attempts to privilege this position above his other authorial selves. This authorial self has deep roots in a narrative fashioned by Rushdie that has been abetted by some of his critics and the media since the fatwa. My essay critiques this emphasis, suggesting that Rushdie’s self-fashioning is out of step with his twenty-first-century political ideals and affiliations. Ultimately, the third-person “distancing” of the memoir helps to highlight what it seeks to mitigate: a plurality of Rushdie’s competing for public attention

Inscriptions of resistance in Joseph Conrad\u27s Heart of Darkness

The possibility of native resistance to colonial tyranny and the threat of the loss of colonial order is a continual, sustained anxiety throughout Joseph Conrad\u27s novella Heart of Darkness. Critics have largely ignored or downplayed these inscriptions of resistance in Conrad\u27s text. Much of the criticism that surrounds this novella, according to Patrick Brantlinger, is focused on the European subjects of the text, and therefore renders Africa and its native peoples as a kind of backdrop. Literary critiques of Heart of Darkness that do discuss the African natives tend to portray them as victims rather than having any kind of agency. This latent fear of native resistance demonstrates the fantasy of stability and superiority endemic to imperialism: a narrative that the imperial administration must continually tell itself