Risk Factors for Online and In-person Stalking, Intrusive Harassment, and Violence within a Community Sample

Stalking and intrusive harassment (SIH) and stalking violence are prevalent and serious public health concerns associated with significant physical, psychological, financial, and social consequences. The growing proliferation of technology and the internet has only exacerbated the problem as SIH behaviors can be easily perpetrated via electronic means. Given its prevalence and negative impact, it is imperative to investigate the risk factors for online and in-person SIH and stalking violence to better predict and mitigate its detrimental effects. The current study therefore aimed to 1) examine the descriptive characteristics associated with online and in-person SIH and stalking violence; and 2) identify shared and differential risk factors for online and in-person SIH, as well as minor (e.g., slapping, pushing, throwing an object) and severe (e.g., kicking, choking, burning, using a weapon, forcing into sexual activities) stalking violence, in a community sample. Towards these aims, 561 participants completed an online survey comprised of a battery of self-report measures that assessed various predisposing (i.e., demographic, historical, psychological, dispositional, cognitive, and affective) and contextual factors (e.g., substance use, negative affect) and engagement in SIH and stalking violence. A little less than half of the sample reported perpetrating SIH, with most engaging in both online and in-person SIH (i.e., mixed SIH). Approximately one-third of these harassers/stalkers endorsed engaging in some form of stalking violence. Overall, various predisposing and contextual risk factors were associated with SIH and stalking violence, with those exhibiting more extreme or elevated levels of the relevant risk factors being more likely to engage in mixed SIH and potentially more serious stalking violence (e.g., choking, using a weapon). Notably, the findings of this study demonstrated the critical importance of considering both predisposing and contextual risk factors together to enhance risk assessment and management efforts. Additionally, the current study emphasized the importance of assessing for online SIH, especially when perpetrated in conjunction with in-person SIH, as it was associated with a heightened risk for potentially more serious stalking violence. This is crucial given the continued proliferation and advancement of technology and electronic communication. The findings from this study present significant implications for the development and implementation of relevant law, policy, and evidence-based risk assessment and intervention strategies. Advisor: Mario J. Scalor

kb_DRAM: annotation and metabolic profiling of genomes with DRAM in KBase

Microbial genome annotation is the process of identifying structural and functional elements in DNA sequences and subsequently attaching biological information to those elements. DRAM is a tool developed to annotate bacterial, archaeal, and viral genomes derived from pure cultures or metagenomes. DRAM goes beyond traditional annotation tools by distilling multiple gene annotations to genome level summaries of functional potential. Despite these benefits, a downside of DRAM is the requirement of large computational resources, which limits its accessibility. Further, it did not integrate with downstream metabolic modeling tools that require genome annotation. To alleviate these constraints, DRAM and the viral counterpart, DRAM-v, are now available and integrated with the freely accessible KBase cyberinfrastructure. With kb_DRAM users can generate DRAM annotations and functional summaries from microbial or viral genomes in a point-and-click interface, as well as generate genome-scale metabolic models from DRAM annotations.Availability and implementationFor kb_DRAM users, the kb_DRAM apps on KBase can be found in the catalog at https://narrative.kbase.us/#catalog/modules/kb_DRAM. For kb_DRAM users, a tutorial workflow with all documentation is available at https://narrative.kbase.us/narrative/129480. For kb_DRAM developers, software is available at https://github.com/shafferm/kb_DRAM

A Future for the Dead Sea Basin: Water Culture among Israelis, Palestinians and Jordanians

The Dead Sea basin plays a major role for regional economic development (industry, tourism and agriculture) in the Middle East. This potential is threatened by the steady disappearance of the Dead Sea. Since around 1930 the water level of the Dead Sea has fallen by about 25 m, about half of this alone in the last 20 years. The Dead Sea is a transboundary resource shared by Israel, the Palestinian Authority and Jordan. The Dead Sea is the terminal point of the Jordan River watershed and as such, it serves as a barometer for the health of the overall system. Its rapid decline reflects the present water management strategies of the riparian and upstream countries. This includes the different water cultures of the three countries. Throughout history, the Dead Sea basin has served as a source of refuge and inspiration for followers of Judaism, Christianity and Islam. Today, the religious significance of the Dead Sea is being overshadowed by its rapid disappearance. This may be explained in part by the water cultures of the three countries that influence water policy in the region. Ideology, together with culture and tradition, such as that of Zionism in Israel, has played a central role in water development in the region. In many cases, this has been at the expense of the environment. Elements pertaining to environmental security and water culture and tradition, whereby a sustainably managed environment provides for social, economic as well as environmental benefits are evident with regards the Dead Sea. The decline for example, undermines its potential as a tourist destination, despite the enormous investment in hotel and resort infrastructures in Israel and in Jordan. The decline also raises ethical issues about the exploitation of water resources by present generations at the expense of this natural heritage to future generations. This paper provides an analysis of a European Union funded project whose aims are to synthesize and assess existing physical and socio-economic data and to assess options for a better future for the Dead Sea. It will identify the patterns of water supply and use in the region, and the factors that control these patterns, including those of water culture. The underlying assumption is that solutions for a more sustainable development than todays scenario will not come from simply providing more water for more development, but from a new land and water management system, indeed ethic, that is sensitive to social, cultural and ecological resources thereby providing security and stability across cultures, economic sectors and nations

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Data Descriptor: An open resource for transdiagnostic research in pediatric mental health and learning disorders

Technological and methodological innovations are equipping researchers with unprecedented capabilities for detecting and characterizing pathologic processes in the developing human brain. As a result, ambitions to achieve clinically useful tools to assist in the diagnosis and management of mental health and learning disorders are gaining momentum. To this end, it is critical to accrue large-scale multimodal datasets that capture a broad range of commonly encountered clinical psychopathology. The Child Mind Institute has launched the Healthy Brain Network (HBN), an ongoing initiative focused on creating and sharing a biobank of data from 10,000 New York area participants (ages 5–21). The HBN Biobank houses data about psychiatric, behavioral, cognitive, and lifestyle phenotypes, as well as multimodal brain imaging (resting and naturalistic viewing fMRI, diffusion MRI, morphometric MRI), electroencephalography, eyetracking, voice and video recordings, genetics and actigraphy. Here, we present the rationale, design and implementation of HBN protocols. We describe the first data release (n =664) and the potential of the biobank to advance related areas (e.g., biophysical modeling, voice analysis

Severity of Nausea and Vomiting in Pregnancy and Early Childhood Neurobehavioural Outcomes

Developmental Origins of Health and Disease (DOHaD) International Society meeting35198-10