BA in Photographic Media Graduate Show 2015

The work seen in this book relates to the students’ major final photographic project of 20 Credits, consisting of: Book/Folio, Written Visual Diary to support the project + Exhibition. Anthony Griffin Camila Brandao Carole McGloughlin Caroline Brady Cristina Llarena Devi Anna Chacon Cones Diego Ehlers Fabro Emma Nilssens-Hanseen Gudrun Borgan Holmen Kaja Eline Sitron Juul Markus Rollbuhler Marta Czajkowska Michaela Slusarczyk Peter Staniszewski Ruth Erin Williams Suvi Ruokamo Tom Bear

TRY plant trait database – enhanced coverage and open access

Plant traits—the morphological, anatomical, physiological, biochemical and phenological characteristics of plants—determine how plants respond to environmental factors, affect other trophic levels, and influence ecosystem properties and their benefits and detriments to people. Plant trait data thus represent the basis for a vast area of research spanning from evolutionary biology, community and functional ecology, to biodiversity conservation, ecosystem and landscape management, restoration, biogeography and earth system modelling. Since its foundation in 2007, the TRY database of plant traits has grown continuously. It now provides unprecedented data coverage under an open access data policy and is the main plant trait database used by the research community worldwide. Increasingly, the TRY database also supports new frontiers of trait-based plant research, including the identification of data gaps and the subsequent mobilization or measurement of new data. To support this development, in this article we evaluate the extent of the trait data compiled in TRY and analyse emerging patterns of data coverage and representativeness. Best species coverage is achieved for categorical traits—almost complete coverage for ‘plant growth form’. However, most traits relevant for ecology and vegetation modelling are characterized by continuous intraspecific variation and trait–environmental relationships. These traits have to be measured on individual plants in their respective environment. Despite unprecedented data coverage, we observe a humbling lack of completeness and representativeness of these continuous traits in many aspects. We, therefore, conclude that reducing data gaps and biases in the TRY database remains a key challenge and requires a coordinated approach to data mobilization and trait measurements. This can only be achieved in collaboration with other initiatives.Rest of authors: Decky Junaedi, Robert R. Junker, Eric Justes, Richard Kabzems, Jeffrey Kane, Zdenek Kaplan, Teja Kattenborn, Lyudmila Kavelenova, Elizabeth Kearsley, Anne Kempel, Tanaka Kenzo, Andrew Kerkhoff, Mohammed I. Khalil, Nicole L. Kinlock, Wilm Daniel Kissling, Kaoru Kitajima, Thomas Kitzberger, Rasmus Kjøller, Tamir Klein, Michael Kleyer, Jitka Klimešová, Joice Klipel, Brian Kloeppel, Stefan Klotz, Johannes M. H. Knops, Takashi Kohyama, Fumito Koike, Johannes Kollmann, Benjamin Komac, Kimberly Komatsu, Christian König, Nathan J. B. Kraft, Koen Kramer, Holger Kreft, Ingolf Kühn, Dushan Kumarathunge, Jonas Kuppler, Hiroko Kurokawa, Yoko Kurosawa, Shem Kuyah, Jean-Paul Laclau, Benoit Lafleur, Erik Lallai, Eric Lamb, Andrea Lamprecht, Daniel J. Larkin, Daniel Laughlin, Yoann Le Bagousse-Pinguet, Guerric le Maire, Peter C. le Roux, Elizabeth le Roux, Tali Lee, Frederic Lens, Simon L. Lewis, Barbara Lhotsky, Yuanzhi Li, Xine Li, Jeremy W. Lichstein, Mario Liebergesell, Jun Ying Lim, Yan-Shih Lin, Juan Carlos Linares, Chunjiang Liu, Daijun Liu, Udayangani Liu, Stuart Livingstone, Joan Llusià, Madelon Lohbeck, Álvaro López-García, Gabriela Lopez-Gonzalez, Zdeňka Lososová, Frédérique Louault, Balázs A. Lukács, Petr Lukeš, Yunjian Luo, Michele Lussu, Siyan Ma, Camilla Maciel Rabelo Pereira, Michelle Mack, Vincent Maire, Annikki Mäkelä, Harri Mäkinen, Ana Claudia Mendes Malhado, Azim Mallik, Peter Manning, Stefano Manzoni, Zuleica Marchetti, Luca Marchino, Vinicius Marcilio-Silva, Eric Marcon, Michela Marignani, Lars Markesteijn, Adam Martin, Cristina Martínez-Garza, Jordi Martínez-Vilalta, Tereza Mašková, Kelly Mason, Norman Mason, Tara Joy Massad, Jacynthe Masse, Itay Mayrose, James McCarthy, M. Luke McCormack, Katherine McCulloh, Ian R. McFadden, Brian J. McGill, Mara Y. McPartland, Juliana S. Medeiros, Belinda Medlyn, Pierre Meerts, Zia Mehrabi, Patrick Meir, Felipe P. L. Melo, Maurizio Mencuccini, Céline Meredieu, Julie Messier, Ilona Mészáros, Juha Metsaranta, Sean T. Michaletz, Chrysanthi Michelaki, Svetlana Migalina, Ruben Milla, Jesse E. D. Miller, Vanessa Minden, Ray Ming, Karel Mokany, Angela T. Moles, Attila Molnár V, Jane Molofsky, Martin Molz, Rebecca A. Montgomery, Arnaud Monty, Lenka Moravcová, Alvaro Moreno-Martínez, Marco Moretti, Akira S. Mori, Shigeta Mori, Dave Morris, Jane Morrison, Ladislav Mucina, Sandra Mueller, Christopher D. Muir, Sandra Cristina Müller, François Munoz, Isla H. Myers-Smith, Randall W. Myster, Masahiro Nagano, Shawna Naidu, Ayyappan Narayanan, Balachandran Natesan, Luka Negoita, Andrew S. Nelson, Eike Lena Neuschulz, Jian Ni, Georg Niedrist, Jhon Nieto, Ülo Niinemets, Rachael Nolan, Henning Nottebrock, Yann Nouvellon, Alexander Novakovskiy, The Nutrient Network, Kristin Odden Nystuen, Anthony O'Grady, Kevin O'Hara, Andrew O'Reilly-Nugent, Simon Oakley, Walter Oberhuber, Toshiyuki Ohtsuka, Ricardo Oliveira, Kinga Öllerer, Mark E. Olson, Vladimir Onipchenko, Yusuke Onoda, Renske E. Onstein, Jenny C. Ordonez, Noriyuki Osada, Ivika Ostonen, Gianluigi Ottaviani, Sarah Otto, Gerhard E. Overbeck, Wim A. Ozinga, Anna T. Pahl, C. E. Timothy Paine, Robin J. Pakeman, Aristotelis C. Papageorgiou, Evgeniya Parfionova, Meelis Pärtel, Marco Patacca, Susana Paula, Juraj Paule, Harald Pauli, Juli G. Pausas, Begoña Peco, Josep Penuelas, Antonio Perea, Pablo Luis Peri, Ana Carolina Petisco-Souza, Alessandro Petraglia, Any Mary Petritan, Oliver L. Phillips, Simon Pierce, Valério D. Pillar, Jan Pisek, Alexandr Pomogaybin, Hendrik Poorter, Angelika Portsmuth, Peter Poschlod, Catherine Potvin, Devon Pounds, A. Shafer Powell, Sally A. Power, Andreas Prinzing, Giacomo Puglielli, Petr Pyšek, Valerie Raevel, Anja Rammig, Johannes Ransijn, Courtenay A. Ray, Peter B. Reich, Markus Reichstein, Douglas E. B. Reid, Maxime Réjou-Méchain, Victor Resco de Dios, Sabina Ribeiro, Sarah Richardson, Kersti Riibak, Matthias C. Rillig, Fiamma Riviera, Elisabeth M. R. Robert, Scott Roberts, Bjorn Robroek, Adam Roddy, Arthur Vinicius Rodrigues, Alistair Rogers, Emily Rollinson, Victor Rolo, Christine Römermann, Dina Ronzhina, Christiane Roscher, Julieta A. Rosell, Milena Fermina Rosenfield, Christian Rossi, David B. Roy, Samuel Royer-Tardif, Nadja Rüger, Ricardo Ruiz-Peinado, Sabine B. Rumpf, Graciela M. Rusch, Masahiro Ryo, Lawren Sack, Angela Saldaña, Beatriz Salgado-Negret, Roberto Salguero-Gomez, Ignacio Santa-Regina, Ana Carolina Santacruz-García, Joaquim Santos, Jordi Sardans, Brandon Schamp, Michael Scherer-Lorenzen, Matthias Schleuning, Bernhard Schmid, Marco Schmidt, Sylvain Schmitt, Julio V. Schneider, Simon D. Schowanek, Julian Schrader, Franziska Schrodt, Bernhard Schuldt, Frank Schurr, Galia Selaya Garvizu, Marina Semchenko, Colleen Seymour, Julia C. Sfair, Joanne M. Sharpe, Christine S. Sheppard, Serge Sheremetiev, Satomi Shiodera, Bill Shipley, Tanvir Ahmed Shovon, Alrun Siebenkäs, Carlos Sierra, Vasco Silva, Mateus Silva, Tommaso Sitzia, Henrik Sjöman, Martijn Slot, Nicholas G. Smith, Darwin Sodhi, Pamela Soltis, Douglas Soltis, Ben Somers, Grégory Sonnier, Mia Vedel Sørensen, Enio Egon Sosinski Jr, Nadejda A. Soudzilovskaia, Alexandre F. Souza, Marko Spasojevic, Marta Gaia Sperandii, Amanda B. Stan, James Stegen, Klaus Steinbauer, Jörg G. Stephan, Frank Sterck, Dejan B. Stojanovic, Tanya Strydom, Maria Laura Suarez, Jens-Christian Svenning, Ivana Svitková, Marek Svitok, Miroslav Svoboda, Emily Swaine, Nathan Swenson, Marcelo Tabarelli, Kentaro Takagi, Ulrike Tappeiner, Rubén Tarifa, Simon Tauugourdeau, Cagatay Tavsanoglu, Mariska te Beest, Leho Tedersoo, Nelson Thiffault, Dominik Thom, Evert Thomas, Ken Thompson, Peter E. Thornton, Wilfried Thuiller, Lubomír Tichý, David Tissue, Mark G. Tjoelker, David Yue Phin Tng, Joseph Tobias, Péter Török, Tonantzin Tarin, José M. Torres-Ruiz, Béla Tóthmérész, Martina Treurnicht, Valeria Trivellone, Franck Trolliet, Volodymyr Trotsiuk, James L. Tsakalos, Ioannis Tsiripidis, Niklas Tysklind, Toru Umehara, Vladimir Usoltsev, Matthew Vadeboncoeur, Jamil Vaezi, Fernando Valladares, Jana Vamosi, Peter M. van Bodegom, Michiel van Breugel, Elisa Van Cleemput, Martine van de Weg, Stephni van der Merwe, Fons van der Plas, Masha T. van der Sande, Mark van Kleunen, Koenraad Van Meerbeek, Mark Vanderwel, Kim André Vanselow, Angelica Vårhammar, Laura Varone, Maribel Yesenia Vasquez Valderrama, Kiril Vassilev, Mark Vellend, Erik J. Veneklaas, Hans Verbeeck, Kris Verheyen, Alexander Vibrans, Ima Vieira, Jaime Villacís, Cyrille Violle, Pandi Vivek, Katrin Wagner, Matthew Waldram, Anthony Waldron, Anthony P. Walker, Martyn Waller, Gabriel Walther, Han Wang, Feng Wang, Weiqi Wang, Harry Watkins, James Watkins, Ulrich Weber, James T. Weedon, Liping Wei, Patrick Weigelt, Evan Weiher, Aidan W. Wells, Camilla Wellstein, Elizabeth Wenk, Mark Westoby, Alana Westwood, Philip John White, Mark Whitten, Mathew Williams, Daniel E. Winkler, Klaus Winter, Chevonne Womack, Ian J. Wright, S. Joseph Wright, Justin Wright, Bruno X. Pinho, Fabiano Ximenes, Toshihiro Yamada, Keiko Yamaji, Ruth Yanai, Nikolay Yankov, Benjamin Yguel, Kátia Janaina Zanini, Amy E. Zanne, David Zelený, Yun-Peng Zhao, Jingming Zheng, Ji Zheng, Kasia Ziemińska, Chad R. Zirbel, Georg Zizka, Irié Casimir Zo-Bi, Gerhard Zotz, Christian Wirth.Max Planck Institute for Biogeochemistry; Max Planck Society; German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig; International Programme of Biodiversity Science (DIVERSITAS); International Geosphere-Biosphere Programme (IGBP); Future Earth; French Foundation for Biodiversity Research (FRB); GIS ‘Climat, Environnement et Société'.http://wileyonlinelibrary.com/journal/gcbhj2021Plant Production and Soil Scienc

EFFECTIVENESS OF THE USE OF INFRARED AND ULTRASOUND VASCULAR IMAGE DEVICES IN THE PERIPHERAL VENOUS ACCESS CHANNEL COMPARED TO TRADITIONAL TECHNIQUE TO REDUCE THE NUMBER OF PUNCTURE ATTEMPTS IN PEDIATRIC PATIENTS

Trabajo AcadémicoOBJETIVO: Sistematizar la evidencia sobre la efectividad del uso de dispositivos de imagen vascular infrarrojo y ultrasonido en la canalización de acceso venoso periférico comparado con la técnica tradicional para reducir el número de intentos de punción en pacientes pediátricos. MATERIAL Y MÉTODO: Revisión sistemática de 10 artículos científicos indizados en bases de datos: Lilacs y PubMed. Analizados según escala Grade. RESULTADOS: El 60% de las evidencias coinciden que el uso de dichos dispositivos mejoro la tasa de éxito en la canalización. CONCLUSIONES: 6 de 10 artículos, demuestran la efectividad del uso de dispositivos de imagen vascular: infrarrojo y ultrasonido y 4 de 10 artículos señala que no supera la técnica de canalización tradicional y/o no presenta resultados significativo

The Impacts of Mental and Physical Health on Aging

Over the last few decades, there has been a research emphasis on studying both risk and protective factors for abnormal aging. Risk factors increase one’s likelihood of developing a disease while protective factors antagonize that and decrease one’s likelihood of developing a disease. For example, physical activity has been shown to improve mental health (Craft & Perna, 2004). Meanwhile, a lack of physical activity can be a risk factor for both poor mental health and poor physical health long term, specifically in the older adult population (Blondell et al., 2014). Leisure time physical activity is recommended for all age groups, but as age increases, people begin to struggle more with disabilities, lack of time, lack of motivation, etc. that can keep them from regularly exercising. This study is designed to determine the impact that the amount of leisure time physical activity has on the mental health of older adults. We hypothesized that no leisure time physical activity and poor physical health would each correlate positively with poor mental health and with depression. We also hypothesized that no leisure time physical activity and poor physical health would positively correlate. Data were obtained from mySidewalk. mySidewalk is a platform that helps organize and share health data with the community. Specific variables of interest extracted from the database included: poor mental health among adults, diagnosed depression among adults, poor physical health among adults, and no leisure time physical activity level data. Data were aggregated at the county level. We found support for our hypotheses that poor physical health and low levels of leisure time physical activity both correlated positively with poor mental health. We also found that no leisure time physical activity correlated positively with poor physical health, which supported our other hypothesis. These results stress the importance of encouraging healthy behaviors and spreading awareness in regard to the various risk factors that can influence abnormal aging

Estudio de la percepción social en el entorno del Parque Natural de las Dunas de Corralejo (Fuerteventura, Islas Canarias): aplicaciones para la gestión integrada de zonas costeras

El Parque Natural de Corralejo y su entorno inmediato presentan un sistema de dunas transgresivo que, asociado a un conjunto de playas de arenas organógenas, caracterizan el extremo nororiental de la isla de Fuerteventura (término municipal de La Oliva). La existencia de este sistema playa‐duna, unido a otros factores naturales y socioeconómicos, ha sido clave para el desarrollo del turismo de masas en esta zona. Este proceso se inicia en la década de los setenta del pasado siglo, pero a partir de la década ochenta el crecimiento urbano‐turístico ha sido expectacular. De la interacción entre las actividades turísticas y los procesos naturales característicos de este sistema se ha derivado una problemática ambiental diversa. Ésta evidencia actualmente un conflicto entre las funciones de conservación y de protección del sistema de dunas, y los usos recreativos asociados al turismo masivo de sol y playa. En esta disyuntiva, se requieren nuevas fórmulas de gestión que favorezcan la multifuncionalidad del sistema, que no comprometan su sostenibilidad (tanto socio‐económica como ambiental). Es precisamente ésta una de las líneas que se abordan en el proyecto “Diagnóstico ambiental de los sistemas de dunas de Canarias para la elaboración de modelos sostenibles de gestión territorial” (DUNCAN), financiado por el Ministerio de Ciencia e Innovación y fondos FEDER (CSO2010‐18150). A través de este proyecto se pretende abordar un diagnóstico participativo, con el fin de conocer la percepción de la problemática ambiental de los diferentes actores de este espacio de dunas litorales, así como favorecer su sensibilización y estimular a los colectivos para la formulación de propuestas. En este contexto, la presente comunicación avanza los primeros resultados obtenidos en un estudio de percepción social, aplicado en el ámbito local y dirigido a los principales agentes del ámbito institucional, representantes de sectores socioeconómicos (turismo, restauración, pesca, pastoreo…) y entidades ambientalistas. Para ello se ha utilizado una entrevista semi‐estructurada, realizada a mediados de 2011 a más de una quincena de actores claves, a través de la cual se han sondeado cuestiones como el grado de conocimiento del sistema sedimentario eólico, las características del modelo turístico desarrollado en Corralejo o sus consecuencias ambientales. Los resultados preliminares desvelan el escaso conocimiento local de la problemática ambiental que afecta al sistema de dunas. Además, también se ha podido detectar la diversidad de preferencias y demandas sobre las posibles estrategias de gestión y, con ello, prever los puntos de conflicto que puede surgir en su implementación

Estudio de la percepción social en el entorno del Parque Natural de las Dunas de Corralejo (Fuerteventura, Islas Canarias): aplicaciones para la gestión integrada de zonas costeras

El Parque Natural de Corralejo y su entorno inmediato presentan un sistema de dunas transgresivo que, asociado a un conjunto de playas de arenas organógenas, caracterizan el extremo nororiental de la isla de Fuerteventura (término municipal de La Oliva). La existencia de este sistema playa‐duna, unido a otros factores naturales y socioeconómicos, ha sido clave para el desarrollo del turismo de masas en esta zona. Este proceso se inicia en la década de los setenta del pasado siglo, pero a partir de la década ochenta el crecimiento urbano‐turístico ha sido expectacular. De la interacción entre las actividades turísticas y los procesos naturales característicos de este sistema se ha derivado una problemática ambiental diversa. Ésta evidencia actualmente un conflicto entre las funciones de conservación y de protección del sistema de dunas, y los usos recreativos asociados al turismo masivo de sol y playa. En esta disyuntiva, se requieren nuevas fórmulas de gestión que favorezcan la multifuncionalidad del sistema, que no comprometan su sostenibilidad (tanto socio‐económica como ambiental). Es precisamente ésta una de las líneas que se abordan en el proyecto “Diagnóstico ambiental de los sistemas de dunas de Canarias para la elaboración de modelos sostenibles de gestión territorial” (DUNCAN), financiado por el Ministerio de Ciencia e Innovación y fondos FEDER (CSO2010‐18150). A través de este proyecto se pretende abordar un diagnóstico participativo, con el fin de conocer la percepción de la problemática ambiental de los diferentes actores de este espacio de dunas litorales, así como favorecer su sensibilización y estimular a los colectivos para la formulación de propuestas. En este contexto, la presente comunicación avanza los primeros resultados obtenidos en un estudio de percepción social, aplicado en el ámbito local y dirigido a los principales agentes del ámbito institucional, representantes de sectores socioeconómicos (turismo, restauración, pesca, pastoreo…) y entidades ambientalistas. Para ello se ha utilizado una entrevista semi‐estructurada, realizada a mediados de 2011 a más de una quincena de actores claves, a través de la cual se han sondeado cuestiones como el grado de conocimiento del sistema sedimentario eólico, las características del modelo turístico desarrollado en Corralejo o sus consecuencias ambientales. Los resultados preliminares desvelan el escaso conocimiento local de la problemática ambiental que afecta al sistema de dunas. Además, también se ha podido detectar la diversidad de preferencias y demandas sobre las posibles estrategias de gestión y, con ello, prever los puntos de conflicto que puede surgir en su implementación

CTNI-51. FEASIBILITY AND SAFETY OF NEUROSURGICAL RESECTION AND INTRA-OPERATIVE RADIATION THERAPY USING THE XOFT AXXENT ELECTRONIC BRACHYTHERAPY SYSTEM AND BEVACIZUMAB IN RECURRENT GLIOBLASTOMA

Glioblastoma (GBM) is the most common and aggressive primary brain tumor. External beam radiation therapy (EBRT) is widely recognized as an effective treatment for primary GBM. EBRT applied after GBM resection is associated with an increase in overall survival. Beyond upfront therapy with radiation and temozolomide chemotherapy, there is no standard therapy that has been proven effective. Re-irradiation with ERBT is minimally effective and with significant comorbidities. Our study uses IORT at the time of re-resection in patients who have recurrent, operable GBM after failure of who received primary EBRT and temozolamide in their first course of treatment. IORT allows the delivery of a large effective radiation dose applied directly to the tumor bed at the time of resection. This affords direct radiation to be delivered to the micro- and macroscopic tumor remnants in the vicinity of the resection cavity immediately following gross resection. In contrast, the more distant, surrounding brain tissue does not receive high radiation exposure. This Phase II study continues to accrue subjects with the primary endpoint of overall survival and several secondary endpoints that includes progression free survival, quality of life, and adverse events/safety. To date, five patients have been treated on this protocol. The technical feasibility and safety of administering intraoperative radiation with the Xoft System has been established in this cohort. Preliminary GLIOX Trial findings demonstrate the safety and feasibility of using the Xoft Axxent® Electronic Brachytherapy System to administer intracranial radiation during resection of recurrent glioblastomas. We will report new and expansive study results to include these early findings

Response to OKN-007 and NAC in a Patient with Unilateral Hearing Loss and Chronic Tinnitus from Vestibular Schwannoma

Background: Tinnitus is the perception of sound in the absence of external acoustic stimulation. Being one of the most common diseases of the ear, it has a global prevalence ranging from 4.1 to 37.2%. To date, it has been difficult to treat tinnitus as its pathophysiology is poorly understood and there are limited treatment options. Objective: To investigate the effect of OKN-007 (also known as HPN-07), a nitrone-based investigational drug, in combination with oral N-acetylcycsteine (NAC), for the treatment of hearing loss and chronic tinnitus under an individual expanded access protocol. Patient Case: We report the case of a patient who presented with left-sided ear fullness, mild tinnitus, and mild high frequency sensorineural hearing loss with 100% word recognition. A large enhancing mass seen on MRI revealed a vestibular schwannoma. He underwent subtotal resection of the tumor resulting in a moderate-to-profound sensorineural hearing loss and catastrophic tinnitus. The patient was treated with intravenous OKN-007 at 60 mg/kg dosed three times per week and oral NAC 2500 mg twice daily. Results: Post-treatment audiometric testing revealed an average of 16.66 dB in hearing threshold improvement in three frequencies (125, 250 and 500 Hz) with residual hearing in the affected left ear. His tinnitus loudness matching improved from 90 dB to 19 dB post-treatment. His Tinnitus Handicap Inventory improved from 86/100 (Catastrophic) to 40/100 (Moderate). He also experienced improvements in sleep, concentration, hearing, and emotional well-being, and reported significantly decreased levels of tinnitusrelated distress. Conclusions: This case report highlights the feasibility and therapeutic potential of the combination of OKN-007 and NAC in treating hearing loss and tinnitus that warrants further investigation. Keywords: Tinnitus, Hearing loss, OKN-007

BETA PRIME: Phase I study of AdAPT-001 as monotherapy and combined with a checkpoint inhibitor in superficially accessible, treatment-refractory solid tumors.

AdAPT-001 is an investigational therapy consisting of a replicative type 5 adenovirus armed with a TGF-β receptor-immunoglobulin Fc fusion trap, designed to neutralize isoforms 1 and 3 of the profibrotic and immunosuppressive cytokine, TGF-β. In preclinical studies with an immunocompetent mouse model, AdAPT-001 eradicated directly treated \u27cold\u27 tumors as well as distant untreated tumors, and, from its induction of systemic CD8+ T cell-mediated antitumor immunity, protected the mice from rechallenge with tumor cells. AdAPT-001 also sensitized resistant tumors to checkpoint blockade. This manuscript describes the rationale and design of the first-in-human phase I, dose-escalation and dose-expansion study of AdAPT-001 alone and in combination with a checkpoint inhibitor in adults with treatment-refractory superficially accessible solid tumors. Trial registration: ClinicalTrials.gov NCT04673942. Keywords: AdAPT-001; BETA PRIME; TGF-β trap; abscopal effect; oncolytic adenovirus; solid tumors; study protocol

Real-time assay for monitoring membrane association of lipid-binding domains

The C2 domain is a common protein module which mediates calcium-dependent phospholipid binding. Several assays have previously been developed to measure membrane association. However, these assays either have technical drawbacks or are laborious to carry out. We now present a simple solution-based turbidity method for rapidly assaying membrane association of single lipid-binding domains in real time. We used the first C2 domain of synaptotagmin1 (C2A) as a model lipid-binding moiety. Our use of the common dimeric glutathione-S-transferase (GST) fusion tag allowed two C2A domains to be brought into close proximity. Consequently, calcium-triggered phospholipid binding by this artificially dimerized C2A resulted in liposomal aggregation, easily assayed by following absorbance of the solution at 350 nm. The assay is simple and sensitive and can be scaled up conveniently for use in a multiwell plate format, allowing high-throughput screening. In our screens, we identified nickel as a novel activator of synaptotagmin1 C2A domain membrane association. Finally, we show that the turbidity method can be applied to the study of other GST-tagged lipid-binding proteins such as epsin, protein kinase C-β, and synaptobrevin