Relationship between sex hormone levels, bone mineral density and bone turnover markers in healthy moroccan men: a cross-sectional study

Introduction: Gonadal steroid hormones play a crucial role during skeletal growth and maturation in  both men and women. The aim of this study is to evaluate the relationship of sex hormone levels, bone mineral density and biochemical markers of bone turnover in healthy Moroccan men.Methods: 142 Moroccan men who had no previous diagnosis of osteoporosis were enrolled prospectively in this cross-sectional study between December 2009 and August 2010. Also, subjects were excluded from the study if they had conditions affecting bone metabolism. Different biochemical parameters were assayed: Testosterone, Estradiol, sex hormone binding globulin, Osteocalcin, vitamin D, crosslaps,  intact parathyroid hormone and alkaline phosphatase. Dual-energy X-ray absorptiometry was used to measure the Bone mineral density (BMD) (g/cm2).Results: In this study, among the 142 Moroccan men, 29 (20.1%) had densitometry osteoporosis and the  prevalence of vitamin D insufficiency was 94%. No corrolation was found between Estradiol,  Testosterone and bone mineral density but we found significant  differences in the levels of Estradiol between patients with osteoporosis, osteopenia and normal patients. Bone mineral density at the lumbar spine was negatively correlated to hormone-binding globulin and positively correlated to free androgen index, free estrogen index and the Body mass index. BMD at the total hip was positively correlated to free androgen index, Body mass index and negatively correlated to sex hormone binding globulin, alkaline phosphatase, intact parathyroid hormone, osteocalcin, Crosslaps and age. Conclusion: Our study showed that increasing age, intact parathyroid hormone and alkaline phosphatase levels and decreasing body mass index were the most important independent factors associated to the presence of a low BMD at the total hip. Increasing body mass index and free androgen index level were the most important independent factors associated to the presence of a low BMD at the lumbar spine. The combination of variable that best predicted the male osteoporosis is age, body mass index, alkaline phosphatase and cigarette  smoking.Key words: Male osteoporosis, sex hormones, vitamin D, Bone remodeling marker

COMPARATIVE STUDY OF THE EFFICACY OF STEM CELLS IN CORNEAL REGENERATION IN A CHEMICAL BURN IN RABBITS

Objectives: This study compares the efficacy of stem cell transplantation in corneal regeneration and restoration of the limbic deficit in an experimental chemical burn in rabbits. Methods: Biopsy was performed of the limbus and the chemical burns for all rabbits, and we collected the amniotic membranes from a pregnant female rabbit. We kept a control group without transplantation, to study spontaneous and natural healing, and we transplanted the stem cells produced in vitro under the corneal epithelium burned. To compare the result, we tested a group for amniotic stem cell transplantation, a group for limbal stem cell graft, and another group for combined transplantation of both types of stem cells. Results: Transplanted rabbits develop permanent unilateral blindness due to a severe limbic deficit. The group receiving only amniotic stem cells shows temporary anatomical improvement without functional recovery. The two groups receiving limbal stem cells alone or combined with amniotic stem cells showed anatomical and functional satisfaction with quick recovery time for the combined transplantation. Conclusions: A simple chemical burn can establish permanent blindness. When the limbic deficit is important, spontaneous healing is not available. Transplantation of stem cell transplant is the only way to repair this deficit and regenerate the cornea. Only limbic stem cells can be sufficient. Amniotic stem cells can support and speed up the healing time when it combined to limbal stem cells graft.               Peer Review History: Received 23 July 2020; Revised 14 August; Accepted 28 August, Available online 15 September 2020 Academic Editor: Essam Mohamed Eissa, Beni-Suef University, Egypt, [email protected] UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file:                Reviewer's Comments: Average Peer review marks at initial stage: 5.0/10 Average Peer review marks at publication stage: 7.5/10 Reviewer(s) detail: Dr. Mohamed Amin El-Emam, Department of Pharmacology and Therapeutics, Faculty of Pharmacy and Drug Manufacturing, Pharos University in Alexandria (PUA), Alexandria, Egypt, [email protected] Francesco Ferrara,USL Umbria 1, Perugia, Italy, [email protected] Maged Almezgagi, Department of Immunology, Medical College of Qinghai University, Qinghai Xining 810001, China, [email protected] Dr. Asia Selman Abdullah, University of Basrah, Iraq, [email protected]

Undiagnosed Phenylketonuria Can Exist Everywhere:Results From an International Survey

Many countries do not have a newborn screening (NBS) program, and immigrants from such countries are at risk for late diagnosis of phenylketonuria (PKU). In this international survey, 52 of 259 patients (20%) with late diagnosed PKU were immigrants, and 145 of the 259 (55%) were born before NBS or in a location without NBS

Undiagnosed Phenylketonuria Can Exist Everywhere: Results From an International Survey

Many countries do not have a newborn screening (NBS) program, and immigrants from such countries are at risk for late diagnosis of phenylketonuria (PKU). In this international survey, 52 of 259 patients (20%) with late diagnosed PKU were immigrants, and 145 of the 259 (55%) were born before NBS or in a location without NBS

Guillain-Barré Syndrome, Greater Paris Area

We studied 263 cases of Guillain-Barré syndrome from 1996 to 2001, 40% of which were associated with a known causative agent, mainly Campylobacter jejuni (22%) or cytomegalovirus (15%). The cases with no known agent (60%) peaked in winter, and half were preceded by respiratory infection, influenzalike syndrome, or gastrointestinal illness

An autosomal recessive leucoencephalopathy with ischemic stroke, dysmorphic syndrome and retinitis pigmentosa maps to chromosome 17q24.2-25.3

Background Single-gene disorders related to ischemic stroke seem to be an important cause of stroke in young patients without known risk factors. To identify new genes responsible of such diseases, we studied a consanguineous Moroccan family with three affected individuals displaying hereditary leucoencephalopathy with ischemic stroke, dysmorphic syndrome and retinitis pigmentosa that appears to segregate in autosomal recessive pattern. Methods All family members underwent neurological and radiological examinations. A genome wide search was conducted in this family using the ABI PRISM linkage mapping set version 2.5 from Applied Biosystems. Six candidate genes within the region linked to the disease were screened for mutations by direct sequencing. Results Evidence of linkage was obtained on chromosome 17q24.2-25.3. Analysis of recombination events and LOD score calculation suggests linkage of the responsible gene in a genetic interval of 11 Mb located between D17S789 and D17S1806 with a maximal multipoint LOD score of 2.90. Sequencing of seven candidate genes in this locus, ATP5H, FDXR, SLC25A19, MCT8, CYGB, KCNJ16 and GRIN2C, identified three missense mutations in the FDXR gene which were also found in a homozygous state in three healthy controls, suggesting that these variants are not disease-causing mutations in the family. Conclusion A novel locus for leucoencephalopathy with ischemic stroke, dysmorphic syndrome and retinitis pigmentosa has been mapped to chromosome 17q24.2-25.3 in a consanguineous Moroccan family

The Comic Phenomenon in Hebrew Bible Narrative and Its Implications for Feminist Hermeneutics

EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Variabilité de l’hélianthinine isolée à partir de graines de lignées de tournesol à faible et à haute teneur en acide oléique

The helianthinin is a major stock protein of the Sunflower seed. The polypeptides composition of helianthinin is heterogeneous and corresponded to the family gene heterogeneity. The SDS electrophoresis gel polyacrylamide analyses of total fractions of proteins extracted from 24 of Sunflower genotypes provided from INRA of Montpellier have revealed a variable helianthinin polypeptide. The electrophoretic analysis revealed specific polypeptide of wild genotypes, hybrids, normal and high oleic acid breeding line. These results show six variables polypeptides of helianthinin with molecular weight of PM 43,8 kD, 43, 5 kD, 43,3 kD, 27, 2 kD, 26,4 kD et 24,6 kD. The HOC genotype has three polypeptides of 43,8 kD, 43,3 kD and 24,6 kD and corresponds to the hybrid H. Argophyllus x H. Annus. The Vniimk population Russe and the Armavir population with normal oleic acid have a similar helianthinin electrophoretic profile.Les sources de protéines végétales représentent une place importante dans les pays en voie de développement et compensent une alimentation dominée par les glucides telles que les céréales et autres. Les sources de protéines végétales sont moins chères et constituent des matières azotées utilisées pour l’alimentation animale. Les graines de Tournesol sont une source de matière première recherchée pour l’alimentation humaine et animale. La composition des graines de Tournesol est largement influencée par les facteurs génétiques et environnentaux. Ce travail a pour objectif la comparaison des protéines de réserve majeures de Tournesol après fractionnement e en Albumines et Globulines et analysées en SDS PAGE chez les variétés locales et sélectionnées par l’INRA de Montpelier. L’hélianthine est une protéine majeure de réserve au niveau des graines de Tournesol. La composition en polypeptides de l’hélianthine est hétérogène due à l’hétérogénéité des familles de gènes correspondants. L’analyse électrophorétique sur gel de polyacrylamide en présence de SDS des fractions de polypeptides isolées à partir de graines de 24 génotypes de Tournesol obtenus par l’INRA de Montpelier ont révélé des polypeptides de l’hélianthinine de PM respectifs de de PM 43,8 kD, 43, 5 kD, 43,3 kD, 27, 2 kD, 26,4 kD et 24,6 kD. Ces polypeptides présentent des variabilités chez les génotypes sauvages, hybrides et lignées normales et à teneur élevée en acide oléique. Les génotypes caractérisés par une teneur élevée en acide oléique ont deux polypeptides de 43,8 kD, 43,3 kD, et ont le peptide de 24,6 kD et se rapprochent de l’hybride H. Argophyllus x H. Annuus. La population d‘origine Russe Vniimk et la population Russe Armavir présentant une teneur normale en acide oléique présentent un profil électrophorétique similaire