Antimicrobial Investigation and Synthesis of Novel 1H-Pyrazole-4- (5H)-yl Diazene Derivatives

Bu çalışmada, ilk olarak başlangıç maddesi olan etil 1-(4-bromofenil)-2-(1,3,5-trifenil-1H-pirazol-4-(5H)-ilden)hidrazinin (1) sentezi gerçekleştirildi. Elde edilen başlangıç maddesi çeşitli hidrazinlerle etkileştirilerekbir seri yeni 1H-pirazol-4-il diazen (2a-h) türevleri sentezlendi. Sentezlenen bileşikler elementel analiz, IR veNMR gibi spektral yöntemlerle karakterize edildi. Sentezlenen bileşiklerin antibakteriyel aktiviteleri Gram ()ve Gram (-) bakteriler üzerinde disk difüzyon yöntemi kullanılarak araştırıldı. Kontrol grubu olarak Gentamisin,Cefotaxime antibiyotikleri kullanılırken sentezlenen bileşiklerin kontrol grubu ile karşılaştırıldığında herhangi birdozun antibakteriyel aktivite göstermediği gözlendi.In this study, firstly, ethyl 1-(4-bromophenyl)-2-(1,3,5-triphenyl-1H-pyrazol-4-(5H)-ylidene)hydrazine (1), which has starting material, was synthesized. A series of new 1H-pyrazol-4-yl diazene (2a-h)derivatives were synthesized from reaction the obtained starting material and various hydrazines. The synthesizedcompounds were confirmed by spectral methods such as elemental analysis, IR and NMR. Antibacterial activity ofthe compounds were investigated by disc diffusion method against to Gram () and Gram (-) bacteria. Gentamicinand Cefotaxime antibiotics were used as the control group. None of the doses showed any antibacterial activitycompared to the control

Determination of seal coat deterioration using image processing methods

Seal coat is the most commonly used asphalt pavement type due to its low initial construction cost and ease of application in countries such as Turkey, Australia, South Africa and New Zealand. Seal coat deterioration occurs over time because of the effect of various factors such as weather, traffic, etc. The determination and assessment of deterioration is an important components of pavement management systems (PMS). This article presents, digital image processing (DIP) techniques as effective and reliable measurement techniques for the determination of bleeding deterioration in seal coats. The developed technique was applied to a total of 140 images, taken from four survey sites in four different Highway Districts. These images were obtained with an image acquisition device that was developed to take images for this study. Each image was classified in one of two categories, namely, bleeding or satisfactory. One hundred seal coat images were classified as bleeding surfaces and the others were satisfactory surfaces. The edge detection algorithm was developed using the image processing toolbox of Matlab software. Aggregate edge patterns of bleeding or satisfactory seal coat surfaces differ significantly. Therefore, in this study was examined the edges of aggregate particles using seal coat images. The results show that bleeding deterioration on seal coat was determined accurately using the developed algorithm in the scope of study. The results also indicate that this system is a promising tool in seal coat surface condition evaluation, potentially aiding pavement engineers in prioritizing seal coat projects in a quantitative rather than qualitative manner. (C) 2013 Elsevier Ltd. All rights reserved

EFFECT OF YAW ANGLE ON THE FORMATION OF VORTEX BREAKDOWN OVER THE DIAMOND WING

WOS: 000277481000009In this study, the flow structure close to the surface of the non slender diamond wing was investigated both qualitatively and quantitatively using dye visualization and the 3-D Particle Image Velocimetry (PIV) technique. The flow structure and formation of the vortex breakdown were studied by varying the yaw angle of the wing within the range of 0 degrees <=theta <=<15 degrees for the angle of attack of alpha=7 degrees and Reynolds number of 10 000. After a certain distance from the wing apex, a stagnation points and onset of vortex breakdowns took places on the central axes of spiral vortices that occurred over the wing surface for the angle of attack of alpha=7 degrees. When the yaw angle was increased the locations of vortex breakdowns approached to the wing apex, but, the other one moves towards the trailing edge

Effects of construction-related factors on chip seal performance

Chip seal is an economical type of asphalt pavement that is widely used in countries such as Turkey, Australia, South Africa and New Zealand. However, unlike hot mix asphalt pavements, the performance of this type of pavement can be affected by many different parameters. Although these parameters have been identified in the literature, the extent to which each of these parameters affects the performance of chip seal has not yet been determined. The leading parameters affecting chip seal performance are those related to construction, including the adhesion capability of the aggregate used in construction, natural dust amount of the aggregate, ambient temperature during construction, bitumen temperature in the bitumen distributor tanks, time between bitumen spraying and aggregate spreading, time between aggregate spreading and rolling, time between construction stages, surface temperature during construction, aggregate and bitumen designs, in situ plate test values and per cent embedment of the aggregates. To determine the effect of these construction parameters on pavement performance, they were observed and measured on five test roads. The chip-sealed test roads were monitored for 1 year with nondestructive tests and observations to determine which construction-related parameters affect the performance of the pavement. The study results indicated that macrotexture loss is the most important deterioration factor in chip-sealed pavements; initial texture depth is also important with respect to the long-term performance of chip-sealed pavements. Rolling operations is another important factor for the performance of chip seal. If rolling operations are performed at high ambient temperatures, the per cent embedment of the aggregate will increase, causing the chip seal to deteriorate more rapidly. Consequently, the ambient temperature during the construction of chip seal is important; thus, chip seal should not be constructed at ambient temperatures lower than 30 degrees C or higher than 43.5 degrees C. (C) 2012 Elsevier Ltd. All rights reserved

Effects of construction-related factors on chip seal performance

Chip seal is an economical type of asphalt pavement that is widely used in countries such as Turkey, Australia, South Africa and New Zealand. However, unlike hot mix asphalt pavements, the performance of this type of pavement can be affected by many different parameters. Although these parameters have been identified in the literature, the extent to which each of these parameters affects the performance of chip seal has not yet been determined. The leading parameters affecting chip seal performance are those related to construction, including the adhesion capability of the aggregate used in construction, natural dust amount of the aggregate, ambient temperature during construction, bitumen temperature in the bitumen distributor tanks, time between bitumen spraying and aggregate spreading, time between aggregate spreading and rolling, time between construction stages, surface temperature during construction, aggregate and bitumen designs, in situ plate test values and per cent embedment of the aggregates. To determine the effect of these construction parameters on pavement performance, they were observed and measured on five test roads. The chip-sealed test roads were monitored for 1 year with nondestructive tests and observations to determine which construction-related parameters affect the performance of the pavement. The study results indicated that macrotexture loss is the most important deterioration factor in chip-sealed pavements; initial texture depth is also important with respect to the long-term performance of chip-sealed pavements. Rolling operations is another important factor for the performance of chip seal. If rolling operations are performed at high ambient temperatures, the per cent embedment of the aggregate will increase, causing the chip seal to deteriorate more rapidly. Consequently, the ambient temperature during the construction of chip seal is important; thus, chip seal should not be constructed at ambient temperatures lower than 30 degrees C or higher than 43.5 degrees C. (C) 2012 Elsevier Ltd. All rights reserved

In vitro evaluation of the effects of lycopene on caspase system and oxidative DNA damage in high-glucose condition

WOS: 000466841900005Aim and Background: The present study was planned to investigate the effects of lycopene, on the caspase-dependent apoptosis in high-dose glucose (HG)-treated PC12 cell line. PC12 cells were cultured in vitro. Materials and Methods: HG was prepared as G (250 mM), and lycopene was prepared as L1 (10 mM), L2 (20 mM), and L3 (40 mM). After 6 h of incubation, the cells were exposed to trypsin, and the samples were obtained with freeze/thaw method. Caspase 3, 8, 9; 8-hydroxy-2-deoxyguanosine (8-OHdG); and M30 were determined (enzyme-linked immunosorbent assay). Results: 8-OHdG increased in L3 (P % 0.001), whereas L1 caused a decrease in HG group (P % 0.001). Caspase-3 decreased significantly in L1, L2, and L3G compared to control (P % 0.001) group. Caspase-8 increased significantly in L1, L1G, L2G, and all L3 glucose groups (P % 0.001). There was no difference for Caspase-9. M30 was not affected by L and HG, which decreased significantly (P % 0.001). Conclusion: As a result, it was determined that, when PC12 cell line was treated with HG, lycopene application had effects on caspase enzymes and DNA damage.Van Yuzuncu Yil University, Scientific Research Projects Unit [2015-SBE-YL094]This study was supported by the Van Yuzuncu Yil University, Scientific Research Projects Unit, registered with the project no: 2015-SBE-YL094

The Effect of Simvastatin on Pulmonary Damage in Experimental Peritonitis in Rats

Sahiner, Ibrahim Tayfun/0000-0002-3921-7675WOS: 000368531700037PubMed: 26730028Statins are widely used in the treatment of hyperlipidemia, as they inhibit cholesterol synthesis. They also have anti-inflammatory, antioxidant, immunomodulatory, and positive endothelial-functional effects. It is hypothesized that simvastatin ameliorates pulmonary damage secondary to peritonitis in rats. Forty Wistar albino rats were divided into four groups. In sham group, laparotomy was the standard procedure. In simvastatin group, simvastatin was given perorally before laparotomy. In sepsis group, peritoneal sepsis was constituted by cecal ligation and puncture technique. In sepsis+simvastatin group, the procedures of simvastatin and sepsis groups were applied together. After sacrification at the 72nd hour, tissue samples from lungs were harvested for histopathological examination, wet and dry weight measurements, and tissue culture, tissue malondialdehyde, and nitric oxide tests. Blood samples were taken for C-reactive protein and whole blood count. While the malondialdehyde levels were found to be significantly higher in sepsis group, nitric oxide levels were found to be significantly lower in simvastatin+sepsis group. Alveolar hemorrhage was highest in simvastatin+sepsis group. There was no difference for C-reactive protein, leukocyte levels, and histopathological examination between any groups. The ratios of wet and dry lung weights were higher in simvastatin-given groups. Simvastatin has no positive effect in terms of lung dysfunction on experimental sepsis model. For a better understanding of the effects of simvastatin on lung injury in peritoneal sepsis, experimental models of longer duration that enable to search the effects of simvastatin beyond 3 days will be more useful

Prolonged QRS independently predicts long-term all-cause mortality in patients with narrow QRS complex undergoing coronary artery bypass grafting surgery (9-year follow-up results)

WOS: 000574558500002PubMed: 33014085Aim: We investigated the association of intermediate QRS prolongation with the long-term all-cause mortality in coronary artery bypass grafting (CABG) surgery patients with a narrow QRS complex in the preoperative electrocardiography (ECG). Material and methods: A total of 221 consecutive patients with narrow QRS ( 89.5 ms determined all-cause mortality with a sensitivity of 73.7% and a specificity of 52% (OR = 2.07) due to ROC analysis. All-cause mortality was significantly higher in patients with preop QRS duration > 90 ms from the first year (chi(2) = 6.724, p = 0.010). Conclusions: in CABG patients with a narrow QRS complex, preoperative intermediate prolonged QRS is an independent predictor of all-cause mortality in long-term follow-up