Phosphorylation-independent activation of the atypical response regulator NblR

Cyanobacteria respond to environmental stress conditions by adjusting their photosynthesis machinery. In Synechococcus sp. PCC 7942, phycobilisome degradation and other acclimation responses after nutrient or high-light stress require activation by the orphan response regulator NblR, a member of the OmpR/PhoB family. Although NblR contains a putative phosphorylatable residue (Asp57), it lacks other conserved residues required to chelate the Mg2+ necessary for aspartic acid phosphorylation or to transduce the phosphorylation signal. In close agreement with these features, NblR was not phosphorylated in vitro by the low-molecular-mass phosphate donor acetyl phosphate and mutation of Asp57 to Ala had no impact on previously characterized NblR functions in Synechococcus. On the other hand, in vitro and in vivo assays show that the default state of NblR is monomeric, suggesting that, despite input differences, NblR activation could involve the same general mechanism of activation by dimerization present in known members of the OmpR/PhoB family. Structural and functional data indicate that the receiver domain of NblR shares similarities with other phosphorylation-independent response regulators such as FrzS and HP1043. To acknowledge the peculiarities of these atypical ‘two-component’ regulators with phosphorylation-independent signal transduction mechanisms, we propose the term PIARR, standing for phosphorylation-independent activation of response regulator.This work was supported by the Ministerio de Educación y Ciencia (grants BFU2006-12424 to A.C. and BIO2005-00153 to A. M.) and the Generalitat Valenciana (grant ACOMP06/083 to A.C.)

Actualización en náuseas y vómitos postoperatorios

Nausea and vomiting are common complications in the postoperative period. Patients who suffer this type of adverse events tend to perceive the health care received as deficient. In addition, postoperative nausea and vomiting pose a costly financial burden on the health system. The December 2020 issue of the journal Best Practice & Research Clinical Anaesthesiology carefully reviews current knowledge about postoperative nausea and vomiting (PONV). Knowledge of its pathophysiology is essential to understand the pharmacological targets used in its prevention and treatment. There are multiple families of drugs in the therapeutic arsenal against PONV. At present, the available evidence appears to relatively strongly support the use of a multimodal pharmacological approach. Risk assessment and stratification are useful to define the attitude towards a specific patient. Specific populations, such as pediatric or obstetric, should receive special considerations in the management of PONV. Furthermore, the significance of these complications beyond the time of hospital discharge should be considered. Regarding the current perspectives for the future, it is necessary to highlight both the incipient development of some new antiemetic drugs, as well as the growing role of personalized medicine through genetics.Las náuseas y los vómitos constituyen una complicación habitual en el periodo postoperatorio. Los pacientes que sufren este tipo de eventos adversos suelen percibir como deficiente la asistencia sanitaria recibida. Además, suponen una costosa carga económica sobre el sistema de salud. El ejemplar de Diciembre de 2020 de la revista Best Practice & Research Clinical Anaesthesiology revisa de manera minuciosa el conocimiento presente acerca de las náuseas y vómitos postoperatorios (NVPO). El conocimiento de su fisiopatología resulta esencial para comprender las dianas farmacológicas empleadas en su prevención y tratamiento. Existen múltiples familias de fármacos en el arsenal terapéutico contra las NVPO. En la actualidad, la evidencia disponible parece apoyar con relativa fuerza el empleo de un abordaje farmacológico multimodal. La evaluación y la estratificación del riesgo resultan de utilidad para definir la actitud ante un paciente determinado. Poblaciones concretas, como la pediátrica o la obstétrica, deben recibir consideraciones especiales en el abordaje de las NVPO. Además, debería considerarse la importancia de estas complicaciones más allá del momento del alta hospitalaria. En cuanto a las perspectivas actuales de cara al futuro, es preciso destacar tanto el desarrollo incipiente de algunos nuevos fármacos antieméticos, como el creciente papel de la medicina personalizada a través de la genética

Biochar Improves the Properties of Poultry Manure Compost as Growing Media for Rosemary Production

[EN] Compost represents a sustainable alternative for peat (P) replacement in soilless plant cultivation, but its use can be limited by several inadequate physical and physicochemical properties. Biochar can alleviate some of the limitations of compost for its use as growth media by improving the physical properties, decreasing salinity and making the phytotoxic compounds unavailable for plants. We studied the physical and physicochemical properties of holm oak biochar (B), poultry manure compost (PMC), poultry manure composted with biochar (PMBC), a commercial peat (P) and multiple combinations of these materials as growth media, and their effect on the rooting and growth of rosemary. PMBC and PMC showed similar physical and physicochemical properties as growing media, and they both were phytotoxic when used in a rate above 50% (by volume) in the growing medium. However, when used at proportion of 25%, PMBC was less phytotoxic than PMC and enhanced the percentage of rosemary cutting rooting. The incorporation of B in the growing medium instead of P (either at 50% or 75% in volume) increased the stability of the growing media and the percentage of rooted cuttings, but it did not affect plant growth significantly. Our results demonstrate the potential of substituting peat by a combination of poultry manure compost and biochar for the formulation of growth media.This research was funded by SPANISH MINISTRY OF ECONOMY AND COMPETITIVENESS, grant numbers AGL2012-40143-C02-01 and RTI2018-099417-B-I00, co-funded with EU FEDER fundsFornes Sebastiá, F.; Liu-Xu, L.; Lidón, A.; Sanchez-Garcia, M.; Luz Cayuela, M.; Sanchez-Monedero, MA.; Belda Navarro, RM. (2020). Biochar Improves the Properties of Poultry Manure Compost as Growing Media for Rosemary Production. 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Biological oxidation of elemental sulphur added to three composts from different feedstocks to reduce their pH for horticultural purposes. Bioresource Technology, 98(18), 3561-3569. doi:10.1016/j.biortech.2006.11.008Alburquerque, J. A., Gonzálvez, J., García, D., & Cegarra, J. (2006). Measuring detoxification and maturity in compost made from «alperujo», the solid by-product of extracting olive oil by the two-phase centrifugation system. Chemosphere, 64(3), 470-477. doi:10.1016/j.chemosphere.2005.10.055Wang, P., Changa, C. M., Watson, M. E., Dick, W. A., Chen, Y., & Hoitink, H. A. J. (2004). Maturity indices for composted dairy and pig manures. Soil Biology and Biochemistry, 36(5), 767-776. doi:10.1016/j.soilbio.2003.12.012Sáez, J. A., Belda, R. M., Bernal, M. P., & Fornes, F. (2016). Biochar improves agro-environmental aspects of pig slurry compost as a substrate for crops with energy and remediation uses. Industrial Crops and Products, 94, 97-106. doi:10.1016/j.indcrop.2016.08.035Kelleher, B. ., Leahy, J. ., Henihan, A. ., O’Dwyer, T. ., Sutton, D., & Leahy, M. . (2002). Advances in poultry litter disposal technology – a review. Bioresource Technology, 83(1), 27-36. doi:10.1016/s0960-8524(01)00133-xAtiyeh, R. M., Subler, S., Edwards, C. A., Bachman, G., Metzger, J. D., & Shuster, W. (2000). Effects of vermicomposts and composts on plant growth in horticultural container media and soil. Pedobiologia, 44(5), 579-590. doi:10.1078/s0031-4056(04)70073-6Steiner, C., & Harttung, T. (2014). Biochar as a growing media additive and peat substitute. Solid Earth, 5(2), 995-999. doi:10.5194/se-5-995-2014Woolf, D., Amonette, J. E., Street-Perrott, F. A., Lehmann, J., & Joseph, S. (2010). Sustainable biochar to mitigate global climate change. Nature Communications, 1(1). doi:10.1038/ncomms1053Fornes, F., & Belda, R. M. (2018). Biochar versus hydrochar as growth media constituents for ornamental plant cultivation. Scientia Agricola, 75(4), 304-312. doi:10.1590/1678-992x-2017-0062Tian, Y., Sun, X., Li, S., Wang, H., Wang, L., Cao, J., & Zhang, L. (2012). Biochar made from green waste as peat substitute in growth media for Calathea rotundifola cv. Fasciata. Scientia Horticulturae, 143, 15-18. doi:10.1016/j.scienta.2012.05.018Fornes, F., Belda, R. M., Fernández de Córdova, P., & Cebolla-Cornejo, J. (2017). Assessment of biochar and hydrochar as minor to major constituents of growing media for containerized tomato production. Journal of the Science of Food and Agriculture, 97(11), 3675-3684. doi:10.1002/jsfa.8227Petruccelli, R., Bonetti, A., Traversi, M. L., Faraloni, C., Valagussa, M., & Pozzi, A. (2015). Influence of biochar application on nutritional quality of tomato (Lycopersicon esculentum). Crop and Pasture Science, 66(7), 747. doi:10.1071/cp14247Belda, R. M., Lidón, A., & Fornes, F. (2016). Biochars and hydrochars as substrate constituents for soilless growth of myrtle and mastic. Industrial Crops and Products, 94, 132-142. doi:10.1016/j.indcrop.2016.08.024Fornes, F., & Belda, R. M. (2019). Use of raw and acidified biochars as constituents of growth media for forest seedling production. New Forests, 50(6), 1063-1086. doi:10.1007/s11056-019-09715-yHuang, L., Niu, G., Feagley, S. E., & Gu, M. (2019). Evaluation of a hardwood biochar and two composts mixes as replacements for a peat-based commercial substrate. Industrial Crops and Products, 129, 549-560. doi:10.1016/j.indcrop.2018.12.044Alvarez, J. M., Pasian, C., Lal, R., Lapez, R., & Ferna¡ndez, M. (2017). Vermicompost and biochar as substitutes of growing media in ornamental-plant production. Journal of Applied Horticulture, 19(03), 205-214. doi:10.37855/jah.2017.v19i03.37Steiner, C., Das, K. C., Melear, N., & Lakly, D. (2010). Reducing Nitrogen Loss during Poultry Litter Composting Using Biochar. Journal of Environmental Quality, 39(4), 1236-1242. doi:10.2134/jeq2009.0337Wang, C., Lu, H., Dong, D., Deng, H., Strong, P. J., Wang, H., & Wu, W. (2013). Insight into the Effects of Biochar on Manure Composting: Evidence Supporting the Relationship between N2O Emission and Denitrifying Community. Environmental Science & Technology, 47(13), 7341-7349. doi:10.1021/es305293hWang, Y., Villamil, M. B., Davidson, P. C., & Akdeniz, N. (2019). A quantitative understanding of the role of co-composted biochar in plant growth using meta-analysis. Science of The Total Environment, 685, 741-752. doi:10.1016/j.scitotenv.2019.06.244Sánchez-García, M., Alburquerque, J. A., Sánchez-Monedero, M. A., Roig, A., & Cayuela, M. L. (2015). Biochar accelerates organic matter degradation and enhances N mineralisation during composting of poultry manure without a relevant impact on gas emissions. Bioresource Technology, 192, 272-279. doi:10.1016/j.biortech.2015.05.003Maroušek, J., Hašková, S., Zeman, R., Žák, J., Vaníčková, R., Maroušková, A., … Myšková, K. (2015). Polemics on Ethical Aspects in the Compost Business. Science and Engineering Ethics, 22(2), 581-590. doi:10.1007/s11948-015-9664-yAbad, M., Fornes, F., Carrión, C., Noguera, V., Noguera, P., Maquieira, A., & Puchades, R. (2005). Physical Properties of Various Coconut Coir Dusts Compared to Peat. HortScience, 40(7), 2138-2144. doi:10.21273/hortsci.40.7.2138Laird, D., Fleming, P., Wang, B., Horton, R., & Karlen, D. (2010). Biochar impact on nutrient leaching from a Midwestern agricultural soil. Geoderma, 158(3-4), 436-442. doi:10.1016/j.geoderma.2010.05.012Jaiswal, A. K., Elad, Y., Paudel, I., Graber, E. R., Cytryn, E., & Frenkel, O. (2017). Linking the Belowground Microbial Composition, Diversity and Activity to Soilborne Disease Suppression and Growth Promotion of Tomato Amended with Biochar. Scientific Reports, 7(1). doi:10.1038/srep44382Elad, Y., David, D. R., Harel, Y. M., Borenshtein, M., Kalifa, H. B., Silber, A., & Graber, E. R. (2010). Induction of Systemic Resistance in Plants by Biochar, a Soil-Applied Carbon Sequestering Agent. Phytopathology®, 100(9), 913-921. doi:10.1094/phyto-100-9-0913Graber, E. R., Meller Harel, Y., Kolton, M., Cytryn, E., Silber, A., Rav David, D., … Elad, Y. (2010). Biochar impact on development and productivity of pepper and tomato grown in fertigated soilless media. Plant and Soil, 337(1-2), 481-496. doi:10.1007/s11104-010-0544-6Fornes, F., Belda, R. M., & Lidón, A. (2015). Analysis of two biochars and one hydrochar from different feedstock: focus set on environmental, nutritional and horticultural considerations. Journal of Cleaner Production, 86, 40-48. doi:10.1016/j.jclepro.2014.08.057Fornes, F., Belda, R. M., Carrión, C., Noguera, V., García-Agustín, P., & Abad, M. (2007). Pre-conditioning ornamental plants to drought by means of saline water irrigation as related to salinity tolerance. Scientia Horticulturae, 113(1), 52-59. doi:10.1016/j.scienta.2007.01.008Moran, R. (1982). Formulae for Determination of Chlorophyllous Pigments Extracted with N,N-Dimethylformamide. Plant Physiology, 69(6), 1376-1381. doi:10.1104/pp.69.6.1376Mendoza-Hernández, D., Fornes, F., & Belda, R. M. (2014). Compost and vermicompost of horticultural waste as substrates for cutting rooting and growth of rosemary. Scientia Horticulturae, 178, 192-202. doi:10.1016/j.scienta.2014.08.024Fornes, F., Mendoza-Hernandez, D., & Belda, R. M. (2013). Compost versus vermicompost as substrate constituents for rooting shrub cuttings. Spanish Journal of Agricultural Research, 11(2), 518. doi:10.5424/sjar/2013112-3304Esteban, R., Ariz, I., Cruz, C., & Moran, J. F. (2016). Review: Mechanisms of ammonium toxicity and the quest for tolerance. Plant Science, 248, 92-101. doi:10.1016/j.plantsci.2016.04.008Domínguez-Valdivia, M. D., Aparicio-Tejo, P. M., Lamsfus, C., Cruz, C., Martins-Loução, M. A., & Moran, J. F. (2008). Nitrogen nutrition and antioxidant metabolism in ammonium-tolerant and -sensitive plants. Physiologia Plantarum, 132(3), 359-369. doi:10.1111/j.1399-3054.2007.01022.xBritto, D. T., & Kronzucker, H. J. (2002). NH4+ toxicity in higher plants: a critical review. Journal of Plant Physiology, 159(6), 567-584. doi:10.1078/0176-1617-0774Fornes, F., Carrión, C., García-de-la-Fuente, R., Puchades, R., & Abad, M. (2010). Leaching composted lignocellulosic wastes to prepare container media: Feasibility and environmental concerns. Journal of Environmental Management, 91(8), 1747-1755. doi:10.1016/j.jenvman.2010.03.01

Rag1 immunodeficiency‐induced early aging and senescence in zebrafish are dependent on chronic inflammation and oxidative stress [Poster]

12th European Zebrafish Meeting, Krakow, 9-13 July 2023In mammals, recombination activating gene 1 (RAG1) plays a crucial role in adaptive immunity, generating a vast range of immunoglobulins. Rag1−/− zebrafish (Danio rerio) are viable and reach adulthood without obvious signs of infectious disease in standard nonsterile conditions, suggesting that innate immunity could be enhanced to compensate for the lack of adaptive immunity. By using microarray analysis, we confirmed that the expression of immunity- and apoptosis-related genes was increased in the rag1−/− fish. This tool also allows us to notice alterations of the DNA repair and cell cycle mechanisms in rag1−/− zebrafish. Several senescence and aging markers were analyzed. In addition to the lower lifespan of rag1−/− zebrafish compared to their wild-type (wt) siblings, rag1−/− showed a higher incidence of cell cycle arrest and apoptosis, a greater amount of phosphorylated histone H2AX, oxidative stress and decline of the antioxidant mechanisms, an upregulated expression and activity of senescence-related genes and senescence-associated β-galactosidase, respectively, diminished telomere length, and abnormal self-renewal and repair capacities in the retina and liver. Metabolomic analysis also demonstrated clear differences between wt and rag1−/− fish, as was the deficiency of the antioxidant metabolite L-acetylcarnitine (ALCAR) in rag1−/− fish. Therefore, Rag1 activity does not seem to be limited to V(D)J recombination but is also involved in senescence and aging. Furthermore, we confirmed the senolytic effect of ABT-263, a known senolytic compound and, for the first time, the potential in vivo senolytic activity of the antioxidant agent ALCAR, suggesting that this metabolite is essential to avoid premature agingN

CIBERER : Spanish national network for research on rare diseases: A highly productive collaborative initiative

Altres ajuts: Instituto de Salud Carlos III (ISCIII); Ministerio de Ciencia e Innovación.CIBER (Center for Biomedical Network Research; Centro de Investigación Biomédica En Red) is a public national consortium created in 2006 under the umbrella of the Spanish National Institute of Health Carlos III (ISCIII). This innovative research structure comprises 11 different specific areas dedicated to the main public health priorities in the National Health System. CIBERER, the thematic area of CIBER focused on rare diseases (RDs) currently consists of 75 research groups belonging to universities, research centers, and hospitals of the entire country. CIBERER's mission is to be a center prioritizing and favoring collaboration and cooperation between biomedical and clinical research groups, with special emphasis on the aspects of genetic, molecular, biochemical, and cellular research of RDs. This research is the basis for providing new tools for the diagnosis and therapy of low-prevalence diseases, in line with the International Rare Diseases Research Consortium (IRDiRC) objectives, thus favoring translational research between the scientific environment of the laboratory and the clinical setting of health centers. In this article, we intend to review CIBERER's 15-year journey and summarize the main results obtained in terms of internationalization, scientific production, contributions toward the discovery of new therapies and novel genes associated to diseases, cooperation with patients' associations and many other topics related to RD research

Caracteriación de Car D, un factor transcripcional de la bacteria Myxococcus xanthus / Mª Luisa Cayuela Fuentes ; director Francisco J. Murillo Araujo.

Tesis-Universidad de Murcia.Consulte la tesis en: BCA. GENERAL. ARCHIVO UNIVERSITARIO. D 583.Consulte la tesis en: BCA. GENERAL. ARCHIVO UNIVERSITARIO. T.M.-1834

Senescence-Independent Anti-Inflammatory Activity of the Senolytic Drugs Dasatinib, Navitoclax, and Venetoclax in Zebrafish Models of Chronic Inflammation

Telomere shortening is the main molecular mechanism of aging, but not the only one. The adaptive immune system also ages, and older organisms tend to develop a chronic pro-inflammatory status with low-grade inflammation characterized by chronic activation of the innate immune system, called inflammaging. One of the main stimuli that fuels inflammaging is a high nutrient intake, triggering a metabolic inflammation process called metainflammation. In this study, we report the anti-inflammatory activity of several senolytic drugs in the context of chronic inflammation, by using two different zebrafish models: (i) a chronic skin inflammation model with a hypomorphic mutation in spint1a, the gene encoding the serine protease inhibitor, kunitz-type, 1a (also known as hai1a) and (ii) a non-alcoholic fatty liver disease/non-alcoholic steatohepatitis (NAFLD/NASH) model with inflammation induced by a high-fat diet. Our results show that, although these models do not manifest premature aging, the senolytic drugs dasatinib, navitoclax, and venetoclax have an anti-inflammatory effect that results in the amelioration of chronic inflammation