Научно-технический семинар «Повышение эксплуатационной надежности линейной части магистральных газопроводов газотранспортной системы Украины»

С 11 по 15 марта 2002 г. Научно-технический комплекс «Институт электросварки им. II. О. Па-топа» Национальной Академии наук Украины совместно с ДК «Укртрансгаз» провели научно-технический семинар «Повышение эксплуатационной надежности линейной части магистральных газопроводов газотранспортной системы Украины». Семинар прошел в рамках Проекта Программы развития ООН «Обмен технологической информацией в Украине для поддержки экономических реформ»

Marchaux – Rue de la Comtesse

Une demande de permis de construire sur les parcelles AC19 et AC20 (AC72p) de la commune de Marchaux, au nord-est de Besançon, est à l’origine de ce diagnostic, dans un environnement archéologique attesté. Le terrain, d’une surface de quelque 4 500 m2 légèrement déclive, occupe une éminence calcaire sur le versant nord du village. Les cinq sondages réalisés (680 m2 au total) ont permis de localiser huit sépultures regroupées sur le sommet de la pente. Orientées nord-sud, ces dernières sont al..

Brainomics: A management system for exploring and merging heterogeneous brain mapping data

International audienceWe propose an open source solution to manage brain imaging datasets and associated meta data. This framework is a powerful querying and reporting tool, customized for the needs of the emerging imaging-genetics field. A demonstration website and more details are available at http:/brainomics.cea.fr

The THAP–zinc finger protein THAP1 regulates endothelial cell proliferation through modulation of pRB/E2F cell-cycle target genes

AbstractWe recently cloned a novel human nuclear factor (designated THAP1) from postcapillary venule endothelial cells (ECs) that contains a DNA-binding THAP domain, shared with zebrafish E2F6 and several Caenorhabditis elegans proteins interacting genetically with retinoblastoma gene product (pRB). Here, we show that THAP1 is a physiologic regulator of EC proliferation and cell-cycle progression, 2 essential processes for angiogenesis. Retroviral-mediated gene transfer of THAP1 into primary human ECs inhibited proliferation, and large-scale expression profiling with microarrays revealed that THAP1-mediated growth inhibition is due to coordinated repression of pRB/E2F cell-cycle target genes. Silencing of endogenous THAP1 through RNA interference similarly inhibited EC proliferation and G1/S cell-cycle progression, and resulted in down-regulation of several pRB/E2F cell-cycle target genes, including RRM1, a gene required for S-phase DNA synthesis. Chromatin immunoprecipitation assays in proliferating ECs showed that endogenous THAP1 associates in vivo with a consensus THAP1-binding site found in the RRM1 promoter, indicating that RRM1 is a direct transcriptional target of THAP1. The similar phenotypes observed after THAP1 overexpression and silencing suggest that an optimal range of THAP1 expression is essential for EC proliferation. Together, these data provide the first links in mammals among THAP proteins, cell proliferation, and pRB/E2F cell-cycle pathways

Alternative methods to evaluate the protective ability of sunscreen against photo-genotoxicity

Numerous epidemiological investigations show that sunlight is carcinogenic to humans and that the use of sunscreen may be effective in decreasing the risk of skin cancer. The biological activity of a sunscreen is evaluated by its ability to protect human skin from erythema as represented by a Sun Protection Factor (SPF). We propose that the sunscreen's protective effect against sunlight-induced genotoxicity, including mutation, should also be taken into account. In this study we examined the protective ability of sunscreens against natural sunlight and UV-induced genotoxicity in Drosophila somatic cells. We prepared three kinds of sunscreen samples, each with an SPF value of 20, 40 or 60 and compared their protective activities with commercial sunscreens. When a sunscreen of SPF 20, 40 or 60 was pasted on the plastic cover of a petri dish in which Drosophila larvae were exposed to the sun or UV lamps, genotoxicity decreased as the SPF of the sunscreen increased, relative to levels of genotoxicity observed in samples without sunscreen. However, the protective abilities of sunscreens were unexpectedly not so different from each other. To reveal the relationship between the protective activity of sunscreen and the wavelength of light with which larvae were irradiated through the sunscreen, we measured the transmittance of light through the petri dish cover on which the sunscreen was pasted. Effective protection was demonstrated by removing components of light whose wavelengths were below 315 nm. We suggest, that the measurement of anti-genotoxic activity and the determination of the wavelengths of light transmitted through the sunscreen should be an alternative method for evaluating the effectiveness of a sunscreen.</p

Lempdes – Les Gibaudonnes et La Ponsole, Fontanille, Croix Saint-Masson (ZAC de la Fontanille II)

L’emprise de la future ZAC de la Fontanille à Lempdes est située entre les ronds-points de la Croix Saint-Masson et de Chazal. Les parcelles concernées par l’opération archéologique se trouvent au pied de la butte marnocalcaire de Chambussière, en bordure sud de la grande plaine de La Limagne, dans le secteur communément appelée les Marais. Cette opération, menée du 13 novembre 2006 au 23 décembre 2006 et du 8 au 26 janvier 2007, concerne un projet d’une surface de 375 776 m2 (BSR 2006, p. 10..

Structural determinants of specific DNA-recognition by the THAP zinc finger

Human THAP1 is the prototype of a large family of cellular factors sharing an original THAP zinc-finger motif responsible for DNA binding. Human THAP1 regulates endothelial cell proliferation and G1/S cell-cycle progression, through modulation of pRb/E2F cell-cycle target genes including rrm1. Recently, mutations in THAP1 have been found to cause DYT6 primary torsion dystonia, a human neurological disease. We report here the first 3D structure of the complex formed by the DNA-binding domain of THAP1 and its specific DNA target (THABS) found within the rrm1 target gene. The THAP zinc finger uses its double-stranded β-sheet to fill the DNA major groove and provides a unique combination of contacts from the β-sheet, the N-terminal tail and surrounding loops toward the five invariant base pairs of the THABS sequence. Our studies reveal unprecedented insights into the specific DNA recognition mechanisms within this large family of proteins controlling cell proliferation, cell cycle and pluripotency

Pathogenic Bacteria Target NEDD8-Conjugated Cullins to Hijack Host-Cell Signaling Pathways

The cycle inhibiting factors (Cif), produced by pathogenic bacteria isolated from vertebrates and invertebrates, belong to a family of molecules called cyclomodulins that interfere with the eukaryotic cell cycle. Cif blocks the cell cycle at both the G1/S and G2/M transitions by inducing the stabilization of cyclin-dependent kinase inhibitors p21waf1 and p27kip1. Using yeast two-hybrid screens, we identified the ubiquitin-like protein NEDD8 as a target of Cif. Cif co-compartmentalized with NEDD8 in the host cell nucleus and induced accumulation of NEDD8-conjugated cullins. This accumulation occurred early after cell infection and correlated with that of p21 and p27. Co-immunoprecipitation revealed that Cif interacted with cullin-RING ubiquitin ligase complexes (CRLs) through binding with the neddylated forms of cullins 1, 2, 3, 4A and 4B subunits of CRL. Using an in vitro ubiquitylation assay, we demonstrate that Cif directly inhibits the neddylated CUL1-associated ubiquitin ligase activity. Consistent with this inhibition and the interaction of Cif with several neddylated cullins, we further observed that Cif modulates the cellular half-lives of various CRL targets, which might contribute to the pathogenic potential of diverse bacteria

Jean Cayrol, Lazzaro tra di noi. seguito da Testimonianza e letteratura. Traduzione di Marina Galletti

Si tratta della prima traduzione italiana di "Lazare parmi nous" e di "Témoignage et littérature" di Jean Cayrol