10 research outputs found
Relationship of white and gray matter abnormalities to clinical and genetic features in myotonic dystrophy type 1
AbstractBackgroundMyotonic dystrophy type 1 (DM1) represents a multisystemic disorder in which diffuse brain white and gray matter alterations related to clinical and genetic features have been described. We aimed to evaluate in the brain of adult patients with DM1 (i) white and gray matter differences, including cortical-subcortical gray matter volume and cortical thickness and (ii) their correlation with clinical disability, global neuropsychological performance and triplet expansion.MethodsWe included 24 adult genetically-confirmed DM1 patients (14 males; age: 38.5±11.8years) and 25 age- and sex-matched healthy controls (14 males; age: 38.5±11.3years) who underwent an identical brain MR protocol including high-resolution 3D T1-weighted, axial T2 FLAIR and DTI sequences. All patients underwent an extensive clinical and neuropsychological evaluation. Voxel-wise analyses of white matter, performed by using Tract Based Spatial Statistics, and of gray matter, with Voxel-based Morphometry and Cortical Thickness, were carried out in order to test for differences between patients with DM1 and healthy controls (p<0.05, corrected). The correlation between MRI measures and clinical-genetic features was also assessed.ResultsPatients with DM1 showed widespread abnormalities of all DTI parameters in the white matter, which were associated with reduced gray matter volume in all brain lobes and thinning in parieto-temporo-occipital cortices, albeit with less extensive cortical alterations when congenital cases were removed from the analyses. White matter alterations correlated with clinical disability, global cognitive performance and triplet expansions.ConclusionIn patients with DM1, the combined smaller overall gray matter volume and white matter alterations seem to be the main morpho-structural substrates of CNS involvement in this condition. The correlation of white matter differences with both clinical and genetic findings lends support to this notion
Functional and expression study uncovered hepatocyte plasma membrane ecto-ATP synthase as a novel player in liver regeneration
ATP synthase, canonically mitochondrially located, is reported
to be ectopically expressed on the plasma membrane outer
face of several cell types. We analysed, for the first time, the
expression and catalytic activities of the ecto- and mitochondrial
ATP synthase during liver regeneration. Liver regeneration was
induced in rats by two-thirds partial hepatectomy. The protein
level and the ATP synthase and/or hydrolase activities of the
hepatocyte ecto- and mitochondrial ATP synthase were analysed
on freshly isolated hepatocytes and mitochondria from control,
sham-operated and partial hepatectomized rats. During the
priming phase of liver regeneration, 3 h after partial hepatectomy,
livermitochondria showed amarked lowering of theATP synthase
protein level that was reflected in the impairment of both
ATP synthesis and hydrolysis. The ecto-ATP synthase level, in
3 h partial hepatectomized hepatocytes, was decreased similarly
to the level of the mitochondrial ATP synthase, associated
with a lowering of the ecto-ATP hydrolase activity coupled
to proton influx. Noteworthily, the ecto-ATP synthase activity
coupled to proton efflux was completely inhibited in 3 h partial
hepatectomized hepatocytes, even in the presence of a marked
intracellular acidification that would sustain it as in control and
sham-operated hepatocytes. At the end of the liver regeneration,
7 days after partial hepatectomy, the level and the catalytic
activities of the ecto- and mitochondrial ATP synthase reached
the control and sham-operated values. The specific modulation
of hepatocyte ecto-ATP synthase catalytic activities during liver
regeneration priming phase may modulate the extracellular
ADP/ATP levels and/or proton influx/efflux trafficking, making
hepatocyte ecto-ATP synthase a candidate for a novel player in
the liver regeneration process
Mitochondrial dysfunction in myotonic dystrophy type 1
The pathophysiological mechanism linking the nucleotide expansion in the DMPK gene to the clinical manifestations of myotonic dystrophy type 1 (DM1) is still unclear. In vitro studies demonstrate DMPK involvement in the redox homeostasis of cells and the mitochondrial dysfunction in DM1, but in vivo investigations of oxidative metabolism in skeletal muscle have provided ambiguous results and have never been performed in the brain. Twenty-five DM1 patients (14M, 39\u2009\ub1\u200911years) underwent brain proton MR spectroscopy (1H-MRS), and sixteen cases (9M, 40 \ub1 13 years old) also calf muscle phosphorus MRS (31P-MRS). Findings were compared to those of sex- and age-matched controls. Eight DM1 patients showed pathological increase of brain lactate and, compared to those without, had larger lateral ventricles (p\u2009<\u20090.01), smaller gray matter volumes (p\u2009<\u20090.05) and higher white matter lesion load (p\u2009<\u20090.05). A reduction of phosphocreatine/inorganic phosphate (p\u2009<\u20090.001) at rest and, at first minute of exercise, a lower [phosphocreatine] (p\u2009=\u20090.003) and greater [ADP] (p\u2009=\u20090.004) were found in DM1 patients compared to controls. The post-exercise indices of muscle oxidative metabolism were all impaired in DM1, including the increase of time constant of phosphocreatine resynthesis (TC PCr, p\u2009=\u20090.038) and the reduction of the maximum rate of mitochondrial ATP synthesis (p\u2009=\u20090.033). TC PCr values correlated with the myotonic area score (\u3c1\u2009=\u20090.74, p\u2009=\u20090.01) indicating higher impairment of muscle oxidative metabolism in clinically more affected patients. Our findings provide clear in vivo evidence of multisystem impairment of oxidative metabolism in DM1 patients, providing a rationale for targeted treatment enhancing energy metabolism
Impairment of brain and muscle energy metabolism detected by magnetic resonance spectroscopy in hereditary spastic paraparesis type 28 patients with DDHD1 mutations
Function and expression study uncovered hepatocyte plasma membrane ecto-ATP synthase as a novel player in liver regeneration
Transient receptor potential vanilloid subtype 1 depletion mediates mechanical allodynia through cellular signal alterations in small-fiber neuropathy
A survey of clinical features of allergic rhinitis in adults
Background: Allergic rhinitis (AR) has high prevalence and substantial socio-economic burden.Material/Methods: The study included 35 Italian Centers recruiting an overall number of 3383 adult patients with rhinitis (48% males, 52% females, mean age 29.1, range 18-45 years). For each patient, the attending physician had to fill in a standardized questionnaire, covering, in particular, some issues such as the ARIA classification of allergic rhinitis (AR), the results of skin prick test (SPT), the kind of treatment, the response to treatment, and the satisfaction with treatment.Results: Out of the 3383 patients with rhinitis, 2788 (82.4%) had AR: 311 (11.5%) had a mild intermittent, 229 (8.8%) a mild persistent, 636 (23.5%) a moderate-severe intermittent, and 1518 (56.1%) a moderate-severe persistent form. The most frequently used drugs were oral antihistamines (77.1%) and topical corticosteroids (60.8%). The response to treatment was judged as excellent in 12.2%, good in 41.3%, fair in 31.2%, poor in 14.5%, and very bad in 0.8% of subjects. The rate of treatment dissatisfaction was significantly higher in patients with moderate-to-severe AR than in patients with mild AR (p<0.0001). Indication to allergen immunotherapy (AIT) was significantly more frequent (p<0.01) in patients with severe AR than with mild AR.Conclusions: These findings confirm the appropriateness of ARIA guidelines in classifying the AR patients and the association of severe symptoms with unsuccessful drug treatment. The optimal targeting of patients to be treated with AIT needs to be reassessed
Ezetimibe added to statin therapy after acute coronary syndromes
BACKGROUND: Statin therapy reduces low-density lipoprotein (LDL) cholesterol levels and the risk of cardiovascular events, but whether the addition of ezetimibe, a nonstatin drug that reduces intestinal cholesterol absorption, can reduce the rate of cardiovascular events further is not known. METHODS: We conducted a double-blind, randomized trial involving 18,144 patients who had been hospitalized for an acute coronary syndrome within the preceding 10 days and had LDL cholesterol levels of 50 to 100 mg per deciliter (1.3 to 2.6 mmol per liter) if they were receiving lipid-lowering therapy or 50 to 125 mg per deciliter (1.3 to 3.2 mmol per liter) if they were not receiving lipid-lowering therapy. The combination of simvastatin (40 mg) and ezetimibe (10 mg) (simvastatin-ezetimibe) was compared with simvastatin (40 mg) and placebo (simvastatin monotherapy). The primary end point was a composite of cardiovascular death, nonfatal myocardial infarction, unstable angina requiring rehospitalization, coronary revascularization ( 6530 days after randomization), or nonfatal stroke. The median follow-up was 6 years. RESULTS: The median time-weighted average LDL cholesterol level during the study was 53.7 mg per deciliter (1.4 mmol per liter) in the simvastatin-ezetimibe group, as compared with 69.5 mg per deciliter (1.8 mmol per liter) in the simvastatin-monotherapy group (P<0.001). The Kaplan-Meier event rate for the primary end point at 7 years was 32.7% in the simvastatin-ezetimibe group, as compared with 34.7% in the simvastatin-monotherapy group (absolute risk difference, 2.0 percentage points; hazard ratio, 0.936; 95% confidence interval, 0.89 to 0.99; P = 0.016). Rates of pre-specified muscle, gallbladder, and hepatic adverse effects and cancer were similar in the two groups. CONCLUSIONS: When added to statin therapy, ezetimibe resulted in incremental lowering of LDL cholesterol levels and improved cardiovascular outcomes. Moreover, lowering LDL cholesterol to levels below previous targets provided additional benefit