Analysis of antenal sensilla patterns of Rhodnius prolixus from Colombia and Venezuela

Antennal sensilla patterns were used to analyze population variation of domestic Rhodnius prolixus from six departments and states representing three biogeographical regions of Colombia and Venezuela. Discriminant analysis of the patterns of mechanoreceptors and of three types of chemoreceptors on the pedicel and flagellar segments showed clear differentiation between R. prolixus populations east and west of the Andean Cordillera. The distribution of thick and thin-walled trichoids on the second flagellar segment also showed correlation with latitude, but this was not seen in the patterns of other sensilla. The results of the sensilla patterns appear to be reflecting biogeographic features or population isolation rather than characters associated with different habitats and lend support to the idea that domestic R. prolixus originated in the eastern region of the Andes.Fil: Esteban, Lyda. Universidad Industrial de Santander; ColombiaFil: Angulo, Víctor Manuel. Universidad Industrial de Santander; ColombiaFil: Dora Feliciangeli, M.. Universidad de Carabobo; VenezuelaFil: Catala, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja. - Universidad Nacional de La Rioja. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja. - Universidad Nacional de Catamarca. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja. - Secretaría de Industria y Minería. Servicio Geológico Minero Argentino. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja. - Provincia de La Rioja. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja; Argentin

Experimental evaluation of insecticidal paints against Triatoma infestans (Hemiptera: Reduviidae), under natural climatic conditions

Triatoma infestans is the main vector of Chagas disease in the Gran Chaco region of South America. The traditional spraying technique used for the application of pyrethroid insecticides has shown low efficiency in the elimination of the vector species populations occupying peridomestic structures of rural houses in the endemic area of Argentina. As part of studies looking for better alternatives, we evaluated the residual effect of insecticidal paints on the mortality of fourth instar nymphs of T. infestans. Results. The study was based on an experimental design that included two groups treated with an organophosphate (Inesfly 5A IGR) and a pyrethroid (Inesfly 5A IGR NG) formulations of the paint, that were applied on wood, cement blocks and adobe bricks under natural climatic conditions. A third group was an untreated control. Both paint formulations showed very long residual activity, producing mortality of 84% and 98% (pyrethroid and organophosphate formulations, respectively) after 12 months of the paint application. After eight months, nymphs exposed during 6 hours to the painted surfaces with the pyrethroid and organophosphate formulations showed 81.33% and 100% mortality, respectively. Conclusion. The organophosphate- and pyrethroid-based insecticidal paints showed a very long residual activity on the mortality of fourth instar nymphs of T infestans, compared with the traditional spraying technique used for the application of pyrethroid insecticides in peridomestic structures of rural houses in the endemic region for Chagas disease in the Gran Chaco of Argentina. The application of the paints by trained personnel of the vector control programmes could be considered as an alternative control tool in areas where the traditional methods have failed or showed low efficacy.Fil: Amelotti, Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja. - Universidad Nacional de La Rioja. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja. - Universidad Nacional de Catamarca. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja. - Secretaría de Industria y Minería. Servicio Geológico Minero Argentino. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja. - Provincia de La Rioja. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja; ArgentinaFil: Catala, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja. - Universidad Nacional de La Rioja. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja. - Universidad Nacional de Catamarca. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja. - Secretaría de Industria y Minería. Servicio Geológico Minero Argentino. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja. - Provincia de La Rioja. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja; ArgentinaFil: Gorla, David Eladio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja. - Universidad Nacional de La Rioja. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja. - Universidad Nacional de Catamarca. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja. - Secretaría de Industria y Minería. Servicio Geológico Minero Argentino. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja. - Provincia de La Rioja. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de La Rioja; Argentin

The use of the invasive allien species Rugulopteryx okamurae (Ochrophyta) as source of cosmeceutical compounds

The brown macroalga Rugulopteryx okamurae (Dictyotales, Ochrophyta) is invading the western coast Mediterranean sea from 2016. In Spain, it is consider exotic invasive species from December, 2020. Recently , it is starting to invade areas in Provence coast (France) and the Azores Islands (Portugal). This risk studies have stablished that it can potentially extend in the whole Mediterranean Sea. This algal species originated from East Asia was identified the first time in the Mediterranean Sea by 2002 in the Thau Lagoon (France) , where its entering was associated to oyster aquaculture. Rugulopteryx can be fixed in hard substrates from the surface to 40 m depth presenting the highest cover (95-100%) about 10-20 m depth. It can be also mantained long time as free floating algae and in addition it can be observed as beach cast algae. Drastic alterations in the biodiversity of the native communities and high impacts in fish and touristic economical sectors are being produced . In addition to the prevention, one strategy to control the invasion in high impacted areas is to harvest specimens for commercial use to obtained natural bioactive compounds . This strategy presents a two-folded opportunity i.e high availability of biological material for the extraction of bioactive compounds for cosmeceutical uses and through specimen collection , mitigating negative effects caused by alien species, contributing to ecosystem integrity and sustainability. In this study seasonal pattern of biomass productivity, photosynthetic capacity and the accumulation of high cosmeceutical compounds as polyphenols and fucoidans and biochemical content (protein, lipids and carbohydrates) are presented. R.okamurae has a high carbon content (35-42%) and broad range of internal N content (1.5-4.5%).Photosynthetic capacity is maintained very high throughout the year with the maximal production and accumulation of high value compounds in summer. Toxicity of extracts was also evaluated.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Determination of organothiophosphorus pesticides in water by liquid chromatography and post-column chemiluminescence with cerium(IV)

A new, fast, selective and sensitive method has been developed for the simultaneous determination of nine organothiophosphorus (OTP) pesticides, namely omethoate, dimethoate, disulfoton-sulfoxide, methidathion, phosmet, malathion, diazinon, pirimiphos-methyl and chlorpyrifos. The pesticides were separated on a Kinetex C18 column by gradient elution with acetonitrile:water. A post-column basic hydrolysis of the pesticides and later a chemiluminescence (CL) reaction with cerium (IV) in acid medium was carried out. Hexadecylpyridinium chloride highly enhanced the CL emission. Under optimized conditions, linearity, precision, limits of detection and quantification, and accuracy were determined. Both selectivity and sensitivity were compared with those obtained with UV detection. In combination with SPE, limits of detection in the range 15-80 ng/L and 5-30 ng/L were obtained when 250 mL and 1000 mL of solution were treated, respectively. When applied to 250 mL of sample the inter-day precision of the method was between 3.5% and 7.3% and the intra-day precision between 2.9% and 6.0%. The method was applied to determine OTP pesticides in spiked water samples from different origins: irrigation, river, sea, ground, spring, mineral and tap waters, being the percentage of recovery of added amounts near 100% form most of the pesticides.Catalá Icardo, M.; Lahuerta Zamora, L.; Torres-Cartas, S.; Meseguer-Lloret, S. (2014). Determination of organothiophosphorus pesticides in water by liquid chromatography and post-column chemiluminescence with cerium(IV). Journal of Chromatography A. 1341:31-40. doi:10.1016/j.chroma.2014.03.0243140134

Selective and sensitive chemiluminescence determination of MCPB: flow injection and liquid chromatography

This paper was published in Applied Spectroscopy and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://dx.doi.org/10.1177/0003702815620133 . Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.Two new chemiluminescence (CL) methods are described for the determination of the herbicide 4-(4-chloro-o-tolyloxy) butyric acid (MCPB). First, a flow injection chemiluminescence (FI-CL) method is proposed. In this method, MCPB is photodegraded with an ultraviolet (UV) lamp and the photoproducts formed provide a great CL signal when they react with ferricyanide in basic medium. Second, a high-performance liquid chromatography chemiluminescence (HPLC-CL) method is proposed. In this method, before the photodegradation and CL reaction, the MCPB and other phenoxyacid herbicides are separated in a C18 column. The experimental conditions for the FI-CL and HPLC-CL methods are optimized. Both methods present good sensitivity, the detection limits being 0.12 mg L 1 and 0.1 mg L 1 (for FI-CL and HPLC-CL, respectively) when solid phase extraction (SPE) is applied. Intra- and interday relative standard deviations are below 9.9%. The methods have been satisfactorily applied to the analysis of natural water samples. FI-CL method can be employed for the determination of MCPB in simple water samples and for the screening of complex water samples in a fast, economic, and simple way. The HPLC-CL method is more selective, and allows samples that have not been resolved with the FI-CL method to be solved.Meseguer-Lloret, S.; Torres-Cartas, S.; Catalá-Icardo, M.; Gómez Benito, C. (2016). Selective and sensitive chemiluminescence determination of MCPB: flow injection and liquid chromatography. Applied Spectroscopy. 70(2):312-321. doi:10.1177/0003702815620133S312321702Moral, A., Caballo, C., Sicilia, M. D., & Rubio, S. (2012). Highly efficient microextraction of chlorophenoxy acid herbicides in natural waters using a decanoic acid-based nanostructured solvent prior to their quantitation by liquid chromatography–mass spectrometry. Analytica Chimica Acta, 709, 59-65. doi:10.1016/j.aca.2011.10.016Herrero-Hernández, E., Rodríguez-Gonzalo, E., Andrades, M. S., Sánchez-González, S., & Carabias-Martínez, R. (2013). Occurrence of phenols and phenoxyacid herbicides in environmental waters using an imprinted polymer as a selective sorbent. Science of The Total Environment, 454-455, 299-306. doi:10.1016/j.scitotenv.2013.03.029Baggiani, C., Giovannoli, C., Anfossi, L., & Tozzi, C. (2001). Molecularly imprinted solid-phase extraction sorbent for the clean-up of chlorinated phenoxyacids from aqueous samples. Journal of Chromatography A, 938(1-2), 35-44. doi:10.1016/s0021-9673(01)01126-8Wintersteiger, R., Goger, B., & Krautgartner, H. (1999). Quantitation of chlorophenoxy acid herbicides by high-performance liquid chromatography with coulometric detection. Journal of Chromatography A, 846(1-2), 349-357. doi:10.1016/s0021-9673(99)00429-xPeruzzi, M., Bartolucci, G., & Cioni, F. (2000). Determination of phenoxyalkanoic acids and other herbicides at the ng/ml level in water by solid-phase extraction with poly(divinylbenzene-co-N-vinylpyrrolidone) sorbent and high-performance liquid chromatography–diode-array detection. Journal of Chromatography A, 867(1-2), 169-175. doi:10.1016/s0021-9673(99)01141-3Ranz, A., & Lankmayr, E. (2006). Screening and optimization of the derivatization of polar herbicides with trimethylanilinium hydroxide for GC-MS analysis. Journal of Biochemical and Biophysical Methods, 69(1-2), 3-14. doi:10.1016/j.jbbm.2006.02.007Nuhu, A. A., Basheer, C., Alhooshani, K., & Al-Arfaj, A. R. (2012). Determination of phenoxy herbicides in water samples using phase transfer microextraction with simultaneous derivatization followed by GC-MS analysis. Journal of Separation Science, 35(23), 3381-3388. doi:10.1002/jssc.201200218Jiménez, J. J. (2013). Simultaneous liquid–liquid extraction and dispersive solid-phase extraction as a sample preparation method to determine acidic contaminants in river water by gas chromatography/mass spectrometry. Talanta, 116, 678-687. doi:10.1016/j.talanta.2013.07.052EREMIN, S., LAASSIS, B., & AARON, J. (1996). Photochemical-fluorimetric method for the determination of total chlorophenoxyacid herbicides. Talanta, 43(3), 295-301. doi:10.1016/0039-9140(95)01751-8Jafari, M. T., Saraji, M., & Yousefi, S. (2012). Negative electrospray ionization ion mobility spectrometry combined with microextraction in packed syringe for direct analysis of phenoxyacid herbicides in environmental waters. Journal of Chromatography A, 1249, 41-47. doi:10.1016/j.chroma.2012.06.024Tsogas, G. Z., Giokas, D. L., Nikolakopoulos, P. G., Vlessidis, A. G., & Evmiridis, N. P. (2006). Determination of the pesticide carbaryl and its photodegradation kinetics in natural waters by flow injection–direct chemiluminescence detection. Analytica Chimica Acta, 573-574, 354-359. doi:10.1016/j.aca.2005.11.058Albert-García, J. R., & Calatayud, J. M. (2008). Determination of the herbicide benfuresate by its photo-induced chemiluminescence using flow multicommutation methodology. Talanta, 75(3), 717-724. doi:10.1016/j.talanta.2007.12.003Catalá-Icardo, M., López-Paz, J. L., Choves-Barón, C., & Peña-Bádena, A. (2012). Native vs photoinduced chemiluminescence in dimethoate determination. Analytica Chimica Acta, 710, 81-87. doi:10.1016/j.aca.2011.10.043Gómez-Benito, C., Meseguer-Lloret, S., & Torres-Cartas, S. (2013). Sensitive determination of Fenamiphos in water samples by flow injection photoinduced chemiluminescence. International Journal of Environmental Analytical Chemistry, 93(2), 152-165. doi:10.1080/03067319.2012.663755Beale, D. J., Porter, N. A., & Roddick, F. A. (2009). A fast screening method for the presence of atrazine and other triazines in water using flow injection with chemiluminescent detection. Talanta, 78(2), 342-347. doi:10.1016/j.talanta.2008.11.033Catalá-Icardo, M., López-Paz, J. L., & Pérez-Plancha, L. M. (2014). Fast Determination of Thiacloprid by Photoinduced Chemiluminescence. Applied Spectroscopy, 68(6), 642-648. doi:10.1366/13-07330Torres-Cartas, S., Gómez-Benito, C., & Meseguer-Lloret, S. (2011). FI on-line chemiluminescence reaction for determination of MCPA in water samples. Analytical and Bioanalytical Chemistry, 402(3), 1289-1296. doi:10.1007/s00216-011-5567-1Catalá-Icardo, M., Lahuerta-Zamora, L., Torres-Cartas, S., & Meseguer-Lloret, S. (2014). Determination of organothiophosphorus pesticides in water by liquid chromatography and post-column chemiluminescence with cerium(IV). Journal of Chromatography A, 1341, 31-40. doi:10.1016/j.chroma.2014.03.024Huertas-Pérez, J. F., & García-Campaña, A. M. (2008). Determination of N-methylcarbamate pesticides in water and vegetable samples by HPLC with post-column chemiluminescence detection using the luminol reaction. Analytica Chimica Acta, 630(2), 194-204. doi:10.1016/j.aca.2008.09.047Orejuela, E., & Silva, M. (2003). Monitoring some phenoxyl-type N-methylcarbamate pesticide residues in fruit juices using high-performance liquid chromatography with peroxyoxalate-chemiluminescence detection. Journal of Chromatography A, 1007(1-2), 197-201. doi:10.1016/s0021-9673(03)00934-8GALERA, M., GARCIA, M., & VALVERDE, R. (2008). Determination of photoirradiated high polar benzoylureas in tomato by HPLC with luminol chemiluminescence detection. Talanta, 76(4), 815-823. doi:10.1016/j.talanta.2008.04.052Rosales-Conrado, N., León-González, M. E., Pérez-Arribas, L. V., & Polo-Díez, L. M. (2005). Effect of temperature on the separation of chlorophenoxy acids and carbamates by capillary high-performance liquid chromatography and UV (or diode array) detection. Journal of Chromatography A, 1081(1), 114-121. doi:10.1016/j.chroma.2004.12.083Geerdink, R. B., van Tol-Wildenburg, S., Niessen, W. M. A., & Brinkman, U. A. T. (1997). Determination of Phenoxy Acid Herbicides From Aqueous Samples by Improved Clean-up on Polymeric Pre-columns at High pH. The Analyst, 122(9), 889-894. doi:10.1039/a702338

Use of Single Board Computers as Smart Sensors in the Manufacturing Industry

The continuously growing presence of cyber-physical systems in the industry, especially in the field of processes automation and control, represents the paradigm of the so called fourth industrial revolution, in which the systems are smarter, faster and more optimized by means of artificial intelligence, control systems and sensors networks. The presence of ICT and automation systems guarantees energy and other resources efficiency along the whole value chain of industrial processes. Especially important is the case of the smart sensors, in which a conventional sensor is equipped with interfacing methodologies for signal processing and decision making. In this article the capabilities of using a single board computer as a smart sensor are explored.Postprint (published version

Photografted fluoropolymers as novel chromatographic supports for polymeric monolithic stationary phases

[EN] In this study, porous polymer monoliths were in situ synthesized in fluoropolymers tubing to prepare microbore HPLC columns. To ensure the formation of robust homogeneous polymer monoliths in these housing supports, the inner surface of fluoropolymer tubing was modified in a two-step photografting process. Raman spectroscopy and scanning electron microscopy (SEM) confirmed the successful modification of the inner poly(ethylene-co-tetrafluoroethylene) (ETFE) wall and the subsequent attachment of a monolith onto the wall. Poly(glycidyl methacrylate-co-divinylbenzene), poly(butyl methacrylate-co-ethyleneglycol dimethacrylate) and poly(styrene-co-divinylbenzene) monoliths were in situ synthesized by thermal polymerization within the confines of surface vinylized ETFE tubes. The resulting monoliths exhibited good permeability and mechanical stability (pressure resistance up to 9¿MPa). The chromatographic performance of these different monolithic columns was evaluated via the separation of alkyl benzenes and proteins in a conventional HPLC system.This work was supported by project PROMETEO/2016/145 (Conselleria d'Educacio, Investigacio, Cultura i Esport,Esport, Generalitat Valenciana, Spain). The authors also thank Dr. S. Laredo-Ortiz from the Atomic Spectroscopy section of the SCSIE (University of Valencia), for her help in Raman measurements.Catalá-Icardo, M.; Torres-Cartas, S.; Meseguer-Lloret, S.; Simó-Alfonso, E.; Herrero Martínez, J. (2018). Photografted fluoropolymers as novel chromatographic supports for polymeric monolithic stationary phases. Talanta. 187:216-222. doi:10.1016/j.talanta.2018.05.026S21622218

Accelerated Growth Plate Mineralization and Foreshortened Proximal Limb Bones in Fetuin-A Knockout Mice

PMCID: PMC3473050This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Antioxidant Activity and Phenolic Content of Marine Dissolved Organic Matter and Their Relation to Molecular Composition

The potential of marine dissolved organic matter (DOM) for free radical scavenging has been extensively evaluated, however, the quantitative assessment of the antioxidant potential has been recently measured for the first time. The linkage of the DOM antioxidant potential to its molecular composition has not yet been examined. Following this line, this article takes a step forward by assessing, throughout a polarity-mediated fractionation, (1) the antioxidant capacity and phenolic content and (2) the molecular characterization of DOM in a more exhaustive manner. (3) The DOM antioxidant potential and phenolic content was linked to the molecular composition of DOM, which was molecularly characterized using ultrahigh resolution Fourier transform Ion Cyclotron Resonance mass spectrometry (FT-ICR MS). Antioxidant activity and phenolic content were quantified by the free radical 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS⋅) and the Folin-Ciocalteu methods, respectively. We considered three types of different natural DOM samples: the deep North Pacific Ocean, the oligotrophic surface of the North Pacific Ocean and porewater from the sulfidic tidal flats of the Wadden Sea. Bulk porewater and its individual polarity fractions presented the highest antioxidant activity and phenolic content. DOM from the water column samples had lower antioxidant activity and phenolic content than porewater, but exceeded what it is commonly found in macroalgae, microalgae, fruits and vegetables with cosmeceutical purposes. Our values were similar to published values for terrestrial DOM. The variations in bioactivity were dependent on polarity and molecular composition. The high resolution and high mass accuracy used to determine the molecular composition of marine DOM and the chemometric and multistatistical analyses employed have allowed to distinguish molecular categories that are related to the bioactive potential. As a future perspective, we performed cytotoxicity tests with human cells and propose marine DOM as a natural ingredient for the development of cosmeceutical products