Cloning of the gene KlABF2 in expression plasmids for "Saccharomyces cerevisiae”

Traballo fin de mestrado (UDC.CIE). Bioloxía molecular, celular e xenética. Curso 2013/201

Comerciants, pirates i conqueridors. Els víkings a l'Anglaterra del segle IX

Treballs Finals de Grau en Història, Facultat de Geografia i Història, Universitat de Barcelona, Curs: 2013-2014, Tutor: Daniel Piñol AlabartAquest treball tracta d’explicar el per què de les expansions víkingues i de l'arribada i assentament d'aquests a Anglaterra. Gràcies als descobriments de l'arqueologia dels últims 20 anys noves llums han aparegut al voltant de la magnitud de la migració escandinava a les illes, així com de la importància que van tenir en aquesta les dones, fins ara menyspreades. Per il·lustrar l'assentament víking he mostrat el cas de la ciutat de York, seu d'una important comunitat víkinga i amb prou restes materials per evidenciar l'impacte nòrdic a sòl anglès i la revifada de la vida del món urbà, després de dècades de decadència. He posat l'èmfasi en el paper de la dona, doncs és el camp que ha patit més canvis i controvèrsies, i en l'aplicació dels nous avenços científics, que han aportat una munió de dades que ha permès replantejar algunes idees i hipotetitzar-ne de noves. En referència a això i a la convivència entre víkings i anglosaxons, he parlat de dues foses comunes descobertes el 2008 i 2009, amb les restes de quasi un centenar d'individus que han estat identificats com a escandinaus, però entorn els quals s'ha obert un gran debat sobre si eren pirates, mercenaris o grangers, el que es consideraria un genocidi

Effects of climate on wood formation of Quercus robur L. and Quercus pyrenaica Willd. along a mediterraneity gradient in Galicia: an Integrated analysis using phenology, anatomy, and dendroecology

This Thesis is focused on the study of seasonal dynamics of wood formation and non-structural carbohydrates, as well as on the climatic information contained in tree-ring series, in two ring-porous species with contrasting ecological strategies (Q. robur L. and Q. pyrenaica Willd.) along a water-availability gradient in Galicia. This integrative approach allows us to yield a detailed portrayal of the processes controlling wood formation, and their consequences on tree hydraulic functionality and the carbon balance. This research is particularly concerned about the environmental control of growth, whereby the obtained information is used to ascertain the responses of deciduous oaks to the ongoing environmental change

A biometric and ecologic comparison between Artemia from Mexico and Chile

BACKGROUND: A preliminary biometric and ecologic database for the brine shrimp Artemia from Mexico and Chile is presented. The area abounds in small and seasonal ponds and large inland lakes, the latter mainly located in Mexico, although relatively large and isolated lakes are found in complex hydrological settings in pre-high plateau areas of Chile. This paper summarizes research efforts aimed at the localization, characterization, and evaluation of the aquaculture potential of Artemia populations in Mexico and Chile, which exhibit great habitat diversity (ponds, salterns, coastal lagoons, sea arms, coastal and inland lakes), contrasting weather conditions and different levels of isolation and human intervention. RESULTS: This study covered locations between 29° north latitude (Baja California, Mexico) to 50° south latitude (Puerto Natales, Chile). Biological characteristics considered are species name, reproductive mode, cyst diameter, chorion thickness, and nauplius length, whereas ecological data include pond size, pH, salinity, temperature, and water ionic composition. Artemia franciscana is the only species found in Mexico, it exists together with A. persimilis in Chile, though separated geographically. Ecological differences in habitat exist between both regions but also within countries, a pattern particularly clear with regard to water composition. Surprisingly, a Mexican (Cuatro Ciénegas, A. franciscana) and a Chilean location (Torres del Paine, A. persimilis) share habitat characteristics, at least for the period when data were collected. The discriminat analysis for cyst diameter and nauplius length shows that Artemia from only one location match in cyst diameter with those from San Francisco Bay (SFB) (Point Lobos), and one (Marquez) is far apart from SFB and all the others. The Chilean locations (Pampilla, Cejar, Cahuil, Llamara, Yape) share cyst diameter, but tend to differ from SFB. The remaining Mexican locations (Juchitan, Ohuira, Yavaros) are well separated from all the others. With regard to nauplii length, populations tend to distribute in a relatively random manner, being Marquez the location differing the most in cyst diameter from SFB. CONCLUSION: This database will contribute to the knowledge of radiation centers and serves as a baseline for further biogeographic studies, population characterization, management, and monitoring of Artemia biodiversity. Likewise, the impact of colonization and translocations for aquaculture purposes can be better assessed with a baseline for reference. Mexico and Chile exemplify the need to increase and further integrate regional information to tackle fundamental problems underlying practical utilization of Artemia

Applying A Methodology For Educating Students With Special Needs: A Case Study

The introduction of innovative educational technologies opens up new ways of interacting with students. We propose to exploit this potential to help in the education of children with special needs. We analyze the state of the art of tools supporting the teaching process, focusing on the omissions of existing research. We propose a new framework to help throughout the whole teaching process and describe its application to Proyecto Aprender (Learn Project), an educational resource targeting children with learning difficulties. Finally, we outline some conclusions and current/future research lines

The Endoplasmic Reticulum Glucosyltransferase Recognizes Nearly Native Glycoprotein Folding Intermediates

The UDP-Glc:glycoprotein glucosyltransferase (GT), a key player in the endoplasmic reticulum (ER) quality control of glycoprotein folding, only glucosylates glycoproteins displaying non-native conformations. To determine whether GT recognizes folding intermediates or irreparably misfolded species with nearly native structures, we generated and tested as GT substrates neoglycoprotein fragments derived from chymotrypsin inhibitor 2 (GCI2) bearing from 53 to 64 (full-length) amino acids. Fragment conformations mimicked the last stage-folding structures adopted by a glycoprotein entering the ER lumen. GT catalytic efficiency (V(max)/K(m)) remained constant from GCI2-(1-53) to GCI2-(1-58) and then steadily declined to reach a minimal value with GCI2-(1-64). The same parameter showed a direct hyperbolic relationship with solvent accessibility of the single Trp residue but only in fragments exposing hydrophobic amino acid patches. Mutations introduced (GCI2-(1-63)V63S and GCI2-(1-64)V63S) produced slight structural destabilizations but increased GT catalytic efficiency. This parameter presented an inverse exponential relationship with the free energy of unfolding of canonical and mutant fragments. Moreover, the catalytic efficiency showed a linear relationship with the fraction of unfolded species in water. It was concluded that the GT-derived quality control may be operative with nearly native conformers and that no alternative ER-retaining mechanisms are required when glycoproteins approach their proper folding.Fil: Caramelo, Julio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Castro, Olga Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: de Prat Gay, Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Parodi, Armando José A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentin

Optimización paramétrica y topológica de engranajes

En este trabajo se busca optimizar la relación peso-esfuerzo, es decir disminuir el peso manteniendo o aumentando en el mejor de los casos el esfuerzo que puedan soportar los engranajes rectos y helicoidales. Todo esto con la ayuda de la mecánica computacional y la simulación, con estas herramientas computacionales se obtienen resultados altamente fiables, sin la necesidad de fabricar o realizar ensayos mecánicos sobre las piezas, que no solo llevarían mucho tiempo, sino que también elevarían en gran medida los costos. la contribución de este estudio radica en la consecución de ahorro, de material sin repercutir negativamente en las propiedades mecánicas de la pieza, aumentando la eficacia de las maquinas en las que se utilizan.This paper seeks to optimize the weight-effort ratio, ie reduce weight while maintaining or increasing in the best effort that can withstand spur and helical gears. All this with the help of computational mechanics and simulation, computational tools with these highly reliable results without the need to manufacture or perform mechanical tests on the parts that not only would take a long time, but also greatly elevate the costs. the contribution of this study lies in achieving savings of material without adversely affect the mechanical properties of the workpiece, increasing the efficiency of the machines in which they are used

Sentido y otro (autrui) en Deleuze

La cuestión del otro (autrui) en Deleuze se confunde con la doble tarea de articular una nueva perspectiva sobre el sentido al mismo tiempo que cuestionar la imagen tradicional del pensar. Ello abre tempranamente un territorio para pensar dicha cuestión en su obra. Así, si bien el asunto es tratado sistemáticamente sólo a partir de Différence et répétition (1968) y Logique du sens (1969), ya en Proust et les signes (1964) se insinúan algunos de sus elementos fundamentales. En estas tres obras se juega, en efecto, la idea que nos interesa abordar en este estudio: la de que el territorio del otro es el sentido. Ello no evita introducir también algunas precisiones relativas al rostro y el modo como ello obliga a reformular los vínculos con el sentido. Lo sugerente del francés es que establece un tipo de pensamiento que se instala en la apertura al otro, pero sin que ello implique necesariamente entrar en el registro de la moral ni del conocimiento