The relationship between perception of child-rearing and self-esteem and its effect on perceived and actual scholastic competence in children

Elementary-school children (4th-6th grades)--Perceived parenting methods and scholastic achievement--Harter\u27s Self-Perception Profile--Parent-Child Relationship Questionnaire--Gender and ethnicity factors

STUDENT PERCEPTION ON FLEXIBLE SEATING AND THEIR ENGAGEMENT: A DESCRIPTIVE RESEARCH STUDY

This descriptive study examines students’ perspectives of their engagement when seated in flexible seating with choice. The researcher analyzed interview and survey data to answer the following research questions: 1.) What are students’ perceptions of flexible seating with choice? 2.) What are students’ perceptions on their level of engagement with flexible seating with choice? The purpose of this study was achieved by interviewing and surveying first grade students who have experienced flexible seating in their Title 1 school within Southern California. Using the data from the interviews, this study identified common themes around the perception of flexible seating among randomly selected students interviewed. Under the themes of comfort, freedom of choice, focus, movement, and feelings towards flexible seating were discussed. Using the data from the surveys, this study found that 75% of student participants perceived their level of engagement to be considered true engagement or full engagement according to Schlechty’s (2011) levels of engagement, while 18.8% perceived their level to be considered strategic compliance and 6.3% perceived theirs to be retreatism. This study provides insight into how students perceive their engagement with flexible seating. The results of this study, display that flexible seating has a positive relation to engagement and an important factor of this is student choice

Adaptation and Feasibility Study of a Digital Health Program to Prevent Diabetes among Low-Income Patients: Results from a Partnership between a Digital Health Company and an Academic Research Team.

Background. The feasibility of digital health programs to prevent and manage diabetes in low-income patients has not been adequately explored. Methods. Researchers collaborated with a digital health company to adapt a diabetes prevention program for low-income prediabetes patients at a large safety net clinic. We conducted focus groups to assess patient perspectives, revised lessons for improved readability and cultural relevance to low-income and Hispanic patients, conducted a feasibility study of the adapted program in English and Spanish speaking cohorts, and implemented real-time adaptations to the program for commercial use and for a larger trial of in multiple safety net clinics. Results. The majority of focus group participants were receptive to the program. We modified the curriculum to a 5th-grade reading level and adapted content based on patient feedback. In the feasibility study, 54% of eligible contacted patients expressed interest in enrolling (n = 23). Although some participants' computer access and literacy made registration challenging, they were highly satisfied and engaged (80% logged in at least once/week). Conclusions. Underserved prediabetic patients displayed high engagement and satisfaction with a digital diabetes prevention program despite lower digital literacy skills. The collaboration between researchers and a digital health company enabled iterative improvements in technology implementation to address challenges in low-income populations

Atopobium vaginae and Prevotella bivia are able to incorporate and influence gene expression in a pre-formed Gardnerella vaginalis biofilm

Bacterial vaginosis (BV) is associated with a highly structured polymicrobial biofilm on the vaginal epithelium where Gardnerella species presumably play a pivotal role. Gardnerella vaginalis, Atopobium vaginae, and Prevotella bivia are vaginal pathogens detected during the early stages of incident BV. Herein, we aimed to analyze the impact of A. vaginae and P. bivia on a pre-established G. vaginalis biofilm using a novel in vitro triple-species biofilm model. Total biofilm biomass was determined by the crystal violet method. We also discriminated the bacterial populations in the biofilm and in its planktonic fraction by using PNA FISH. We further analyzed the influence of A. vaginae and P. bivia on the expression of key virulence genes of G. vaginalis by quantitative PCR. In our tested conditions, A. vaginae and P. bivia were able to incorporate into pre-established G. vaginalis biofilms but did not induce an increase in total biofilm biomass, when compared with 48-h G. vaginalis biofilms. However, they were able to significantly influence the expression of HMPREF0424_0821, a gene suggested to be associated with biofilm maintenance in G. vaginalis. This study suggests that microbial relationships between co-infecting bacteria can deeply affect the G. vaginalis biofilm, a crucial marker of BV.This research was partially funded by the National Institute of Allergy and Infectious Diseases (R01AI146065-01A1). It was also partially funded by the Portuguese Foundation for Science and Technology (FCT), by the research project (PTDC/BIA-MIC/28271/2017), under the scope of COMPETE 2020 (POCI-01-0145-FEDER-028271), and by the strategic funding of unit (UIDB/04469/2020).info:eu-repo/semantics/publishedVersio

Exploring mathematical knowledge for teaching teachers: Supporting prospective elementary teachers’ relearning of mathematics

The growing number of studies on mathematics teacher educator knowledge have consistently argued that mathematics teacher educators require specialized knowledge in their work with prospective teachers (beyond the knowledge needed for teaching students), what researchers refer to as mathematical knowledge for teaching teachers. Drawing from existing research and aspects of our own work as mathematics teacher educators, we offer our own conceptualization of mathematical knowledge for teaching teachers and illustrate ways in which we as mathematics teacher educators use our own knowledge in teaching mathematics content to prospective teachers. We are particularly concerned with the knowledge mathematics teacher educators use to support prospective teachers’ relearning of mathematics, which involves prospective teachers ultimately reconstructing their previously developed knowledge of mathematics. We will illustrate ways in which we use various aspects of mathematical knowledge for teaching teachers to support prospective teachers’ relearning of mathematics through the lens of three different tasks of teaching. We conclude with a discussion of the implications of our analysis for informing the growing knowledge base for mathematics teacher educators

Otological complaints and concerns and communication difficulties of aged individuals

PURPOSE: To investigate the otological complaints and concerns of aged individuals, as well as the communication difficulties they face. METHODS: Fifty elderly subjects (eight men and 42 women) with ages ranging from 61 to 90 years were evaluated. All of them carried out an anamnesis and hearing tests, and presented symmetrical descending audiometric configuration. In this study, anamnesis data and the correlations between otological complaints and the degrees of hearing loss were presented and discussed. RESULTS: Hearing loss complaint was present in 70% of the sample and was associated to the concern about one's own hearing loss, with complaints of communication difficulties and also with the number situations in which the difficulty is observed (presence of background noise was the most cited aspect). Tinnitus complaint (52%) presented an association with concern involving this same aspect, whereas dizziness (38%), besides similar correlation, revealed an association with communication difficulty. There was a significant statistical association among degree of hearing loss in both low and middle frequencies and complaints of hearing loss and dizziness. In high frequencies, these correlations also occurred, along with a significant statistical association between degree of the hearing loss and complaint of communication difficulty. CONCLUSIONS: Of the otological complaints, hearing loss complaint was predominant among the elderly subjects of this study. Age and gender had no influence on the otological complaints and concerns. Degree of hearing loss influenced otological complaints of hearing loss, dizziness and communication difficulty.OBJETIVO: Investigar as queixas e as preocupações otológicas de indivíduos idosos, bem como as dificuldades de comunicação enfrentadas por esta população. MÉTODOS: Foram avaliados 50 idosos (61 a 90 anos de idade), sendo 8 homens e 42 mulheres, que apresentavam configuração audiométrica descendente simétrica. Os pacientes foram submetidos a uma entrevista e testes auditivos. Neste artigo, foram apresentados e discutidos os dados da entrevista e a correlação das queixas otológicas com os graus de perda auditiva. RESULTADOS: A queixa de perda auditiva esteve presente em 70% da amostra e revelou associação com a preocupação quanto à própria perda, com o queixa de dificuldade de comunicação e com a quantidade de situações nas quais a dificuldade é percebida, sendo a presença do ruído de fundo o aspecto mais citado. A queixa de zumbido (52%) apresentou associação com a preocupação envolvendo o próprio zumbido, enquanto que a tontura (38%), além de semelhante correlação, revelou associação com a dificuldade de comunicação. Ocorreu uma associação estatisticamente significante do grau da perda nas freqüências baixas e médias com a queixa de perda auditiva e de zumbido, enquanto que nas freqüências altas, além destas correlações, também ocorreu associação estatisticamente significante da perda auditiva com a queixa de dificuldade de comunicação. CONCLUSÕES: Dentre as queixas otológicas, a queixa de perda auditiva foi predominante entre os idosos. A idade e o gênero não influenciaram as queixas e as preocupações otológicas. O grau de perda auditiva influenciou as queixas otológicas de perda auditiva, de zumbido e a dificuldade de comunicação.Universidade Federal de São Paulo (UNIFESP)Universidade Federal de São Paulo (UNIFESP) Departamento de FonoaudiologiaHospital CEMAsLaboratório Delboni Auriemo Medicina DiagnósticaUNIFESP, Depto. de FonoaudiologiaSciEL

Bacterial vaginosis multi-species biofilms: can standard quantification methods accurately quantify in vitro biofilms?

Background. While it is well established that Bacterial Vaginosis (BV), the most common cause of vaginal discharge, involves the presence of a multi-species biofilm adhered to vaginal epithelial cells, in-depth study has been limited due to the complexity of the bacterial community comprising the biofilm. Assessing bacterial interactions between bacterial species that inhabit the BV biofilm can provide key information regarding synergism or antagonism between these species and provide insights into the pathogenesis of BV. Thus, proper biofilm quantification approaches are essential to further this body of research. Objectives. To evaluate BV biofilm formation by several key individual BV-associated bacteria (Gardnerella vaginalis, Fannyhessea vaginae, and Prevotella bivia) and compare with a multispecies biofilm formed simultaneously by all three bacterial species. Methods. Single- or multi-species biofilms were quantified by the crystal violet (CV) staining method, total cell counts by epifluorescence microscopy, and the plate counting technique (CFU); individual traits were assessed by bacterial species. Results. Each individual species had a unique signature assessed by the distinct relationship between the total number of cells, CFUs, and total biofilm biomass. Conclusions & Significance: The assessment of multi-species BV biofilm quantification results in significant bias, mainly since individual species quantification signatures cant be related to the multi-species consortia. To minimize this bias, a multiple-technical approach should be considered when quantifying multi-species BV biofilms, to circumvent the caveats of individual techniques alone, tailoring a more complete picture of the biofilm-forming capacity of key bacterial species and furthering the field of BV pathogenesis research.This work was partially supported by the Portuguese Foundation for Science and Technology (FCT) by the research project [PTDC/BIA-MIC/28271/2017] under the scope of COMPETE 2020 [POCI-01-0145-FEDER 028271] and by the strategic funding of unit [UID/BIO/04469/2020]. It was also partially funded by the National Institute of Allergy and Infectious Diseases (R01AI146065-01A1)info:eu-repo/semantics/publishedVersio

Effect of pH and volume in the hatching of resistance cysts of the species Dendrocephalus brasiliensis Pesta, 1921 (Crustacea, Anostraca)

Branchoneta is an option as live food for aquaculture and it is necessary to develop more studies to get information that makes possible its culture on a large scale. Our aim was to establish a method that results in a higher percentage hatching of D. brasiliensis. We analyzed if the cyst density could cause any significant difference in hatching; for that test we use 2 different conditions: (I) 15 ml glass tube (T1, T2, and T3) with 25 (twenty-five) cysts/repetition; and (II)  Erlenmeyer of 150 ml (T4, T5, and T6) with 25 cysts/repetition totaling 75 cysts/treatment, with triplicates to all treatments. We also tested 3 different pH conditions: acid (pH 3), neutral (pH~7/distilled water) and alkaline (pH 8), all in natural light and temperature. We conclude that there is no difference between the treatments, for none of the conditions tested. But other results have to be considered as Hatching Speed index and the Average Hatching Time with best results for the treatments T3, T1, and T2, respectively. The density of 0.6 ml/cyst (glass tube) resulted in faster hatching, which shows the necessity of further studies to analyze the speed of hatching under different conditions of density. The relative frequency showed that the hatchings peak occurs in the second day. We conclude that pH and density, in this study, not influenced the beginning of the hatching process

Microbial Monitoring from the Frontlines to Space: Department of Defense Small Business Innovation Research Technology Aboard the International Space Station

The RAZOR (trademark) EX, a quantitative Polymerase Chain Reaction (qPCR) instrument, is a portable, ruggedized unit that was designed for the Department of Defense (DoD) with its reagent chemistries traceable to a Small Business Innovation Research (SBIR) contract beginning in 2002. The PCR instrument's primary function post 9/11 was to enable frontline soldiers and first responders to detect biological threat agents and bioterrorism activities in remote locations to include field environments. With its success for DoD, the instrument has also been employed by other governmental agencies including Department of Homeland Security (DHS). The RAZOR (Trademark) EX underwent stringent testing by the vendor, as well as through the DoD, and was certified in 2005. In addition, the RAZOR (trademark) EX passed DHS security sponsored Stakeholder Panel on Agent Detection Assays (SPADA) rigorous evaluation in 2011. The identification and quantitation of microbial pathogens is necessary both on the ground as well as during spaceflight to maintain the health of astronauts and to prevent biofouling of equipment. Currently, culture-based monitoring technology has been adequate for short-term spaceflight missions but may not be robust enough to meet the requirements for long-duration missions. During a NASA-sponsored workshop in 2011, it was determined that the more traditional culture-based method should be replaced or supplemented with more robust technologies. NASA scientists began investigating innovative molecular technologies for future space exploration and as a result, PCR was recommended. Shortly after, NASA sponsored market research in 2012 to identify and review current, commercial, cutting edge PCR technologies for potential applicability to spaceflight operations. Scientists identified and extensively evaluated three candidate technologies with the potential to function in microgravity. After a thorough voice-of-the-customer trade study and extensive functional and safety evaluations, the RAZOR (trademark) EX PCR instrument(Bio-Fire Defense, Salt Lake City, UT) was selected as the most promising current technology for spaceflight monitoring applications