Polarimetry of Li-rich giants

Protoplanetary nebulae typically present non-spherical envelopes. The origin of such geometry is still controversial. There are indications that it may be carried over from an earlier phase of stellar evolution, such as the AGB phase. But how early in the star's evolution does the non-spherical envelope appear? Li-rich giants show dusty circumstellar envelopes that can help answer that question. We study a sample of fourteen Li-rich giants using optical polarimetry in order to detect non-spherical envelopes around them. We used the IAGPOL imaging polarimeter to obtain optical linear polarization measurements in V band. Foreground polarization was estimated using the field stars in each CCD frame. After foreground polarization was removed, seven objects presented low intrinsic polarization (0.19 - 0.34)% and two (V859 Aql and GCSS 557) showed high intrinsic polarization values (0.87 - 1.16)%. This intrinsic polarization suggests that Li-rich giants present a non-spherical distribution of circumstellar dust. The intrinsic polarization level is probably related to the viewing angle of the envelope, with higher levels indicating objects viewed closer to edge-on. The correlation of the observed polarization with optical color excess gives additional support to the circumstellar origin of the intrinsic polarization in Li-rich giants. The intrinsic polarization correlates even better with the IRAS 25 microns far infrared emission. Analysis of spectral energy distributions for the sample show dust temperatures for the envelopes tend to be between 190 and 260 K. We suggest that dust scattering is indeed responsible for the optical intrinsic polarization in Li-rich giants. Our findings indicate that non-spherical envelopes may appear as early as the red giant phase of stellar evolution.Comment: to be published in A&A, 15 pages, 10 figures. Fig. 3 is available in ftp://astroweb.iag.usp.br/pub/antonio/4270/4270.fig3.pd

Three Li-rich K giants: IRAS 12327-6523, IRAS 13539-4153, and IRAS 17596-3952

We report on spectroscopic analyses of three K giants previously suggested to be Li-rich: IRAS 12327-6523, IRAS 13539-4153, and IRAS 17596-3952. High-resolution optical spectra and the LTE model atmospheres are used to derive the stellar parameters: ($T_{\rm eff}$, log $g$, [Fe/H]), elemental abundances, and the isotopic ratio $^{12}$C/$^{13}$C. IRAS 13539-4153 shows an extremely high Li abundance of $\log\epsilon$(Li) $\approx$ 4.2, a value ten times more than the present Li abundance in the local interstellar medium. This is the third highest Li abundance yet reported for a K giant. IRAS 12327-6523 shows a Li abundances of $\log\epsilon$(Li)$\approx$ 1.4. IRAS 17596-3952 is a rapidly rotating ($V{\sin i}$ $\approx$ 35 km s$^{-1}$) K giant with $\log\epsilon$(Li) $\approx$ 2.2. Infrared photometry which shows the presence of an IR excess suggesting mass-loss. A comparison is made between these three stars and previously recognized Li-rich giants.Comment: 17 pages, 6 figures, accepted for A

Restoring observed classical behavior of the carbon nanotube field emission enhancement factor from the electronic structure

Experimental Fowler-Nordheim plots taken from orthodoxly behaving carbon nanotube (CNT) field electron emitters are known to be linear. This shows that, for such emitters, there exists a characteristic field enhancement factor (FEF) that is constant for a range of applied voltages and applied macroscopic fields $F_\text{M}$. A constant FEF of this kind can be evaluated for classical CNT emitter models by finite-element and other methods, but (apparently contrary to experiment) several past quantum-mechanical (QM) CNT calculations find FEF-values that vary with $F_\text{M}$. A common feature of most such calculations is that they focus only on deriving the CNT real-charge distributions. Here we report on calculations that use density functional theory (DFT) to derive real-charge distributions, and then use these to generate the related induced-charge distributions and related fields and FEFs. We have analysed three carbon nanostructures involving CNT-like nanoprotrusions of various lengths, and have also simulated geometrically equivalent classical emitter models, using finite-element methods. We find that when the DFT-generated local induced FEFs (LIFEFs) are used, the resulting values are effectively independent of macroscopic field, and behave in the same qualitative manner as the classical FEF-values. Further, there is fair to good quantitative agreement between a characteristic FEF determined classically and the equivalent characteristic LIFEF generated via DFT approaches. Although many issues of detail remain to be explored, this appears to be a significant step forwards in linking classical and QM theories of CNT electrostatics. It also shows clearly that, for ideal CNTs, the known experimental constancy of the FEF value for a range of macroscopic fields can also be found in appropriately developed QM theory.Comment: A slightly revised version has been published - citation below - under a title different from that originally used. The new title is: "Restoring observed classical behavior of the carbon nanotube field emission enhancement factor from the electronic structure

Evaluation of IRES-mediated expression and different signal peptides for the development of CHO clones producing an anti-PCSK9 monoclonal antibody

High levels of low-density lipoprotein (LDL) in the blood are associated to an increased risk of cardiovascular diseases, which are a leading cause of death worldwide. Despite this scenario, only minor progress was made since the introduction of statins as lipid-lowering synthetic drugs in the 1980s. Among patients suffering from high cholesterol levels, about 20% present strong adverse effects to statins or don’t manage to decrease their LDL levels to normality. Since 2015, a new class of monoclonal antibodies (mAbs) was approved by FDA and EMA. These mAbs target the enzyme PCSK9 that is involved in the regulation of LDL cholesterol homeostasis, allow a higher amount of LDL receptors to be available on the surface of hepatic cells and thus enable a significant reduction of the circulating cholesterol levels. In this study, we selected signal peptides and IRES elements from the literature to use for the construction of different tricistronic expression vectors. For the light chain, two different signal peptides were evaluated (SP1 and SP2), whereas for the heavy chain just one signal peptide was used (SP3). An EMCV IRES element was placed between the light chain (LC) gene and SP3. Downstream of the heavy chain (HC) gene, an attenuated IRES (att-IRES) element was inserted, followed by the antibiotics resistance gene for selection. In order to enable the evaluation of a stringent double selection (two different antibiotics) upon co-transfection with two plasmids, two sets of vectors were constructed, having either geneticin (neo) or hygromycin B as selection marker. Thus, a total of 4 vectors were constructed, having the following tricistronic cassette structure: CMV-IE promoter, SP1 or SP2, LC gene, EMCV IRES, SP3, HC gene, att-IRES, neo or hygro. CHO.K1 (ATCC, USA) previously adapted to suspension culture in TC-LECC medium (Xell AG, Germany) were transfected (or co-transfected) using Lipofectamine 3000 (Gibco, USA), using either circular or linearized plasmids. Cell culture supernatants harvested 48h post transfection showed a similar expression level for all constructs. After approximately 2 months under selection pressure with the respective antibiotics, the stable cell pool that had been transfected with circular SP1-LC-SP3-HC-NEO was chosen for further studies. Cells were cultivated in shake flasks, attaining 13.9E6 cells/mL on day 7 and showing an specific growth rate of 1.2 d-1 during the exponential growth phase. The mAb was purified from cell culture supernatant using protein A affinity chromatography, showing high purity and homogeneity. Affinity of the purified mAb to PCSK9 was tested, confirming the success of the approach adopted in this work

Models of Metal Poor Stars with Gravitational Settling and Radiative Accelerations: I. Evolution and Abundance Anomalies

Evolutionary models have been calculated for Pop II stars of 0.5 to 1.0$M_\odot$ from the pre-main-sequence to the lower part of the giant branch. Rosseland opacities and radiative accelerations were calculated taking into account the concentration variations of 28 chemical species, including all species contributing to Rosseland opacities in the OPAL tables. The effects of radiative accelerations, thermal diffusion and gravitational settling are included. While models were calculated both for Z=0.00017 and 0.0017, we concentrate on models with Z=0.00017 in this paper. These are the first Pop II models calculated taking radiative acceleration into account. It is shown that, at least in a 0.8$M_\odot$ star, it is a better approximation not to let Fe diffuse than to calculate its gravitational settling without including the effects of $g_{rad}(Fe)$. In the absence of any turbulence outside of convection zones, the effects of atomic diffusion are large mainly for stars more massive than 0.7$M_\odot$. Overabundances are expected in some stars with \teff \ge 6000K. Most chemical species heavier than CNO are affected. At 12 Gyr, overabundance factors may reach 10 in some cases (e.g. for Al or Ni) while others are limited to 3 (e.g. for Fe). The calculated surface abundances are compared to recent observations of abundances in globular clusters as well as to observations of Li in halo stars. It is shown that, as in the case of Pop I stars, additional turbulence appears to be present.Comment: 40 pages, 17 color figures, to appear in The Astrophysical Journal, April 2002 (paper with original high resolution figures can be found at http://www.cerca.umontreal.ca/~richer/Fichiersps/popII_1.ps

Detailed Analysis of Nearby Bulgelike Dwarf Stars II. Lithium Abundances

Li abundances are derived for a sample of bulgelike stars with isochronal ages of 10-11 Gyr. These stars have orbits with pericentric distances, Rp, as small as 2-3 kpc and Zmax < 1 kpc. The sample comprises G and K dwarf stars in the metallicity range -0.80<[Fe/H]< +0.40. Few data of Li abundances in old turn-off stars (> 4.5 Gyr) within the present metallicity range are available. M67 (4.7 Gyr) and NGC 188 (6 Gyr) are the oldest studied metal-rich open clusters with late-type stars. Li abundances have also been studied for few samples of old metal-rich field stars. In the present work a high dispersion in Li abundances is found for bulgelike stars for all the metallicity range, comparable with values in M67. The role of metallicity and age on a Li depletion pattern is discussed. The possible connection between Li depletion and oxygen abundance due to atmospheric opacity effects is investigated.Comment: 9 pages, 7 figure

O ensino de Zoologia evolutiva na educação básica : uma experiência desenvolvida nos primeiros anos do ensino fundamental

A Zoologia ensinada desde as séries iniciais distancia-se das atuais propostas para um ensino evolutivo. As visões utilitaristas e antropocêntricas sobre os animais são prevalentes e dificultam a formação de conceitos e a aprendizagem sobre Evolução em séries escolares posteriores. Com basesnesses pressupostos foi desenvolvida uma pesquisa de intervenção com 16 alunos de 3º ano do Ensino Fundamental, objetivando-se identificar, descrever e analisar os aspectos potenciais e limitantes de um processo educativo de Zoologia Evolutiva nas séries iniciais da escola básica, utilizando-se fundamentos teórico-práticos de Ausubel e Vigotski. As conclusões dessa experiência orientam-se para uma real e consistente possibilidade de aprendizagem significativa de Zoologia Evolutiva nas séries iniciais

Inhibition Of Mitochondrial Permeability Transition By Low Ph Is Associated With Less Extensive Membrane Protein Thiol Oxidation.

Ca2+ and inorganic phosphate-induced mitochondrial swelling and membrane protein thiol oxidation, which are associated with mitochondrial permeability transition, are inhibited by progressively decreasing the incubation medium pH between 7.2 and 6.0. Nevertheless, the detection of mitochondrial H2O2 production under these conditions is increased. Permeability transition induced by phenylarsine oxide, which promotes membrane protein thiol cross-linkage in a process independent of Ca2+ or reactive oxygen species, is also strongly inhibited in acidic incubation media. In addition, we observed that the decreased protein thiol reactivity with phenylarsine oxide or phenylarsine oxide-induced swelling at pH 6.0 is reversed by diethyl pyrocarbonate, in a hydroxylamine-sensitive manner. These results provide evidence that the inhibition of mitrochondrial permeability transition observed at lower incubation medium pH is mediated by a decrease in membrane protein thiol reactivity, related to the protonation of protein histidyl residues.19525-3

The paradox of retained genetic diversity of Hippocampus guttulatus in the face of demographic decline

Genetic diversity is the raw foundation for evolutionary potential. When genetic diversity is significantly reduced, the risk of extinction is heightened considerably. The long-snouted seahorse (Hippocampus guttulatus) is one of two seahorse species occurring in the North-East Atlantic. The population living in the Ria Formosa (South Portugal) declined dramatically between 2001 and 2008, prompting fears of greatly reduced genetic diversity and reduced effective population size, hallmarks of a genetic bottleneck. This study tests these hypotheses using samples from eight microsatellite loci taken from 2001 and 2013, on either side of the 2008 decline. The data suggest that the population has not lost its genetic diversity, and a genetic bottleneck was not detectable. However, overall relatedness increased between 2001 to 2013, leading to questions of future inbreeding. The effective population size has seemingly increased close to the threshold necessary for the population to retain its evolutionary potential, but whether these results have been affected by sample size is not clear. Several explanations are discussed for these unexpected results, such as gene flow, local decline due to dispersal to other areas of the Ria Formosa, and the potential that the duration of the demographic decline too short to record changes in the genetic diversity. Given the results presented here and recent evidence of a second population decline, the precise estimation of both gene flow and effective population size via more extensive genetic screening will be critical to effective population management.16-02-01-FMP-54, MAR-01.04.02-FEAMP-0029, UID/Multi/04326/2020info:eu-repo/semantics/publishedVersio