Analysis of Student Perceptions of the Psychosocial Learning Environment in Online and Face-to-Face Career and Technical Education Courses

Career and technical education (CTE) courses offered online are becoming more common in secondary schools. Recognizing the adaptability of CTE courses and designing curricula to offer online learners the same experience as face-to-face learners is challenging for education professionals and requires analyses of both environments. A lack of empirical studies makes it important to conduct research on online learning environments from the perspectives of high school students. This study analyzes student perceptions of the psychosocial learning environment in online and face-to-face career and technical education courses. The research explores and compares how high school students perceive their learning environment and should help online course developers in the preparation of effective courses. This study used existing survey data from a school district in Washington State from the 2013/2014 school year. The instrument used in this study was the Distance Education Learning Environment Survey (DELES) which was modified and revalidated for use with high school students. Statistical analysis included an examination of the sum of the mean scores and standard deviations of the survey\u27s seven scale areas using face-to-face and online student data. A Mann-Whitney U test was used to measure variability and compare the sum of the mean scores of each of the scales between online and face-to-face environments to determine if differences exist. Analysis of the data from this study indicated that in the areas of active learning and autonomy, students perceived online education as offering more benefit than face-to-face education. In the areas of student interaction and collaboration and enjoyment, student perceptions favored the face-to-face environment

The Evolution of Host Specialization in the Vertebrate Gut Symbiont Lactobacillus reuteri

Recent research has provided mechanistic insight into the important contributions of the gut microbiota to vertebrate biology, but questions remain about the evolutionary processes that have shaped this symbiosis. In the present study, we showed in experiments with gnotobiotic mice that the evolution of Lactobacillus reuteri with rodents resulted in the emergence of host specialization. To identify genomic events marking adaptations to the murine host, we compared the genome of the rodent isolate L. reuteri 100-23 with that of the human isolate L. reuteri F275, and we identified hundreds of genes that were specific to each strain. In order to differentiate true host-specific genome content from strain-level differences, comparative genome hybridizations were performed to query 57 L. reuteri strains originating from six different vertebrate hosts in combination with genome sequence comparisons of nine strains encompassing five phylogenetic lineages of the species. This approach revealed that rodent strains, although showing a high degree of genomic plasticity, possessed a specific genome inventory that was rare or absent in strains from other vertebrate hosts. The distinct genome content of L. reuteri lineages reflected the niche characteristics in the gastrointestinal tracts of their respective hosts, and inactivation of seven out of eight representative rodent-specific genes in L. reuteri 100-23 resulted in impaired ecological performance in the gut of mice. The comparative genomic analyses suggested fundamentally different trends of genome evolution in rodent and human L. reuteri populations, with the former possessing a large and adaptable pan-genome while the latter being subjected to a process of reductive evolution. In conclusion, this study provided experimental evidence and a molecular basis for the evolution of host specificity in a vertebrate gut symbiont, and it identified genomic events that have shaped this process

Novel Role for p110β PI 3-Kinase in Male Fertility through Regulation of Androgen Receptor Activity in Sertoli Cells

We thank Anna-Lena Berg (AstraZeneca, Lund) and Cheryl Scudamore (MRC, Harwell, UK) for histological analysis, Julie Foster (Barts Cancer Institute, London) for CT scans, Johan Swinnen and Frank Claessens (Leuven University, Belgium) for discussion and AR-luciferase reporter plasmids, Florian Guillou (INRA, CNRS, Université de Tours, France) for the AMH-Cre mouse line and Laura Milne (MRC Centre for Reproductive Health, The University of Edinburgh) for technical support. We thank the members of the Cell Signalling group for critical input.International audienceThe organismal roles of the ubiquitously expressed class I PI3K isoform p110β remain largely unknown. Using a new kinase-dead knockin mouse model that mimics constitutive pharmacological inactivation of p110β, we document that full inactivation of p110β leads to embryonic lethality in a substantial fraction of mice. Interestingly, the homozygous p110β kinase-dead mice that survive into adulthood (maximum ~26% on a mixed genetic background) have no apparent phenotypes, other than subfertility in females and complete infertility in males. Systemic inhibition of p110β results in a highly specific blockade in the maturation of spermatogonia to spermatocytes. p110β was previously suggested to signal downstream of the c-kit tyrosine kinase receptor in germ cells to regulate their proliferation and survival. We now report that p110β also plays a germ cell-extrinsic role in the Sertoli cells (SCs) that support the developing sperm, with p110β inactivation dampening expression of the SC-specific Androgen Receptor (AR) target gene Rhox5, a homeobox gene critical for spermatogenesis. All extragonadal androgen-dependent functions remain unaffected by global p110β inactivation. In line with a crucial role for p110β in SCs, selective inactivation of p110β in these cells results in male infertility. Our study is the first documentation of the involvement of a signalling enzyme, PI3K, in the regulation of AR activity during spermatogenesis. This developmental pathway may become active in prostate cancer where p110β and AR have previously been reported to functionally interac

Factors Predicting Relationship Satisfaction, Investment, and Commitment When Women Report High Prevalence of Psychological Abuse

Relationship satisfaction, investment, and commitment in women experiencing extensive psychological abuse were examined to determine predictive factors. Participants were the top quartile of a national sample of women in conflictual relationships (N = 81) experiencing psychological maltreatment. Relationship satisfaction, investment, and commitment were each used as criterion variables with conceptually related factors as the predictors (e.g., reactions to the psychological abuse; mental health indicators; personality variables; perceived harm; problematic relationship schemas; response styles; demographics). These relationship markers were differentially predicted within this group of women. Three independent clusters of women, based on patterns of satisfaction, investment, and commitment scores, were compared regarding physical abuse, reactions to psychological abuse, ratings of perceived harm, endorsement of relationship schemas, psychological distress variables, and personality variables. The cluster of women reporting higher satisfaction, investment, and commitment in these seemingly aversive relationships frequently demonstrated differences from the other two clusters. Implications for clinical applications are discussed