Doping of Si nanoparticles: the effect of oxidation

The preferred location of boron and phosphorus in oxidized free-standing Si nanoparticles was investigated using a first-principles density functional approach. The calculated formation energies indicate that P should segregate to the silicon core, whereas B is equally stable in the Si and SiO_2 regions. Our models thus suggest that, in contrast with nanocrystals with H-terminated surfaces, the efficiency of phosphorus incorporation in oxidized Si nanoparticles can be improved by thermal annealing

Introduction of exogenous AMF species alters the biological diversity and functionality of AMF communities associated with cowpea

The salinity in arid and semi-arid areas of the world is rapidly expanding due to climate change and anthropogenic activities. The use of inoculants containing beneficial microbes (e.g. arbuscular mycorrhizal fungi (AMF) and rhizobia) is a promising alternative to improve plant production in these regions. Here, we investigated the effect of common agricultural practices such as the use of beneficial microbes as inoculum and crop rotation on cowpea growth and on its association with soil microbes under non- and salt-stressed conditions. Plant experiments were carried out using non-sterilized soil (supplemented or not with NaCl) under greenhouse conditions. Bradyrhizobium yuanmingense BR 3267 strain and a commercial mixture of AMF (Endoplant Riego) were used as inoculants. In parallel, we assessed cowpea growth following succession of buffelgrass (Cenchrus ciliaris) with or without prior soil disturbance. Plant and symbiotic parameters, nutrient content in leaves and AMF and root nodule communities through DNA metabarcoding were evaluated. Under non-stressed conditions, inoculation with AMF and/or BR 3267 strain led to significant increase of cowpea biomass production and higher N or P content in leaves. The imposed saline condition affected the cowpea growth although without significantly affecting the symbiotic parameters. Moreover, the increase of AMF propagules available in the soil at buffelgrass sowing through the inoculation of commercial AMF was a determining factor to mitigate the effects of soil tillage and salinity on cowpea growth. The bacterial communities in the root nodules were affected by AMF communities rather by rhizobia inoculation. Benefits of commercial AMF could be explained by changes in the biological and functionality of the AMF communities associated with cowpea. This study reveals that microbial inoculation and crop rotation are effective practices for improvement of cowpea growth and on mitigating the harmful effects of salt

Dupla inoculação (rizóbio e fungos micorrízicos arbusculares) mitigam efeitos nocivos da salinidade no crescimento do feijão-caupi

A salinidade em áreas áridas e semiáridas do mundo está a expandir rapidamente devido às alterações climáticas e atividades humanas. O uso de inóculos contendo microrganismos benéficos, como fungos micorrízicos arbusculares (FMA) e bactérias simbióticas fixadoras de azoto, aliado ao manejo adequado do solo e das culturas, é uma alternativa promissora para melhorar a produção vegetal nessas regiões. Aqui, investigámos o efeito de práticas agrícolas comuns, nomeadamente a utilização de microrganismos benéficos como inóculo e a perturbação do solo entre a rotação de culturas, no crescimento do feijão-caupi e na sua associação com micróbios do solo sob condições salinas e não salinas. A estirpe Bradyrhizobium yuanmingense BR 3267, e uma mistura comercial de FMA (Endoplant Riego) foram utilizados como inóculos. Paralelamente, avaliámos o crescimento do feijão-caupi, após sucessão ao capim-buffel com ou sem perturbação prévia do solo. Ensaios de plantas em solo não-esterilizado (suplementado ou não com NaCl) foram realizados em dois ciclos de 75 dias em estufa. Parâmetros vegetais e simbióticos e o teor de nutrientes nas folhas foram determinados, bem como a diversidade bacteriana e de FMA em raízes e nódulos de feijão-caupi por meio de sequenciamento DNA metabarcode. Os nossos dados revelaram que os parâmetros simbióticos (número de nódulos e/ou taxa de colonização) foram melhorados no feijão-caupi inoculado com Bradyrhizobium e/ou FMA comercial, o que consequentemente resultou em maior teor de N ou P nas folhas. A salinidade imposta afetou negativamente o crescimento do feijão, porém sem afetar significativamente os parâmetros simbióticos analisados. O aumento de propágulos de FMA disponíveis no solo através da inoculação de FMA comercial foi um fator determinante para mitigar os efeitos do manejo do solo no crescimento do feijão. A perturbação do solo mostrou impacto negativo nos parâmetros vegetais e simbióticos, exceto para o número de nódulos. Além disso, os efeitos positivos do uso de FMA comercial no crescimento do feijão-caupi, em condições controlo ou de salinidade podem ser explicados pelas mudanças na funcionalidade das comunidades de FMA associadas ao feijão-caupi. Por outro lado, as comunidades bacterianas em nódulos ou raízes do feijão não foram afetadas pela inoculação de FMA ou de rizóbios. Este estudo revela que a dupla inoculação (mistura de rizóbio e FMA) e a rotação de culturas, sem a perturbação do solo, são práticas eficazes para melhorar o crescimento do feijão-caupi e mitigar os efeitos nocivos do sal no seu crescimento. Mais, estes resultados também sugerem que os efeitos sinérgicos entre rizóbios e FMA dependem principalmente de um estabelecimento bem-sucedido da simbiose entre FMA e a planta hospedeira e não da comunidade de FMA em si

Profiling of lung microbiota discloses differences in adenocarcinoma and squamous cell carcinoma

The lung is a complex ecosystem of host cells and microbes often disrupted in pathological conditions. Although bacteria have been hypothesized as agents of carcinogenesis, little is known about microbiota profile of the most prevalent cancer subtypes: adenocarcinoma (ADC) and squamous cell carcinoma (SCC). To characterize lung cancer (LC) microbiota a first a screening was performed through a pooled sequencing approach of 16S ribosomal RNA gene (V3-V6) using a total of 103 bronchoalveaolar lavage fluid samples. Then, identified taxa were used to inspect 1009 cases from The Cancer Genome Atlas and to annotate tumor unmapped RNAseq reads. Microbial diversity was analyzed per cancer subtype, history of cigarette smoking and airflow obstruction, among other clinical data. We show that LC microbiota is enriched in Proteobacteria and more diverse in SCC than ADC, particularly in males and heavier smokers. High frequencies of Proteobacteria were found to discriminate a major cluster, further subdivided into well-defined communities’ associated with either ADC or SCC. Here, a SCC subcluster differing from other cases by a worse survival was correlated with several Enterobacteriaceae. Overall, this study provides first evidence for a correlation between lung microbiota and cancer subtype and for its influence on patient life expectancy.We would like to thank all patients for donating their samples and for collaborating in this study. IPATIMUP integrates the i3S Research Unit, which is partially supported by the Portuguese Foundation for Science and Technology (FCT). This work was supported by the Portuguese Foundation for Science and Technology (FCT), financed by the European Social Funds (COMPETE-FEDER) and National Funds through the FCT (projects PEstC/SAU/LA0003/2013 and POCI-01-0145-FEDER-007274, fellowships SFRH/BPD/77646/2011 and SFRH/BPD/120777/2016 to S.G. and P.I.M., respectively, grant PTDC/BEXGMG/0242/2012 to S.S. and by Programa Operacional Regional do Norte (ON.2 – O Novo Norte and Norte 2020), through FEDER funds under the Quadro de Referência Estratégico Nacional (QREN; projects NORTE-07-0162-FEDER-00018 and NORTE-070162-FEDER-000067, and NORTE-01-0145-FEDER-000029)

Evidence That Lipopolisaccharide May Contribute to the Cytokine Storm and Cellular Activation in Patients with Visceral Leishmaniasis

Visceral leishmaniasis (VL) affects organs rich in lymphocytes, being characterized by intense Leishmania-induced T-cell depletion and reduction in other hematopoietic cells. In other infectious and non-infectious diseases in which the immune system is affected, such as HIV-AIDS and inflammatory bowel disease, damage to gut-associated lymphocyte tissues occurs, enabling luminal bacteria to enter into the circulation. Lipopolisaccharide (LPS) is a bacterial product that stimulates macrophages, leading to the production of pro-inflammatory cytokines and other soluble factors such as MIF, which in turn activate lymphocytes. Continuous and exaggerated stimulation causes exhaustion of the T-cell compartment, contributing to immunosuppression

Horava-Lifshitz Holography

We derive the detailed balance condition as a solution to the Hamilton-Jacobi equation in the Horava-Lifshitz gravity. This result leads us to propose the existence of the d-dimensional quantum field theory on the future boundary of the (d+1)-dimensional Horava-Lifshitz gravity from the viewpoint of the holographic renormalization group. We also obtain a Ricci flow equation of the boundary theory as the holographic RG flow, which is the Hamilton equation in the bulk gravity, by tuning parameters in the theory.Comment: 7 page

Measurement of the J/Psi Production Cross Section in 920 GeV/c Fixed-Target Proton-Nucleus Interactions

The mid-rapidity (dsigma_(pN)/dy at y=0) and total sigma_(pN) production cross sections of J/Psi mesons are measured in proton-nucleus interactions. Data collected by the HERA-B experiment in interactions of 920 GeV/c protons with carbon, titanium and tungsten targets are used for this analysis. The J/Psi mesons are reconstructed by their decay into lepton pairs. The total production cross section obtained is sigma_(pN)(J/Psi) = 663 +- 74 +- 46 nb/nucleon. In addition, our result is compared with previous measurements

Effect of energy restriction and physical exercise intervention on phenotypic flexibility as examined by transcriptomics analyses of mRNA from adipose tissue and whole body magnetic resonance imaging

Overweight and obesity lead to changes in adipose tissue such as inflammation and reduced insulin sensitivity. The aim of this study was to assess how altered energy balance by reduced food intake or enhanced physical activity affect these processes. We studied sedentary subjects with overweight/obesity in two intervention studies, each lasting 12 weeks affecting energy balance either by energy restriction (~20% reduced intake of energy from food) in one group, or by enhanced energy expenditure due to physical exercise (combined endurance- and strength-training) in the other group. We monitored mRNA expression by microarray and mRNA sequencing from adipose tissue biopsies. We also measured several plasma parameters as well as fat distribution with magnetic resonance imaging and spectroscopy. Comparison of microarray and mRNA sequencing showed strong correlations, which were also confirmed using RT-PCR. In the energy restricted subjects (body weight reduced by 5% during a 12 weeks intervention), there were clear signs of enhanced lipolysis as monitored by mRNA in adipose tissue as well as plasma concentration of free-fatty acids. This increase was strongly related to increased expression of markers for M1-like macrophages in adipose tissue. In the exercising subjects (glucose infusion rate increased by 29% during a 12-week intervention), there was a marked reduction in the expression of markers of M2-like macrophages and T cells, suggesting that physical exercise was especially important for reducing inflammation in adipose tissue with insignificant reduction in total body weight. Our data indicate that energy restriction and physical exercise affect energy-related pathways as well as inflammatory processes in different ways, probably related to macrophages in adipose tissue.</p

Search for the Flavor-Changing Neutral Current Decay D0→μ+μ−D^0 \to \mu^+\mu^- with the HERA-B Detector

We report on a search for the flavor-changing neutral current decay $D^0 \to \mu^+\mu^-$ using $50 \times 10^6$ events recorded with a dimuon trigger in interactions of 920 GeV protons with nuclei by the HERA-B experiment. We find no evidence for such decays and set a 90% confidence level upper limit on the branching fraction $Br(D^0 \to \mu^+\mu^-) <2.0 \times 10^{-6}$.Comment: 17 pages, 4 figures (of which 1 double), paper to be submitted to Physics Letters