Development of Healthy Protein-Rich Crackers Using Tenebrio molitor Flour

Entomophagy is still a widespread practice in Africa and Asia, although it is declining due to the westernization of diets. Today, the issue of its rehabilitation is underway; indeed, the nutritional economic and ecological stakes of this consumption are strategic. It can be considered an important way to face the scarcity of natural resources, environmental pressures due to the increasing world population, and demand for protein. Tenebrio molitor larvae flour was recently approved by the European Food Safety Authority (EFSA) as a novel food. The aim of the present work was to create protein-rich healthy cracker from insect flour, achieving the claim “source of protein” with a target market focused on the healthy products for consumption on the go. Contents of T. molitor flour from 2 to 20% (%w/w) were tested, using a previously optimized formulation and the comparison in terms of nutritional, physical, and sensory properties with a standard formulation was performed. T. molitor incorporation allowed an improvement in the nutritional profile of snacks, through an increase of 15% in protein content and an enrichment in minerals (namely potassium, phosphorus, copper, and zinc). The crackers containing a 6% of insect flour were the most appreciated by the panelists. The incorporation of T. molitor induced a reduction in firmness and an increase in crispness, resulting from the impact of the protein on the structure. This aspect has a positive impact with respect of the acceptance of snacks—70% of the panelists consider the possibility to buy the crackers with 6% enrichment. A darkening of the samples with the increase in the incorporation of T. molitor flour was also observed, accompanied by a reduction of about 20% of the L* values. Globally, insect protein can play an important role in redesigning food diets, making them more sustainable, with less environmental impact and equally balancedinfo:eu-repo/semantics/publishedVersio

Impact of red flour beetle infestations in wheat flour and their effects on dough and bread physical, chemical, and color properties

The impact of Tribolium castaneum on the quality and technological suitability of bread-making wheat flour was evaluated. The aim of this study is to investigate whether insect-infested flours can be used, after pest removal, avoiding the common use of insecticides and flour waste. The tests were carried out with wheat flour infested red flour beetle with 500 adults/kg (N1), 1000 adults/kg (N2) and 2000 adults/kg (N3), for two weeks. Flour color, total starch, protein and water contents, mineral composition and flour acidity were studied. The technological properties of the respective doughs and of bread were characterized. The results showed that infested flours acidity was significantly higher (N1, N2 and N3 = 0.3 g H2SO4/100 g) than the control (0.1 g H2SO4/100 g), the total starch content decrease, from 78 g (control) to 70 g/100 g dm (N3), and protein content did not significantly change, 7 g/100 g (control) to 8 g/100 g (N2); flour infested showed darker greyish tone which colour differences ΔE higher than 5, while the bread crust was lighter; the gelatinization properties of starch were slightly influenced by degree of infestation with gelatinization temperature reduction of about 5◦c. There was not impact on the structure of the doughs from infested flours measured by rheology. The extensibility of the doughs, before and after fermentation, was moderately affected by the insects. For the respective breads, they kept their softness for longer, without significant volume change. These results encourage insect tolerance on grains and derivatives, avoiding chemical toxic insecticides and food waste. This pretends to be a contribution to pest management and decision support systems, prevention, and control of losses, relevant subject to stored products.info:eu-repo/semantics/publishedVersio

Paddy rice stored under hermetic conditions: the effect of relative humidity, temperature and storage time in suppressing Sitophilus zeamais and impact on rice quality

The aim of this study was to analyse the effect of relative humidity in suppressing Sitophilus zeamais, in paddy rice stored under hermetic conditions, during four and seven months, at different average temperatures, as well as the impact on rice quality. Hermetic bags, GrainPro® SuperGrainbag® Farm™, were used to store two rice varieties under three different relative humidities: 67%, 75% and 85% RH, and average temperatures of 14 C, 17 C and 24 C, both monitored by Hobo® Data loggers, with the probe placed inside the bags. CheckpointII Portable O2 and CO2 Gas Analyzer was used to assess gas contents on the top and bottom of each bag. At the end of the trials, paddy samples were collected to estimate water activity (aw). The rheology behaviour of rice pastes prepared with race flour obtained from the different treatments was also evaluated, using a controlled stress rheometer. The results showed that the response of the stored-product insects changes with environmental conditions, O2 and CO2 contents. Other parameters were considered; aw increased with relative humidity and temperature, but decreased with storage time. The relative humidity played an important role, together with the increase of temperature, in suppressing insect populations. A modified atmosphere was naturally produced inside the hermetic bag, under 85% RH, with low O2 and high CO2 contents, at different average temperatures, 14 C and 17 C. These results demonstrated that S. zeamais can survive, but has no progeny. Under the same conditions, but at the higher average temperature of 24 C, S. zeamais attained 100% mortality before producing progeny. The increase on respiration rate, registered by CO2 increase and O2 decrease, for higher RH values, reduced the viscoelastic functions and changed the starch gelatinization point of Indica and Japonica rice. The results obtained showed that storing paddy hermetically, at low relative humidity, did not change atmospheric content and maintained the viscoelastic functions of the rice pastesinfo:eu-repo/semantics/publishedVersio

Antifungal Activity of Benzoquinones Produced by Tribolium castaneum in Maize-Associated Fungi

Tribolium castaneum (Herbst) adults produce 1,4-benzoquinone (BQ), methyl-1,4-benzoquinone (MBQ), and ethyl-1,4-benzoquinone (EBQ). These components are chemical defenses used as repellents and irritants, and BQ has a negative impact on the growth of some fungal species. In this work, the inhibitory and/or lethal effects of these benzoquinones on the development of six fungi identified inmaize, namely Aspergillus flavus, A. fumigatus, A. niger, Fusarium sp., Penicillium sp., and Trichoderma sp., were evaluated. Ten-day-long disk diffusion trials were performed using benzoquinones. The experiments simulated the activity of BQ (B1) or “EBQ +MBQ” (B2) released by 40-day-old insect adults (n = 200), considering a total average release of 45 g per adult. Inhibition halos imposed by benzoquinones on fungal growth showed a significant effect when compared with the controls (water and solvent). Mycelial growth was decreased for all fungi, with the level of response depending on the fungal species. B1 and B2 displayed an inhibitory effect against all fungi, but Trichoderma sp. and A. niger showed rapid recoveries. B2 showed a lethal effect on Penicillium sp. The inhibitory and lethal activities of benzoquinones released by T. castaneum adults may contribute to regulate fungal growth, and understanding their interaction is important to develop innovative control strategiesinfo:eu-repo/semantics/publishedVersio

The Interaction between Tribolium castaneum and Mycotoxigenic Aspergillus flavus in Maize Flour

Tribolium castaneum is one of the most common insect pests of stored products. Its presence makes cereals more susceptible to the spread of the fungi Aspergillus flavus, which may produce mycotoxins. The aim of this work was to evaluate the influence of T. castaneum adults on the development of a mycotoxigenic A. flavus strain in maize flour as well as the influence of this fungus on the insects. Maize flour was exposed to T. castaneum, spores of A. flavus or to both. The results revealed an interaction between T. castaneum and A. flavus as the flour exposed to both organisms was totally colonized by the fungus whereas almost all the insects were killed. Aflatoxin B1 (AFB1) revealed a significantly higher concentration in the flour inoculated with both organisms (18.8 g/kg), being lower when exposed only to A. flavus, suggesting that the presence of insects may trigger fungal development and enhance mycotoxin production. The ability of these organisms to thrive under the same conditions and the chemical compounds they release makes the interaction between them a subject of great importance to maintain the safety of stored maize. This is the first work evaluating the interaction between T. castaneum and A. flavus mycotoxin productioninfo:eu-repo/semantics/publishedVersio

Effectiveness of hermetic containers in controlling paddy rice (Oryza sativa L.) storage insect pests

Naturally infested paddy rice was used to compare the effectiveness of polypropylene bags and hermetic storage containers over 12 months of storage in a warehouse. Insect pest identification as well as the infestation level, percentage of damaged grain, weight loss, and moisture content were evaluated. Five insect species associated with stored rice were identified during the storage period, namely lesser grain borer (Rhyzopertha dominica), red flour beetle (Tribolium castaneum), rice/maize weevil (Sitophilus spp.), angoumois grain moth (Sitotroga cerealella) and flat grain beetle (Cryptolestes ferrugineus). The lesser grain borer was the most predominant species with an average incidence above 70% after twelve months of storage, followed by the rice/maize weevil with an incidence of 17%. When compared to hermetic storage containers, polypropylene bag showed the highest mean infestation level with 233.3 individuals/ kg after six months of storage, representing about 8-fold of the number of insects recorded in hermetic containers after six months of storage. In polypropylene container, the percentage of damaged grain and weight loss increased significantly achieving a maximum of 6.98% and 5.56% respectively, whereas using hermetic containers the highest percentage of damaged grain reached was 3.24% in polyethylene drum and the weight loss was 1.62% in GrainSafe bag. The results from the study show that the use of hermetic storage containers is a green alternative for safe storage of paddy rice, for 12 months without application of pesticides, bringing multiple advantages for smallholder farmers, lever food security and income generation for smallholder farmers and rice milling companiesinfo:eu-repo/semantics/publishedVersio

Modelling Processes and Products in the Cereal Chain

[EN] In recent years, modelling techniques have become more frequently adopted in the field of food processing, especially for cereal-based products, which are among the most consumed foods in the world. Predictive models and simulations make it possible to explore new approaches and optimize proceedings, potentially helping companies reduce costs and limit carbon emissions. Nevertheless, as the different phases of the food processing chain are highly specialized, advances in modelling are often unknown outside of a single domain, and models rarely take into account more than one step. This paper introduces the first high-level overview of modelling techniques employed in different parts of the cereal supply chain, from farming to storage, from drying to milling, from processing to consumption. This review, issued from a networking project including researchers from over 30 different countries, aims at presenting the current state of the art in each domain, showing common trends and synergies, to finally suggest promising future venues for research.The authors would like to acknowledge networking and article processing charge support by COST Action CA15118 (Mathematical and Computer Science Methods for Food Science and Industry).Carvalho, O.; Charalambides, MN.; Djekic, I.; Athanassiou, C.; Bakalis, S.; Benedito Fort, JJ.; Briffaz, A.... (2021). Modelling Processes and Products in the Cereal Chain. Foods. 10(1):1-18. https://doi.org/10.3390/foods10010082S11810

Modelling Processes and Products in the Cereal Chain

ReviewIn recent years, modelling techniques have become more frequently adopted in the field of food processing, especially for cereal-based products, which are among the most consumed foods in the world. Predictive models and simulations make it possible to explore new approaches and optimize proceedings, potentially helping companies reduce costs and limit carbon emissions. Nevertheless, as the different phases of the food processing chain are highly specialized, advances in modelling are often unknown outside of a single domain, and models rarely take into account more than one step. This paper introduces the first high-level overview of modelling techniques employed in different parts of the cereal supply chain, from farming to storage, from drying to milling, from processing to consumption. This review, issued from a networking project including researchers from over 30 different countries, aims at presenting the current state of the art in each domain, showing common trends and synergies, to finally suggest promising future venues for researchinfo:eu-repo/semantics/publishedVersio