Use of intensive rearing systems as back‐up for coastal lagoon aquaculture: an experience with eels, Anguilla anguilla L., in the Comacchio lagoons

. The possibility of using eels from intensive rearing ponds to restock natural basins has been investigated by comparing growth, survival and rate of yellow-to-silver metamorphosis of 'wild type' eels and of eels from an intensive rearing pond. A total of 2243 marked eels were released into a closed natural environment of 22ha; 1254 were wild type (average length 45·6 ± 9·0cm), and 989 from an intensive rearing pond, split by a mechanical grader in two groups. The first group was made of 617 well-growing eels (44·9 ± 3·0cm), the second one of 372 smaller eels (36·9 ± 4·3cm) which had not adapted to the artificial conditions. The starting size being equal the animals grew to the same extent; in some cases the animals previously adapted to the artificial pond grew better than the wild type. The rate of metamorphosis from yellow to silver eel was 28·4% for the wild type eels and 22% for the others, although the latter became mostly silver males. Survival of the wild type eels (starting with the 35-cm size class and covering the period from April to December) was calculated by mark-recapture at approximately 98·6%. For the eels previously well adapted to the intensive rearing pond it was only 85%, indicating some difficulty in competing in the natural environment. The good growth shown by the smaller eels, with a survival of about 90%, would seem to indicate that these animals might be effectively used for restocking purposes, thus favouring an integration between the intensive ponds and the extensive environments

Interleukin-6 and granulocyte macrophage-CSF in the cerebrospinal fluid from HIV infected subjects with involvement of the central nervous system.

We detected the cytokines interleukin-6 (IL-6) and granulocyte macrophage-CSF (GM-CSF) by ELISA in the CSF and serum of 30 HIV-infected patients classified as AIDS dementia complex (ADC), and 20 subjects with other neurological diseases (OND). We have found a high incidence of detectable IL-6 and GM-CSF in the CSF of ADC patients compared with OND patients. No statistical differences were observed between both groups for serum IL-6 and GM-CSF levels. These results suggest an intrathecal synthesis of these cytokines and a possible involvement in the pathogenesis of ADC

Effect of 2 sex-sorting time schedules on SIT facility management

Improvements are needed in mosquito mass-rearing to effectively implement the sterile insect technique (SIT). However, managing this technique is challenging and resource intensive. SIT relies on mass rearing, sterilization, and release of adult males to reduce field populations. Maintaining an acceptable level of female presence, who can transmit viruses through biting, is crucial. Females are also essential for facility sustainability. Sex sorting plays a vital role in the production process, and our current mechanical sorting approach aims to obtain a high number of adult males with minimal female contamination within 24 h of pupation. Utilizing protandry helps control female contamination. While the 24-h sorting period achieves desired contamination levels, it may not yield enough females to sustain breeding lines, leading to increased labor costs that impact project sustainability. By delaying the sorting procedure to 48 h, we obtained sufficient females to sustain breeding lines, achieving a balance between male production and female contamination using the automatic version of the Fay-Morlan device as the sorting tool

Aedes albopictus Sterile Male Production: Influence of Strains, Larval Diet and Mechanical Sexing Tools

The sterile insect technique (SIT) is a biologically based method of pest control, which relies on the mass production, sterilization, and release of sterile males of the target species. Since females can transmit viruses, it is important to develop a mass rearing system to produce a large number of males with a low presence of females. We evaluated the effects of different strains, larval diets and sexing tools on male productivity and residual female presence for the application of SIT against Aedes albopictus. Strains coming from Italy, Germany, Greece, and Montenegro, with different levels of colonization, were reared with three larval diets: IAEA-BY, BLP-B and SLP-BY. Developed pupae were sexed using two different mechanical methods: sieve or Fay-Morlan separator. The results proved that adoption of the Fay-Morlan separator increased the productivity and limited the female presence. The IAEA-BY diet showed the lowest female contamination. Strains with a high number of breeding generations showed a decreased productivity and an increased female presence. Increased female presence was found only in extensively reared strains and only when the sorting operation was conducted with sieves. We hypothesize that extensive colonization may determine a size reduction which limits the sexing tool efficiency itself

The importance of clinic attendance in the first six months on antiretroviral treatment: a retrospective analysis at a large public sector HIV clinic in South Africa

<p>Abstract</p> <p>Background</p> <p>Adherence to care and treatment are essential for HIV-infected individuals to benefit from antiretroviral therapy (ART). We sought to quantify the effects on treatment outcomes of missing visits soon after initiating ART.</p> <p>Methods</p> <p>We analyzed data from HIV-infected patients initiating ART at Themba Lethu Clinic, Johannesburg, South Africa, from April 2004 to August 2008. We used log-binomial regression to evaluate the relative risk of missing visits during the first six months of ART on immunological response and virologic suppression. Cox models were used to evaluate the relationship between missed visits and mortality and loss to follow up over 12 months.</p> <p>Results</p> <p>Of 4476 patients, 65% missed no visits, while 26% missed one visit, 7% missed two and 1.6% missed three or more visits during the first six months on treatment. Patients who missed three or more medical or antiretroviral (ARV) visits had a two-fold increased risk of poor CD4 response by six months, while the risk of failing to achieve virologic suppression by six months increased two- to five-fold among patients who missed two and three or more medical or ARV visits. Adjusted Cox models showed that patients who missed two (HR 2.1; 95% CI: 1.0-4.3) and three or more (HR 4.7; 95% CI: 1.4-16.2) medical visits had an increased risk of death, while those who missed two ARV (HR 3.8; 95% CI: 2.5-5.8) or three or more medical (HR 3.0; 95% CI: 1.1-8.1) visits had an increased risk of loss to follow up.</p> <p>Conclusions</p> <p>Thirty-five percent of patients missed one or more visits in the first six months on treatment, increasing their risk of poorer outcomes. These patients could be targeted for additional adherence counselling to help improve ART outcomes.</p

Vitamina C : una estrategia didáctica polifuncional

El presente trabajo tiene como objetivo contextualizar contenidos curriculares de química: incursionando en el área de bioquímica, como elementos básicos de la formación en química del estudiante; proporcionando información general sobre las propiedades de la vitamina C que permitan al estudiante, como ciudadano, tener libertad de elección frente a la vasta publicidad que se ofrece, entre otros, en los medios masivos de comunicación; aplicando su cultura científica a situaciones de la vida cotidiana y, por lo tanto, valorando la importancia de la educación científica para poder ser capaz de conocer mejor lo que consume y poder opinar en forma crítica sobre ello. Se presentan técnicas sencillas para investigar la presencia de vitamina C en alimentos de uso común. Se discuten distintas estrategias de presentación y tratamiento del tema en los distintos niveles escolares con objetivos diferentes y se analizan cambios en la metodología de trabajo en el aula. Este trabajo fue presentado bajo la modalidad de taller en el XV Congreso Nacional e Internacional de Profesores de Química y los resultados y conclusiones a las que se arribaron fueron ampliamente satisfactorias, ya que permitieron una revalorización de la tarea educativa buscando un currículo más abierto donde el estudiante se sienta partícipe de su proceso de aprendizaje y encuentre contenidos científicos útiles para su vida.This paper attempts to contextualise chemistry curricula contents, entering the field of biochemistry, as basic elements for the formation of chemistry in the student by providing general information on the properties of C vitamin that allows the student, as a citizen, the freedom to choose in front of the huge publicity offered to them, among others, by the mass media. They can apply their scientific culture to everyday life, thus assessing how important scientific education is to be able to better know what is consumed and give a critical opinion. We present simple techniques to find C vitamin in commonly used food. We discuss different strategies to present and deal with this subject at different school levels with different aims, and we analyse the changes in the classroom working methodology. This paper was presented as a workshop in the 15th National and International Congress of Chemistry Professors and its results and conclusion were highly satisfactory, as they allowed revaluing the educational task aiming at a more open curriculum where the student feels part of his learning process and finds scientific contents useful in his everyday life

\u201cWhat is more important than love?\u201d. Parental attachment and romantic relationship in Italian emerging adulthood

Previous researches suggest that individuals with different attachment styles practice different styles of love, but these do not consider the role of trust, communication, and closeness to the father and mother separately. The main aim of this study was to evaluate the relationship and the impact of parental attachment, through the analysis of the participants\u2019 self-reported account and 1. Department of Education, Cultural Heritage and Tourism, University of Macerata. Postbox: Piazzale Luigi Bertelli (Contrada Vallebona) 62100, Macerata, Italy 2. Psychology of Communication department, University of Macerata, Angelo Carrieri. 3. University of Pablo de Olavide, (ES), Health Plus Parish Priest Mifsud Str. Hamrun, Malta 4. Health Plus Parish Priest Mifsud Str. Hamrun, Malta Accepted Manuscript 4 romantic styles in Italians emerging adulthood by using a multidimensional approach (trust, communication, closeness to father and mother). The 296 participants (19\u201329 years; 50.7% males) rated items of information on a questionnaire, regarding their perspective of their attachment to their mother/father and attitude toward love. Using a variable-centred approach and a person-centred approach, the results suggest that the respondents differed in levels of parental attachment or love styles and that the present parental attachment has a positive impact on their romantic relationship. It is possible to estimate romantic relationships and prevent manic relationships based on the individual\u2019s current perceptions of their attachment to the father or mother. The role of parents and paternal attachment, are still fundamental in Italian young adults. The role of communication with the mother, in particular, is controversial and should be further investigated

Insufficient access to harm reduction measures in prisons in 5 countries (PRIDE Europe): a shared European public health concern

Background: Prisoners constitute a high-risk population, particularly for infectious diseases. The aim of this study was to estimate the level of infectious risk in the prisons of five different European countries by measuring to what extent the prison system adheres to WHO/UNODC recommendations. Methods: Following the methodology used in a previous French survey, a postal/electronic questionnaire was sent to all prisons in Austria, Belgium, Denmark and Italy to collect data on the availability of several recommended HIV-HCV prevention interventions and HBV vaccination for prisoners. A score was built to compare adherence to WHO/UNODC recommendations (considered a proxy of environmental infectious risk) in those 4 countries. It ranged from 0 (no adherence) to 12 (full adherence). A second score (0 to 9) was built to include data from a previous French survey, thereby creating a 5-country comparison. Results: A majority of prisons answered in Austria (100 %), France (66 %) and Denmark (58 %), half in Belgium (50 %) and few in Italy (17 %), representing 100, 74, 89, 47 and 23 % coverage of the prison populations, respectively. Availability of prevention measures was low, with median adherence scores ranging from 3.5 to 4.5 at the national level. These results were confirmed when using the second score which included France in the inter-country comparison. Overall, the adherence score was inversely associated with prison overpopulation rates (p = 0.08). Conclusions: Using a score of adherence to WHO/UNODC recommendations, the estimated environmental infectious risk remains extremely high in the prisons of the 5 European countries assessed. Public health strategies should be adjusted to comply with the principle of equivalence of care and prevention with the general community