Partitions of the set of finite sequences

AbstractWe consider partitions of the set of finite sequences of a cardinal and investigate the existence of different kinds of homogeneous objects for them. We prove some properties of the first cardinal satisfying a natural partition relation and study how different types of homogeneitics are interrelated

SeaClouds: An Open Reference Architecture for Multi-Cloud Governance

A. Brogi, J. Carrasco, J. Cubo, F. D'Andria, E. Di Nitto, M. Guerriero, D. Pérez, E. Pimentel, J. Soldani. "SeaClouds: An Open Reference Architecture for Multi-Cloud Governance". In B. Tekinerdogan et al. (Eds.): ECSA 2016, LNCS 9839, pp. 334–338, 2016.We present the open reference architecture of the SeaClouds solution. It aims at enabling a seamless adaptive multi-cloud management of complex applications by supporting the distribution, monitoring and reconfiguration of app modules over heterogeneous cloud providers.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

The Wonder of Colors and the Principle of Ariadne

The Principle of Ariadne, formulated in 1988 ago by Walter Carnielli and Carlos Di Prisco and later published in 1993, is an infinitary principle that is independent of the Axiom of Choice in ZF, although it can be consistently added to the remaining ZF axioms. The present paper surveys, and motivates, the foundational importance of the Principle of Ariadne and proposes the Ariadne Game, showing that the Principle of Ariadne, corresponds precisely to a winning strategy for the Ariadne Game. Some relations to other alternative. set-theoretical principles are also briefly discussed

Protective Effects of Human and Mouse Soluble Scavenger-Like CD6 Lymphocyte Receptor in a Lethal Model of Polymicrobial Sepsis

Sepsis still constitutes an unmet clinical need, which could benefit from novel adjunctive strategies to conventional antibiotic therapy. The soluble form of the scavenger-like human CD6 lymphocyte receptor (shCD6) binds to key pathogenic components from Gram-positive and -negative bacteria and shows time- and dose-dependent efficacy in mouse models of monobacterial sepsis. The objective of the present work was to demonstrate the effectiveness of infusing mouse and human sCD6 by different systemic routes, either alone or as adjunctive therapy to gold standard antibiotics, in a lethal model of polymicrobial sepsis. To this end, C57BL/6 mice undergoing high-grade septic shock induced by cecal ligation and puncture (CLP; ≥90% lethality) were infused via the intraperitoneal (i.p.) or intravenous (i.v.) route with shCD6 at different doses and time points, either alone or in combination with imipenem/cilastatin (I/C) at a dose of 33 mg/kg of body weight every 8 h. Significantly reduced mortality and proinflammatory cytokine levels were observed by i.p. infusion of a single shCD6 dose (1.25 mg/kg) 1 h pre- or post-CLP. When using the i.v. route, mice survival was significantly extended by starting shCD6 infusion at later time points post-CLP (up to 6 h after CLP). Significant adjunctive effects on mouse survival were observed by i.p. or i.v. infusion of shCD6 in combination with i.p. I/C post-CLP. Similar results were obtained in mice expressing high sustained levels (5 to 10 μg/ml) of mouse sCD6 in serum by means of transduction with hepatotropic adeno-associated virus (AAV). Taken together, the data support the conserved antibacterial effects of human and mouse sCD6 and their use as adjunctive therapy in experimental models of complex and severe polymicrobial sepsis.Ministerio de Economía y Competitividad (España) SAF2013-46151-R PCIN-2015-070Instituto de Salud Carlos III RD12/0015/0018European Development Regional Fund RD12/0015/0018Fundació La Marató TV3 201319-30-3

Investigation of VvMybA1 and VvMybA2 berry color genes in ‘Aglianico’ biotypes

Research Not

Formación en investigación educativa en la sociedad digital. Una experiencia innovadora de enseñanza en el nivel superior en el contexto latinoamericano

From the Educational Research 2 Chair, at Universidad Nacional de San Luis, Argentina, we wonder about content relevance and value of teaching strategies used in the education of future educational professionals and we assume the challenge to rethink the discipline and its teaching methodology, in today's society context.We analized research from new technologies aspects and digital development. The expression it takes in Latin American context was analyzed. We applied an innovative work experience to fourth year students of Educational Sciences, with a focus on student-centered teaching and actions that arise from flipped learning, extended learning and collaborative work principles.The article explores the relationship established between educational research, technology and educational research; and it promotes reflection on the impact it may have on the contents of training researchers, training processes, profile of researchers and educational research itself. The article describes the principles which originate the experience, the way we worked, the more valued and critical aspects considered or those which will require more attention and study. The article finishes with some general thoughts.Desde la Cátedra Investigación Educativa II de la Universidad Nacional de San Luis, Argentina, nos preguntamos acerca de la pertinencia de los contenidos y valor de las estrategias de enseñanza que se utilizan para formar en investigación a los futuros profesionales de la educación y asumimos el reto de repensar la disciplina misma y su enseñanza en el contexto de la sociedad actual.Se analizó la investigación a la luz de las nueva tecnologías y el desarrollo de lo digital, se indagó la expresión que esto adopta en el contexto latinoamericano y se planificó una experiencia de trabajo innovadora para los estudiantes de cuarto año del Profesorado y la Licenciatura en Ciencias de la Educación, con un enfoque de enseñanza centrado en el estudiante, y acciones que devienen de los principios del aprendizaje invertido, aprendizaje extendido y trabajo colaborativo.El artículo profundiza la relación que se establece entre investigación educativa, tecnología y enseñanza de la investigación; y promueve la reflexión en torno al impacto que la misma puede tener sobre los contenidos de la formación de investigadores, los procesos de formación, el perfil de los investigadores y la investigación educativa misma. Relata los principios de los que se partió en la experiencia, el modo como se trabajó, los aspectos más valorados y los considerados críticos o que requieren más atención y estudio. Cierra con unas reflexiones generales

Disulfide Engineered Lipase to Enhance the Catalytic Activity: A Structure-Based Approach on BTL2

Enhancement, control, and tuning of hydrolytic activity and specificity of lipases are major goals for the industry. Thermoalkaliphilic lipases from the I.5 family, with their native advantages such as high thermostability and tolerance to alkaline pHs, are a target for biotechnological applications. Although several strategies have been applied to increase lipases activity, the enhancement through protein engineering without compromising other capabilities is still elusive. Lipases from the I.5 family suffer a unique and delicate double lid restructuration to transition from a closed and inactive state to their open and enzymatically active conformation. In order to increase the activity of the wild type Geobacillus thermocatenulatus lipase 2 (BTL2) we rationally designed, based on its tridimensional structure, a mutant (ccBTL2) capable of forming a disulfide bond to lock the open state. ccBTL2 was generated replacing A191 and F206 to cysteine residues while both wild type C64 and C295 were mutated to serine. A covalently immobilized ccBTL2 showed a 3.5-fold increment in esterase activity with 0.1% Triton X-100 (2336 IU mg-1) and up to 6.0-fold higher with 0.01% CTAB (778 IU mg-1), both in the presence of oxidizing sulfhydryl agents, when compared to BTL2. The remarkable and industrially desired features of BTL2 such as optimal alkaliphilic pH and high thermal stability were not affected. The designed disulfide bond also conferred reversibility to the enhancement, as the increment on activity observed for ccBTL2 was controlled by redox pretreatments. MD simulations suggested that the most stable conformation for ccBTL2 (with the disulfide bond formed) was, as we predicted, similar to the open and active conformation of this lipase.Financial and logistic support from Colombian Universidad del Valle and COLCIENCIAS (CI 71083-Grant 745-2016-Project 110671250425), Spanish CICYT project BIO-2005-6018576, BFU2017-90030-P, and BFU2011-25326, B. Di G. In addition, thanks to the Spanish MINECO for a FPU fellowship.S

The Gaia spectrophotometric standard stars survey -II. Instrumental effects of six ground-based observing campaigns

The Gaia SpectroPhotometric Standard Stars (SPSS) survey started in 2006, it was awarded almost 450 observing nights, and accumulated almost 100,000 raw data frames, with both photometric and spectroscopic observations. Such large observational effort requires careful, homogeneous, and automated data reduction and quality control procedures. In this paper, we quantitatively evaluate instrumental effects that might have a significant (i.e.,$\geq$1%) impact on the Gaia SPSS flux calibration. The measurements involve six different instruments, monitored over the eight years of observations dedicated to the Gaia flux standards campaigns: DOLORES@TNG in La Palma, EFOSC2@NTT and ROSS@REM in La Silla, [email protected] in Calar Alto, BFOSC@Cassini in Loiano, and [email protected] in San Pedro Martir. We examine and quantitatively evaluate the following effects: CCD linearity and shutter times, calibration frames stability, lamp flexures, second order contamination, light polarization, and fringing. We present methods to correct for the relevant effects, which can be applied to a wide range of observational projects at similar instruments.Comment: 16 pages, 13 figures, accepted for publication in Astron. Nach

Clinical research during the year of obligatory practice : our second year of experience

Introducción: Es conocida la preocupación mundial por la disminución de los médicos-investigadores. La investigación permite el desarrollo de destrezas como búsqueda bibliográfica, análisis reflexivo de información y contribuye a mejorar habilidades diagnósticas y terapéuticas. Nuestro curriculum incluye cursos como epidemiología y bioestadística, que promueven la adquisición de destrezas para la realización de un proyecto de investigación, pero pocos llegan a ejecutarse. Objetivo: Promover en los estudiantes de la carrera de medicina una actitud positiva hacia la investigación. Desarrollo del proyecto: En 2016 se sumó como actividad obligatoria de los estudiantes de PFO, el diseño de un proyecto de investigación. Luego un curso de metodología de investigación, los estudiantes elaboraron 4 proyectos, con la supervisión de tutores, trabajando en foros virtuales y reuniones presenciales. Los trabajos fueron llevados a campo y en tres de ellos se realizó un análisis estadístico sencillo y se obtuvieron conclusiones que fueron presentadas frente a compañeros de curso, docentes y autoridades. En 2017, luego del curso de metodología de la investigación, los alumnos se dividieron en 10 grupos. Cada grupo desarrolló un proyecto, supervisado por un tutor a través de reuniones presenciales y de un espacio en la plataforma Moodle. También se dictaron charlas sobre “Búsqueda bibliográfica" y “Lenguaje académico en la presentación de trabajos científicos". Los 10 trabajos fueron llevados a campo, se realizó análisis de datos, se elaboraron conclusiones y fueron presentados en una “Jornada de investigación de PFO". Conclusión: Esta experiencia formativa permitió a los estudiantes de la PFO tener la posibilidad de participar en un trabajo de investigación, desarrollando habilidades nuevas. Consideramos que es importante estimular a los estudiantes de medicina a que participen en este tipo de actividades asignando un lugar en distintos espacios curriculares, tiempo, recursos económicos y tutores capacitados para superar las limitaciones actuales.Introduction: It is known, the general concern about the decrease of the number of doctor-researchers. The research allows the development of skills such as bibliographic search, reflective analysis of information and contributes to improve diagnostic and therapeutic skills. Our curriculum includes courses such as epidemiology and biostatistics, which promote the acquisition of skills for the prosecution of a research project, but few are actually executed. Objective: To promote a positive attitude towards research in medical students. Project development: In 2016, it was added the design of a research project as a mandatory activity for PFO students. After a course in research methodology, the students developed four projects, with the supervision of tutors, working in virtual forums and face-to-face meetings. The works were put into practice. In three of them, a simple statistical analysis was carried out and conclusions were obtained and presented to classmates, teachers and authorities. In 2017, after the research methodology course, the students were divided into ten groups, developing ten projects, supervised by a tutor through face-to-face meetings and virtual forums. Lessons about "Bibliographic search" and "Academic language in the presentation of scientific works" were also given. All the proyects were put into practice. Data analysis was carried out, conclusions were drawn up and presented to classmates, teachers and authorities. Conclusion: This formative experience allowed the students of the PFO to participate in a research work, developing new skills. We believe it is important to encourage medical students to participate in this kind of activities assigning a place in the curriculum, time, financial resources and trained tutors to overcome current limitationsFil: Di Lorenzo, Gabriela. Universidad Nacional de Cuyo. Facultad de Ciencias MédicasFil: Da Costa Marques, Sofía Beatriz. Universidad Nacional de Cuyo. Facultad de Ciencias Políticas y SocialesFil: Echeverría, María Inés. Universidad Nacional de Cuyo. Facultad de Ciencias MédicasFil: Vega, Israel. Universidad Nacional de Cuyo. Facultad de Ciencias MédicasFil: Pennisi, Alicia. Universidad Nacional de Cuyo. Facultad de Ciencias MédicasFil: Carrasco, Norma. Universidad Nacional de Cuyo. Facultad de Ciencias MédicasFil: Biliato, Luis. Universidad Nacional de Cuyo. Facultad de Ciencias Médica